JP2001340071A - Sheetlike container and sheetlike culture medium using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain both a sheetlike container hermetically sealable without using an adhesive mass and capable of providing space saving and a sheetlike culture medium using the sheetlike container. SOLUTION: This sheetlike container is obtained by laminating a fine foamed sheet onto a film. The sheetlike culture medium uses the sheetlike container.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a sheet-shaped container and a sheet-shaped medium using the same.

[0002]

2. Description of the Related Art Conventionally, as containers for testing microorganisms,
A plastic Petri dish having a diameter of 90 mm and a height of 15 to 20 mm is used. By using such a Petri dish, a large culture space is required, and a problem that a large amount of plastic waste is generated is caused. Had.
Then, in order to solve these problems, in recent years, development of a microorganism culture medium using a sheet-like container has been studied. For example, WO 97/24432 discloses a microbial culture medium in which a porous matrix layer is sealed in a sheet-like container, and water or a sample solution is added to this layer to make a state in which microorganisms can grow. Have been. This microbial culture medium can be used for normal food inspection, and by directly contacting and wiping the porous matrix layer with the object to be inspected, the environmental inspection of microorganisms can be easily performed. This is a very excellent microorganism culture medium, for example, it can also suppress microorganisms. However, since the sheet-like container used for the microorganism medium requires an adhesive for sealing the container, a step of applying the adhesive at the time of manufacture is required, and the adhesive force is easily peelable. In this case, the re-adhesiveness is likely to be incomplete, and under low temperature conditions, the adhesiveness of the adhesive tends to decrease, so that there is a possibility of contamination by various bacteria, so that the processability, releasability and re-adhesion are improved. There has been a demand for the development of a sheet-like container having good properties and a sheet-like medium using the same.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a space-saving sheet-like container which can be sealed without using an adhesive and which can be easily peeled off and resealed, and a sheet-like medium using the same. is there.

[0004]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and as a result, have found that the following configuration achieves the intended purpose. The present invention has been achieved. (1) A sheet-like container in which a fine foam sheet and a film are laminated. (2) The sheet-like container according to the above (1), wherein the fine foam sheet is formed of a laminate of a fine foam layer and a backing sheet, and the fine foam layer has fine pores on its surface. (3) A sheet-shaped medium, wherein a culture layer is provided in the sheet-shaped container according to the above (1) or (2). (4) The sheet culture medium according to the above (3), wherein a part of the film constituting the sheet culture medium is configured to protrude from the fine foam sheet.

[0005]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
The sheet-like container of the present invention is formed by laminating a fine foam sheet and a film, and the film and the surface of the fine foam layer constituting the fine foam sheet adhere to each other without using an adhesive or a pressure-sensitive adhesive. At this time, the film needs to have a smooth surface. In addition, since the fine foam layer does not have adhesiveness, the fine holes function as small suction cups,
It is presumed that the film adheres to the film due to the attraction force of the micropores. From this, the sheet-like container of the present invention can repeatedly perform peeling and re-adhesion.

[0006] The adhesive strength of the fine foam layer can be adjusted by the thickness of the fine foam layer, the diameter and number of the fine pores on the surface of the fine foam layer. The average pore diameter of the micropores is 5 to 5
The range of 250 μm is preferable because of exhibiting good adhesiveness, and the range of 50 to 200 μm is more preferable. A fine foam layer having a foam having an average pore size of significantly less than 5 μm is difficult to manufacture, and thus is difficult to use industrially. Further, a fine foam layer having a foam significantly exceeding 250 μm tends to have extremely low adhesiveness.

[0007] Since the fine foam layer constituting the fine foam sheet has a suitable elasticity and good adhesiveness, it depends on the material of the fine foam layer. Is preferably 50 to 500 μm, more preferably 50 to 300 μm. When the thickness of the fine foam sheet is significantly less than 50 μm, the adhesive strength to a smooth surface decreases, and the cushioning property also tends to decrease,
Further, the rigidity is reduced. On the other hand, when the thickness greatly exceeds 500 μm, although the rigidity is increased and preferable, there is a disadvantage that the cost of the fine foam layer is increased and the obtained sheet-like container is expensive.

[0008] The fine foam layer is formed, for example, by mixing a mixed emulsion of a urethane resin emulsion, an acrylic resin emulsion and / or a butadiene-based synthetic rubber emulsion with air using an Oaks mixer or the like, applying this to a mount, and applying a heat treatment. It is obtained by doing. At this time,
The surface opposite to the surface attached to the backing sheet is the surface used for bonding.
In addition, a film, a sheet, a woven fabric, a nonwoven fabric, or the like made of a thermoplastic resin such as polypropylene or polyester is used as the backing paper.

The sheet-like container of the present invention can be widely used for applications requiring peeling and re-adhesion. Since the sheet-shaped culture medium requires peeling and re-adhesion and further sealing is required,
The sheet container of the present invention can be particularly preferably used. When a sheet-shaped medium is formed using the sheet-shaped container of the present invention, a culture layer may be provided between the microfoamed sheet and the film. The culture layer is provided by being adhered to a sheet-shaped container. If there is no particular problem, the culture layer may be simply placed without fixing. As the culture layer, for example, filter paper impregnated with nutrients for culturing microorganisms,
Water-soluble or water-absorbing polymer film, non-woven fabric and the like, and these may be used alone,
Further, they may be used in combination like a laminate of a nonwoven fabric and a water-soluble polymer compound film.

The medium component used in the present invention can be used without particular limitation as long as it is suitable for the microorganism to be detected. For example, nutrient components for culturing microorganisms are used. Further, an antibiotic such as an antibiotic that suppresses the growth of microorganisms other than for the purpose of detection, an antibiotic such as a synthetic antimicrobial agent, a coloring agent, a surfactant, or a selection agent such as an inorganic salt may be added. Further, a tetrazolim salt such as 2,3,5-triphenyltetrazolium chloride or a PH indicator may be added to the water-soluble polymer compound in order to make the formed colonies easily visible. A chromogenic or fluorescent enzyme substrate may be added.

As nutrients for culturing microorganisms, general-purpose liquid media or components obtained by removing agar from an agar medium can be used. For example, for general viable bacteria testing, yeast extract / peptone / glucose mixture, meat extract / peptone mixture, peptone / soybean peptone / glucose mixture and the like, and those obtained by adding dipotassium phosphate and / or sodium chloride thereto, For Escherichia coli and coliform testing, sodium desoxycholate, peptone, ammonium iron citrate, sodium chloride, dipotassium phosphate, lactose,
Neutral red mixture, peptone, lactose, dipotassium phosphate, eosin Y, methylene blue mixture, etc., for staphylococci, meat extract, peptone, sodium chloride, mannitol, phenol red, egg yolk mixture, peptone, meat extract, yeast extract・ Sodium pyruvate ・
A mixture of glycine / lithium chloride / tellurite egg yolk liquid is used for vibrio, yeast extract, peptone, sucrose,
Sodium thiosulfate, sodium citrate, sodium cholate, ferric citrate, sodium chloride, beef bile,
Bromthymol blue / thymol blue mixture is used for enterococci for bovine brain extract, heart extract, peptone,
Mixtures of glucose, dipotassium phosphate, sodium nitride, bromthymol blue, 2,3,5-triphenyltetrazolium chloride, etc., and for fungi, peptone / glucose mixture, yeast extract / glucose mixture, potato extract / glucose mixture, etc. Used.

As the film used for the sheet-shaped container and the sheet-shaped medium of the present invention, a film having a smooth surface can be most preferably used because of its excellent adhesion to the fine foam layer. When used as a sheet medium, the film is preferably composed of a material that does not inhibit the growth of microorganisms. As a film material,
For example, a thermoplastic resin such as polypropylene, polyethylene, or polyvinyl chloride can be used. When it is necessary to visually confirm the state of growth during the culture of the microorganism, a transparent film may be used. The thickness of the film is preferably in the range of 20 to 100 μm because it is flexible.

Hereinafter, a method for producing the sheet-shaped medium of the present invention and a method for using the same will be described.

(Preparation of a sheet-shaped medium) As a fine foam sheet (a laminate of a fine foam and a mount), a fine foam sheet composed of a fine foam layer / white polyester sheet was used.
A sheet-like container is formed by covering with a cover made of a polypropylene film and bringing the container into close contact.

(Preparation of culture layer) A solution composed of a 20% by weight aqueous solution of polyvinyl alcohol, a nutrient component for culturing microorganisms as a medium component, and distilled water was prepared. Finally, a polyvinyl alcohol solution is added), and this solution is coated on a polyethylene terephthalate sheet fixed to a glass plate using an applicator so as to have an appropriate basis weight, and a nonwoven fabric of the same size as the polyethylene terephthalate sheet is immediately formed. Laminated, 3 at 110 ° C
Let dry for minutes.

(Preparation of a sheet-shaped culture medium) The prepared culture layer was cut into a circular shape having a diameter of 5 cm, and the polyethylene terephthalate sheet side was removed from the central portion of the fine foam sheet (fine foam layer side) of the sheet container from which the cover was removed. Glued to
Cover again and make close contact with the fine foam sheet. Sterilize with ethylene oxide gas, γ-ray, etc. to obtain a sheet medium.

(Usage) A part of the film of the sheet medium is peeled off, a test solution is added to the culture layer, the film is re-adhered, and the culture is performed at an appropriate temperature.

[0018]

EXAMPLES Next, the present invention will be described specifically with reference to examples, but the present invention is not limited to the following examples.

Example 1 A fine foamed sheet having a thickness of 260 μm and a size of 80 m
mx90mm HISTAR-S manufactured by Techno Hirose Co., Ltd.
K (fine foam layer / white polyester sheet)
A cover of a polypropylene film having a thickness of 60 μm and a size of 90 mm × 90 mm was placed on the cover so as to protrude by 10 mm, and was brought into close contact with each other to obtain a sheet-like container. Next, 500 g of a 20% by weight aqueous solution of polyvinyl alcohol,
Meat extract 5 g, pepsin 15 g, NaCl 5 g, K ₂ H
A solution composed of 1 g of PO _4, 1 g of glucose, and 400 g of distilled water was prepared, and this solution was fixed on a glass plate.
A polyethylene terephthalate sheet of mm × 300 mm × 0.05 mm was coated using an applicator so as to have a weight of 115 g / m ² , a nonwoven fabric of the same size as the polyethylene terephthalate sheet was immediately attached, and dried at 110 ° C. for 3 minutes. The nonwoven fabric used here had an average thickness of 450 μm and an air permeability of 120 L / (m
² / sec) nylon melt blown nonwoven fabric. The culture layer thus produced was cut into a circle having a diameter of 50 mm, and the polyethylene terephthalate sheet side was
The cover of the sheet-like container was adhered to the central portion of the removed microfoam sheet, and the cover was again covered and adhered to the foam sheet. This was sterilized with ethylene oxide gas to obtain a sheet medium of the present invention. 10 g of various foods such as meat, cut vegetables, prepared dishes and the like were put into sterilized bags, 100 ml of water was added, and homogenized with a stomacher. To the obtained various food liquids, sterilized water was added to prepare a 10-fold diluted train. . From the sheet-shaped medium, the film was opened until the entire top surface of the culture layer was exposed, and 1 ml of a 10-fold diluted solution was added to the culture layer, covered again with the film, sealed, and cultured at 35 ° C. for 24 hours.

At a culture time of 24 hours, the culture layer in the sheet-shaped container was not dried. This indicates that the sheet container is closed. In addition, since a microbial colony was formed on the culture layer, it was found that the sheet-shaped medium can be used as a sheet-shaped medium.

[0021]

The sheet-like container of the present invention is excellent in processability and productivity because there is no need to apply an adhesive during the production of the container, and at the same time, it can be easily sealed as a container without using an adhesive. Available and excellent. Further, the sheet-like container of the present invention can repeat peeling and re-adhesion that cannot be performed with an adhesive, and does not cause a decrease in adhesiveness in a low-temperature environment that cannot be performed with an adhesive. Furthermore, since it is sheet-shaped and low in bulk, it is excellent in that the culturing space can be significantly reduced and the bulk of waste can be reduced at the same time.

Claims (4)

[Claims] 1. A sheet-like container formed by laminating a fine foam sheet and a film. 2. The sheet-like container according to claim 1, wherein the fine foam sheet comprises a laminate of a fine foam layer and a backing sheet, and the fine foam layer has fine pores on its surface. 3. A sheet-shaped medium, wherein the sheet-shaped container according to claim 1 is provided with a culture layer. 4. The sheet culture medium according to claim 3, wherein a part of the film constituting the sheet culture medium protrudes from the fine foam sheet.