JP2001309724A - Method for sterilization in mushroom culture - Google Patents

Abstract

PROBLEM TO BE SOLVED: To solve a problem that in a chemical application for controlling harmful microorganisms in a mushroom culture, the sterilization in a production chamber is an incomplete state due to enlargement of facilities, influence of medium, etc., an application unevenness wets the chamber with a chemical, raises humidity in the chamber and makes the chamber in an environment to vigorously breed various germs and the cost of the chemical and labor itself for spraying the chemical are terrible. SOLUTION: This method for sterilization in a mushroom culture is characterized by sterilizing a chamber for culturing a mushroom by using an ozone gas. Further the ozone gas is generated by a discharge type ozonizer, and an ozone gas concentration in the chamber is uniformized by using a blower, etc. The sterilization treatment may be automatized by generating an ozone gas in 1 ppm concentration for at least 7 hours only in an inoculation chamber.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a production facility used for producing mushrooms, and more particularly to a method for sterilizing an inoculation room in which a mushroom fungus is inoculated into a bottle or the like or a culture room.

[0002]

2. Description of the Related Art Conventionally, control of harmful bacteria has been an important matter in fungal mushroom culture, and as a control method, chemical spraying has been mainly performed. In other words, the general procedure for mushroom culture is to first steam-disinfect the bottle for containing the medium for inoculating the mushroom fungus, move the disinfected bottle into the inoculation room,
After natural cooling, the bacteria are inoculated into the medium in the bottle. afterwards,
They are moved to a breeding room, ie, a culture room, and are raised.

[0003] At that time, for example, various bacteria enter the inoculation room due to human entry and exit, so that the sterilized bottles and the culture medium are contaminated by the various bacteria. Therefore, the medicine has been sterilized by spraying.

[0004]

However, the mushroom production equipment has become larger, especially the inoculation room has become wider, and corn cob has often been used as a culture medium, and the dust has become a factor that increases the contamination of harmful bacteria. As a result, in the conventional spraying of medicines, indoor sterilization is incomplete.

[0005] In addition, when the medicine is sprayed, there is unevenness in spraying, and the sprayed room becomes wet with a water-soluble drug.
The inside of the cultivation room or the like becomes extremely high in humidity, which results in an environment in which the propagation of various germs is vigorous.

[0006] Further, the cost of the drug and the labor for spraying the drug itself are also great. In view of such circumstances, in the present invention, the sterilization of bacteria that adhere to mushroom production facilities such as an inoculation room or a culture room or float in the air in the room can be performed as simply and reliably as possible without using labor. It is going to go to.

[0007]

SUMMARY OF THE INVENTION The present invention seeks to use ozone gas to solve the above-mentioned problems. The reason is that there are the following advantages in addition to the adverse effects of the above-mentioned drug application. (1) Since ozone is a natural natural product, it is decomposed into harmless oxygen even if used excessively. (2) It is strong against microorganisms and does not produce resistant bacteria. (3) The reaction is limited to the surface and does not alter the inside. (4) The product is an oxide, has no new toxicity, and has a small impact on the environment. (5) It has deodorizing and bleaching effects simultaneously with sterilization. (6) The generation amount and concentration can be easily controlled, and handling is safe. (7) The service life of the device is long and maintenance is easy. Thus, it is a very suitable sterilizing means for mushroom culture.

[0008] Therefore, ozone gas generators include those mainly using an ultraviolet lamp and those of the discharge type obtained by discharging at a high voltage of several KV or more. Although any method may be used, the ultraviolet lamp type is advantageous in that the structure is relatively simple, but as a disadvantage, generally, a high concentration ozone gas cannot be obtained. In the present invention, since a sterilizing power is required, a discharge-type ozone gas generator capable of obtaining a high concentration is employed. The discharge type ozone gas generator referred to here is a so-called silent discharge type ozone generator.

Therefore, a discharge type ozone generator is installed in a mushroom production facility such as an inoculation room or a culture room to generate ozone gas, and the specific gravity of the generated ozone gas is 2.4 times as heavy as that of air. The inside of the room is sterilized by stirring the inside of the room by means of air blowing means and the like to uniform the ozone gas concentration in various places in the room.

[0010]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail assuming that the present invention is used in an inoculation room or a culture room. In the inoculation room, mushrooms are planted in the soil inside the container such as a bottle. The bottle is usually steam-sterilized outside the room before being brought into the inoculation room. However, it has been described above that bacteria are floating due to people coming in and out.

Therefore, in order to examine the sterilizing power of ozone gas, a discharge-type ozone gas generator is provided in the inoculation room or the cultivation room. And the number were compared as examples.

[0012]

Example 1 First, Tables 1 and 2 show the results of investigations on the number of falling fungi and the decrease rate of filamentous fungi and bacteria by changing the ozone gas concentration while keeping the ozone gas treatment time constant. As can be seen from the table, the ozone gas concentration is 1 to 10 p.
The reduction rate of 90% or more was obtained at any of pm and 1 to 3 ppm having a lower concentration. It is considered that the reason why the rate of decrease in the number of falling bacteria does not become zero is that a person enters the room to investigate the falling bacteria.

Example 1 was performed under the following conditions. A) Samples Three Petri dishes (90 mm x 15 mm) of the following fungi were prepared. Bacteria: cultured on a standard agar medium at 25 ° C. for 48 hours. Filamentous fungi: cultured on PDA medium at 25 ° C. for 72 hours.

A) Treatment conditions Ozone concentration transition: 1 to 10 ppm for 8 hours, 15 ° C.
80% (Table 1) Ozone concentration transition: 1 to 3 ppm for 8 hours, 15 ° C
80% (Table 2) In addition, the implementation place is a mushroom culture room, and the size is 6.8 m.
² (19 m ³ ).

C) Investigation method After each petri dish of the sample was opened in the culture chamber for 30 minutes,
After counting the number of falling bacteria in the air, the ozone concentration in the culture chamber was adjusted to the above-described processing conditions, and then the number of falling bacteria was counted.

Table 1 shows that the change in ozone concentration is 1 to 10 ppm.
After 8 hours of treatment, as can be seen, the sterilizing effect of ozone gas on airborne bacteria was excellent. The number of falling bacteria is an average of three times.

[0017]

[Table 1]

[0018]

[Table 2]

Table 2 shows the results after treatment for 8 hours at ozone concentration transitions of 1 to 3 ppm. As can also be seen from the table, it is clear that the ozone gas has an excellent sterilizing effect on airborne bacteria. You. In addition, the number of falling bacteria in Table 2 is also an average value of three times.

Next, as Example 2, only for filamentous fungi, Table 3 shows the results of examining the bactericidal effect for each treatment time while keeping the ozone concentration constant at 1 ppm in the culture chamber. Example 2 was performed under the following conditions. A) Samples Three Petri dishes (90 mm x 15 mm) of the following fungi were prepared. Filamentous fungi: cultured on PDA medium at 25 ° C. for 72 hours. That is, it is the same as the first embodiment.

A) Treatment conditions Ozone concentration transition: treatment at 1 ppm for 9 hours, 15 ° C., 70
% (Table 3) The site of the experiment was a mushroom culture room and the size was 6.8 m.
² (19 m ³ ), which is the same place as in the first embodiment.

C) Investigation method After the sample dish was opened in the culture chamber for 30 minutes, the number of bacteria falling in the air was counted, and the ozone concentration in the culture chamber was reduced to 1 p.
pm, and the number of falling bacteria was counted at each time. 1 hour treatment, 89% filamentous fungi, 100% after 3 hours
Decreased.

[0023]

[Table 3]

Further, Table 3 shows the results of Example 3 performed in the inoculation room whose location was changed under the same conditions as in Example 2. Example 3 was performed under the following conditions. A) Samples Five Petri dishes (90 mm x 15 mm) of the following fungi were prepared. Bacteria: cultured on a standard agar medium at 25 ° C. for 48 hours. Filamentous fungi: cultured on PDA medium at 25 ° C. for 72 hours. That is, the number of petri dishes is different, but the same as in the first embodiment.

A) Treatment conditions Ozone concentration transition: treatment at 1 ppm for 7 hours, 7 ° C., 60%
(Table 4) In addition, the implementation place is mushroom inoculation room, size 64.8m ²
(246 m ³ ), which is much larger than the culture room.

C) Investigation method After the sample dish was opened in the inoculation room for 30 minutes, the number of bacteria falling in the air was counted, and the ozone concentration in the inoculation room was reduced to 1 p.
pm, and the number of falling bacteria was counted at each time.

[0027]

[Table 4]

In this case, a sudden increase in falling bacteria is observed after 3 hours. This is caused by the fact that the bacteria were brought in by a person coming and going by accidental replacement of the ozone gas generator immediately before conducting the investigation after 3 hours. I think that the. However, after 7 hours, the reduction rate of the filamentous fungi was also 80%.

In the present invention, as can be seen from Example 1, when the ozone gas treatment time is fixed and the ozone gas concentration is changed from 1 to 10 ppm, the decrease rate of the number of falling fungi and bacteria is as follows. It could be confirmed that it became 90% or more. Further, from Examples 2 and 3, it was confirmed that the filamentous fungi decreased by 100% after 3 hours in the culture room, and the filamentous fungi decreased by 80% after 7 hours even in the large inoculation room. .

Based on these facts, various kinds of bacteria adhering to or floating in the air in a mushroom production facility such as an inoculation room or a culture room can be treated with an ozone gas concentration of 1 ppm for 7 hours or more. It was confirmed that the plant could be purified to an environment sufficient for growing it.

However, since ozone gas has an effect on the human body, ozone gas is generated during nights when no people are present.
You should not enter the room until: Therefore, the operation of the ozone gas generator may be controlled by a timer or the like.

It should be noted that at about 0.1 ppm, sterilization cannot be performed because of deodorization. Also, if mushrooms grow to some extent,
The installation of the ozone gas generator may be limited to a place such as an inoculating room or the like where there is no growing culture medium and mushrooms, since normal harmful fungi will not be damaged.

[0033]

As described above, since ozone gas itself is a natural product, it has many advantages as described above.
Suitable for sterilization of mushroom culture, ozone concentration 1ppm
If the treatment is carried out for more than 7 hours, there are many advantages such as less spray unevenness than the chemical spray, no wetting in the spray room, low running cost only with electricity bill, stable and high sterilization effect. have. Of course, the generation of ozone gas can be easily automated with a timer or the like, so that labor for spraying such as chemical spraying is not required, and this is a truly useful invention in the mushroom industry.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Shigeyuki Tsunoda 2206 Osuro, Matsushiro-cho, Nagano City, Nagano Prefecture F-term (reference) 2B011 CA19 GA06 PA01

Claims (3)

[Claims] 1. A method for controlling harmful bacteria in mushroom production, wherein a room for cultivating mushrooms is sterilized by using ozone gas. 2. The sterilization during mushroom cultivation according to claim 1, wherein the ozone gas is generated by using a discharge type ozone gas generator and the gas is diffused by using a blower or the like to make the concentration of ozone gas in the room uniform. Method. 3. The method according to claim 1, wherein the ozone gas is generated at a concentration of 1 ppm for more than at least 7 hours, and the indoor ozone gas treatment time is automated by a timer or the like. Sterilization method.