JP2002119222A - Additive for artificial feed for silkworm, additive for livestock feed and feed - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an additive for feeds comprising a substance characterized in that the substance has an absorption spectrum thereof substantially only at 241 nm in the ultraviolet part or further has the maximum value substantially at 277.5 nm without the maximum value in the visible part in which the substance having the maximum value only at 241 nm is available from an ang-khak (a raw material) and thereby the substance having the maximum value substantially even at 277.5 nm has a benzene ring or a cyclohexane ring in the chemical structure as an active ingredient or a feed comprising the substance. SOLUTION: A polyethylene glycol-mono-p-alkylphenyl ether, its reduced substance (common name: a polyethylene glycol-mono-p-alkylcyclohexyl ether), ethylene glycol-mono-phenyl ether or an alkylphenol is used as an active ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a compound characterized by having a specific skeleton in its specific absorption spectrum and its structural formula (hereinafter referred to as an active substance).
And a feed containing the active substance.

[0002]

2. Description of the Related Art It is well known that koji such as rice koji and barley koji is used for producing miso, soy sauce, sake and the like. Also,
Recently, koji containing Benikoji has been attracting attention as a health food material. It is also known that chlorella, alfalfa, kelp, and wakame are good for health. further,
Mulberry leaves have long been used as feed for silkworms. However, from these, polyethylene glycol-mono-p-isooctyl phenyl ether [Triton (trade name, hereinafter the same) X-
100], and this Triton X-100
It has not been known that the active substance of the present invention containing is effective for rearing silkworms and livestock. Also, koji is said to be unsuitable for the growth of silkworms.

[0003]

DISCLOSURE OF THE INVENTION The present inventors have developed koji, chlorella, spirulina, alfalfa, mulberry leaves, tea leaves,
Chlorophyll and / or pheophytin-containing plant material such as rice straw, wheat straw, spinach, seaweed, kelp, etc.
These are sometimes collectively referred to as koji, etc.). The possibilities and their active ingredients were discussed.

[0004]

[Procedure to Complete the Invention] The present inventor has invented several methods for producing a substance that can be used as an artificial feed additive for silkworms from a plant containing chlorophyll and / or pheophytin so far (for example, see Japanese Patent Application Laid-Open Publication No. H11-163873). Kaihei 6-197704
Nos. 8-103228, 8-126577, and 9-71587). All of these are basically
Extract the ground product of chlorophyll and / or pheophytin-containing plant with a hydrophilic organic solvent such as methanol, and mix the extract with an activator such as vitamin C, phenolic acid, phenolaldehyde, amino acid or an aqueous solution of a water-soluble nickel salt. The mixture is mixed to make the concentration of the hydrophilic organic solvent about 70 to 80%, and then extracted with a non-hydrophilic organic solvent such as hexane, the solvent is removed from the extract, and the residue is purified.

[0005] It was discovered that powder of seaweed (containing pheophytin) such as kelp and wakame, or a methanol extract thereof was effective for the growth of silkworms, and that biosynthesis of proteins was performed similarly to other animals other than silkworms. Has also been studied, and the research target has been able to proceed with the pursuit of animal growth promoting substances without being bound by mulberry components [Masami Kamata, Shinpei Ito: Food Processing Technology Vol. 3 No.2, pp66 (1983)], and further studies on seaweed extracts showed that the food hormones indoleacetic acid and gibberellin had an effect of promoting the growth of silkworms. 1 x per animal
We found a completely new finding that cocoon weight and cocoon layer weight could be increased by percutaneous absorption of a trace amount of 10 ^-6 mg, and as a result, the growth-promoting effect of silkworm was not limited to mulberry leaf components. It became clear that it was also important to expand the scope [Masami Kamada, Shinpei Ito: Agriculturalization
Vol.58 No.8 pp779 (1984)].

[0006] In pursuit of the seaweed components, the extraction with a 70% methanol-water mixture was effective for rearing silkworms. Therefore, extraction with a hydrophilic organic solvent such as methanol was examined. Extracting mulberry leaf powder with methanol gives a chlorophyll blue-green liquid. When the concentration of methanol is gradually reduced by adding water to the liquid, the color gradually changes, and when the concentration falls below 60%, the color becomes yellow. Before discoloration, a non-hydrophilic organic solvent such as hexane was added to the solution portion with a methanol concentration of 80 to 70%, extracted, and the solvent was removed.
When this was added to the diet containing no mulberry leaf powder and fed to the silkworm, the silkworm ate this and grew weakly. It has been found that chlorophyll produces a substance having an ingestive effect.

On the other hand, benzene is added to the above-mentioned solution having a methanol concentration of 80 to 70%, and when left to stand, glittering crystals are formed, which are found to be Mg crystals, and Mg is removed from chlorophyll. Pheophytin-related substances are produced by hydrolyzing pheophytin,
It has also become clear that the hexane extract of the hydrolyzed product has a feeding effect on silkworms.

Based on the above findings, it is considered that when the methanol concentration of the above-mentioned methanol extract is reduced to 80 to 70%, a component having a desired eating effect is produced, and a catalytic component for promoting the production is produced. The search for a substance and various attempts resulted in the invention described above.

[0009]

Means for Solving the Problems and Embodiments of the Invention However, it has been found that these methods are not always easy to produce components having a feeding effect and to separate and purify them. Therefore, we returned to the starting point and decided to re-examine what kind of mulberry leaf powder-added artificial feed is considered to be practical. The mulberry leaf powder-added feed is composed of the following components. Defatted soybean powder, starch, okara,
Cellulose, β-sitosterol, vitamin C, vitamins other than vitamin C, citric acid, and mulberry leaf powder. Then, the powder mixture is kneaded by adding water, and then sterilized to a feed. No special components are used, and no special operation is performed.

Conventionally, it has been considered that feed has a feeding effect because mulberry leaf powder, which is one of feed materials, contains a special active ingredient. No special ingredients have been found, and the above feed ingredients have not been found to have particularly high biological activity. It was thought that a component having a food effect (hereinafter sometimes referred to as an active component) was produced, and that it promoted the feeding and growth of the silkworm.

As a result of the examination, 1) the addition of citric acid and vitamin C, which are not considered to be particularly effective in ordinary feed, is considered to be indispensable. 2) When the silkworm becomes a mature silkworm in which fibroin synthesis becomes active, the pH of the body fluid increases, and the silkworm becomes strongly alkaline. Therefore, the presence of an alkaline substance may be essential. 3) Mulberry leaf powder is known to have a large amount of calcium salt as an alkaline substance. Thought that vitamin C, citric acid, calcium or alkali would be the basis for the formation of the active ingredient.

Therefore, vitamin CNa is used as vitamin C.
Salt, citric acid K salt as citric acid, calcium hydroxide as calcium, potassium hydroxide or dibasic potassium phosphate as alkali, and these were used alone or in various combinations in feed ingredients to examine the effects. .
In addition, as a feed material, in order to avoid the influence of mulberry leaves, alfalfa powder is used as a chlorophyll material, and further, known silkworm feed material components such as soybean powder, starch, cellulose, vitamins other than vitamin C, and sterol are used. Then, the above-mentioned components such as vitamin C and water were added thereto, kneaded to prepare feeds, and a silkworm rearing test was performed for each feed. As a result, it became clear that the combination of (a) calcium hydroxide and vitamin C sodium salt and (b) the combination of dibasic potassium phosphate and potassium citrate exhibited the same breeding effect as when mulberry leaf powder was used. Was.

Based on these findings, chlorophyll and / or
Alternatively, it is necessary that water-soluble calcium and vitamin C or citric acid be present and alkalinized when mixing the aqueous solution of a fine substance of pheophytin-containing plant material extracted with a hydrophilic organic solvent such as methanol. It was revealed that.

Further, a surfactant such as Triton X-100 and / or isopropyl alcohol, polyethylene glycol, propylene glycol, tripropylene glycol, Tris-acetate-EDTA-buffer, Tris-borate-EDTA-buffer and 1 mol 1-Tris-
When an HCL-buffer (pH 8.0) (hereinafter collectively referred to as a surfactant) is present, the solution is made weakly alkaline, and extracted with chloroform. It was found that separation and removal of impurities such as substances were easier, and the degree of purification was good. Therefore, this treatment method was applied to a case where koji such as rice koji and wheat koji were used as a raw material.

As a result, the methanol extract of the koji grind was added to (1) Triton X-100, an alkaline aqueous solution containing isopropyl alcohol, a water-soluble calcium salt and vitamin C, or (2) Triton X-100. , An alkaline aqueous solution containing citric acid and a water-soluble potassium salt are mixed and stirred to a pH of 7 to 10 and a methanol concentration of 65 to 80% (hereinafter, these treatments may be referred to as an activation treatment), and then chloroform is added. Upon extraction, separation from impurities was very easy and a white product was obtained by distilling off chloroform.

Based on this finding, in the activation treatment of the present invention, an alkaline aqueous solution is added to an extract using a hydrophilic organic solvent such as koji, and the organic solvent concentration of the extract is adjusted to 65-80%, pH
In the case of 7 to 10, a surfactant or a surfactant is further added thereto, and (a) an alkaline substance and citric acid are present or (b) a water-soluble calcium salt, vitamin C and an alkaline substance are present. . Hereinafter, the activation process will be described in more detail.

Examples of the alkaline substance include alkali metal salts such as potassium hydroxide, potassium phosphate, sodium hydroxide, sodium carbonate, sodium phosphate and sodium hydrogen carbonate; and organic compounds such as potassium sorbate, sodium sorbate and potassium citrate. Acid alkali salts and the like.

The vitamin C may be free or its salts such as potassium salts and sodium salts. The citric acid may be a free one or a salt thereof such as a potassium salt, a sodium salt and a calcium salt. A potassium salt or a sodium salt may be used as an alkaline substance, and in the case of a calcium salt, may be a water-soluble calcium salt in the above operation (b).

As the surfactant, for example, Triton X-10
0, Triton-114, Triton X-305, Triton X-405,
Triton CF-10, Triton N-101, Triton WR-1339,
Polyethylene glycol type such as polyethylene glycol monomethyl ether, polyethylene glycol mono-p-nonylphenyl ether, polyethylene lauryl alcohol ether, polyethylene sorbitan monolaurate, polyethylene sorbitan monooleate, polyethylene sorbitan monopalmitate, polyethylene sorbitan monosterate, polyethylene Nonionic surfactants such as polyethylene type such as sorbitan trioleate, polyoxyethylene hardened oil, sucrose fatty acid ester, monofatty acid sorbitan, polysorbate and glyceryl monostearate are included.

As the surface-active solvent, isopropyl alcohol, propylene glycol, polypropylene glycol, tripropylene glycol, tris-acetate-EDTA-buffer, tris-borate-EDTA-buffer, 1 mol 1-tris-HCL-buffer (PH 8.0). In the present invention, isopropyl alcohol is advantageous not only as a solvent but also as a substitute for the above-mentioned surfactant.

When the concentration of the hydrophilic organic solvent is 70-80%, its liquidity is pH 7-10, preferably 7.5-9. In the operation (a), one or more of the essential components in (b) may be added by using a surfactant and a surfactant in combination or by using citric acid and a calcium salt in combination. Conversely, in the operation (b), the component (a) may be added with one or more components.

In the operation (a), citric acid is
The amount of the surfactant and the surface active solvent such as isopropyl alcohol may be very small, and the amount of the alkaline substance may be sufficient to maintain the pH of the mixed solution having a hydrophilic organic solvent concentration of 65 to 80%. In the operation (b), an appropriate amount of the water-soluble calcium salt can be used within a range that does not hinder the maintenance of the pH value of the mixed solution.

In the above (a) and (b), the sulfur-containing amino acids such as methionine and cysteine, resorcinol, resorcinic acid, gallic acid, fumaric acid, etc., as described above, which have been described by the present inventor for the silkworms so far In the invention relating to artificial feed, those exemplified as so-called activators may coexist.

The solid content is removed from a mixture of an alkaline hydrophilic organic solvent having a pH of 7 to 10 and a hydrophilic organic solvent concentration of 65 to 80%, followed by extraction with chloroform. The extract is mixed with a water-containing lower alcohol if necessary. Examples of the lower alcohol include methanol and ethanol. The concentration of lower alcohol is preferably about 65 to 85%. This treatment makes it easier to separate and remove the colored layer. The colorless to yellowish-white chloroform layer in the extract is separated. The aliquot is subjected to separation and purification means such as column chromatography, if necessary, concentrated and the solvent is distilled off. Thus, the desired active substance is obtained.

The target active substance is colorless or white-yellow, and its absorption spectrum is substantially in the ultraviolet range of 241 and 27.
It was found that it was characterized by having an extremely sharp maximum at 7.5 nm and no maximum in the visible part.
The term “substantially” means within approximately ± 1 nm.

When the active substance is added to and blended with artificial feed for silkworms and feed for livestock, the growth of the target breeding animal is good,
For example, in the case of silkworms, the weight of cocoons increases, and in the case of dairy cows, the amount of milk produced is increased, and in chickens, pigs, and cows, the effect of producing good results is exhibited.

In the above method, a chlorophyll and / or pheophytin-containing plant material grind containing seaweeds such as koji, spirulina, chlorella and kelp, wakame, tengusa, hondawara, kajime is used as a raw material, and Triton X- is used as a surfactant. The white product obtained using 100 was initially considered a new substance, but as a result of analysis,
It turned out to be a Triton X-100. However, considering the production amount and the amount of Triton X-100 used, it is certain that all of the products are not derived from Triton X-100 used for the activation treatment. When gallic acid was used in place of Triton X-100 in the activation treatment, similar results were obtained. This allows polyethylene glycol-in these materials.
It was found that mono-p-isooctylphenyl ether or its source was present.

Further, as those whose absorption spectrum and chemical structure are similar to Triton X-100 (ethylene polymerization degree n = 7 to 8), Triton X-405 (n = 40), Triton X-405
X-100 Reductant (commonly known as polyethylene glycol mono-
When p-isooctylcyclohexyl ether) and p-isooctylphenol were examined for their activity, the same results as those obtained with Triton X-100 were obtained. By the way, each absorption spectrum is 277 for Triton X-405.
5nm and 240.0nm (hereinafter abbreviated to nm), Triton X-100 reductant 278.0 and 239.5, p-isooctylphenol
It has specific maxima at 278.0 and 240.0.

From the above, polyethylene glycol-mono-p-alkylphenyl ether, polyethylene glycol-mono-p-alkylcyclohexyl ether, ethyleneglycol-monophenyl ether and alkylphenol can have the same activity. The active substance (including the product) exhibits excellent effects on animals including aquaculture insects such as mammals, birds, fish, silkworms and the like, for example, promoting growth, maintaining health, and fortifying nutrients. . This is supported by the results of experiments with various animals such as rats, crucian carp, and silkworms.

As is evident from the above description, the present invention provides: (1) an absorption spectrum whose ultraviolet spectrum is substantially 241 nm;
And an active substance characterized by having an extremely sharp maximum at 277.5 nm but no maximum in the visible region, and having a chemical structure characterized by having a benzene ring or a cyclohexene ring in the formula. Additive for artificial feed. (2) Its absorption spectrum is substantially 241 nm in the ultraviolet.
Animal having an active substance characterized by having an extremely sharp maximum at 277.5 nm but not having a maximum in the visible region, and having a chemical structure having a benzene ring or a cyclohexane ring in the formula. Feed additives. And (3) its absorption spectrum is substantially 241 nm in the ultraviolet.
And an active substance characterized by having a very sharp maximum at 277.5 nm but no maximum in the visible region, and whose chemical structure is characterized by having a benzene or cyclohexane ring in the formula. feed. It is also about. And the active ingredient may be derived from chlorophyll and / or pheophytin-containing plant material such as koji, chlorella, spirulina, seaweeds, mulberry leaves, tea leaves, horen grass, rice straw, wheat straw,
Commercially available polyethylene glycol-mono-p-alkylphenyl ether, its reduced form, ethylene glycol-monophenyl ether or alkylphenol may be used.

Among the uses of the active substance of the present invention, additives for artificial feed for silkworms are described. For example, the substance includes various vitamins such as vitamin B ₆ , nicotinamide, calcium pantothenate, sodium ascorbate, and β. -A blend of nutrients such as sitosterol and inositol, antibacterial substances such as chloromycetin, and fungicides such as oxyquinoline. The degree of incorporation of the active substance into the additive can be determined as appropriate, for example, 10 to 50 mg% by weight.
Degree is sufficient.

Examples of the artificial feed material for silkworms include those prepared by blending known feed materials for silkworms, such as starch, defatted soybean powder, agar, and cellulose powder, but are not limited thereto. The addition ratio to the feed material may be about 5 to 40 mg% by weight.

The silkworm artificial feed formed into a plate of an appropriate shape by adding an appropriate amount of water to the artificial feed raw material is stable and exhibits excellent feeding-inducing and growth-promoting effects on the silkworm.

[0034]

Example 1 Calcium hydroxide 2.0 g, Vitamin C-Na
2.0 g and 1.0 g of vitamin C were dissolved in 200 ml of water, followed by filtration to obtain 150 ml of a filtrate (activating solution, pH 10.8). Separately, 400 ml of methanol was added to 60 g of red monkey powder, and the mixture was stirred with a stirrer to perform extraction, filtered, and extracted (pH 9.0).
) Got. After mixing 150 ml of the activating solution and 350 ml of the extract, 1.0 ml of Triton X-100 and isopropyl alcohol
2.0 ml was added and mixed, and stirred and mixed well with a stirrer. After mixing for 5 hours, the yellow-red precipitate was removed by filtration, extracted well with chloroform, and the extract was washed three times with 100 ml of 50% aqueous methanol, and the chloroform layer was separated and separated from the chloroform solution. Removal of the solvent gave an almost colorless oil. Absorption maximum values 277.5 nm and 241.0 nm (measurement solvent: chloroform, the same applies hereinafter). Determined to be the same as polyethylene glycol-mono-p-isooctylphenyl ether (ethylene polymerization degree n = 7 to 8) by NMR measurement. Wheat koji and soybean koji were treated in the same manner as described above to obtain similar ones.

[0035]

Example 2 Methanol was added to 10 g of Aspergillus niger and filtered to obtain 210 ml of a methanol extract. Separately K
3.0 g of ₂ HPO ₄ , 2.0 g of tripotassium citrate, 2.0 ml of isopropyl alcohol and 0.5 ml of Triton X-100 were dissolved in water to make a total volume of 90 ml. After mixing 210 ml of the above extract and 90 ml of the aqueous solution, stir with a stirrer for 2 hours.
Mix (pH of the mixture 8.7). Chloroform was added to this solution, and yellowish red was transferred to the chloroform layer by shaking.The yellow-red chloroform solution was directly subjected to reversed-phase column chromatography, and as it flowed, it was separated into orange and red fractions. The product obtained by distillation was the same as that obtained from rice koji, and the product was physiologically active as it was. However, it was washed with 50% aqueous methanol and extracted with chloroform in the same manner as described in Example 1. By performing the purification treatment, the same active substance as the product of Example 1 was obtained.

[0036]

Example 3 50 g of alfalfa powder and 500 g of methanol
After adding ml, the mixture was extracted and filtered to obtain a filtrate (pH 7.6). Separately, 29 g of potassium diphosphate, 15 g of tripotassium citrate, 0.4 ml of Triton X-100 and 2.0 ml of isopropyl alcohol were dissolved by adding 150 ml of water (pH 9.1), and 350 ml of the above-mentioned filtrate was added thereto. Stir and mix well. After 3 hours, the solution turned dark and dark red (pH 9.1), which was extracted with chloroform, the extract was washed with 50% aqueous methanol, and the solvent was removed to obtain an almost colorless oil. Absorption maximum 277.5nm and 241.0nm

[0037]

Example 4 Extraction was carried out by adding 500 ml of methanol to 30 g of mulberry leaf powder, and the filtrate was collected by filtration. To 350 ml of the filtrate, 1.0 ml of Triton X-100 and 2.0 ml of isopropyl alcohol were added (Solution A). Separately, 3 g of calcium hydroxide, 3 g of vitamin C-Na and 1.5 g of vitamin C were dissolved in 400 ml of water, and 150 ml of the filtrate was added to solution A, followed by stirring and mixing with a stirrer. After 3 hours, the yellow substance was well separated, extracted with chloroform, and the solvent was removed from the extract to obtain an almost colorless oily substance. This was repeatedly purified by washing with 50% aqueous methanol. The absorption maximum of the obtained substance is 277.
5 nm and 241.0 nm.

[0038]

Example 5 10 g of Tororo Kombu (Kokuraya, Hirakata City) was extracted with 500 ml of methanol and filtered to obtain an extract (pH 9.1). Separately, 30 g of potassium diphosphate, 15 g of potassium tertiary citrate, 0.4 ml of Triton X-100 and 2.0 ml of isopropyl alcohol were dissolved in water to make a total volume of 150 ml (pH 8.0). The whole amount was added to the extract, and the mixture was stirred and mixed with a stirrer for 4 hours. The solution disappears yellow and becomes almost colorless (pH 9.0). Chloroform was added to this and extracted,
After chloroform was removed from the extract, washing was repeated with 50% aqueous methanol to purify the extract. The maximum absorption values of the obtained substance were 277.5 nm and 241.0 nm.

[0039]

Example 6 5 g of Chlorella bulk powder was dissolved in 250 ml of methanol, and filtered to remove insolubles. 140 ml of the filtrate was taken, and 24 g of potassium diphosphate, 8 g of potassium citrate, 8 g of Triton X A solution prepared by dissolving 0.4 ml of -100 and 2.0 ml of isopropyl alcohol in water to 60 ml was added, and the mixture was stirred and mixed with a slurler for 3 hours. The liquid turns yellow-green. Chloroform is added to this solution to dissolve the yellow-green portion, and the chloroform solution is directly subjected to reverse-phase column chromatography, and then flown, so that it is separated into a pale yellow and a green fraction, and when further flowing, the pale yellow becomes almost colorless. So, after taking this part and removing the solvent from it,
Washing with% methanol water was repeated, then dissolved in chloroform, and purified by reverse phase column chromatography again. Absorption maxima of product substances 277.5 nm and 241.0 nm

[0040]

[Experimental Example 1] 300 mg of each of the active substances obtained from Koji Koji, Beni Koji, Alfalfa, Mulberry leaf, Tororo kelp and Chlorella obtained in the examples were dissolved in 10 ml of methanol, added to 10.0 g of potato starch, stirred and mixed. Thereafter, the mixture was dried under reduced pressure to obtain a triturated powder, and used in addition to the feed raw material powder.
The feed material composition is as follows. Defatted soybean 43.0 g Potato starch 14.5 g Cellulose 11.0 g Okara 5.2 g Vitamin concentrated powder 9.4 g Crotonic acid 0.4 g Choline chloride 2.0 g β-sitosterol 1.0 g Trituration 10.0 g Vitamin C 1. 5 g citric acid 2.0 g total amount 100 g (30 g of active substance is contained in 10.0 g of each feed material) 2.2 g of water is added to 10 g of feed material and kneaded,
The kneaded material was heat-sterilized in an autoclave to obtain an artificial feed.

(Note 1) The composition of the vitamin powder is as shown in Table 1. As a control, 10.0 g of potato starch was used instead of the double powder.

The artificial feed was placed in a sterile breeding container, the ant silkworms were swept up, and germ-free breeding was performed in a thermostat (25 ° C.). The breeding results are as follows. Table 2 Active substances Number of slaughter heads Third day silkworm body weight on day 9 (mg) Experimental group 1. 1. Obtained from rice koji 226 70.8 Benikou 〃 189 66.9 3. Alfalfa 218 72.3 4. Kuwaha １９ 197 69.3 5. Tororo kelp ２１２ 212 71.6 6. Chlorella ２０８208 69.5 Control group No addition 192 23.7 From these results, it was proved that the active substance obtained from a raw material other than rice koji also had a superior physiological effect as compared with the control.

[0043]

EXPERIMENTAL EXAMPLE 2 A reduced form of triton X-100 (commonly referred to as polyethylene glycol-mono-p-isooctylcyclohexyl), which is considered to be the same substance as polyethylene glycol-mono-p-isooctylphenyl ether (hereinafter referred to as octyl ether). About ether, cycloether), ethylene glycol-mono-phenyl ether (hereinafter referred to as monophenyl) and isooctylphenol, silkworms were reared by using the same composition as in Experimental Example 1 and operating in the same manner. The results were as follows. Table 3 Active substances Number of slaughter heads Day 9 3rd instar silkworm body weight (mg) Cycloether 186 73.7 2. Monophenyl 207 67.9 3. Isooctylphenol 169 69.0 As is clear from the table, all have bioactivity.

[0044]

[Experimental Example 3] Fifth-age stage silkworm rearing test The composition of feed materials used is as follows. Table 4 Control test section Skim soybean 43.0 parts 43.0 parts Cellulose 11.6 11.6 Potato starch 12.5 12.5 Okara powder 28.2 18.2 Crotonic acid 0.2 0.2 β-sitosterol 1.0 1.0 Mulberry leaf powder 10.0 --- Trituration (containing octyl ether) --- 10.0 Vitamin C 1.5 1.5 Citric acid 2.0 2.0 100.0 100.0

After mixing the feed raw material powder, a 2.2-fold amount of water was added, kneaded, spread thinly between two films, and then sterilized in an autoclave for 30 minutes. After sterilization, the film on the upper surface was peeled off, 5th-instar silkworms were placed on the feed, left in a sterile room at 25 ° C., and raised until they became silkworms, and cocoons were formed. The results were as follows, and it was clarified that a cocoon superior to the control using mulberry leaf powder was produced. Table 5 Cocoon performance Mulberry leaf powder use area Octyl ether use area Single cocoon weight 1.76 g 1.82 g Cocoon layer weight 342 mg 356 mg Total thread length 854 m 872 m

[0046]

Industrial Applicability According to the present invention, the usefulness of compounds known only as surfactants and the like as feed additives and nutrients for livestock, poultry, fish for artificial rearing, etc. The present invention provides a simple and inexpensive new production method, and provides an excellent alternative to mulberry leaf regardless of time or region, even if this is limited to application to silkworm artificial feed raw materials. The economic effect is extremely large.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) A23K 1/175 A23K 1/175 F-term (Reference) 2B005 FA03 FA06 FA07 FA08 FA09 FA10 MA05 MB02 MB05 MB07 2B150 AA09 AB02 AB05 AB06 AC24 AD04 AE12 AE33 AE34 AE41 AE48 BC02 BC03 BC04 BC05 CA20 DA41 DA63 DB04 DD12 DD44 DD48 DD49 DD50 DD57 DE13 DH03 DH04

Claims (9)

[Claims] 1. The method according to claim 1, wherein said absorption spectrum is substantially in the ultraviolet.
The active ingredient has extremely sharp maxima at 241 nm and 277.5 nm but no maxima in the visible region, and its chemical structure is characterized by having a benzene or cyclohexane ring in the formula. Additive for silkworm artificial feed. 2. The method according to claim 1, wherein said absorption spectrum is substantially ultraviolet.
An active substance that has extremely sharp maxima at 241 nm and 277.5 nm but no maxima in the visible region, and whose chemical structure is characterized by having a benzene ring or a cyclohexane ring in the formula Additives for animal feed. 3. The method according to claim 1, wherein said absorption spectrum is substantially ultraviolet.
Contains active substances characterized by having extremely sharp maxima at 241 nm and 277.5 nm but no maxima in the visible region, and whose chemical structure is characterized by having a benzene or cyclohexane ring in the formula feed. 4. The method as claimed in claim 1, wherein the active substance is a methanol extract of koji, spirulina, chlorella, or a grind of chlorophyll and / or pheophytin-containing plant material. (A) Triton X-100, isopropyl alcohol, water-soluble calcium salt , An aqueous alkaline solution containing vitamin C or an alkaline aqueous solution containing (b) Triton x-100, isopropyl alcohol, and citric acid are stirred and mixed to a pH of 7 to 10 and a methanol concentration of 65 to 80%, and then extracted with chloroform. The additive according to claim 1 or 2 or the feed according to claim 3, which comprises a food that can be obtained by the above method. 5. The method according to claim 5, wherein the koji is Benikoji, Koji,
The additive or feed according to claim 4, which is wheat koji or soy koji. 6. The additive or feed according to claim 4, wherein the chlorophyll and / or phephytin-containing plant material is mulberry leaf, tea leaf, spinach, rice straw, wheat straw or seaweed. 7. The artificial silkworm of claim 1, wherein the active substance is polyethylene glycol-mono-p-alkylphenyl ether, a reduced form thereof, ethylene glycol-monophenyl ether or alkylphenol. Additives. 8. The livestock feed additive of claim 2, wherein said active substance is polyethylene glycol-mono-p-alkylphenyl ether, its reduced form, ethylene glycol-monophenyl ether or alkylphenol. 9. The feed according to claim 3, wherein said active substance is polyethylene glycol-mono-p-alkylphenyl ether, its reduced form, ethylene glycol-monophenyl ether or alkylphenol.