JP2002098687A - Inspection agent for dermatropic pathogen - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inspection agent which surely catches a dermatropic pathogen including the human Sarcoptes scabiei that inhabits on the surface of the human skin including pores, etc. SOLUTION: This inspection agent for dermatropic pathogen contains a water-soluble macromolecule. The macromolecule is prepared by utilizing, for example, a homopolymer or copolymer having an ionic functional group, polyvinyl alcohol, polyvinyl pyrrolidone, vinyl acetate-vinyl pyrrolidone copolymer, polyethylene oxide, polyvinyl methyl ether, a hydrolysate of collagen or its modified product, pectin, β-1,3-glucan, α-glucan, (hemi)celluloses, etc. The macromolecule can contain a non-water soluble macromolecule, such as the poly(vinyl acetate), poly(metha)ester acrylate, etc., within the range in which the non-water soluble molecule does not hinder the water solubility of the water-soluble macromolecule. In addition, the macromolecule can also contain a plasticizer, oily component, surface-active agent, filler, heat insulating agent, solvent, preservative, horn dissolving agent, refrigerant, medicine, perfume, antioxidant, etc., as necessary.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a skin parasitic pathogen, and more particularly to a test material, a test material kit and a test method for mites, fleas and lice.

[0002]

2. Description of the Related Art There are many pathogens, such as mites, fleas and lice, which are parasitic on human skin. Among them, scabies are human scabies
(Sarcoptes scabiei var hominis: common name mite) is an infectious disease accompanied by intense itch, and its infection is expanding mainly in geriatric care facilities. Human scabies larvae and male adults live on the surface of human skin, while female adults live in the stratum corneum, dig through holes called scabies tunnels, and move through the skin while laying eggs. In addition, since the infection is transmitted by contact infection, transmission not only between patients but also from a medical staff to their families becomes a problem.

[0003] In foreign countries, treatments using pesticides such as γ-BHC have been successful, but in Japan, their use has not been approved, and the treatment is extremely difficult. Therefore, when infection is suspected, prompt and reliable diagnosis and the immediate isolation of patients are of paramount importance in preventing transmission.

[0004]

The definite diagnosis of scabies is made by checking the worm body (including the husks) or eggs, but compared to a male adult or larva whose location is difficult to identify, it is a scabies tunnel. Female adults lurking in them are relatively easy to find. The current scabies inspection method carefully examines the scabies tunnel, pricks the tip of the tunnel with a needle,
Human scabies were observed under a microscope from the collected samples. However, although relatively easy, where it is unknown, the detection rate is as low as about 60%.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies on test materials for skin parasitic pathogens. As a result, the test material for skin parasitic pathogens containing water-soluble polymers contains pores and the like. The present inventors have found that skin parasitic pathogens such as human scabies living on the skin surface are reliably captured and the detection rate is dramatically improved, and the present invention has been completed.

That is, the present invention includes the following inventions. (1) A test material for skin parasitic pathogens containing a water-soluble polymer. (2) The inspection material according to (1), which has transparency. (3) The inspection material according to (1) or (2), which is in the form of a sheet. (4) The inspection material according to the above (1), which is in the form of a sheet and is formed by laminating a substrate and a layer containing a water-soluble polymer. (5) The inspection material according to (4), wherein the base material has air permeability and flexibility. (6) The inspection material according to (1) or (2), which is in a paste form. (7) A test kit for skin parasitic pathogens, comprising the test material according to (2) or (6) and a transparent adhesive sheet. (8) A method for examining skin parasitic pathogens using a transparent adhesive sheet when the test material according to (2) or (6) is peeled from the skin.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The water-soluble polymer used in the present invention includes, for example, ionic functional groups (for example, carboxyl group, amino group, sulfonic acid residue, phosphoric acid residue, sulfate ester group, phosphate ester). Group or the like) (for example, random, block, alternating) or a salt thereof (for example, poly (meth) acrylic acid, (meth) acrylic acid / (meth) acrylate copolymer, poly ( Alkanolamine (meth) acrylate, (meth) acrylic acid / styrene copolymer or a salt thereof, poly (meth) acryloyloxyethyltrimethylammonium chloride, chondroitin sulfate or a salt thereof), polyvinyl alcohol,
Polyvinylpyrrolidone, vinyl acetate / vinylpyrrolidone copolymer, polyethylene oxide, polyvinyl methyl ether, a hydrolyzate of collagen or a modified product thereof (eg, gelatin), pectin, other β-1,3-glucan, α-glucan, Hemi) celluloses (for example, hydroxypropyl cellulose) and the like.

The test material of the present invention can contain a water-insoluble polymer such as polyvinyl acetate and poly (meth) acrylate as long as the water solubility is not impaired. The test material of the present invention may further contain, if necessary, a plasticizer, an oily component, a surfactant, a filler, a humectant, a solvent, a preservative, a keratolytic agent, a refreshing agent, a drug, a fragrance, An oxidizing agent or the like can be contained.

[0009] Examples of the plasticizer include ethylene glycol, diethylene glycol, triethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, tripropylene glycol, polypropylene glycol, 1, and more.
Examples thereof include polyhydric alcohols such as butanediol such as 3-butanediol and 1,4-butanediol, glycerin, diglycerin, triglycerin, and higher polyglycerin.

[0010] The content of these plasticizers in the test material is preferably about 0.01 to 100 parts by weight, more preferably about 1 to 30 parts by weight, per 100 parts by weight of the water-soluble polymer. If the content of the plasticizer is too small, depending on the average molecular weight and the content of the water-soluble polymer, the test material is cured,
Cracks occur, handling becomes poor, and if the content of the plasticizer is too large, depending on the average molecular weight or the content of the water-soluble polymer, the test material is softened, and the adhesive strength is reduced,
Poor capture of skin parasitic pathogens.

Examples of the oil component include hydrocarbons such as liquid paraffin, squalane, petrolatum, and solid paraffin; natural oils such as jojoba oil, castor oil, olive oil, egg yolk oil, and coconut oil; silicone oils such as polydimethylsiloxane; Examples include higher fatty acids such as oleic acid and isostearic acid, higher alcohols such as lauryl alcohol, and esters such as isopropyl myristate.

The content of these oily components in the test material is 0.01 to 100 per 100 parts by weight of the water-soluble polymer.
About 1 part by weight is preferable, and about 1 to 30 parts by weight is more preferable. If the content of the oily component is too small, the pain at the time of peeling the test material becomes strong, and if the content of the oily component is too large,
The film strength is reduced, and the adhesion to human scabies is reduced due to the oily component oozing out on the surface.

Examples of the surfactant include nonionic surfactants such as glycerin fatty acid ester, sorbitan fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene castor oil, polyoxyethylene hardened castor oil, and polyoxyethylene. Examples thereof include N-acyl amino acid salts which are anionic surfactants such as alkyl ethers, and aliphatic quaternary ammonium salts which are cationic surfactants, and betaines which are amphoteric surfactants.

The content of these surfactants in the test material is 0.01 to 10 per 100 parts by weight of the water-soluble polymer.
It is preferably about 5 parts by weight, more preferably about 0.05 to 5 parts by weight. If the content of the surfactant is too small, it is difficult for the oily component to be uniformly dispersed in the test material, and if the content of the surfactant is too large, the adhesive force to human scabies decreases.

Examples of the filler include inorganic fillers such as talc, sericite, calcium carbonate, kaolin, silicic acid anhydride, hydrous silicic acid, titanium oxide, mica titanium, medicinal charcoal, carbon black, polyamide, polyethylene,
Organic fillers such as powders (especially spherical powders) of cellulose, polymethyl methacrylate and the like, and those obtained by subjecting these to hydrophobic treatment with metallic soap, ester, silicone, or the like.

The content of such filler (particularly pigment) in the test material is 15 per 100 parts by weight of the water-soluble polymer.
0 parts by weight or less is preferable, and about 1 to 75 parts by weight is more preferable. If the content of the filler is too large, the performance of capturing the skin parasitic pathogen decreases. Examples of the solvent include water and lower alcohols such as ethanol and isopropanol, and mixtures thereof.

The solvent content in the paste-like test material is usually 10 to 95.5% by weight, preferably 20 to 80% by weight.
The content of the solvent in the composition constituting the layer containing the water-soluble polymer of the sheet-like inspection material is usually 30% by weight.
Or less, preferably 20% by weight or less. Examples of preservatives include paraoxybenzoic acid esters such as methyl paraoxybenzoate, and benzalkonium chloride. Examples of the humectant include various alcohols and sugars.

Examples of the keratolytic agent include lactic acid, papain, biobrase, salicylic acid, glycolic acid, citric acid and malic acid. Examples of the cooling agent include menthol and camphor. Examples of the drug include an antipruritic, an analgesic, an anti-inflammatory, and a bactericide.
Examples of the antioxidant include ascorbic acid, tocopherol and derivatives thereof. The form of the inspection material of the present invention is not particularly limited, and examples thereof include a sheet shape and a paste shape.

The sheet-like test material is preferably used after being supplied with a solvent at the time of use, and then applied to human skin. Examples of the sheet-like inspection material include those obtained by laminating a base material and a layer containing a water-soluble polymer. The base material has predetermined air permeability and flexibility (flexibility). Examples of such a base material include those made of a porous material. Examples of the porous material include a non-fibrous or fibrous porous material.

Examples of the non-fibrous porous material include a membrane filter, a foam (foamed polypropylene, foamed polyethylene, foamed polyurethane, etc.), various porous films, a mesh and the like. Examples of the fibrous porous material include woven and knitted fabrics, nonwoven fabrics, papers, aggregates of short fibers, and the like. Here, the woven or knitted fabric includes a woven or knitted fabric such as a woven fabric or the like. As the woven fabric, all types of practically usable ones, for example, plain weave, oblique weave, and satin weave can be used. There is also no particular limitation on the knitted structure, for example, weft knitting (flat knitting), warp knitting (tricot knitting), circular knitting, flat knitting,
Knitted knitting and the like can be mentioned. When a nonwoven fabric is used, the packing density (bulk density) of the fibers and the like are not particularly limited. As papers, ordinary paper materials (Western paper, Japanese paper) or a laminate thereof,
Various synthetic papers can be used.

The fibers constituting such a fibrous porous material include, for example, natural fibers such as cellulose fiber, cotton, linter, kabok, flax, hemp, ramie, silk, wool, etc.
Chemical fibers such as nylon (polyamide), tetron, rayon, cupra, acetate, vinylon, acrylic, polyethylene terephthalate (polyester), and polypropylene, or combinations of two or more of these natural and chemical fibers (such as blend spinning) may be mentioned. it can.

The thickness of the substrate is not particularly limited, but is preferably 20 to 1000 μm. It is preferable that such a substrate itself has air permeability. The reason is that when a sheet-like test material described later is adhered to the skin, the solvent (moisture, alcohol content, etc.) passes through the base material and evaporates, and the sheet-like test material is dried uniformly and well. That's why.

The composition constituting the layer containing the water-soluble polymer (hereinafter also referred to as “water-soluble polymer layer”) is given fluidity in the presence of a sufficient solvent such as water or alcohol,
In addition, it is preferable to prepare so that a film can be formed in a dry state. For the lamination of the substrate and the layer containing the water-soluble polymer, for example, a water-soluble polymer coating solution is applied to the release agent-coated surface of the transfer film in which the release agent is applied to the transfer film substrate. It can be carried out by a method of drying, subjecting to a water treatment as required, and superposing the substrate on the water-soluble polymer layer, or a method of applying a water-soluble polymer coating solution on one surface of the substrate and drying. Thereby, a laminate of the substrate and the layer containing the water-soluble polymer is obtained. When the substrate is composed of a porous material as described above, a part of the water-soluble polymer layer may be configured such that the water-soluble polymer is impregnated or embedded in a part of the substrate. Good. As a method of application, any method such as coating using a roll, an air knife, a knife, gravure, a Meyer bar, a fountain die, spray coating, brushing, dipping, etc. may be used. The thickness of the layer containing the water-soluble polymer is preferably from 50 to 400 μm (after drying), and more preferably from 100 to 300 μm (after drying).

Examples of the transfer film include paper substrates such as glassine paper, woodfree paper, coated paper, and cast-coated paper; laminated paper obtained by laminating a thermoplastic resin such as polyethylene on these paper substrates; or polyethylene terephthalate. Polyester films such as polybutylene terephthalate and polyethylene naphthalate, polyolefin films such as polypropylene and polyethylene, polyvinyl chloride films, polyvinylidene chloride films, polyvinyl fluoride films, polymethyl methacrylate films, polycarbonate films, ethylene-vinyl acetate copolymer Examples thereof include those obtained by applying a release agent such as a silicone resin, an alkyd resin, or a fluororesin to a plastic film such as a united film. The thickness of the transfer film is not particularly limited, but is usually about 20 to 150 μm.

After the test material of the present invention is applied to human skin,
After drying to form a film, it is preferable that the film be peeled off and subjected to an inspection using a microscope or the like. As a microscope, a surface microscope for irradiating light to the surface of an observation object and observing it with reflected light, and a light transmission microscope for irradiating light from the back of the object and observing it with transmitted light are commonly used.

In the case of inspection using a surface microscope, the surface of the water-soluble polymer layer (insect-trapping layer) of the inspection material is directly observed in the same manner as a loupe, so that the inspection material may be opaque. Although this is a simple observation method, if the surface has severe irregularities, it may be difficult to observe a concave portion (a convex portion of the skin). On the other hand, when used for inspection with a light transmission microscope, all layers of the water-soluble polymer layer are to be inspected, but since the inspection material needs to transmit light, the inspection material of the present invention is transparent. It is preferable to have

Such an inspection material does not include a base material such as a nonwoven fabric, and further includes, among the components described above, colored components such as fillers such as titanium oxide, talc, silicic anhydride, carbon black, and other water-soluble materials. It can be produced by using as little insoluble components as possible in the conductive polymer layer.

When a substrate such as a non-woven fabric is not provided, the skin adhesive strength is larger than the coating strength of the test material (water-soluble polymer layer). Since it is difficult to perform this, it is preferable that a transparent pressure-sensitive adhesive sheet is stuck on the dried water-soluble polymer layer (insect capturing layer), and the pressure-sensitive adhesive sheet is peeled off from the skin.

That is, the present invention provides a kit for testing a skin parasitic pathogen, comprising the test material according to the above (2) or (6) and a transparent adhesive sheet, and the above (2) or (6) The present invention also provides a method for examining skin parasitic pathogens using a transparent pressure-sensitive adhesive sheet when the test material described in 1) is peeled from the skin.

Examples of the transparent pressure-sensitive adhesive sheet include:
For example, on one surface of a transparent sheet substrate such as polyethylene terephthalate (PET), polyurethane, polyethylene, or polypropylene, an adhesive such as an acrylic adhesive, a urethane-based adhesive, or a silicone-based adhesive is coated with the above-mentioned aqueous solution. What was applied by the same method as the method of applying a hydrophilic polymer coating liquid is mentioned. The thickness of the sheet substrate is
Although there is no particular limitation, it is usually 20 to 100 μm. The thickness of the pressure-sensitive adhesive layer is not particularly limited, but is usually 10 to 50 μm.
It is.

The site to which the test material of the present invention is applied is preferably a site where the target skin parasitic pathogens, for example, mites, fleas and lice, are present at high frequency. Is more preferable. For example, when the target fossa skin parasitic pathogen is a human mange, as a site to which the test material of the present invention is applied, for example, between fingers,
Fingers, palms, flexed arm joints, elbow fossa, anterior folds of the axilla, breast, lower abdomen, penis, scrotum, groin, umbilicus, medial thigh, buttocks, etc. In terms of ease, the palm, the periphery of the umbilicus, the inside of the thigh, and the like with less body hair are preferable.

Examples of the shape of the sheet-like inspection material include a rectangle, a circle, an ellipse, a square, a heart, a diamond, a donut, and the like. Is preferred. Hereinafter, an example of an embodiment of the present invention suitable for inspection with a microscope (in particular, a light transmission microscope) will be described with reference to FIG.

The sheet-like inspection material (A) is composed of a water-soluble polymer layer (insect capturing layer) and release sheets provided on both sides thereof. As the release sheet, the same release sheet as described above is used. Paste inspection material (B)
Is composed of a solution in which the aforementioned transparent component is dissolved in a solvent such as ethanol or water.

In the sheet-like inspection material (A), one of the release sheets is peeled off, the adhesive surface and / or a portion to which the skin is applied is wetted with water or the like, attached to the skin surface, and the other release sheet is removed. Peel off to form a water-soluble polymer layer (insect capture layer) on the skin surface. In the paste-like test material (B), an appropriate amount is applied to the skin surface to form a water-soluble polymer layer (insect capture layer). For both the sheet-like inspection material (A) and the paste-like inspection material (B), after sufficiently drying the water-soluble polymer layer (insect capture layer), the transparent adhesive sheet is attached thereon, The water-soluble polymer layer (insect capture layer) is peeled from the skin together with the adhesive sheet.

Next, the water-soluble polymer layer (insect capture layer) is attached to a slide glass and subjected to microscopic inspection. At this time, after dissolving the water-soluble polymer layer (insect capture layer) in an appropriate solvent, for example, methanol, ethanol, or water, the solution was stuck to a slide glass and subjected to microscopic examination, and the sample became cloudy when peeled. It is preferable because the water-soluble polymer layer dissolves and becomes transparent, thereby facilitating detection of insects. Further, it is more preferable to use a 20% aqueous solution of potassium hydroxide since the attached keratin is dissolved.

[0036]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, which should not be construed as limiting the scope of the present invention. (Example 1) Sheet-like inspection material Water-soluble polymer coating solutions having the compositions shown in Table 1 were prepared.

[0037]

TABLE 1 Vinyl acetate / vinyl pyrrolidone copolymer 100 parts by weight Polyvinyl acetate 15 parts by weight Squalane 3 parts by weight 1,3-butanediol 3 parts by weight Silicic anhydride 24 parts by weight Titanium oxide 1.5 parts by weight Ethanol 75 parts by weight Parts water 37.5 parts by weight

First, a vinyl acetate homopolymer solution and a filler were added to an ethanol solution of vinyl acetate and vinyl pyrrolidone copolymer, and these were stirred. Next, a plasticizer and an oil component were dissolved and dispersed in water, added to the copolymer solution, and sufficiently stirred to obtain a water-soluble polymer coating solution. On the other hand, a nonwoven fabric (thickness: 400 μm) made of polyester fiber was prepared as a substrate.

The coating solution was applied to the surface of a transfer film in which a release agent made of a silicone resin was applied to one surface of a 50 μm polyethylene terephthalate film, and dried to form a water-soluble polymer layer having a dry film thickness of 150 μm. Formed. Next, the water-soluble polymer layer is subjected to water treatment by steam damping to be softened and softened. Thereafter, the base material and the water-soluble polymer layer are laminated, and these are pressed by a roller, and a part of the softened water-soluble polymer is partially removed. Was impregnated and embedded in a substrate. The thickness of the overlapping portion between the substrate and the water-soluble polymer layer was about 20% of the thickness of the substrate. As described above, the sheet-like inspection material of the present invention was prepared. The solvent content of the water-soluble polymer layer after drying was 5% by weight.

(Example 2) Paste test material The same composition as the water-soluble polymer coating solution of Example 1 was mixed to obtain a paste test material. The content of the solvent (water and ethanol) was 43.4% by weight.

Example 3 A sheet-like inspection material having transparency A water-soluble polymer coating solution was prepared in the same manner as in Example 1 except that silicic anhydride and titanium oxide were not blended. The coating liquid was applied to the surface of a release sheet made of polyethylene terephthalate having a release agent made of a silicone resin applied to the surface thereof, and dried to form a water-soluble polymer layer having a dry film thickness of 180 μm. Further, a release sheet similar to the above release sheet is laminated on the surface of the water-soluble polymer layer, and a transparent sheet-like inspection material comprising a water-soluble polymer layer in which release sheets are laminated on both sides is prepared. did. The solvent content of the water-soluble polymer layer after drying was 5% by weight. On the other hand, a transparent polyethylene terephthalate film (50 μm thick)
m) was coated with an acrylic pressure-sensitive adhesive (thickness: 15 μm) to prepare a transparent pressure-sensitive adhesive sheet.

Example 4 Transparent Paste Inspection Material The same compositions as those of the water-soluble polymer coating solution of Example 1 were mixed except that silicic anhydride and titanium oxide were not added, and the transparency was measured. A paste-like inspection material was obtained. The content of the solvent (water and ethanol) was 48.2% by weight. On the other hand, a transparent pressure-sensitive adhesive sheet was prepared in the same manner as in Example 3.

(Test Examples) The following tests were conducted for each of five men and women. (Sheet-like inspection material of Example 1) (1) One drop of black ink was applied to the back of a well-washed hand, uniformly spread and dried. (2) The surface of the water-soluble polymer layer (insect body capturing layer) of the sheet-like inspection material of Example 1 was wetted with water and affixed to a portion where ink was applied. (3) After about 15 minutes, the dried sheet-like inspection material was peeled off. (4) The amount of black ink remaining on the skin was observed. (5) Black ink adhering to the surface of the water-soluble polymer layer (worm capturing layer) of the peeled sheet-like inspection material was observed with a surface microscope (50 ×).

(Paste-like inspection material of Example 2) (1) One drop of black ink was applied to the back of a well-washed hand, uniformly spread, and dried. (2) The surface of the paste-like inspection material of Example 2 adhered to the skin was applied to the portion where the ink was applied so as to be thin and uniform. (3) After about 20 minutes, the dried and film-formed paste-like inspection material was peeled off. (4) The amount of black ink remaining on the skin was observed. (5) The black ink adhering to the surface of the peeled film-like paste-like inspection material adhered to the skin was observed with a surface microscope (50 ×).

(Transparent sheet-like inspection material of Example 3) (1) One drop of black ink was applied to the back of a well-washed hand, uniformly spread, and dried. (2) Peel off one release sheet of the transparent sheet-like inspection material of Example 3, wet the surface of the exposed water-soluble polymer layer (insect capture layer) with water, and apply it to the portion where ink was applied. Then, the other release sheet was peeled off. (3) After about 15 minutes, the transparent pressure-sensitive adhesive sheet of Example 3 was attached to the surface of the dried sheet-like inspection material, and then the sheet-like inspection material and the pressure-sensitive adhesive sheet were peeled off from the skin. (4) The amount of black ink remaining on the skin was observed. (5) The peeled sheet-like inspection material was wetted with water and attached to a slide glass, and then the black ink adhered from the adhesive sheet side was observed with a light transmission microscope (50 times).

(Transparent paste-like inspection material of Example 4) (1) One drop of black ink was applied to the back of a well-washed hand, uniformly spread and dried. (2) The surface of the paste-like test material of Example 4 adhered to the skin was thinly and uniformly applied to the portion where the ink was applied. (3) After about 20 minutes, the transparent pressure-sensitive adhesive sheet of Example 4 was attached to the surface of the dried and film-formed paste-like test material, and the film-formed paste-like test material was removed from the skin together with the pressure-sensitive adhesive sheet. Peeled off. (4) The amount of black ink remaining on the skin was observed. (5) The peeled paste-like test material was wetted with water and attached to a slide glass, and then the black ink adhered from the adhesive sheet side was observed with a light transmission microscope (50 times).

(Comparative Example: General adhesive sheet) The sheet-like inspection material of Example 1 except that instead of the sheet-like inspection material, a general adhesive sheet in which an acrylic adhesive was spread and applied on a 25 μm stretched polypropylene film was applied. A test was performed in the same procedure as described above.

(1) Observation results of the amount of black ink remaining on the skin Evaluation criteria are as follows. 1 Almost not removed 2 Surface is removed but nothing in pores / skins is removed 3 Some in pores / skins is also removed 4 Almost removed 5 Completely removed Table 2 shows the results.

[0049]

[Table 2]

As is clear from Table 2, all of the test materials of Examples 1 to 4 were able to remove black ink in pores and skin grooves as compared with the general adhesive sheet of Comparative Example. In addition, there was no difference between men and women in how to take.

(2) Results of Observation of Black Using a Surface Microscope In both Examples 1 and 2, black was adhered to the surface of the test material.
It was confirmed that black ink particles were captured from the skin. (3) Observation of black ink by light transmission microscope In both Examples 3 and 4, black particles (aggregates) could be clearly confirmed without being affected by skin keratin and the like.

[0052]

According to the test material of the present invention, skin parasitic pathogens such as human scabies living on the skin surface including pores and the like can be reliably captured, and the detection rate can be dramatically improved. .

[Brief description of the drawings]

FIG. 1 is a diagram for explaining an example of an embodiment of the present invention suitable for inspection with a microscope.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) G01N 33/50 G01N 33/50 Q 33/569 33/569 A

Claims (8)

[Claims] 1. A test material for skin parasitic pathogens containing a water-soluble polymer. 2. The inspection material according to claim 1, which has transparency. 3. The inspection material according to claim 1, which is in the form of a sheet. 4. The inspection material according to claim 1, which is in the form of a sheet and is formed by laminating a substrate and a layer containing a water-soluble polymer. 5. The inspection material according to claim 4, wherein the substrate has air permeability and flexibility. 6. The inspection material according to claim 1, which is in the form of a paste. 7. A test material kit for skin parasitic pathogens, comprising the test material according to claim 2 and a transparent adhesive sheet. 8. A method for examining skin parasitic pathogens using a transparent adhesive sheet when peeling the test material according to claim 2 or 6 from the skin.