JP2001318091A - Member for blood serum or blood plasma separation and blood inspection container holding the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a member for blood plasma separation capable of conveniently holding a composition for blood serum or blood plasma separation into a container when necessary. SOLUTION: This member for blood serum or blood plasma separation is made up of a composition for blood serum or blood plasma separation and a storage member holding the composition, This member for separation is characterized in that the storage member is provided with a leak-out means for leaking out the composition when used.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a serum or plasma separation member used for separating serum or plasma by utilizing a difference in specific gravity of blood components, and a blood test container containing the same.

[0002]

2. Description of the Related Art Conventionally, in order to obtain serum or plasma necessary for a blood test, a blood test container in which a thixotropic serum or plasma separating composition is previously stored in the bottom of the container has been used ( For example, JP-A-51-83
654). Since the composition for separating serum or plasma has a specific gravity intermediate between serum and blood clot or between plasma and blood cells, when blood is collected in a container and centrifuged, the blood gradually rises from the bottom of the container and the serum layer and blood are separated. It moves to the middle of the mochi layer or between the plasma layer and the blood cell layer, forming a partition between the two layers. Due to the formation of the partition walls, the serum or plasma is separated, the collection of the serum or plasma is facilitated, and the effect that the separated state of the two layers is not damaged even when left for a long time is exhibited.

[0003] The serum or plasma separating composition has been distributed only in a form in which the composition is directly stored in the bottom of a blood test container in advance. This is because the serum or plasma separation composition is a gel-like fluid having thixotropy and is extremely low in handleability.For example, a user such as a nurse or a clinical laboratory technician can use this composition in a container at the inspection site. This is because the operation of filling the inside is extremely difficult.

[0004] However, in the field of clinical tests, there is also a demand for a technique that can utilize the composition as needed in a blood test. That is, for example, after blood was collected in a container not containing the composition in advance, when it was necessary to separate serum or plasma, blood was collected without transferring to another container containing the composition. This is a technique that allows the composition to be easily contained in a container.

However, conventionally, there has been no technique for filling a blood-collected container with a composition for separation later. Therefore, when the above-mentioned necessity arises, the composition is stored in advance from the blood-collected container. It has to be re-transferred to a container, which is not only complicated in operation, but also poses a problem that there is a risk of contact with blood when transferring.

Furthermore, in the conventional production of a blood test container directly containing a serum or plasma separating composition, there are various problems that cause a decrease in productivity.

For example, when filling a composition for separating serum or plasma into a container, great care must be taken to avoid the appearance of the composition from adhering to the inner wall surface of the container. .

In addition, various medicines may be applied to the inner wall surface of the container in accordance with the purpose of the blood test. In this case, if the composition adheres to the medicine, the function of the medicine cannot be exhibited. Therefore, the composition must be filled so as not to adhere to the drug.

Further, such a blood test container may be used as a so-called vacuum blood collection tube by reducing the pressure inside the blood test container. In this case, there is also a problem that the composition bumps and scatters in the container.

[0010]

DISCLOSURE OF THE INVENTION The present invention has been made to solve the above problems, and an object of the present invention is to easily and conveniently accommodate a serum or plasma separating composition in a container as required. It is an object of the present invention to provide a serum or plasma separation member and a blood test container containing the same, which solve the conventional problems in the filling step of the separation composition.

[0011]

According to the first aspect of the present invention,
A serum or plasma separation member comprising a serum or plasma separation composition and a storage member that stores the composition, wherein the storage member is provided with a leakage unit that leaks the composition during use. It is a member for separating serum or plasma.

[0012] According to a second aspect of the present invention, the leaking means comprises:
2. The serum or plasma separating member according to claim 1, wherein the member is a broken portion that is broken during use.

According to a third aspect of the present invention, the housing member is
2. A composition container comprising: a composition container; and a pressing member that presses the composition during use and causes the composition to leak out of the storage member.
It is a serum or plasma separation member of the above.

[0014] The invention according to claim 4 provides a composition comprising 25% of the above composition.
The serum or plasma separation member according to any one of claims 1 to ³ , which has a specific gravity at 1.0C of 1.03 to 1.08 g / cm3 and a viscosity at 25C of 50 to 1000 Pas. is there.

According to a fifth aspect of the present invention, there is provided a blood test container containing the serum or plasma separating member according to any one of the first to fourth aspects.

Hereinafter, the present invention will be described in detail.

The serum or plasma separating member of the present invention comprises a composition for separating serum or plasma and a housing member for housing the composition. In the following description, the “serum or plasma separation member” may be expressed as “separation member (of the present invention)” and the “serum or plasma separation composition” as “separation composition”.

The composition for separation in the present invention is generally called a serum separating agent or a plasma separating agent, and is a thixotropic gel-like composition. This composition moves between a clot and serum or blood cells and plasma by centrifugation after blood collection, and separates serum or plasma by forming a partition.

The composition for separation can be obtained, for example, by adding and mixing various additives to a synthetic resin having fluidity at ordinary temperature.

The above-mentioned composition for separation is particularly preferably composed of components comprising an oligomer of cyclopentadiene, a plasticizer and finely divided silica.

The oligomers of cyclopentadiene include oligomers obtained by multiplying cyclopentadiene,
And oligomers in which dicyclopentadiene has been multimerized in which cyclopentadiene has been dimerized. The oligomer can be produced by multiplying cyclopentadiene or dicyclopentadiene using, for example, a Diels-Alder reaction or the like. this is,
It is sometimes called dicyclopentadiene resin (DCPD resin). It is preferable that the oligomer is further hydrogenated to saturate the remaining double bond.

The above oligomer is JIS K6863
Those having a softening point of 70 to 140 ° C. measured by -1994 “Testing method of softening point of hot melt adhesive” are preferred. If the softening point is too low, phase separation is likely to occur, and if it is too high, melting becomes difficult and production becomes difficult.

The above oligomer is JIS K6862
-1984 “Melt viscosity test method for hot melt adhesives”
Those having a melt viscosity at 180 ° C. of 0.03 to 0.5 Pa · s as measured by the method A in the above are preferred. If the melt viscosity is too low, the viscosity of the present composition will be insufficient, and if it is too high, the viscosity of the present composition will be excessive.

The above-mentioned oligomer has a specific gravity at 25 ° C. (based on a floating / sedimentation test using a copper sulfate solution) of 1.02 to 1.02.
1.10 g / cm ³ , preferably 1.03 to 1.09
g / cm ³ are preferred. When the specific gravity deviates from the above range, it becomes difficult to suitably adjust the specific gravity of the present composition.

The above oligomer has a number average molecular weight of 200 to 800 in terms of polystyrene equivalent molecular weight distribution by the GPC method.
Are preferable, and more preferably 500 to 700.
The one having a weight average molecular weight of 500 to 1500 is preferable, and the weight average molecular weight is more preferably 700 to 1200.
If the number average molecular weight and the weight average molecular weight are too small, phase separation tends to occur, while if too large, the viscosity of the present composition becomes excessive.

The above oligomer preferably has a glass transition point of 40 to 90 ° C. according to the DSC method, more preferably 50 to 80 ° C. If the glass transition point is too small, phase separation tends to occur, and if it is too large, the viscosity of the composition becomes excessive.

The plasticizer is preferably a phthalate ester from the viewpoint of excellent compatibility with the cyclopentadiene oligomer. The phthalic acid ester is preferably one in which at least one of the alcohol residues forming two ester groups has 6 or more carbon atoms. Both diesters having 5 or less carbon atoms tend to have reduced compatibility with cyclopentadiene oligomers. Further, the carbon number of each of the alcohol residues forming the two ester groups is preferably 11 or less, since it is difficult to adjust the specific gravity of the present composition to a suitable range if it is too large.

Examples of the plasticizer include butylpentyl phthalate, dipentyl phthalate, butylhexyl phthalate,
Butyl heptyl phthalate, dihexyl phthalate, pentyl heptyl phthalate, butyl nonyl phthalate, pentyl octyl phthalate, hexyl heptyl phthalate, diheptyl phthalate, heptyl octyl phthalate, dioctyl phthalate, octyl nonyl phthalate, diisononyl phthalate, Examples include octyldecyl phthalate, diisodecyl phthalate, decylundecyl phthalate, diundecyl phthalate, and butylbenzyl phthalate. A particularly preferred plasticizer is a phthalic acid diester having 9 to 11 carbon atoms in each of the alcohol residues forming the two ester groups.

The amount of the plasticizer to be used is preferably 30 to 160 parts by weight, more preferably 50 to 150 parts by weight, and still more preferably 60 to 120 parts by weight, based on 100 parts by weight of the cyclopentadiene oligomer. is there. If the ratio is outside the above range, it becomes difficult to suitably adjust the viscosity of the composition.

The finely divided silica is an amorphous silicon dioxide (chemical formula SiO ₂ .n ((CH ₃ ) ₂ Si) in which most of the hydroxyl groups on the surface of the primary particles of silicon dioxide are substituted with methyl groups.
O)) is preferred.
Since the above-mentioned fumed silica has a hydrophobic property, it is well dispersed in an oligomer of cyclopentadiene or a plasticizer, but is not dissolved in blood and does not cause hemolysis, so that components in red blood cells may be mixed into serum or plasma. And is preferably used.

The specific surface area of the finely divided silica is 10 to 1
000 m ² / g is preferred, more preferably 50 to 50
0 m ² / g. When the ratio deviates from the above range, it becomes difficult to adjust the thixotropic property of the composition to a suitable range.

The primary particle diameter of the finely divided silica is 1 to 10
It is preferably 0 μm, more preferably 5 to 50 μm. When the ratio deviates from the above range, it becomes difficult to adjust the thixotropic property of the composition to a suitable range.

The amount of the finely divided silica used is preferably 1 to 50 parts by weight, more preferably 2 to 20 parts by weight, and still more preferably 5 to 20 parts by weight, based on 100 parts by weight of the cyclopentadiene oligomer. And most preferably from 6 to 16 parts by weight. If the ratio is outside the above range, it becomes difficult to suitably adjust the specific gravity or thixotropic property of the present composition.

The composition for separating serum or plasma of the present invention includes:
If necessary, an organic gelling agent may be added as a component.

The organic gelling agent is preferably a condensate of sorbitol and an aromatic aldehyde, and examples thereof include dibenzylidene sorbitol, tribenzylidene sorbitol, and methyl-substituted dibenzylidene sorbitol. Since they do not have water absorption or water solubility, even when they come into contact with blood for a long time, the present composition does not become cloudy due to water absorption and has no side effects such as blood concentration. Among these, dibenzylidene sorbitol is particularly preferred from the viewpoint of exhibiting the thixotropic property of the present composition well.

The amount of the organic gelling agent used is 0.02 to 100 parts by weight of the cyclopentadiene oligomer.
It is preferably 3 parts by weight, more preferably 0.1 to 2 parts by weight. When the ratio deviates from the above range, it becomes difficult to suitably adjust the thixotropic property of the present composition.

[0037] If necessary, a dispersant of an organic gelling agent may be added to the above composition as a constituent.

The dispersant for the above-mentioned organic gelling agent is preferably HL of 1.0 to 9.0, more preferably 4.0 to 6.0.
It is selected from the group consisting of a polyoxyethylene polyoxypropylene block copolymer having a B value, 1-methyl-2-pyrrolidone and a mixture thereof.

If the HLB value of the above block copolymer is too small, the effect of dispersing the organic gelling agent becomes insufficient, and the thixotropic property of the present composition decreases. When the composition is too large, the composition lacks hydrophobicity, and may dissolve in blood at the time of use of the composition, lyse the blood, and the components in the red blood cells may be mixed into serum or plasma to give an incorrect test result.

The amount of the block copolymer is 0.1 to 100 parts by weight of the oligomer of cyclopentadiene.
It is preferably from 15 to 15 parts by weight, more preferably from 0.1 to 5 parts by weight. If the amount of the block copolymer is too small, the effect of dispersing the organic gelling agent becomes insufficient, and the thixotropic property of the present composition decreases. If the amount is too large, the compatibility of the cyclopentadiene with the oligomer decreases, and the thixotropic property of the present composition decreases.

The above-mentioned 1-methyl-2-pyrrolidone dissolves the organic gelling agent well, does not react with blood to cause hemolysis, and decomposes the composition by irradiation for the purpose of sterilization. It is preferably used because it does not have any.

The amount of 1-methyl-2-pyrrolidone used is preferably 0.05 to 5 parts by weight, more preferably 0.05 to 3 parts by weight, based on 100 parts by weight of the cyclopentadiene oligomer. If the amount of 1-methyl-2-pyrrolidone is too small, the dispersing effect of the organic gelling agent will be insufficient, and the thixotropic property of the present composition will decrease.
Too much will cause a hemolytic reaction.

If necessary, appropriate amounts of specific gravity adjusters, compatibilizers, viscosity reducers, etc. may be added to the above-mentioned composition for separation as necessary.

The specific gravity of the present composition composed of the above-mentioned constituents is preferably intermediate between the specific gravity of serum or plasma and that of blood clots or blood cells. 08 g / cm ³ is preferable, and more preferably 1.04 to 1.06 g / cm ³ .

The viscosity of the present composition can be determined by the usual centrifugation operation, by placing the present composition in the middle of the serum or plasma layer and the clot or blood cell layer, or in a blood test container such as a vacuum blood collection tube. From the viewpoint of ease of filling work, the pressure is preferably 50 to 1000 Pa · s at 25 ° C., and more preferably 60 to 500 Pa · s.

As the method for producing the composition for separating serum or plasma, a commonly used method can be used, for example, a method in which the above-mentioned components are kneaded under heating and reduced pressure for a certain period of time. .

In the separation member of the present invention, the above-mentioned separation composition is stored in a storage member, and the storage member is provided with a leaking means for leaking the separation composition when used.

In the housing member, when the separation member of the present invention is used, the separating composition is caused to flow out of the housing member and to be moved between the serum layer and the clot layer or between the plasma layer and the blood cell layer. The configuration is not limited to any configuration as long as the configuration can be performed. Here, when the separating member is used, blood for separating serum or plasma,
This means that the container containing the separation member of the present invention is centrifuged.

That is, the above-mentioned storage member is capable of holding the composition for separation having thixotropic properties and poor handling properties in a constant shape until use, and maintaining the composition for separation in an easy-to-handle state. In addition, any configuration may be used as long as the composition for separation can be leaked into blood at the time of use.

The structure of the storage member in the separation member of the present invention is as follows.

As a first mode of the housing member, there is a configuration in which the leakage means provided on the housing member is a break portion configured to be broken during use. The break portion is configured such that a part of the partition wall of the housing member that houses the composition for separation breaks during use, that is, during centrifugation.

For example, as a material constituting the housing member,
A material having a specific gravity different from the specific gravity of the composition for separation, which is a film or sheet material, can be easily broken such as a weak seal portion or a thin portion in a part of the partition wall of the housing member. A part is provided. In the housing member thus configured, a force from the inside of the member by the separating composition acts on the broken portion due to a difference in specific gravity during centrifugation, and as a result, the broken portion is broken, and the separating composition leaks. You.

From the relationship with the specific gravity of blood and the composition for separation, it is more preferable that the material of the housing member has a specific gravity of 1.
It is good to be composed of 1 g / cm ³ or more. When a material having such a specific gravity is used, during centrifugation, a downward force acts on the housing member having a specific gravity larger than that of blood, and a specific gravity intermediate between serum and blood clots or between plasma and blood cells is exerted. An upward force on the container acts on the separating composition. As a result of this difference in force acting on a rupture portion provided in the housing member and rupture, the separation composition can leak.

As a second mode of the storage member, there is a configuration in which the storage member includes a composition container and a pressing member that presses the separating composition during use and leaks out of the storage member. In this aspect, the composition for separation is contained in a composition container, and the pressing member is configured to be leaked out of the container by pressing the composition for separation during centrifugation. .

For example, a material having a specific gravity greater than the specific gravity of the composition for separation is used as a material constituting the composition container and the pressing member, and a through hole is provided in a part of the pressing member. . During the centrifugal separation, a downward force acts on the housing member configured as described above, and the pressing member functions to press the composition for separation. Since the pressing member is provided with the through-hole, as a result of the pressing action, the separating composition leaks into the blood through the through-hole. From the relationship with the specific gravity of blood and the composition for separation, a material constituting the composition container and the pressing member has a specific gravity of 1.1 g / cm ³ or more.

The present invention also includes a blood test container in which the above-mentioned separating member is accommodated in a liquid separating container.

The shape of the liquid separation container is not particularly limited, but it is preferable to use a bottomed tubular container.

As a material of the liquid separation tube, for example,
Thermoplastic resins such as polyethylene, polypropylene, polystyrene, polyethylene terephthalate, polymethyl methacrylate, and polyacrylonitrile; thermosetting resins such as unsaturated polyester resins, epoxy resins, and epoxy-acrylate resins; cellulose acetate, cellulose propionate, ethyl cellulose, and ethyl chitin Modified silicate glass such as soda-lime glass, phosphosilicate glass and borosilicate glass; glass such as quartz glass; and those containing these as main components.

The blood test container can be used as a so-called vacuum blood collection tube. In this case, a stopper for sealing the opening of the container is liquid-tight so that blood does not leak outside. It is preferably air-impermeable from the viewpoint of maintaining the degree of vacuum. As a material of such a plug, at least one kind of elastic body selected from natural rubber, synthetic rubber and thermoplastic elastomer is preferable.

Examples of the synthetic rubber include isoprene rubber, styrene butadiene rubber, butyl rubber, halogenated butyl rubber, acrylic rubber, urethane rubber, and silicone rubber. Examples of the thermoplastic elastomer include polystyrene-based thermoplastic elastomers, polyolefin-based thermoplastic elastomers, polyurethane-based thermoplastic elastomers, polyester-based thermoplastic elastomers, polyamide-based thermoplastic elastomers, natural-rubber-based thermoplastic elastomers, and fluororubber-based thermoplastic elastomers. Examples thereof include a plastic elastomer.

The above-mentioned blood test container may be provided according to the purpose.
A blood anticoagulant may be contained therein. The storage method is not particularly limited, but is generally applied on the inner wall surface or stacked on the upper surface of the separating agent and stored. Examples of the blood anticoagulant include sodium dipotassium ethylenediaminetetraacetate, sodium tripotassium ethylenediaminetetraacetate, sodium disodium ethylenediaminetetraacetate, sodium heparin, lithium heparin, sodium fluoride, and citric acid. You.

In addition, the blood test container may contain a blood coagulation promoter therein, depending on the purpose. The storage method is not particularly limited, but is generally applied on the inner wall surface or stacked on the upper surface of the separating agent and stored. As the blood coagulation promoter, at least one selected from a hydrolase, a blood coagulation factor, and an inorganic substance is used.

Examples of the hydrolase include serine proteases such as trypsin and snake venom thrombin-like enzyme, thiol proteases such as cathepsin B and ficin, and metal proteases such as kininase I. Examples of the blood coagulation factor include thrombin,
Examples include in vivo enzymes such as factor Xa, factor IXa, factor XIa, factor XIIa, prekallikrein, and high molecular weight kininogen, phospholipids and calcium. Examples of the inorganic substance include water-insoluble inorganic fine powders such as glass, silica, kaolin, celite, and bentonite.

[0064]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the separating member of the present invention will be described below with reference to the drawings.

First, the above-described first embodiment in which the leakage means provided on the housing member is a break portion configured to be broken during use will be described.

The material constituting the housing member is as follows:
A film or sheet made of polyethylene, polypropylene, soft vinyl chloride resin or the like having a specific gravity of g / cm ³ or more is used.

In producing the separating member, first, the above-mentioned film or sheet is cut into a rectangular shape having an appropriate size, two sheets of the same size are stacked, and three sides are heat-sealed to form a bag. . After filling the bag with an appropriate amount of the separating composition, the remaining one side of the opening is heat-sealed to obtain the separating member 1 of the present invention as shown in the plan view of FIG. 1 and the sectional view of FIG. .

At least one of the four sides that are heat-sealed in the above process must be a weakly sealed breakable part that is broken by centrifugation under general conditions. In order to function as such a broken portion, the degree of fusion or the width of fusion between the films or sheets is appropriately adjusted. Here, when a general film or sheet is used, a preferable fusion width is about 0.5 to 3 mm. The degree of fusion is appropriately adjusted depending on the type of the material and the type of sealing machine used for fusion.

Of course, the separating member is not limited to those shown in FIGS. 1 and 2, and one film or sheet is folded in two along the center line, and after one side is heat-sealed, the separation is performed. The composition for use is filled, and then the remaining one side is heat-sealed to form the separating member 2 as shown in the plan view of FIG. 3 and the cross-sectional view of FIG.

Further, the method of providing a weakly sealable rupture portion is not limited to only thermal fusion, and various types of fusion such as high-frequency fusion or an adhesive may be used as long as it functions as a rupture portion. Adhesion may be performed.

Next, the above-mentioned second embodiment in which the housing member is composed of the composition housing body and the pressing member will be described.

As the composition container, a bottomed cylindrical body 31 as shown in FIG. 5 is used, and as the pressing member, a disc-shaped body 32 having a through hole at the center as shown in FIG. 6 is used. These members are manufactured by injection molding a thermoplastic resin such as polyethylene or polypropylene having a specific gravity of 1.1 g / cm ³ or more.

The disk 32 has a size such that its edge can slide up and down on the inner peripheral surface of the cylindrical body 31 so as to function as a pressing member. In addition, if the through-holes provided in the disc-shaped body 32 are too small, the composition is difficult to be extruded, and if the through-holes are too large, there is a possibility that they leak out even during use. Is preferred.

In the production of the separating member, after the required amount of the separating composition is accommodated in the bottomed cylindrical body 31, the disc-shaped body 32 is put on the composition like a lid. The separating member 3 of the present invention as shown in FIG.

Further, as an embodiment of the blood test container of the present invention, one in which the above-mentioned separating member is accommodated in tubular containers 41 and 51 is mentioned. As the blood test container 4 shown in FIG. 8 using the second separation member, there is a blood test container 5 shown in FIG.

[0076]

Next, an embodiment of the present invention will be described. However, the present invention is not limited to only these examples.

[Preparation of Composition] 100 parts by weight of an oligomer of cyclopentadiene (ECR251, manufactured by Tonex), a phthalic acid diester (having an alcohol residue having an alkyl group having 9 to 11 carbon atoms, manufactured by Mitsubishi Gas Chemical Company, Inc.) PL
-200) 77 parts by weight, 0.4 parts by weight of 1-methyl-2-pyrrolidone (manufactured by BASF), 0.1 parts by weight of dibenzylidene sorbitol (manufactured by Shin Nippon Rika Co., Ltd., Geruru D),
7.6 parts by weight of fine powdered silica (specific surface area: 250 m ² / g, manufactured by Tokuyama Corporation, Leolosil DM30S) was added to 15 m
The composition was prepared by kneading at 110 ± 10 ° C. under a reduced pressure of mHg. The specific gravity of the composition is 1.05 g / 25 ° C.
cm ³ and a viscosity of 110 Pa · s at 25 ° C.
Met.

[Example 1] A polyethylene film having a thickness of 0.05 mm was cut into a rectangular shape of 15 x 30 mm.
Two sheets of this film are overlaid, and the three sides are fused at a width of 1.0 m.
m and heat-sealed. 1 g of the above composition was filled into the bag-shaped body made of this film from the opening side, and the opening side was heat-fused with a welding width of 1.0 mm while taking care not to allow air bubbles to enter. did.

Example 2 A bottomed cylindrical composition container made of polypropylene and a disc-shaped pressing member were formed by injection molding. The disc-shaped pressing member has a diameter of 8.8 mm, and the bottomed cylindrical composition container has an outer diameter of 9.5 mm, an inner diameter of 9.0 mm, and a height of 24 mm.
0.5mm thick and 2.0mm inside diameter at center
Are provided. The composition container was filled with 1 g of the above composition, and a pressing member was placed on the composition and brought into close contact therewith to obtain a separation member of the present invention.

[Evaluation of Performance] The separation member of the above example was accommodated in a polyethylene-terephthalate-bottomed tubular blood test container having an inner shape of 10 mm. Thereafter, 8 mL of volunteer blood was injected into the container, and centrifuged at 1300 G for 5 minutes. As a result, in both Examples 1 and 2, the composition completely leaked from the housing member and migrated between the serum and the clot, and the serum and the clot were well separated.

[0081]

As described above, the serum or plasma separating member of the present invention is constructed as described above, so that the composition for separating serum or plasma can be conveniently contained in a container as required. Also,
The serum or plasma separation member and the blood test container containing the same according to the present invention have no problems in the conventional process of filling the separation composition into the container.

[Brief description of the drawings]

FIG. 1 is a plan view showing a first embodiment of a separation member.

FIG. 2 is a sectional view showing a first embodiment of the separation member.

FIG. 3 is a plan view showing a first alternative embodiment of the separation member.

FIG. 4 is a sectional view showing a first alternative embodiment of the separation member.

FIG. 5 is a view showing a composition container in a second embodiment of the separation member.

FIG. 6 is a diagram illustrating a pressing member according to a second embodiment of the separating member.

FIG. 7 is a view showing a second embodiment of the separation member.

FIG. 8 is a view showing a blood test container containing the separation member according to the first embodiment;

FIG. 9 is a view showing a blood test container containing the separation member according to the second embodiment;

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Separation member of 1st aspect 2 ... Separation member of 1st another aspect 3 ... Separation member of 2nd aspect 31 ... Composition container 32 ... Pressing member 4 ... Blood test container 41 ... Tubular container 5 … Blood test container 51… tubular container

Claims (5)

[Claims] Claims: 1. A serum or plasma separation member comprising a serum or plasma separation composition and a storage member storing the composition, wherein the storage member leaks the composition when used. A serum or plasma separation member characterized by comprising: 2. The serum or plasma separating member according to claim 1, wherein said leaking means is a break portion which is broken during use. 3. The serum or plasma separating member according to claim 1, wherein said housing member comprises a composition housing body and a pressing member which presses said composition during use to leak out of said housing member. 4. The composition according to claim 1, wherein the specific gravity at 25.degree.
1.08 g / cm ³ and a viscosity at 25 ° C. of 50
The serum or plasma separating member according to any one of claims 1 to 3, wherein the pressure is from 1000 to 1000 Pa · s. 5. A blood test container containing the serum or plasma separating member according to claim 1.