JP2002028691A - Decolorant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To rapidly decompose/decolorize indanthrene dyes, disperse dyes or reactive dyes satisfactorily. SOLUTION: The dyes selected from indanthrene dyes, disperse dyes and reactive dyes are decolorized by incorporating one or more microbes selected from genus Klebsiella, genus Flavobacterium and genus Aeromonas as decolorants in the dyes.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a decolorizing agent for a dye selected from a slender dye, a disperse dye and a reactive dye contained in soluble colored wastewater, a method for decolorizing the same, and a microorganism which can be used in the method.

[0002]

2. Description of the Related Art Decolorization of soluble colored wastewater discharged from specialty paper mills, textile dyeing factories, etc. is incomplete with conventional microbial treatment equipment, and advanced treatment methods such as adsorption of oxidizing agents and activated carbon are added. Is done by doing.

[0003] However, in these wastewaters, there are sllen dyes, disperse dyes, which are hardly decomposed by ordinary microorganisms.
Since various kinds of reactive dyes and the like are contained, even if the above-mentioned means is used, it is difficult to perform a rapid treatment.

Japanese Patent Application Laid-Open No. 10-323185 discloses a carrier for immobilizing decolorized bacteria, which is obtained by growing and immobilizing bacterial species obtained by separating and storing only effective decolorizing bacterial species from the bacterial flora in activated sludge. I have. However, although this carrier has a high decomposition rate of wastewater pigment, its decomposition rate is not always sufficient, and in order to achieve a high decomposition rate, microorganisms are supported on the carrier and a large amount of bacteria is administered. I needed to. Furthermore, even with these bacteria, it was impossible to decompose slender dyes, disperse dyes and reactive dyes mainly used for dyeing fibers.

[0005] Therefore, there has been a demand for a decolorizing agent which decomposes a slender dye, a disperse dye and a reactive dye more rapidly and at a higher decomposition rate.

[0006]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide a dye which is difficult to be decomposed by ordinary microorganisms among dyes contained in soluble colored wastewater from a textile dyeing factory.
An object of the present invention is to provide a microorganism having excellent dissolving ability of disperse dyes and reactive dyes, a decolorizing agent containing the same, and a decolorizing method using the same.

[0007]

Means for Solving the Problems In view of such circumstances, the present inventors have conducted intensive studies and have found that microorganisms belonging to the genus Klebsiella, the genus Flavobacterium and the genus Aeromonas have excellent resolution of the dye. Heading, the present invention has been completed.

That is, the present invention relates to Kleb
The present invention provides a decolorizing agent for a dye selected from a slen dye, a disperse dye and a reactive dye containing one or more microorganisms selected from the genus siella), the genus Flavobacterium and the genus Aeromonas. It is. Further, the present invention provides a method of treating a microorganism selected from one or more dyes selected from a slender dye, a disperse dye, and a reactive dye, and a dye selected from a sllen dye, a disperse dye, and a reactive dye. The present invention provides a method for decolorization.

Further, the present invention provides a microorganism belonging to Klebsiella pneumoniae, Flavobacterium sp. Or Aeromonas sobria, and having a decolorizing ability of a dye selected from a slen dye, a disperse dye and a reactive dye. .

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION In the present invention, a suren dye is a vat dye having a condensed polycyclic quinone skeleton and excellent in fastness, and specifically, anthraquinone dyes such as indanthrone and thioindigo. Derivatives and the like. Commercial products such as Nihonthrene (manufactured by Sumitomo Chemical Co., Ltd.) and Indanthren (manufactured by Dystar) can be used as the slen dye.

The disperse dye is a nonionic dye having no ionic hydroxyl group such as sulfonic acid and carboxyl group.
Since it has a low solubility in water, it is formed into a fine powder and dispersed in water with a dispersant to be used for dyeing synthetic fibers. Specific examples include monoazo dyes, aminoanthraquinone dyes, and diphenylamine dyes.
As the disperse dye, for example, commercially available products such as Sumikaron (manufactured by Sumitomo Chemical), Foron (manufactured by Clariant), and Dianix (manufactured by Dystar) can be used.

The reactive dye is a dye having a reactive group which forms a covalent bond by reacting with an active group such as a hydroxyl group of a fiber in an aqueous solution containing an alkali. The reactive group is a cyanuryl chloride group. , Β-chloroethylsulfonyl, vinylsulfonyl and the like. As reactive dyes,
For example, commercially available products such as Sumifix, Sumifix Supra, Sumifix HF (all manufactured by Sumitomo Chemical Co., Ltd.), Drimaren X, Drimaren K (all manufactured by Clariant), Remazol, Reamova (all manufactured by Dystar) can be used. .

The decolorizing agent and the microorganism used in the decolorizing method of the present invention include Klebsiella spp., Flavobacterium spp. And Aeromona spp.
s) One or more microorganisms selected from the genus, especially Klebsiella pneumoniae
umoniae), Flavobacterium sp (Flavobact
erium sp. ) or Aeromonas sobria
obria), and Klebsiella pneumoniae Su-1, Klebsiella pneumoniae Su-
7, Klebsiella pneumoniae Bu-4, Flavobacterium sp. Bu-6-1, Flavobacterium sp. Ha-4, or Aeromonas sobria Ha
-2 is preferable because the resolution of the dye is high.

The above six strains are strains newly isolated from the sludge of a special paper mill by the present inventors and have the following mycological properties. In Table 1, the motility, Gram stainability and morphology of the bacterium were observed under a microscope.
wan and Steel's Guide to Identification of Medical Bacteria <Third Edition> ”Modern Primary Press, 1993. In addition, API 20 and API 20NE of the simple identification kit
Was used for identification.

[0015]

[Table 1]

[0016]

[Table 2]

* Api 20 (manufactured by Biomerieux Biotech Japan): Api 20 is a kit for identifying enteric bacteria and other gram-negative bacilli. 2 on one plate
0 microtubes and contains the dry substrate required for biochemical property testing. After inoculating each microtube with the bacterial solution of the test bacterium, the cells are cultured at 37 ° C. for 24 hours, and the spontaneous reaction of each microtube and the color reaction due to the addition of the reagent are read using a judgment table, and the aspirabo system (bacterial Identification software).

[0018]

[Table 3]

* Api20NE (manufactured by Biomerieux Biotech Japan): Api20NE is a kit for identifying Gram-negative bacilli other than intestinal bacteria. It has 20 microtubes on one plate and contains 8 items required for biochemical property tests and 12 items required for assimilation tests. After inoculating each microtube with the bacterial solution of the test bacterium, the cells were cultured at 30 ° C. for 24 hours, determined in the same manner as API 20, and identified.

<Identification Results (Results from API (Simplified Identification Kit))> Su-1: Klebsiella pneumoniae (% Id = 97.6) Su-7: Klebsiella pneumoniae (% Id = 98.0) Bu-4: Klebsiella pneumoniae (%) Id = 97.6) Ha-2: Aeromonas sobria (% Id = 99.2)% Id: Probability indicating how much the obtained numerical profile matches the bacterial species selected from the database.

Based on the above mycological properties, Bergey's Manual of Determinative Bacteriol
ogy) As a result of comparison with reference to the eighth edition, it was found that these strains were new strains.
The strains were deposited on June 14, 2000 and July 13, 2000 with the Research Institute of Biotechnology, National Institute of Advanced Industrial Science and Technology (accession numbers are FERM P-17964 (Su-7) and FERM, respectively).
P-17906 (Bu-4), FERM P-17904
(Ha-2), FERM P-17905 (Ha-4)).

The microorganism of the present invention decolorizes slender dyes, disperse dyes, and reactive dyes. The decolorizing ability of these dyes 5
In a CGY medium containing ５０50 ppm, one platinum loop was placed at 28 ° C. for 72 hours.
It can be evaluated by measuring the change in the absorbance of the culture supernatant after culturing for a period of time. More specifically, it is preferable to evaluate the decolorizing ability at 15 ppm for a slen dye, 5 ppm for a reactive dye, and 50 ppm for a disperse dye. . The microorganism of the present invention reduces the absorbance of the dye to 30% or more. The absorbance to be measured varies depending on the dye. For example, 500 nm for a slen dye, 520 nm for a disperse dye, 600 nm for a reactive dye, or a value around these can be used.

When a bacterium decomposing a dye selected from the genus Klebsiella, the genus Flavobacterium and the genus Aeromonas is used alone or in combination, it is possible to decompose the sllen dye, the disperse dye and the reactive dye safely, promptly and inexpensively. . For this reason, if the present strain is administered to wastewater from a paper mill, textile factory, or the like, the treatment of the wastewater can be performed safely, promptly, and at low cost.

As a method of wastewater treatment, for example, there is a method in which a carrier on which bacterial strains are attached at a high density is flowed by aeration air, the wastewater is brought into contact, and then separated from the carrier to obtain treated wastewater. At this time, the concentration of the bacterial cells to be administered may be appropriately set according to the load of the treatment wastewater, etc., and usually, from the viewpoint of speeding up the treatment speed and cost, workability, etc.
Preferably, about 20% of the volume of the treatment tank is immobilized.

As the administration form, the cells may be administered as they are, but it is preferable to use the cells in which the cells are supported on a carrier. The carrier used is not particularly limited,
Porous cellulose, PVA (polyvinyl alcohol),
Fine powdered activated carbon-coated PVA, activated carbon, (zeolite, barley stone, igneous rock, coral stone) and the like are preferable from the viewpoint of the ability to retain cells, and particularly, fine powdered activated carbon-coated PVA is preferable because of its high ability to immobilize microorganisms.

The immobilization of the strain on the carrier may be carried out by a known comprehensive immobilization method, a binding immobilization method, or the like. The amount of the cells to be supported on the carrier is not particularly limited, and is from 1 × 10 6 cfu / mL to
About 1 × 10 8 cfu / mL is preferable from the viewpoint of processing capacity.
The dose of the carrier may be determined in consideration of the above-mentioned dose of the cells and the amount of the cells carried.

[0027]

EXAMPLES Hereinafter, the present invention will be described in more detail by way of examples, but the present invention is not limited thereto. In the examples, the medium contained a carbon source and a dye, and a CGY medium having the following composition as it was or diluted to which a dye was added was used. In the case of an agar medium, it was added so that the agar content was 1.3% by weight.

[0028]

Example 1 (1) Confirmation of decolorization ability: CGY medium 100 was placed in a Sakaguchi flask.
1 mL of a sllene dye (Nihonthrene, manufactured by Sumitomo Chemical Co., Ltd.) (dissolved in water to 15 g / L), 50 μL of a disperse dye (Sumikaron, manufactured by Sumitomo Chemical Co., Ltd.), and a reactive dye (Sumifix, Sumitomo Chemical Co., Ltd.) 5 μL). Returned sludge collected from a treatment plant in Tokyo was added to the medium at a rate of 10 mL. A system to which no sludge was added was used as a control.
After shaking these at 28 ° C for 72 hours,
After centrifugation at 00 rpm for 10 minutes, the supernatant was filtered with a 0.22 μm filter, and the absorbance was measured with a spectrophotometer. The highest absorbance of each dye (slen dye; 500 nm,
(Disperse dye; 520 nm, reactive dye: 600 nm), the decolorization rate was determined, and the decolorization ability was confirmed for all dyes. Table 4 shows the results.

[0030]

[Table 4]

(2) Isolation of decolorized bacteria: The culture solution obtained in (1) was appropriately diluted, applied to a CGY agar medium, and
C. for 24 hours. Among the grown colonies, all that could be judged to be different in shape were picked, and inoculated into 2 mL of CGY medium supplemented with the dye in the same manner as in (1), and cultured at 28 ° C. for 72 hours. From the culture solution in which decolorization was confirmed, a loopful of platinum loops was collected and streaked on a CGY agar medium. A single colony that grew after 24 hours was picked, and CG with dye was added.
2 mL of Y medium was inoculated and allowed to stand still at 28 ° C. for 48 hours.
A culture solution with high decolorization ability is selected, one platinum loop is picked, and CGY
Streaked on an agar medium. Next, the passage was repeated 3 to 5 times to isolate the dye-degrading strains, and the 6 strains shown in Table 5 were isolated. The decolorizing ability of the isolated strain was confirmed by the same method as in (1).

[0032]

[Table 5]

[0033]

The use of the decolorizing agent using the microorganism of the present invention makes it possible to quickly and sufficiently decompose and sufficiently decolorize a slender dye, a disperse dye and a reactive dye.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C12R 1:01) C12R 1:01) (C12N 1/00 (C12N 1/00 PC12R 1:20) C12R 1:20) (C12N 1/00 (C12N 1/00 P C12R 1:22) C12R 1:22) (C12N 1/20 (C12N 1/20 A C12R 1:01) C12R 1:01) (C12N 1 / 20 (C12N 1/20 A C12R 1:20) C12R 1:20) (C12N 1/20 (C12N 1/20 A C12R 1:22) C12R 1:22) (72) Inventor Hiroshi Omura Minato-ku, Tokyo 1-119 Shimbashi Yakult Honsha Co., Ltd. (72) Inventor Maho Shimizu 16-1 Yanagimachi, Shizuoka City, Shizuoka Prefecture Abegawa Paper Mills Co., Ltd. (72) Inventor Toshiyuki Kawamura 16 Yanagimachi, Shizuoka City, Shizuoka Prefecture- 1 Abe River Paper Co., Ltd. in the F-term (reference) 4B065 AA01X AA27X AA29X AC12 AC20 BA22 CA55 4D040 DD01 DD11

Claims (6)

[Claims] 1. A dye selected from a slen dye, a disperse dye and a reactive dye containing one or more microorganisms selected from the genus Klebsiella, the genus Flavobacterium and the genus Aeromonas. Decolorizer. 2. The method according to claim 1, wherein the microorganisms are Klebsiella pneumoniae, Flavobacterium sp. And Aeromonas sp.
2. One or more kinds selected from Sovria.
The decolorizing agent as described. 3. Klebsiella (Kl) is used as one or two or more dyes selected from a slen dye, a disperse dye and a reactive dye.
ebsiella), Flavobacterium
A method for decolorizing a dye selected from a slen dye, a disperse dye, and a reactive dye, which comprises causing one or more microorganisms selected from the genus and the genus Aeromonas to act. 4. The method according to claim 1, wherein the microorganisms are Klebsiella pneumoniae, Flavobacterium sp. And Aeromonas sp.
4. One or more selected from Sovria.
The decolorization method described. 5. A microorganism belonging to Klebsiella pneumoniae, Flavobacterium sp. Or Aeromonas sobria, and having a decolorizing ability of a dye selected from a slen dye, a disperse dye and a reactive dye. 6. Klebsiella pneumoniae Su-7
(FERM P-17964), Klebsiella pneumoniae Bu-4 (FERM P-17906), Flavobacterium genus Ha-4 (FERM P-1790)
5) or Aeromonas sobria Ha-2 (FERM)
The microorganism according to claim 5, which is P-17904).