JP2001328916A - Nematode preparation and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a nematode preparation capable of preserving a nematode in a high survival rate of the nematode, and capable of being applied to the large nematode. SOLUTION: (1) This nematode preparation comprises the nematode having biological ability for controlling a pest, and a wood flour, or the nematode and a carrier consisting essentially of the wood flour. The nematode preparation preferably has >=40 wt.% water content, and the particle diameter of the wood flour is preferably <=300 μm. (2) The method for producing the nematode preparation is characterized in that the nematode and the water in an amount regulated so that the water content may be >=40 wt.% are added to the carrier consisting essentially of the wood flour, or the carrier consisting of a mixture of the wood flour as the essential component with an inorganic material power and/or a disperser, and the resultant mixture is mixed.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a nematode preparation containing a nematode which is parasitic on a pest and can be biologically controlled. More specifically, the present invention relates to a nematode preparation having excellent preservability, especially for large nematodes, excellent dispensability, applicability, and the like, and a method for producing the same.

[0002]

BACKGROUND OF THE INVENTION C. elegans are a taxonomic nematode ( Nema
toda ), and is roughly classified into two networks: a twin network and a twin gland network.
The former are mostly animal and plant parasites, while the latter are many species that live freely in soil and water. Currently about 1
Although 5,000 species are known, the total number of species is estimated to exceed 500,000. In this, bi gland network, the nematodes belonging to桿線insect Amo (Rhabditia) contains parasitic nematodes insects called "insect parasitic" or "entomopathogenic" nematode pests of organisms It has been regarded as a promising chemical control agent for a long time, and many studies have been conducted for practical use. Slug-killing nematodes that infect and kill harmful animals such as insect-parasitic nematodes and slugs are useful as excellent biological control agents in the fields of agriculture, forestry, termite control, and sanitation. It is widely known.

[0003] Insect-parasitic nematodes have a wide host range but are highly safe for non-target animals and the environment, are easy to mass culture and apply, and are effective as a substitute for many chemically synthesized insecticides. But it is excellent. At present, these insect parasitic nematodes are commercialized on a worldwide scale in many crop cultivation fields such as citrus, strawberry, cranberry, turf, mushroom and greenhouse horticulture. In addition, slug-killing nematodes are also used in the fields of gardening and greenhouse horticulture.

[0004] After mass production (culture), the infectious larvae of the above nematodes can be stored at low temperatures (refrigerated) for several days to several weeks while being aerated as a suspension. When the density becomes high (high concentration), the survival rate and quality of the nematodes are greatly reduced due to a decrease in dissolved oxygen and the occurrence of mold. Therefore, it is usually necessary to formulate the preparation immediately after culturing. Characteristics required for nematode preparations include that they can be stored for a long time while maintaining a high nematode survival rate, that they can have a high nematode concentration, that they are easy to formulate, and that they have excellent workability when applied to the field. However, various nematode preparations have been proposed or marketed.

[0005]

2. Description of the Related Art Conventionally known nematode preparations include:
There are (1) preparations in which nematodes are held by an inert substance such as polyester-polyurethane sponge and powdered vermiculite, and (2) preparations using various active substances. The following are known as those utilizing active substances. (2-1) Alginate gel preparation gelled with calcium alginate, (2-2) Flowable gel preparation with increased viscosity by adding a thickener, (2-3) Activated carbon with synthetic resin and fungicide to nematodes The added creamy formulation, (2-4) nematodes were mixed with clay to remove excess water on the surface, and a partially dehydrated and dried clay sandwich formulation, and (2-5) nematodes as flour gluten And granulated pesto, (2
-6) Water-dispersible granules (WDG) for enclosing nematodes in particles having a diameter of 1 to 2 cm of a mixture of silica, clay, cellulose, lignin and starch; A wettable powder formulation containing a fungicide and having improved preservability, and (2-8) a liquid formulation comprising a high concentration nematode suspension and a metabolic inhibitor and an antibiotic.

[0006]

Inert substances such as sponges and vermiculite are usually used to store nematodes on a small scale under refrigerated conditions, and 50,000 to 250 in one container.
The limit is 100,000 heads, and minerals such as vermiculite have limited water retention, fast drying speed, 1m long
Not suitable for large nematodes of m or more. Steinernema carpocapsa
e ), Steinernema f
eltiae ), Heterorhabditis bacteriophora , Heterorhabditis megidis , and Phasmarhabditis genus have been formulated, but cannot be stored at room temperature. Steinernema Feltiere ( Steinern)
The shelf life of ema feltiae is as long as 4-5 months.

[0007] (2-1) Alginate gel preparation using an active substance is obtained by solidifying a nematode on a thin plastic sheet, and dissolving the gel by adding sodium citrate at the time of application to elute the nematode. It is. Although it can be stored at room temperature for 3 to 4 months, it is not used at present because of the drawbacks of labor and time for spraying and difficulty in holding a large amount of nematodes.

[0008] (2-2) flowable gel formulation, Sutainanema-Karupokapusae (Steinernemacarpocapsae), Sutainanema-Ferutiae (Steinernema feltia
e ) Invented for small to medium-sized nematodes, such as nematodes. However, due to the high concentration of nematode suspensions, the mortality rate increases due to insufficient supply of dissolved oxygen, and the storage period is short. In addition, it is necessary to put a highly viscous solution thinly and widely in a package, and the preparation container becomes large and lacks versatility. Slurry preparations using polyacrylamide and slurry preparations using an insoluble solid (silica) (Japanese Patent Application Laid-Open No. 7-33618) have also been proposed, but the number of nematodes retained is small and the product life is short. There is a disadvantage that it is not distributed.

(2-3) A creamy preparation prepared by adding activated carbon to a nematode with a synthetic resin and a bactericide (Japanese Patent Application Laid-Open No. 61-501392) is expensive, is difficult to handle, and has been put to practical use due to its shelf life and the like. It has not been.

(2-4) The clay sandwich preparation is temporarily
Commercialized, low storage at room temperature, low solubility,
It has not been distributed recently because the nozzle of the sprayer is easily clogged and the number of nematodes is smaller than the ratio of clay.

(2-5) The pesta preparation contains an additive and a wetting agent, but is dried to lower humidity for the purpose of suppressing the movement of nematodes and suppressing the growth of other microorganisms. Are hard to dissolve in water, and eventually the nematode survival rate is low.

(2-6) Water-dispersible granules (WDG) are currently mainly distributed, and are used to convert nematodes into a mixture of silica, clay, cellulose, lignin and starch having a diameter of 3 to 5 m.
m, and is formed by granulating bread while dropping a high-concentration nematode suspension onto fine dry powder. After the granulation, the nematodes are gradually dried under a certain appropriate temperature condition and partially dehydrated. Four to seven days after granulation, the nematodes reduce their own oxygen consumption and thus have a longer shelf life. The storage period of WDG is 4 months at room temperature in Steinernema carpocapsae ,
It can maintain a survival rate of 80% or more for 8 months in refrigeration, and does not require special transportation costs such as refrigerated transportation. However, Steinernema
feltiae ) and Steinernema Rio Bravis ( Steinerne)
ma riobravis ) for 2-3 months.

[0013] The initial moisture content of WDG is adjusted to a standard of 40-42%, and Steinernere carpocapsae is used.
nernema carpocapsae ) 300,000 to 40 per gram
Can hold ten thousand nematodes. However, dehydrated nematodes are inactive and cannot start vigorous movement unless suspended in water for a while. Therefore, if WDG is suspended in water and sprayed immediately after water, the water is absorbed into the soil, the water cannot be absorbed sufficiently into the nematode body, and the number of effectively working nematodes cannot be secured. In particular, this occurs significantly in formulations that have been stored for a long time. In addition, the size of the nematode confined in the WDG preparation is suitable for small nematodes having a body length of 560 to 700 μm, such as Steinernema carpocapsae and Steinernema riobravis, and is suitable for Steinernema glaseri . Large nematodes having a body length of 1 to 1.2 mm or more are not suitable for WDG because they have high motility and the grains are disintegrated, and the amount of oxygen consumed is so large that the preservability cannot be extended.

(2-7) A wettable powder formulation containing a dispersant and other additives to improve the preservability based on a carrier of a mineral substance such as vermiculite and clay is used to improve the preservability of the product by using Steinernema carpocapsae at room temperature. carpocapsa
e ) Steinernema rio Bravis
bravis , Heterolabditis Megidis ( Hetero)
rhabditis megidis ) can be stored for 2.5 to 3.5 months and refrigerated at 5 ° C for 6 to 8 months, but this formulation is also suitable for small nematodes and consumes much oxygen, such as Steinernema glaseri . Large nematodes cannot be preserved longer.

[0015] Steiner Nema Kushidai ( Steine)
As a wettable powder for rnema kushidai ), if nematodes are directly mixed with a carrier such as clay, rapid dehydration will occur. It has been proposed to contact the insects with clay and then remove the water absorber by sieving (WO97 / 151).
93). According to this wettable powder, a high survival rate of 5 days at 120C is obtained. However, although the contact between the nematode and the water absorbent is effective for maintaining the physiological state of the nematode well, it has an essentially useless process of finally removing the water absorbent once mixed. , Increasing manufacturing costs. In addition, there is a problem that the water-absorbing body cannot be completely removed and remains in the preparation.

(2-8) A liquid preparation obtained by adding a metabolic inhibitor and an antibiotic to a high-concentration nematode suspension is useful as a method for transporting a large amount of nematodes at low cost.・ Approximately 3 Steinernema carpocapsae
50,000 to 400,000 / gram can be stored at room temperature for 6 days and refrigerated for about 15 days. However, it is not suitable for storing large nematodes, which consume a large amount of oxygen and quickly become deprived of oxygen.

The greatest cause of death in nematode preparations is
It has been found that the nematode individual is dehydrated and dried by drying the preparation. Therefore, it is preferable that the water content in the preparation is high, but when a mineral substance is used as a carrier, the water content allowable as a water-containing wettable powder is at most in the lower 40% range (W /
W). This is a problem that is difficult to solve because of the low water retention ability of minerals and the like, and the fact that once the minerals are dried, the drying spreads rapidly throughout the preparation. Water-dispersible granules (WDG) have solved this problem.
However, there is a problem that it cannot be applied to medium to large nematodes as described above.

Therefore, an object of the present invention is to make the body length 0.8 mm.
It is an object of the present invention to provide a nematode preparation which can increase the nematode concentration even for medium to large nematodes having a length of 1 mm or more and is excellent in long-term storage properties, formulation properties, applicability and the like.

[0019]

Means for Solving the Problems The present inventors have focused on wood flour as a material having sufficient water-containing ability and durable for drying. As a result, when using wood flour alone or using wood flour as a main component in combination with other mineral carriers, and mixing and formulating nematodes, a stable formulation with a high moisture content was obtained. The present inventors have found that a nematode preparation having significantly improved storage stability of medium to large nematodes can be obtained, and furthermore, the simplicity of application has been confirmed and the present invention has been completed.

That is, the present invention provides the following nematode preparation and a method for producing the same. 1) A nematode preparation comprising a nematode capable of biologically controlling pests and wood flour. 2) A nematode preparation comprising a nematode capable of biologically controlling pests and a carrier containing wood flour as a main component. 3) The nematode preparation according to 1 or 2 above, wherein the water content is 40% by mass or more. 4) The above 2 wherein the ratio of wood flour in the carrier is 50% by mass or more.
2. The nematode preparation according to item 1. 5) The nematode preparation according to any one of 1 to 4 above, wherein the particle size of the wood flour is 300 μm or less. 6) The carrier component as described in 2 above, wherein the carrier component other than wood flour is a mineral powder.
The nematode preparation according to any one of claims 1 to 5, wherein 7) The nematode preparation according to any one of the above 1 to 6, further comprising a dispersant and / or a fungicide. 8) The nematode preparation according to any one of the above 1 to 7, wherein the nematode is a nematode belonging to the order Rhabditida . 9) A nematode belonging to the order Rhabditida is an insect-parasitic nematode belonging to the family Steinernema or Heterorhabditis or a slug killing property belonging to the genus Phasmarhabditis . The nematode preparation according to the above item 8, which is a nematode. 10) Steinernema family nematodes, Steinernema carpocap
sae , Steinernema
feltiae ), Steinernem Graselai ( Steinernem)
a glaseri ), Steiner Nema Rio Bravis ( Steiner)
nema riobravis ) or Steinernema kushidai . 11) hetero Love de Sevilla (Heterorhabditis) Department of nematodes is, hetero Love di Sevilla-bacteriophage follower La (He
10. The nematode preparation according to the above 9, which is terorhabditis bacteriophora ) or Heterorhabditis megidis . 12) The nematode preparation according to the above item 9, wherein the nematode belonging to the genus Phasmarhabditis is Phasmarhabditis hermaphrodita . 13) A carrier composed of wood flour or a carrier composed of a mixture of wood flour, mineral powder, and a dispersant and / or fungicide of the main components, nematodes and water having an amount of water of 40% by mass or more. And mixing the resulting mixture.

Hereinafter, the present invention will be described specifically. (1) Carrier In the present invention, as a carrier for preserving nematodes, wood flour is used alone, or wood flour is used as a main component in combination with another mineral carrier. Wood flour is prepared by drying ordinary trees as required and then pulverizing them. The type of tree is not particularly limited, but needles and hardwoods such as fir and pine which are inexpensive and easily available are preferred. The particle size of the wood flour used should be such that the nozzle of the sprayer does not clog at the time of application, and the maximum particle size measured by particle size distribution is preferably 300 μm or less, more preferably 200 μm or less. The average particle size is preferably from 20 to 150 μm, more preferably from 50 to 100 μm. Wood flour ratio of 5 when used with other minerals
0 mass% or more is preferable, 60 mass% or more is more preferable, and 70 mass% or more is more preferable. If the proportion of wood flour is small, the water retention capacity of the preparation is insufficient, and the survival rate of nematodes is reduced. Other minerals to be used in combination are not particularly limited as long as they do not hinder the water retention ability of wood flour, but, for example, clay, silica, laurite, talc, kaolin, calcium carbonate, bentonite, silica stone powder, limestone powder, acid clay 50% by mass of diatomaceous earth powder, gypsum, pumice powder, shellfish powder, mica powder, colloidal hydrous sodium silicate, etc.
Hereinafter, it can be preferably used in the range of 20 to 40% by mass.

(2) Nematodes The nematodes used in the nematode preparation of the present invention can control pests biologically. Examples include insect parasitic nematodes or nematodes parasitic on insects called entomopathogenic nematodes, and slug-killing nematodes that infect and kill harmful animals such as slugs. Specifically, rodworm ( Rhabditida )
Nematodes belonging to Steiner Nema ( St.
Einernema or Heterabhaditis ( Heterorha)
bditis ) is an insect parasitic nematode belonging to the family, or a slug-killing nematode belonging to the genus Phasmarhabditis . Insect parasitic nematodes belonging to the Steinernema family include Steinernema carpocapsae , Steinernema feltiae , Steinernema glaseri , and Steinernema avira visa.
s ), Steinernema kushida
i ) and the like, Heterorhabditis ( Heterorha)
Insect-parasitic nematodes belonging to the family bditis ) include Heterorhabditis ba
cteriophora ), heterolabditis magidis ( H
terorhabditis megidis ), and slug-killing nematodes belonging to the genus Phasmarhabditis include Phasmarhabditis hermaphrodita . Nematodes formulation of the present invention can be used without limiting the foregoing nematode, especially body length of about 0.8mm Medium nematode (Sutainanema-Ferutiae (Steinernema felt
iae )), and a large nematode of 1 mm to 1.5 mm in length ( Steinernema glaseri ),
It is also applicable to Phasmarhabditis .

(3) Additives In the nematode preparation of the present invention, various additives can be blended. Examples of additives that can be used include, for example, dispersants, fungicides, wetting agents, anti-caking agents, humectants, and the like.
It is not limited to these. Examples of the dispersant include alkyl sulfate, polyoxyethylene (POE) alkyl ether sulfate, POE alkyl phenyl ether sulfate, POE benzylation (or salification).
Phenyl (or phenylphenyl) ether sulfate, paraffin (alkane) sulfonate, α-olefin sulfonate (AOS), alkylbenzene sulfonate, mono- or dialkylnaphthalene sulfonate, naphthalene sulfonate / formalin condensate, alkyl diphenyl ether disulfonate, lignin sulfonate, POE alkyl ether Sulfosuccinic acid half ester, POE benzylated (or styrylated)
Phenyl (or phenylphenyl) ether phosphate and the like. 7% by mass in the carrier
Or less, preferably 5% by mass or less. As a fungicide, a paraoxybenzoic acid derivative, saltyl anilide,
1,2-benzisothiazolin-3-one, tetrachloroisophthalonitrile (TPN), 2-nitrobromo-
1,3-diol and the like. The compounding amount is usually about 1% by mass or less in the preparation. As a humectant,
POE alkyl ether, POE alkyl phenyl ether, POE dialkyl phenyl ether, POE alkyl amine, dialkyl sulfosuccinate, and the like.

(4) Preparation Method of the Preparation The nematode preparation of the present invention is prepared by culturing a nematode prepared on a carrier consisting of wood flour alone or a carrier obtained by mixing wood flour with other minerals or dispersants. Insects and mechanically stir water and other additives (antifungal agent, etc.) in an amount to keep the moisture content of the preparation at a desired value,
It can be prepared by a very simple method of mixing. When the water content of the carrier and the nematode is high, it is not necessary to add water, but the water content of the preparation is 40% by mass or more, preferably 45% by mass or more, more preferably 50% by mass or more.
Water is added to adjust the water content so as to be mass%.

In the preparation of the present invention, the water content is favorably supported on wood flour to a water content of 50% by mass or more and about 60 to 65% by mass. Not be. It is preferable that the nematodes are mixed so as to be 30 to 70% by mass in the preparation. A more preferred mixing amount is 40 to 60% by mass. If the amount of the nematode is too large, the survival rate of the nematode in the preparation decreases. According to nematodes formulations of the invention thus produced, Sutainanema-Guraserai (Steinernema glaseri), 200,000 to 50 million head nematodes per formulation 1g in large nematodes such as Fast Maraboe di infantis (Phasmarhabditis) Can be stored for a long time. The nematode preservation period of the preparation of the present invention which can maintain the nematode survival rate at 80% or more is about 4 to 6 months at a temperature of 5 ° C. or less, and about 1 to 2 months at room temperature, which is a practically sufficient period. Can store nematodes.

(5) Application Method When the nematode preparation of the present invention is applied to a field, the nematode preparation is dispersed in water and sprayed using a sprayer or the like. The preparation of the present invention is a dry powder preparation, and can be rapidly dispersed in water. Usually, the dispersion concentration is appropriately set in the range of 10 to 1000 g of the preparation per 100 liters of water. With conventional water-dispersible granules (WDG), dehydrated nematodes are inactive, and it takes time to start exercising after suspending in water. However, there is a problem that the number of nematodes that work effectively cannot be ensured, but the nematodes in the preparation of the present invention are activated immediately after being suspended in water, so that they are excellent in workability in a field.

[0027]

EXAMPLES The present invention will be described in more detail with reference to examples and comparative examples, but the present invention is not limited to the following examples.

Example 1 Wood flour (Technocell (trade name), Cell
ulo-Fullostoff-Fabrik (import agency: Union Kasei Co., Ltd.), fir: pine = 8: 2, particle size of 300 μm or less, average particle size of 100 μm), and after adding water to make it uniform, Steinernema Graselei ( Steinernema) glaseri )
200 g (equivalent to 1.5 × 10 ⁸ ) of a nematode mass (average body length: 1.2 mm) was added and uniformly mixed with a stirring mixer to obtain a nematode formulation (formulations 1 to 5). About the obtained nematode preparation,
The water content and nematode concentration were measured. In addition, a nematode preparation was prepared in the same manner using clay (particle size: 20 to 50 μm) as a carrier (comparative preparations 1 to 3), and the water content and the nematode concentration were measured. Table 1 shows the results.

[0029]

[Table 1]

The prepared preparation was placed in a polypropylene tray, and an oxygen-permeable polyethylene sheet was heat-pressed and placed at 5 ° C. After storage, 30, 60, 12
On days 0 and 180, the container was opened, the number of living and dead nematodes was measured, and the survival rate was determined. Table 2 shows the results.

[0031]

[Table 2]

As is clear from Tables 1 and 2, the nematode preparation using clay has a low nematode survival rate, poor storage stability (comparative preparations 1 and 2), and cannot be formulated when the water content is high. (Comparative formulation 3). The nematode preparation of the present invention using wood flour as a carrier shows a good survival rate, and particularly has a water content of 40%.
The above preparations (Formulations 2 to 5) exhibited excellent survival rates even on the 180th day, indicating that the preparations were stable even at a high water content.

Example 2 Steinernema carpoc
apsae ) (average body length 558 μm) 75 g (1.5 ×
10 ⁸ animals equivalent), and using a wood flour or clay in proportions shown in Table 3, the nematodes formulation was prepared in the same manner as in Example 1, were measured moisture content and nematode concentration. Table 3 shows the results.

[0034]

[Table 3]

The prepared preparation was placed in a polyethylene tray, and an oxygen-permeable polyethylene sheet was heat-sealed and placed at 5 ° C. After storage, the container was opened on day 120, and the nematode activity rate and insecticidal activity were examined. The activity rate was obtained by suspending the nematode preparation in water and elapse of one hour, and then measuring the proportion of the actively moving nematodes to the investigated nematode individuals in the suspension. The measurement was repeated five times, and the average value was obtained. Insecticidal activity was measured for one terminal larva of honeysuckle ( Gallaria mellonella ) using a 24-well tissue culture plate and placing one nematode into a 1 cm diameter well of filter paper bed.
The mortality of the test insects was measured at 72 ° C. for 72 hours. In one experiment, 24 test insects were used, and this was repeated three times to obtain an average value. Table 4 shows the results.

[0036]

[Table 4]

It is apparent from Table 4 that nematodes are severely dehydrated when stored in a clay formulation for a long period of time, and have low activity even when they are alive, resulting in a decrease in insecticidal activity. On the other hand, in the case of the preparation of the present invention, the nematode has active activity even after long-term storage, and has high insecticidal activity.

[0038] Example 3 Six entomopathogenic nematodes (Sutainanema-Karupokapusae (Steinernema carpocapsae), Sutainanema-Ferutiae (Steinernema feltiae), Sutainanema-Rioburabisu (Steinernema riobravis), Sutainanema-Kushidai (Steinernema kushidai), Heterorabudi Hetisorhabditis
bacteriophora ) and Heterorhabditis megidis ) were dehydrated with a 20 μm mesh to form a slurry. Each of these 20g (4x
About 0.2 g (corresponding to a final concentration of 0.2%) of an antifungal agent (1,2-benzisothiazolin-3-one; hereinafter the same was used as an antifungal agent) was uniformly mixed with 10 ⁷ heads. 80 g of the carrier (28 g of wood flour, 12 g of vermiculite fine powder, and 40 g of water) were mixed with the nematode, and the unit nematode amount was set to 400,000 head / gram as the final preparation (preparations 7 to 12). . On the other hand, 37.5 g (3 × 1) of Phasmarhabditis hermaphrodita similarly dehydrated and made into a slurry.
0 ⁷ animals equivalent) to the fungicide about 0.2 g (final concentration of 0.2% equivalent) were uniformly mixed. 62.5 g of carrier (33.3 wood flour)
g, vermiculite fine powder (14.2 g) and water (15 g) were uniformly mixed.
It was set at 100,000 heads / gram (formulation 13).

As a comparative preparation, each of the above six entomopathogenic nematodes in the form of a slurry dehydrated with a mesh was 20 g (4 × 10 4
⁷ heads or equivalent) in a mixture of fungicide about 0.2 g (final concentration of 0.2% equivalent) was mixed with vermiculite fines 80g was uniformly added to water in advance 40 g, a unit nematode amount as the final preparation 40
It was 10,000 / gram (comparative preparations 5 to 10). Meanwhile, Phasmarhabditis hermaphrodita , also dehydrated and slurried
37.5 g (equivalent to 3 × 10 ⁷ ), about 0.2 g (corresponding to a final concentration of 0.2%) of an antifungal agent, 15 g of water and 62.5 g of vermiculite fine powder, which had been made uniform in advance, were mixed. The amount of nematodes was 300,000 / gram (Comparative Preparation 11). Each of the prepared preparations was stored in a polyethylene tray, sealed by heating and pressing a breathable film, and held in a low-temperature warehouse (5 ° C.). On the day after the start of storage, 30, 30, 61, 90, and 180 days after the start of storage, the prepared formulation was uniformly sampled from the formulation,
After appropriate dilution, nematode viability was counted under a microscope. Table 5 shows the results.

[0040]

[Table 5]

From Table 5, it can be seen that the wood flour preparation of the present invention maintained a high survival rate of 73 to 85% on any 180 days after 180 days, whereas only vermiculite fine powder was used. The nematode survival rate in the comparative formulation is 12-41%,
It is clear that the formulation of the present invention significantly improves the nematode survival rate.

Example 4 Entomopathogenic nematode ( Steine Nemar Gracerai)
rnema glaseri )) was dewatered with a 20 μm mesh to form a slurry. A mixture of about 1 g of the same fungicide as in Example 3 was added to this slurry nematode to make it uniform.
248.4 g of carrier was added to 248.6 g of this 1.6 × 10 ⁸ nematode equivalent.
(Wood flour 156.5g, vermiculite fine powder 67.1g, water 24.8
g of the final formulation was mixed to give a unit nematode amount of 330,000 / gram (Formulation 14). The prepared preparation was stored in a polyethylene tray, sealed by heating and pressing a breathable film, and stored in a low-temperature warehouse (5).
C). Similarly, 248.4 g of a slurry-like nematode dehydrated with a mesh and 248.4 g of a carrier obtained by mixing vermiculite fine powder with the same fungicide as in Example 3 are uniformly mixed to prepare a comparative preparation (comparative preparation 11). did. The preparation was stored and stored in the same container and film. On the day after the start of storage, 33 days, 61 days, 93 days, and 180 days after, perform uniform sampling from the preparation, and after appropriate dilution,
Nematode viability was counted under a microscope. Table 6 shows the results.

[0043]

[Table 6]

From Table 6, the survival rate of large nematodes was also determined.
It can be seen that the wood flour preparation of the present invention is significantly improved as compared with the comparative preparation using only vermiculite fine powder.

Example 5 A field test was conducted on the preparation 14 prepared in Example 4. The field test was conducted at the place where smadarakogane occurs at Mobara Country Club, Chiba Prefecture. 16 stored for about 2 months
6 g of Formulation 14 (equivalent to 50 million nematodes) was placed in a tank containing 400 liters of water to prepare a suspension. The scatter of 1m ² per 250,000 head this 200m ² (10 × 20
m). [Number and survival rate of nematodes in the tank and in the spray liquid] After the formulation was suspended in water in the tank, the nematode concentration of the suspension and the concentration in the spray liquid sprayed from the nozzle (nozzle diameter: 300 µm) were measured. The nematode concentration was investigated respectively. As shown in Table 7, it can be seen that the present formulation was uniformly suspended in the tank and was uniformly discharged without clogging from the tank to the nozzle.

[0046]

[Table 7]

[Effects of Control of the Preparations against Scutellariae] Before spraying the nematode liquid, 10 holes were extracted with a hole cutter having a diameter of 10 cm, and the density of the target pests before spraying was examined. About one month after the spraying, the same investigation was conducted to determine the pest density. As a non-treated area, the density of the portion not sprayed was also examined before spraying and one month after spraying.
Table 8 shows the results.

[0048]

[Table 8]

As is clear from Table 8, an average of 3.9 larvae were observed in the test plot before the spraying and an average of 5.5 larvae in the untreated plot, but no larvae were observed in the treated plot one month after spraying. On average, 2.8 were found in the untreated plot.

[0050]

According to the conventional nematode preparation using a mineral substance capable of preserving a small nematode for a long period of time, when the water content is increased to about 40% by mass, the holding power of the water is reduced and the preparation becomes saturated and becomes uniform. It was impossible to store large nematodes with high oxygen consumption at high concentrations, but according to the preparation of the present invention using wood flour, the water content was increased to about 40 to 60% by mass. Even a stable formulation can be obtained,
The preservability of large nematodes is greatly improved. Further, it can be easily prepared and can be easily applied, so that it is extremely useful in practice.

 ──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Hiroshi Tanabe 2-1-1 Midorigahara, Tsukuba-shi, Ibaraki FDS-term in the SDS Biotech Tsukuba Research Laboratories (reference) 4H011 AC01 BA01 BB23 BC19 BC20 BC22 DA02 DD04 DH10

Claims (13)

[Claims] 1. A nematode preparation comprising a nematode capable of biologically controlling pests and wood flour. 2. A nematode preparation comprising: a nematode capable of biologically controlling pests; and a carrier containing wood flour as a main component. 3. The method according to claim 1, wherein the water content is 40% by mass or more.
Or the nematode preparation according to 2. 4. The nematode preparation according to claim 2, wherein the ratio of wood flour in the carrier is 50% by mass or more. 5. The nematode preparation according to claim 1, wherein the particle size of the wood flour is 300 μm or less. 6. The nematode preparation according to claim 2, wherein the carrier component other than the wood flour is a mineral powder. 7. The nematode preparation according to claim 1, further comprising a dispersant and / or a fungicide. 8. The nematode preparation according to any one of claims 1 to 7, wherein the nematode is a nematode belonging to the order Rhabditida . 9. A nematode belonging to the order Rhabditida , wherein the worm is an insect parasitic nematode belonging to the family Steinernema or Heterorhabditis , or Phasmarhabdi.
The nematode preparation according to claim 8, which is a slug-killing nematode belonging to the genus tis ). 10. The Steinernema family of nematodes is Steinernem carpocapsae.
a carpocapsae ), Steinnerema Fertiae ( S
teinernema feltiae), Sutainanema-Guraserai (Steinernemaglaseri), nematode formulation as claimed in claim 9 is a Sutainanema-Rioburabisu (Steinernema riobravis), or Sutainanema-Kushidai (Steinernema kushidai). 11. Heterorhabdi
tis) Department of nematodes is, hetero Love di Sevilla-bacteriophage follower La (Heterorhabditis bacteriophora), or a hetero Love de Sevilla, Megidisu (Heterorhabditis me
gidis ). 12. Phasmarhabtis
ditis ) is a nematode belonging to the genus Fasmarabditis
Hermaphrodita ( Phasmarhabditis hermaphrodit)
The nematode preparation according to claim 9, which is a ). 13. A carrier consisting of wood flour, or a carrier consisting of a mixture of wood flour, mineral powder, dispersant and / or fungicide as main components, has a nematode and water content of 40% by mass or more. A method for producing a nematode preparation, comprising adding and mixing an amount of water.