JP2002191395A - Method for discriminating genuine product from false product - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a marker agent to easily discriminate a genuine product from a false product, a false product detecting agent and to provide a method for the use of the detecting agent. SOLUTION: A marker agent containing at least one substance selected from a chemoluminescent substrate, a bioluminescent substrate and their derivatives is (1) attached to a fiber or cloth and a genuine product is produced by using the marked fiber, or the like, or (2) attached to a genuine metall fitting to be attached to a genuine product. A false product detecting agent containing luciferase and ATP is applied to the genuine product, the product is irradiated with light and the emission of fluorescence or luminance is detected to ascertain the genuineness of the product.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a method of attaching a marker agent to products such as sewn products, leather goods, accessories and the like manufactured by a famous manufacturer, etc. The present invention relates to a fake detection marker agent and a fake detection agent used for determining whether or not the product is a genuine product, a method of determining the authenticity and a method of preventing imitation using the same.

[0002]

2. Description of the Related Art Bags, wallets,
Clothes, sewn products, leather goods, accessories, etc. are marked with the mark of the famous manufacturer, thereby identifying or indicating the source of the product and other products, and thereby ensuring quality assurance. And also promotes advertising. On the other hand, good wiil products of such famous manufacturers
In order to make a profit by piggybacking on l, a counterfeiter may elaborate a mark of a well-known manufacturer and sell a counterfeit product with the counterfeit mark. Imitation or counterfeiting techniques of counterfeiters have become increasingly sophisticated, and the average consumer has been unable to distinguish between genuine and counterfeit products, resulting in devastating damage.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a marker agent, a fake detection agent and a method for using the same, which are used to easily identify authentic products. The present inventor has conducted intensive studies to achieve the above object, and as a result, a marker agent containing, as an active ingredient, at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate, and a derivative thereof, as a fiber or cloth. Adhering, producing a genuine product using the fiber or cloth, and reacting the genuine product with at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and derivatives thereof to obtain luminescence or fluorescence. By applying a fake detection agent containing the compound to be obtained and observing whether or not fluorescence or luminescence can be obtained, it was found that the authenticity of the product could be easily determined, and the present invention was completed based on this finding. I came to.

[0004]

Thus, according to the present invention, there is provided a marker agent or fake detection agent containing, as an active ingredient, at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof. Provided is a fake detection agent or marker agent containing a compound capable of reacting with at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate or a derivative thereof to obtain luminescence or fluorescence.

Further, according to the present invention, a marker agent is attached to a fiber or a cloth, a genuine product is manufactured using the fiber or the cloth, or the marker agent is attached to a metal fitting to be attached to the genuine product, Applying a fake detection agent containing a compound capable of reacting with the marker agent to a product on the market, and detecting a reaction product of the marker agent and the fake detection agent or a property derived from the reaction product. A method for determining whether or not a product circulating in the market is authentic; shipping and / or inventory management of the marker agent, and shipping and / or inventory of a fiber, cloth or metal fitting to which the marker agent is attached; It manages and controls the marker agent and the fiber, cloth or metal fittings to which the marker agent is attached so that it is not used for purposes other than genuine products. The fake detection agent containing a compound obtained fluorescence was applied, by obtaining a fluorescence or luminescence,
A method for discriminating the authenticity of the product and preventing the distribution of a counterfeit product; and adhering the marker agent and a compound capable of reacting with the marker agent to obtain luminescence or fluorescence to a fiber or cloth, Manufacture a genuine product using, or attach a compound capable of reacting with the marker agent and the marker agent to obtain luminescence or fluorescence to a metal fitting to be attached to the product, and irradiating light to products on the market, A method is provided for determining whether a product on the market is authentic based on whether light emission or fluorescence is obtained.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION The marker agent for detecting fake of the present invention contains at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof as an active ingredient.

[0007] The active ingredient of the marker agent of the present invention is a luminescent substrate. A luminescent substrate is a substance that can emit or emit fluorescence by a chemical or biological reaction. Chemiluminescent substrates include, for example, anthracene and its derivatives, and bioluminescent substrates include, for example, luciferin, its derivatives and precursors. Luciferins that can be used as the active ingredient include luminescent organisms, such as luminescent bacteria,
Those derived from sea fireflies, light mushrooms, luminous insects, luminous shrimp and the like can be mentioned. Examples of the luminescent insects include those belonging to the beetles (Coleoptera), for example, insects of the family Firefly and click beetle, specifically, fireflies, firefly, American fireflies, Russian fireflies, light click beetles, flutterflys, and railway insects. . Luciferin derived from insects of the firefly family is light,
In particular, bright yellow-green fluorescence is generated by irradiation with ultraviolet rays. Luciferin emits yellow-green light or the like when it is brought into contact with luciferase, ATP and metal ions. The color of light emission can be changed depending on the type of luciferase used. Luciferin is easily available because it is produced industrially. From the above,
Luciferin is suitable as an active ingredient of the marker agent of the present invention. The luciferin derivative means the above-mentioned luciferin salt or chemically modified luciferin. Also,
The luciferin derivative referred to in the present invention also includes a compound capable of expressing fluorescence and luminescence by undergoing a chemical change or the like.

The luciferin derivatives include D-luciferin-O-sulfate (D-luciferin-O-sulfate).
ulphate), D-luciferin methyl ester (D-luciferin methylester)
r), D-luciferin-O-phosphate (D-luci)
ferin-O-phosphate), D-luciferin-L-phenylalanine (D-luciferin)
-L-phenylalanine), D-luciferin-L-Nα-arginine (D-luciferin-
L-Nα-arginine).

As the luciferin precursor, 2-cyano-6-methoxybenzothiazole (2-Cyano-6) is used.
-Methyoxybenzothiazol), D-cysteine and the like. In addition, the active ingredient contained in the marker agent may be one kind, or two or more kinds. Further, the marker agent may be composed of only the above-mentioned active ingredient, and other components, for example, excipient, dispersant, emulsifier, preservative, preservative, pH
It may contain a preparation, a hygroscopic agent, a surfactant, a pigment, a dye, a paint, a thickener, an adhesive and the like. The type of the other components, the mixing ratio with the active ingredient, and the like are not particularly limited, and may be appropriately set according to the operability of the marker agent and the like. The concentration of luciferin in the marker agent is not particularly limited,
As an optimum concentration, 0.01 mM or more, preferably 0.0 mM
The concentration is 3-50 mM, most preferably 0.10-12 mM. The marker agent can be attached to a carrier. As the carrier, a carrier of any shape (bead, plate, porous, etc.) made of synthetic polymer, silica gel, glass, plastic, metal or the like can be used. In the present invention, a fiber or cloth for manufacturing a product,
Alternatively, a marker agent is attached to a metal fitting to be attached to a product.

[0010] The fake detection agent of the present invention contains a compound capable of reacting with at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate or a derivative thereof to obtain luminescence or fluorescence. The compound capable of reacting with the luminescent substrate to obtain luminescence or fluorescence is not particularly limited, but luciferin, luciferin derivative, luciferin precursor, anthracene or anthracene derivative contains luciferase and ATP, and further contains a metal salt as necessary. Is preferred. Luciferase includes Luciola cruciat (Luciola cruciat).
a), Luciola lateris
eralis), Luciola Minglerica (Lucio)
la mingrelica, Lampyris nactilca, Photinus pyraris
s), Photuri Pennsylvanica (Photouri)
s pennsylvanica), Hotaria parvula, Pyrocoelia miyako,
Pyrophorus plagioftaramus
and luciferases derived from beetles (Coleoptera) such as S. plagiophthalamus, luciferases obtained by producing these luciferases and mutant luciferases by a gene recombination method, and luciferases derived from other non-beetles.

ATP means adenosine 5'-triphosphate, a salt thereof, and adenosine 5'-triphosphate-producing reaction system. The adenosine 5'-triphosphate production reaction system includes a method of producing phosphoenol pyruvate and adenosine 5'-diphosphate by catalyzing them with pyruvate kinase, and a method of producing creatine phosphate and adenosine 5'-diphosphate. A method of producing by catalyzing with creatine kinase, and other methods.

Examples of the metal salt include divalent metal salts, for example, magnesium salts such as magnesium sulfate and magnesium chloride, and manganese salts such as manganese sulfate and manganese chloride. In the present invention, a buffer, an enzyme stabilizer and the like can be contained in addition to the above-mentioned components. The buffer is preferably one that keeps the luminescence reaction solution within the optimal pH range of luciferase, and includes any buffer such as glycine-NaOH buffer, HEPES buffer, and Tris buffer. When this buffer (buffer solution) is added, an effect of remarkably maintaining luminescence is exerted. Examples of the enzyme stabilizer include sugars such as sucrose and glycerol. When this enzyme stabilizer is added, the effect of remarkably maintaining luminescence is exerted.

The amount of luciferase, ATP and metal salt,
Further, the amounts of the buffer and the enzyme stabilizer are not particularly limited.
The concentration of luciferase is usually 0.015 mg / ml or more, preferably 0.05 to 20.0 mg / ml, and most preferably 0.15 to 5 mg / ml. ATP
Is usually 0.002 mM or more, preferably 0.02 mM or more.
2-50 mM, and most preferably 0.2-20 mM
It is. The concentration of the metal salt is usually at least 0.02 mM, preferably 0.1-300 mM, and most preferably 0.5 mM.
１００100 mM. The pH of the buffer (buffer) is usually from 5.0 to 11.0, preferably from 6.0 to 10.0, and most preferably from 7.0 to 9.0. In particular, when glycine-NaOH (buffer) (pH 7.5) is used, it is preferably used at a concentration of 0.01 mM or more, preferably 1 mM to 2.0 M, and most preferably 0.1 to 1.0 M. . The concentration of the enzyme stabilizer is usually 0.1 mM or higher, preferably 1 to 300 mM, and most preferably 10 to 5 mM.
0 mM.

In the present invention, the substance constituting the marker agent and the substance constituting the counterfeit detection agent may be used in a pair, and a substance having the same composition as the marker agent is used as the counterfeit detection agent. Those having the same composition as the detection agent can be used interchangeably as a marker agent.

[0015] The product authenticity discrimination method of the present invention comprises the steps of: (1) attaching a marker agent to a fiber or cloth and manufacturing a genuine product using the fiber or cloth; or (2) a metal fitting to be attached to the genuine product. A marker detection agent containing a compound capable of reacting with the marker agent is applied to products on the market, and a reaction product of the marker agent and the counterfeit detection agent is detected. including. The method of attaching the marker agent to the fiber or cloth or the metal fitting is not particularly limited, and a method of directly applying or dipping the liquid of the marker agent; making the marker agent contain an adhesive made of rubber latex, resin dispersion, or the like; Is applied to a fiber or cloth and dipped.

A genuine product is manufactured under the control and supervision of a genuine manufacturer. In the method of the present invention, this authentic product is produced from a fiber or cloth to which the marker agent has been attached. Alternatively, the marker agent is attached to a fitting to be attached to a genuine product manufactured in a normal process, for example, a fitting such as a fastener, a mark, a screw, or the like, or to a genuine product body. For the marker agent to be attached to the genuine product, the marker agent manufacturer controls the shipping amount and / or the inventory amount, and the fiber or cloth manufacturer or the metal fittings manufacturer to which the marker agent is attached is the marker agent usage amount and / or inventory. The quantity is controlled so that the marker agent is not used for purposes other than genuine use. In the method of the present invention, the marker agent is not particularly limited, but preferably contains the luminescent substrate as an active ingredient. Shipping and / or inventory management of the marker agent, fiber, cloth or metal fittings may be a conventional ledger management method or a method using a data mining method.

Further, the manufacturer of the fiber or the cloth or the manufacturer of the metal fittings manages the shipment amount and / or the stock amount of the fiber or the cloth or the metal fittings to which the marker agent is attached, and the user of the fiber or the cloth or the user of the metal fittings. Manages the usage amount and / or stock amount of the fiber, cloth or metal fitting, and manages the fiber, cloth or metal fitting to which the marker agent is attached so as not to be used for purposes other than genuine use. Shipping and inventory management of the marker agent, fiber or cloth may be a conventional ledger management method or a method using a data mining method. In addition, the above manufacturers and others jointly establish a manager or corporation (hereinafter simply referred to as a management corporation) that manages the shipment and inventory status of marker agent manufacturers, textile manufacturers, cloth manufacturers, product manufacturers, etc. collectively.
Shipping, use and inventory may be jointly managed. In this case, each manufacturer sends data such as the amount of shipment to the management corporation, and the management corporation monitors the balance between the amount of shipment, the amount of use, and the amount of stock, and supervises that there is no use other than genuine products.

Specified marker agents are attached to genuine products shipped in a controlled state as described above. The genuine product is coated with the fake detection agent. The marker agent and the fake detection agent react only in a specific combination, and do not react when the type of the marker agent and the type of the fake detection agent are different from those in the specific combination. Also, even if a reaction occurs, it reacts differently (for example, the emission color is different) from when it was made to react to a genuine product.
This can also be determined. Therefore, when a genuine product is coated with a specified fake detection agent, it reacts with a specified marker agent. In the present invention, a fluorescent or luminescent substance is generated by the reaction between the marker agent and the fake detection agent, and emits fluorescence or light. It can be seen that those that emit fluorescence or light are genuine, and those that do not emit fluorescence are light.

When used in combination of a marker / fake detection agent comprising a combination of a luminescent substrate and a compound which emits or fluoresces it, both of them may be used as authentic products, or fibers or cloths or metal fittings as described above. It is also possible to determine the authenticity of the product based on whether light emission or fluorescence is obtained by irradiating the attached portion with light.

[0020]

EXAMPLE A 1 mM aqueous firefly luciferin aqueous solution (manufactured by Sigma) was added to a urethane resin dispersion to obtain a fiber treating agent. Acrylic fibers were immersed in the fiber treating agent and dried. Weaving the acrylic fiber together with unimmersed acrylic fiber,
They sewed them to make authentic bags. On the surface of the bag, a red emission color mutant firefly luciferase (Kajiyama, N. et. Al., Pro
tein Engineering, 4,691-693 (1991)) and an aqueous solution containing adenosine 5'-triphosphate was applied and then irradiated with ultraviolet light. The bag glowed pale red.

Comparative Example An acrylic fiber was immersed in a urethane resin dispersion and dried. The acrylic fiber was woven and sewn together with the non-immersed acrylic fiber to produce a forged bag. An aqueous solution containing red luminescent mutant firefly luciferase and adenosine 5'-triphosphate was applied to the surface of the bag, and then irradiated with ultraviolet light. However, the bag did not emit light.

From the above, it can be easily understood that a genuine product to which the marker agent is attached emits light by the counterfeit detection agent and is a genuine product, while a counterfeit product to which the marker agent is not attached is coated with the counterfeit detection agent. Does not emit light, and the product is easily recognized as being fake.

[0023]

According to the present invention, the authenticity of a product can be easily discriminated even by ordinary consumers, so that damage caused by a fake can be prevented beforehand, and counterfeit / counterfeit products can be put on the market. It can prevent distribution and maintain distribution order.

Claims (11)

[Claims] 1. A marker agent comprising, as an active ingredient, at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof. 2. The marker agent according to claim 1, wherein the active ingredient is luciferin, a luciferin derivative, a luciferin precursor, anthracene or an anthracene derivative. 3. A fake detection agent containing a compound capable of reacting with at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof to obtain luminescence or fluorescence. 4. A fake detection agent containing luciferase and ATP. 5. The fake detection agent according to claim 4, further comprising a metal salt. 6. A fake detection agent comprising, as an active ingredient, at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof. 7. A marker agent containing a compound capable of obtaining luminescence or fluorescence by reacting with at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof. 8. A method for producing a genuine product by using (1) attaching a marker agent to a fiber or cloth and using the fiber or cloth, or (2) attaching the marker agent to a metal fitting to be attached to the genuine product. To a product on the market, a fake detection agent containing a compound capable of reacting with the marker agent is applied, and a reaction between the marker agent and the fake detection agent or a reaction derived from the reaction product is detected. A method for determining whether a product on the market is genuine. 9. A marker agent containing, as an active ingredient, at least one luminescent substrate selected from a chemiluminescent substrate, a bioluminescent substrate and a derivative thereof, is attached to a fiber or cloth, and the fiber or cloth is attached to the marker agent. (2) a compound which is attached to a metal fitting to be attached to a genuine product, and which is capable of producing luminescence or fluorescence by reacting with the marker agent on products on the market. A method for determining whether or not a product distributed in the market is genuine by applying a fake detection agent containing, and obtaining fluorescence or luminescence. 10. Shipping and / or inventory management of the marker agent according to claim 1 or 7, shipping and / or inventory management of a fiber, cloth or metal fitting to which the marker agent is attached, and a marker. The fiber, cloth or metal fittings with the agent and marker agent are managed so that they are not used for purposes other than genuine products. A method of applying a contained fake detection agent and obtaining fluorescence or luminescence, thereby discriminating the authenticity of a product and preventing the distribution of a counterfeit product. 11. The marker agent according to claim 1 or 7 and a compound capable of reacting with the marker agent to obtain luminescence or fluorescence are attached to a fiber or cloth, and a genuine product is produced using the fiber or cloth. Manufacturing or (2) attaching the marker agent and a compound capable of reacting with the marker agent and obtaining luminescence or fluorescence to a metal fitting to be attached to a genuine product; Or, a method of determining whether or not a product on the market is genuine based on whether or not fluorescence is obtained.