JP2002238598A5 - - Google Patents
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- JP2002238598A5 JP2002238598A5 JP2001037996A JP2001037996A JP2002238598A5 JP 2002238598 A5 JP2002238598 A5 JP 2002238598A5 JP 2001037996 A JP2001037996 A JP 2001037996A JP 2001037996 A JP2001037996 A JP 2001037996A JP 2002238598 A5 JP2002238598 A5 JP 2002238598A5
- Authority
- JP
- Japan
- Prior art keywords
- phospholipase
- measuring
- acid
- reagent composition
- calcium ions
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【特許請求の範囲】
【請求項１】
少なくともホスフォリパーゼＤ、ホスフォリパーゼＤの基質、グリコールエーテルジアミン四酢酸、およびジエチレントリアミン五酢酸を含有することを特徴とするカルシウムイオン測定用試薬組成物。
【請求項２】
さらにリン酸モノエステラーゼを含有することを特徴とする、請求項１に記載のカルシウムイオン測定用試薬組成物。
【請求項３】
少なくともホスフォリパーゼＤ、ホスフォリパーゼＤの基質、グリコールエーテルジアミン四酢酸、およびジエチレントリアミン五酢酸を含有するカルシウムイオン測定試薬組成物にカルシウムイオンを含有する試料溶液を添加した後、ホスフォリパーゼＤ生成物を測定することを特徴とするカルシウムイオンの測定方法。
【請求項４】
該カルシウムイオン測定試薬組成物が、さらにリン酸モノエステラーゼを含有することを特徴とする、請求項３に記載のカルシウムイオンの測定方法。
【請求項５】
ホスフォリパーゼＤをグリコールエーテルジアミン四酢酸およびジエチレントリアミン五酢酸処理することを特徴とする、カルシウムイオン測定用試薬組成物におけるホスフォリパーゼＤのカルシウムイオンに対する特異性を高める方法。
【請求項６】
ホスフォリパーゼＤをグリコールエーテルジアミン四酢酸およびジエチレントリアミン五酢酸処理することを特徴とする、カルシウムイオンの測定方法におけるホスフォリパーゼＤのカルシウムイオンに対する特異性を高める方法。
【請求項７】
請求項５に記載の方法を利用することを特徴とする、試料中のカルシウムイオンの測定方法。
【請求項８】
ホスフォリパーゼＤをグリコールエーテルジアミン四酢酸およびジエチレントリアミン五酢酸処理した水溶液に、マグネシウムイオンを共存させることを特徴とする、カルシウムイオン測定用試薬組成物の長期保存安定化方法。
[Claims]
(1)
A reagent composition for measuring calcium ion, comprising at least phospholipase D, a substrate of phospholipase D, glycol ether diaminetetraacetic acid, and diethylenetriaminepentaacetic acid.
(2)
The calcium ion measurement reagent composition according to claim 1, further comprising a phosphate monoesterase.
(3)
After adding a sample solution containing calcium ions to a calcium ion measurement reagent composition containing at least phospholipase D, a substrate of phospholipase D, glycol ether diamine tetraacetic acid, and diethylenetriaminepentaacetic acid, the formation of phospholipase D A method for measuring calcium ions, comprising measuring an object.
(4)
The calcium ion measuring method according to claim 3, wherein the calcium ion measuring reagent composition further contains a phosphate monoesterase.
(5)
A method for increasing the specificity of phospholipase D for calcium ions in a reagent composition for measuring calcium ions, which comprises treating phospholipase D with glycol ether diaminetetraacetic acid and diethylenetriaminepentaacetic acid.
6.
A method for increasing the specificity of phospholipase D for calcium ions in a method for measuring calcium ions, which comprises treating phospholipase D with glycol ether diaminetetraacetic acid and diethylenetriaminepentaacetic acid.
7.
A method for measuring calcium ions in a sample, comprising using the method according to claim 5.
Claim 8.
A long-term storage and stabilization method for a calcium ion measurement reagent composition, wherein magnesium ion is allowed to coexist in an aqueous solution obtained by treating phospholipase D with glycol ether diamine tetraacetic acid and diethylene triamine pentaacetic acid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001037996A JP4577863B2 (en) | 2001-02-15 | 2001-02-15 | Composition for measuring calcium ion and measuring method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001037996A JP4577863B2 (en) | 2001-02-15 | 2001-02-15 | Composition for measuring calcium ion and measuring method |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002238598A JP2002238598A (en) | 2002-08-27 |
| JP2002238598A5 true JP2002238598A5 (en) | 2007-12-27 |
| JP4577863B2 JP4577863B2 (en) | 2010-11-10 |
Family
ID=18901050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001037996A Expired - Fee Related JP4577863B2 (en) | 2001-02-15 | 2001-02-15 | Composition for measuring calcium ion and measuring method |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4577863B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100354630C (en) * | 2002-10-31 | 2007-12-12 | 世诺临床诊断制品株式会社 | Reagent for testing calcium in sample and test method thereof |
| JP5614281B2 (en) * | 2008-04-14 | 2014-10-29 | 和光純薬工業株式会社 | Phenyl phosphorylcholine derivative |
| JP5244477B2 (en) * | 2008-07-08 | 2013-07-24 | 関東化学株式会社 | Calcium measurement reagent avoiding the influence of gadolinium |
| JP5880625B2 (en) * | 2014-06-13 | 2016-03-09 | 栗田工業株式会社 | Method and apparatus for measuring heavy metal collector concentration |
| JP6649309B2 (en) * | 2017-03-23 | 2020-02-19 | 株式会社東芝 | Water treatment system and working medium |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2899061B2 (en) * | 1990-05-18 | 1999-06-02 | 協和メデックス株式会社 | Calcium determination method |
| JPH0491798A (en) * | 1990-08-02 | 1992-03-25 | Serotetsuku:Kk | Reagent composition for each determinating magnesium and calcium |
| JP3164165B2 (en) * | 1992-02-07 | 2001-05-08 | オリエンタル酵母工業株式会社 | Calcium determination method |
| JPH07170999A (en) * | 1993-12-20 | 1995-07-11 | Unitika Ltd | Reagent for analysis of calcium ion |
-
2001
- 2001-02-15 JP JP2001037996A patent/JP4577863B2/en not_active Expired - Fee Related
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