JP2001321156A5 - - Google Patents
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- JP2001321156A5 JP2001321156A5 JP2000143032A JP2000143032A JP2001321156A5 JP 2001321156 A5 JP2001321156 A5 JP 2001321156A5 JP 2000143032 A JP2000143032 A JP 2000143032A JP 2000143032 A JP2000143032 A JP 2000143032A JP 2001321156 A5 JP2001321156 A5 JP 2001321156A5
- Authority
- JP
- Japan
- Prior art keywords
- tetrazolium
- manufactured
- final concentration
- electron donor
- methoxy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Description
呈色試薬としては、酸化還元指示薬と電子供与体が用いられる。酸化還元指示薬としては、還元されることによって呈色するテトラゾリウム塩で、生成するホルマザンが水溶性のものが好ましく、例えば２−（４−ヨードフェニル）−３−（４−ニトロフェニル）−５−（２，４−ジスルフォフェニル）−２Ｈ−テトラゾリウム・ナトリウム塩（同仁化学研究所社製、ＷＳＴ−１）等を好適に使用することができる。これらのテトラゾリウム塩は、使用終濃度が０．０５〜０．５mM、特に０．１〜０．３mMとなるように用いるのが好ましい。
また、電子供与体としては、例えば１−メトキシ−５−メチルフェナジニウムメチルサルフェート（同仁化学研究所社製、1-methoxy ＰＭＳ）等を使用することができる。これらは、使用終濃度が０．００１〜０．０２ｍＭ、特に０．００２〜０．００５ｍＭとなるように用いるのが好ましい。
As a coloring reagent, a redox indicator and an electron donor are used. The redox indicator is preferably a tetrazolium salt which forms a color by being reduced and in which formazan formed is water-soluble, for example, 2- (4- iodophenyl ) -3- (4-nitrophenyl) -5- (2,4-Disulfophenyl) -2H-tetrazolium sodium salt (WST-1 manufactured by Dojindo Laboratories) and the like can be suitably used. It is preferable to use these tetrazolium salts so that the final concentration used is 0.05 to 0.5 mM, particularly 0.1 to 0.3 mM.
Further, as the electron donor, for example, 1-methoxy-5-methylphenazinium methyl sulfate (1-methoxy PMS, manufactured by Dojindo Laboratories) can be used. These are preferably used so that the final concentration for use is 0.001 to 0.02 mM, particularly 0.002 to 0.005 mM.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000143032A JP4438182B2 (en) | 2000-05-16 | 2000-05-16 | Drug sensitivity test plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000143032A JP4438182B2 (en) | 2000-05-16 | 2000-05-16 | Drug sensitivity test plate |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2001321156A JP2001321156A (en) | 2001-11-20 |
| JP2001321156A5 true JP2001321156A5 (en) | 2007-03-08 |
| JP4438182B2 JP4438182B2 (en) | 2010-03-24 |
Family
ID=18649903
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000143032A Expired - Lifetime JP4438182B2 (en) | 2000-05-16 | 2000-05-16 | Drug sensitivity test plate |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4438182B2 (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4526838B2 (en) * | 2004-03-04 | 2010-08-18 | エーザイ・アール・アンド・ディー・マネジメント株式会社 | Method for preparing sample for compoundability test and kit used therefor |
| JP2005341827A (en) * | 2004-06-01 | 2005-12-15 | Kyokuto Seiyaku Kogyo Kk | Plate for drug sensitivity test |
| JP2010213598A (en) * | 2009-03-16 | 2010-09-30 | Kyokuto Seiyaku Kogyo Kk | Method for examining efficacy of antimicrobial agent to microorganism |
| CN106248922A (en) * | 2016-07-22 | 2016-12-21 | 嘉兴鼎诺生物科技有限公司 | A kind of drug sensitive test of tumor cell detection plate and detection method |
| CN113845798A (en) * | 2021-09-15 | 2021-12-28 | 丹娜(天津)生物科技股份有限公司 | Antibacterial drug ink and method for preparing ester drug sensitive test strip by using same |
-
2000
- 2000-05-16 JP JP2000143032A patent/JP4438182B2/en not_active Expired - Lifetime
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