JP2001305433A - Microscope device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a microscope device capable of performing an observing work in accordance with observation conditions, and also, capable of easily switching the observation conditions. SOLUTION: As for the microscope body 1 with a visible light optical system for performing a visual observation by a lens barrel binocular part 8 and an ultraviolet light optical system for observing the image picked up by an ultraviolet light observation TV camera 15 on a monitor 54 of a computer 53, by clicking 'REMOTO ON+UV' or 'REMOTO OFF+BF' displayed on the control panel of the monitor 54, the visual observation by the binocular part 8 is switched to the observation of the image picked up by the ultraviolet light observation TV camera 15 on the monitor 54, and vice versa.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a microscope apparatus provided with a magnifying optical system for visual observation using a microscope observing barrel and an enlarging optical system for observing electronic images.

[0002]

2. Description of the Related Art In recent years, microscope systems have become diversified in the configuration of optical systems in response to demands for higher magnifications and higher resolutions, and are commonly used with disk scan type confocal optical systems and ultraviolet optical systems. A combination of the above optical systems for visible light observation has been put to practical use.

Japanese Patent Application Laid-Open No. H11-52253 shows an example of a microscope apparatus based on such a concept. By switching illumination between a visible light region and an ultraviolet light region, an image observed by a visible light optical system can be changed. An image observed by an observation lens barrel and an ultraviolet light optical system can be observed by a TV camera (and a display device). In this case, in a high-magnification optical system, observation with the naked eye may not be possible due to the wavelength range or image processing used in combination. In such a case, an image captured by an image sensor such as a TV camera may be displayed on a TV monitor or a personal computer. The image is displayed on the upper monitor for observation.

Japanese Patent Application Laid-Open No. 5-127096 discloses that although an optical system including an objective lens is common, an image forming optical system is branched and a near-ultraviolet image and a color image in the visible wavelength range are formed. A display for simultaneous or switching display is disclosed.

Further, Japanese Patent Application Laid-Open No. 11-40629 discloses that a confocal optical system and a non-confocal optical system (a normal visible microscope) can be simultaneously displayed on a single monitor. An effect that an image can be monitored and monitored simultaneously is disclosed.

[0006]

SUMMARY OF THE INVENTION However, Japanese Patent Application Laid-Open
In the system disclosed in JP-A-52253, in which the visible light optical system and the ultraviolet light optical system can be switched, first, the visible light optical system is selected, and the naked eye is observed from low magnification to high magnification by the observation lens barrel. Then, after determining the observation range, the system is switched to an ultraviolet optical system, and a high-resolution, high-resolution image is observed on a TV monitor or a monitor on a personal computer. A problem arises in that a troublesome operation is required for switching of the illumination system and various optical elements accompanying the switching of.

Further, as disclosed in JP-A-5-127096 and JP-A-11-40629,
If a microscope image by a normal visible light optical system is also displayed on the monitor, the number of pixels of the image sensor of the TV camera,
There is a problem that the resolution, the visual field, the brightness, and the like are inferior to the naked eye observation image from the eyepiece due to the size, sensitivity, and the like. By the way, usually, the visual field of the visual observation image is from 20 mm to
The image sensor is typically 10 m
m, and the observation field of view becomes narrow. Further, since the number of pixels of the image sensor is about several hundred thousand to several million, it is far inferior to the resolution of human eyes. Further, the sensitivity and dynamic range regarding the brightness of the image sensor are inferior to those of the human eye. Further, in the above-mentioned conventional example, there is a case where two different images are displayed on one monitor. However, the monitor size cannot be used effectively, and as a result, the display screen becomes small. Therefore, with respect to visible light observation, observation with the naked eye can provide the highest quality observation image.

On the other hand, in recent years, there has been an apparatus in which the switching operation of the illumination system and various optical elements when the optical system is switched is performed electrically, and the complexity of the control at this time is reduced, so that the image display and the microscope can be performed. Others used computers to facilitate control.

In Japanese Patent Application Laid-Open No. Hei 7-199077, a main control unit for controlling a computer via an interface circuit is connected to a unit control unit for switching an observation system and a photographing system unit of a microscope. In this main control unit, a device is disclosed in which an operation screen for displaying the operation of each unit is operated by inputting with a keyboard or a mouse to control the corresponding unit.

[0010] However, in the case of using such a computer, it is convenient to perform complicated control and operation switching, but these input operations must be performed on the operation screen using a keyboard, a mouse, or the like. Therefore, for example, as described above, in order to obtain a high-quality observation image with a visible observation system, a spectacle operator performs various operations while actually looking into the microscope, such as a visible light microscope observation based on visual observation. What you do is not as effective. That is, in the visible light microscope observation based on the naked eye observation, focusing (focusing), switching of the objective lens, switching of the microscopy method, etc. are performed while operating a controller near the microscope or an operation switch built in the microscope body. The brightness and the aperture are adjusted in the observation method, but these are designed so that they can be operated while looking through the microscope. The use of a keyboard, a mouse, or the like has a problem in that the efficiency of observation work is rather reduced.

The present invention has been made in view of the above circumstances, and it is an object of the present invention to provide a microscope apparatus capable of performing stable observation suitable for observation conditions and easily switching these observation conditions. .

[0012]

According to the first aspect of the present invention,
A microscope body having a magnifying optical system for visual observation and a magnifying optical system for performing electronic image observation by a microscope observation barrel, and an operation image for operating the microscope, and a microscope for the microscope body by operating on the operation image It is characterized by comprising a control unit for controlling switching between a visual observation magnifying optical system using an observation lens barrel and a magnifying optical system for performing the electronic image observation.

According to a second aspect of the present invention, in the first aspect of the present invention, the microscope main body can be switched on the operation screen by a login signal from the control means. A switching operation to a magnifying optical system for performing login and the electronic image observation by one operation on the operation image, and a switching operation to a macroscopic observing magnifying optical system by a logoff and a microscope observation barrel unit. Features.

As a result, according to the present invention, it is possible to switch between the macroscopic observation and the electronic image observation by the microscope observation lens barrel according to the observation conditions, so that various operations can be performed in a posture suitable for each observation condition. It can be carried out.

Further, since only one operation on the operation image is required for switching between the magnifying optical system for performing electronic image observation and the magnifying optical system for visual observation using the microscope observation lens barrel, these operations are switched. Operation is easy.

[0016]

Embodiments of the present invention will be described below with reference to the drawings.

(First Embodiment) FIG. 1 shows a schematic configuration of a microscope apparatus to which the present invention is applied. In this case, the microscope apparatus has a visible light optical system based on epi-illumination and an ultraviolet light optical system based on ultraviolet illumination.

In FIG. 1, reference numeral 1 denotes a microscope main body, on which a revolver 2 is arranged corresponding to a stage 21. This revolver 2 is provided with five objective lenses 3 as shown in FIG.
Reference numeral 4 denotes an objective lens 3 for visible light, and # 5 an objective lens 3 for ultraviolet light. The objective lens 3 of # 1 is 1
0 × magnification, # 2 objective lens 3 is 20 × magnification, # 3
The objective lens 3 has a magnification of 50 ×, the objective lens 3 of # 4 has a magnification of 100 ×, and the objective lens 3 of # 5 has a magnification of 150 × (or 100 ×). Is used.

The revolver 2 is rotatable so that the desired objective lens 3 can be selectively switched on the optical axis 4 by the rotation of the motor M1. further,
A sensor S1 is arranged near the revolver 2 so that the type of the object can be recognized from the hole position of the revolver 2.

The stage 21 mounts the sample 29 and is horizontally movable in the X and Y directions so that the objective lens 3 can adjust the observation center position of the sample 29 with respect to the optical axis. The stage 21 can be driven in the vertical direction by a motor M2, so that the focus position of the sample 29 on the stage 21 can be adjusted.

The motors M1 and M2 are driven by a drive circuit 22 to which a command from a control circuit 23 is given, and a signal corresponding to the recognition result of the sensor S1 is fed back to the control circuit 23.

An operation unit 24 is connected to the control circuit 23. The operation unit 24 includes an objective selection switch 31 to 35 corresponding to each of the objective lenses # 1 to # 5, an encoder dial 36 that outputs a pulse for moving the stage 21 up and down according to a rotation operation, and an illumination described later. Light source 5
Has a light control dial 101 that controls the light. In this case, since the objective selection switch 35 is compatible with the # 5 ultraviolet light objective lens 3, it is assumed that the operation cannot be performed.

The microscope body 1 has a visible light optical system and an ultraviolet light optical system. Among these, in the visible light optical system, light from the illumination light source 5 for visible observation is reflected by the half mirror 25 (having the characteristic of transmitting ultraviolet light) through the illumination system lens 6, and the objective lens 3 for visible light is used. Irradiates the sample 29 through the objective lens 3, and the reflected light from the sample 29 is enlarged by the objective lens 3, transmitted through the half mirror 25, and has a characteristic of transmitting a dichroic mirror 26 (transmitting visible light and reflecting ultraviolet light). ), An enlarged image is formed at the image position 9 via the imaging lens 7, and can be visually observed by the binocular unit 8 of the lens barrel. In this case, a light-shielding member 10 is arranged on the front surface of the illumination light source 5, and the light-shielding member 10 is driven by a motor M <b> 3 to open the light from the illumination light source 5 for visible observation and to block the light for ultraviolet light observation. Like that. The motor M3 is
It is driven by a drive circuit 22 to which a command from a control circuit 23 is given. The supply voltage of the illumination light source 5 is adjusted via the control circuit 23 by operating the light control dial 101 of the operation unit 24, and the light is controlled.

On the other hand, the ultraviolet imaging optical system transmits light from the illumination light source 11 for observing ultraviolet light to the illumination system lens 12 and the light reduction unit 10.
The light is reflected by an ultraviolet light half mirror 41 via the mirror 2, further reflected by the ultraviolet light mirror 42 and the dichroic mirror 26, and irradiated on the sample 29 via the half mirror 25 and the ultraviolet light objective lens 3. The reflected light from 29 is enlarged by the objective lens 3 and transmitted through the half mirror 25,
The light is reflected by the dichroic mirror 26 to form the imaging lens 1
4. Ultraviolet light mirror 42, Ultraviolet light half mirror 41,
An image is formed at an imaging position 16 of the ultraviolet light observation TV camera 15 via the imaging lens 14. In this case, a light-shielding member 13 is also provided on the front surface of the illumination light source 11, and the light-shielding member 13 is driven by a motor M <b> 4 to open the light from the illumination light source 11 at the time of ultraviolet light observation and to block the light at the time of visible light observation. I am trying to do it. The motor M4 is driven by the drive circuit 22 to which a command from the control circuit 23 is given. Further, the dimming unit 102 is a combination of a plurality of dimming filters (in the drawing, three types of dimming filters IN-OU).
T (six combinations by T) allows the light amount of the illumination light source 11 to be adjusted stepwise.

The control circuit 23 has, for example, a maximum magnification of 1
The focus position by the 00 × visible light objective lens 3;
A storage unit 23a for setting the difference between the in-focus positions of the 0 × ultraviolet light objective lens 3 as a focus correction value;
When the objective lens 3 for visible light is switched from the objective lens 3 for visible light to the objective lens 3 for 150 × ultraviolet light, the focusing position of the objective lens 3 for ultraviolet light is corrected based on the parallax correction value set in the storage means 23a. It has a parfocal correction function.

On the other hand, the TV camera 15 for ultraviolet light observation has
Capture board 52 via camera controller 51
The capture board 52 includes a monitor 5
4 is connected (actually, the capture board 52 is built into the computer 53 or is in a slot of the computer 53). The capture board 52 has an interface function for displaying an image on a monitor 54 via a computer 53.

The computer 53 has a cable 5
The control circuit 23 is connected to the control circuit 23 via the control circuit 5. in this case,
The control circuit 23 has a built-in interface circuit for the computer 53. This interface circuit is a general one, such as RS232C or GPIB. When the control circuit 23 receives a “log-in” signal from the computer 53 via the cable 55, the control circuit 23 does not accept an input from the operation unit 24 (mask processing). 23 is controlled, and the motors M1 to M4
Is operated. At this time, switching and interlocking of various optical elements by the control circuit 23 are equivalent to signals from the operation unit 24.

FIG. 3 shows the structure of the software 56 in the computer 53, which has an image input unit 61, a resource management unit 62, an image display unit 63, and a microscope control unit 64. The image input unit 61 receives an image signal input from the ultraviolet light observation TV camera 15 into the capture board 52. The resource management unit 62
The image display unit 63 determines whether or not the image input according to the instruction from the microscope control unit 64 is to be displayed on the monitor 54.
To instruct. The image display unit 63 is connected to an image window 65 of the monitor 54, and the microscope control unit 64 is connected to the control circuit 23 and the control panel 66 of the monitor 54. The image window 65 displays an image captured by the ultraviolet light observation TV camera 15 according to an instruction from the image display unit 63. The control panel 66 displays an operation image or the like for operating the microscope according to an instruction from the microscope control unit 64, and, by clicking a button on the display screen with a mouse, sends an operation command to the control circuit 23 via the microscope control unit 64. Output to

FIGS. 4A and 4B show a display example concretely displayed on the monitor 54. FIG. 4A shows a log-off in which the computer 53 is not communicating with the microscope main body 1. FIG. 2B shows a login state in which the computer 53 is communicating with the microscope main body 1. In the image window 65, a captured image of the ultraviolet light observation TV camera 15 is displayed in a window format. In addition, the control panel 66, which is an operation image for operating the microscope, has a “REMOTE ON + UV” button 66a, # in the log-off state shown in FIG.
Objective selection buttons 6 corresponding to the respective objective lenses 3 of # 1 to # 5
6b, a focus up / down button 66c for moving the stage 21 up and down, and a brightness adjustment button 66d for stepwise switching the brightness during ultraviolet light observation are displayed. Here, "R
When the “EMOTE ON + UV” button 66 a is clicked on with a mouse, the microscope main body 1 is set to “login state” (PC remote control) via the interface circuit of the control circuit 23, and the objective lens is controlled by the control circuit 23 and the drive circuit 22. Switch to the # 5 objective lens 3 for ultraviolet light, and further switch the illumination and observation optical system to ultraviolet light, and display the TV camera 1 for ultraviolet light observation on the image window 65.
5, an ultraviolet light observation image picked up is displayed.

FIG. 4B shows a display example on the control panel 66 in such a login state.
The image window 65 includes a TV camera 15 for observing ultraviolet light.
Is displayed, and the control panel 66 displays “REMOTE ON
+ UV ”button 66a instead of“ REMOTE OF
An "F + BF" button 66e is displayed. In addition to the objective selection button 66b, the focus up / down button 66c, and the brightness adjustment button 66d, an indicator notifying that the # 3 objective lens 3 for ultraviolet light has entered the optical axis. 66f is displayed.

Next, the operation of the embodiment configured as described above will be described.

First, the examiner operates the objective selection switch 31 or 32 of the operation unit 24. Then, Revolver 2
Is driven to rotate, and the low-magnification objective lens 3 for visible light is
Located on top. Then, in this state, the focus position on the sample 29 is adjusted, and the observation position on the sample 29 is determined by moving the stage 21 in the XY directions. As the illumination and optical system in this case, an illumination light source 5 for visible light observation and a visible light optical system are selected, and an observation image from the sample 29 is visually observed with the lens barrel binocular unit 8. That is, in this state, the computer 53 is not communicating with the microscope main body 1 and
At this time, a visible light optical system is selected as the optical system, and the illumination light source 5 is used.
Is reflected by the half mirror 25 via the illumination system lens 6 and irradiates the sample 29 via the visible light objective lens 3, and the reflected light from the sample 29 is enlarged by the objective lens 3, An enlarged image is transmitted through the mirror 25, is formed on the image position 9 via the dichroic mirror 26, and the imaging lens 7, and is visually observed by the binocular unit 8 of the lens barrel.

In this case, when, for example, a 10 × visible light objective lens 3 is selected as the low magnification objective lens 3 by the objective selection switch 31, the magnification is not high and there is a certain depth of focus. Because of the fact that the working distance is long, the focusing operation on the sample 29 and the adjustment of the observation position can be easily performed.

Thereafter, the other objective selection switches 32, 3
3 and 34 are operated to select and switch each of the 20 ×, 50 ×, and 100 × visible light objective lenses 3. The adjustment of the focus position and the observation position with these objective lenses 3 is performed for the visible light. Since the objective lens 3 may be sequentially switched to a high magnification and finely adjusted, the focus position and the observation position on the sample 29 can be easily obtained, and the visible light observation can be performed while adjusting the brightness of the illumination at each magnification. Can be performed.

Next, when a higher-resolution and higher-resolution observation is performed, the observation is performed by an ultraviolet optical system, and the lens barrel binocular unit 8 is used.
The spectator goes to the computer 53 because visual observation cannot be performed. In this case, the monitor 5 of the computer 53
4 is in a log-off state shown in FIG. 4A in which the computer 53 is not communicating with the microscope main body 1.

In this state, at step 501 shown in the flowchart of FIG. 5A, the speculum examiner selects the “REMOTE ON + UV” button 6 on the control panel 66.
When mouse 6a is clicked on with a mouse, in step 502, the microscope main body 1 enters a "logged-in state" (PC remote control) via the interface circuit of control circuit 23, and in step 503, the illumination system and the optical system change the ultraviolet light. The illumination light source 11 for observation and the ultraviolet light optical system are switched. In this case, the revolver 2 is rotationally driven by the control circuit 23 and the drive circuit 22 to generate
The # 5 objective lens 3 for ultraviolet light is positioned on the optical axis 4, the light shielding member 10 blocks the light from the illumination light source 5, and at the same time, the light shielding member 13 retreats from the optical path,
The illumination light source 11 and the ultraviolet light optical system are selected, and the observation image from the sample 29 is captured by the ultraviolet light observation TV camera 15,
The data is taken into the computer 53 via the camera controller 51. Thus, an ultraviolet light observation image 65a captured by the ultraviolet light observation TV camera 15 is displayed in the image window 65 of the monitor 54, as shown in FIG. 4B. The control panel 6 in this login state
6 is “REMOTE ON +” as shown in FIG.
"REMOTEOFF +" instead of "UV" button 66a
BF "button 66e is displayed, and # 5 for ultraviolet light is displayed.
An indicator 66f that indicates that the objective lens 3 has entered the optical axis is displayed.

In this state, the examiner operates the image window 6
By clicking the focus up / down button 66c and the brightness adjustment button 66d on the control panel 66 with the mouse while observing the ultraviolet light observation image 65a displayed on the screen 5, the focus position and the observation position on the sample 29 are displayed. And ultraviolet light observation can be performed while appropriately adjusting the brightness of the illumination.

In this case, the observer observes the ultraviolet light observation image 65a in the image window 65 on the same monitor 54, and presses the focusing up / down button 66 on the control panel 66.
Since c and the brightness adjustment button 66d can be operated, observation can be performed in a stable state without taking an unreasonable posture for ultraviolet light observation. Further, in such an ultraviolet light observation, it is known that the depth becomes shallow in addition to the high magnification and the high resolution, but the focusing operation for this is performed by the focusing up / down button 66 c on the control panel 66. By doing so, it is possible to operate at a fine pitch, and, for example, one
Fine adjustment such as sending 0.01 μm with each click can also be performed.

Thereafter, the observation of the ultraviolet light is terminated,
In order to return to the visible light observation, the examiner clicks the “REMOTE OFF + BF” button 66e of the control panel 66 with the mouse in step 504 shown in the flowchart of FIG. 5B. Then, in step 505, the microscope main body 1 enters the “log-off state” (PC local control), and step 506 is performed.
Then, the illumination system and the optical system are switched to the illumination light source 5 for visible light observation and the visible light optical system. in this case,
The revolver 2 is rotationally driven by the control circuit 23 and the drive circuit 22 so that the objective lenses 3 for visible light # 1 to # 4 are positioned on the optical axis 4 and the light shielding member 13 is
And at the same time, the light blocking member 10 is retracted from the optical path, and the illumination light source 5 and the visible light optical system are selected.
The observation image from the sample 29 can be visually observed with the lens barrel binocular unit 8. In the case of switching to such visible light observation, the objective lens 3 positioned on the optical axis 4 has the lowest magnification or the highest magnification depending on the application, but in this embodiment, FIG. As shown in (), the objective 66 is switched to the visible light objective lens 3 having the maximum magnification of 100 × and displaying the indicator 66g.

In this state, the examiner operates the computer 53
The operation for ultraviolet light observation toward is ended, and the user again goes to the front of the microscope main body 1 to perform visible light observation, and performs normal microscope observation using the operation unit 24 while looking into the lens barrel binocular unit 8. .

In the control panel 66 of the monitor 54 in this log-off state, a "REMOTE ON + UV" button 66a is displayed again instead of the "REMOTE OFF + BF" button 66e as shown in FIG.

Accordingly, in this way, the speculum can display “RE” displayed on the control panel 66 of the monitor 54.
"MOTE ON + UV" button 66a or "REMO
By simply clicking the “TEOFF + BF” button 66e, it is possible to switch the observation of the ultraviolet light observation image 65a by the image window 65 or the naked-eye observation while looking through the binocular section 8 of the lens. Can be performed, and stable observation can be performed.

Further, the only operation required for switching these observation conditions is a single operation of clicking the "REMOTE ON + UV" button 66a or the "REMOTE OFF + BF" button 66e alternately displayed on the control panel 66 of the monitor 54. These switching operations can be simplified.

Further, a special function other than the normal operation such as a fine focusing operation can be added to the control panel 66 of the monitor 54.

(Second Embodiment) FIG. 6 shows a schematic configuration of a second embodiment of the present invention, and the same parts as those in FIG. 1 are denoted by the same reference numerals. Further, the software shown in FIG. 3 and the monitor image shown in FIG. 4 are the same as those in the second embodiment, so that FIG.

In this case, the visible light optical system includes a lens barrel binocular unit 8.
A TV camera 111 for observing visible light attached to the three-lens port of
The beam splitter 112 is inserted between them. The beam splitter 112 divides a certain ratio of the light amount of the observation image of the sample 29 into the lens barrel binocular unit 8 and divides the remaining ratio into the visible light observation TV camera 111. A camera controller 51 a for sending a captured image to the capture board 52 is connected to the visible light observation TV camera 111. The software resource management unit 62 shown in FIG. 3 selects which of the images obtained from the visible light observation TV camera 111 and the ultraviolet light observation TV camera 15 to display according to the state instruction of the microscope control unit 64. The observation image of the sample 29 at the time of visible light observation can be observed by both the lens barrel binocular unit 8 and the monitor 54.

In such a configuration, the display screen of the monitor 54 of the computer 53 is assumed to be in the log-off state shown in FIG. "REMOTE
Click on the "ON + UV" button 66a with the mouse.
When the microscope body 1 enters the “logged-in state” (PC remote control), the illumination system and the optical system are switched to the illumination light source 11 and the ultraviolet optical system for observing the ultraviolet light. Various operations can be performed by the focus up / down button 66c and the brightness adjustment button 66d.

When the user wants to end such ultraviolet light observation and return to visible light observation, he / she can use “REMOTE” on the control panel 66 in the login state shown in FIG. 4B.
When the "OFF + BF" button 66e is clicked on with a mouse, the microscope body 1 enters the "log-off state" (PC local control), and the illumination system and the optical system are changed to the illumination light source 5 and the visible light optical system for visible light observation. Is switched. Thereby, the speculum can perform a normal microscope observation by operating the operation unit 24 while looking into the lens tube binocular unit 8.
The observation image of No. 9 is taken by the visible light observation TV camera 111 and is displayed on the image window 65 of the monitor 54 as a visible light observation image. Can be observed. When observing the visible light while in the logged-in state, click and switch the objective lens button for visible light of the objective lens button 66b, and move the focus up / down button 66c and the brightness adjustment button 66d on the control panel 66 with the mouse. By clicking with, the in-focus position and the observation position on the sample 29 are obtained, and the visible light observation can be performed while appropriately adjusting the brightness of the illumination.

Accordingly, in this case, the user can log off the microscope main body 1 when operating the operation section 24 in the visible light observation, or on the control panel 66 of the monitor 54 when operating the computer 53 side. Can be performed with one touch (press one button). This also means
Even when observing with visible light, if the resolution is not so high because of the positioning on the sample, all operations can be performed on the control panel 66 of the monitor 54 while the microscope main body 1 is logged in. It is possible to widen the range of choices of the operation of the microscopic person, and it is possible to improve the usability as the microscope apparatus.

The present invention is not limited to the above-described embodiment, but can be variously modified without departing from the essence of the invention. For example, a confocal optical system is combined with an ultraviolet optical system. It is also possible. Further, the present invention is not limited to the ultraviolet light optical system, but is also applicable to a confocal observation optical system dedicated to electronic images that cannot be observed with the naked eye, such as image processing type confocal observation using visible light.

[0051]

As described above, according to the present invention, it is possible to provide a microscope apparatus which can perform stable observation suitable for observation conditions and can easily switch observation conditions.

[Brief description of the drawings]

FIG. 1 is a diagram showing a schematic configuration of a first embodiment of the present invention.

FIG. 2 is a diagram showing a schematic configuration of a revolver used in the first embodiment.

FIG. 3 is a diagram showing a schematic configuration of software of a computer used in the first embodiment.

FIG. 4 is an exemplary diagram showing a display example of a monitor image according to the first embodiment;

FIG. 5 is a flowchart showing the operation of the first embodiment.

FIG. 6 is a diagram showing a schematic configuration of a second embodiment of the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 ... Microscope main body 2 ... Revolver 3 ... Objective lens 4 ... Optical axis 5 ... Illumination light source 6 ... Illumination system lens 7 ... Imaging lens 8 ... Lens barrel binocular part 9 ... Image position 10 ... Light shielding member 11 ... Illumination light source 12 ... Illumination System lens 13 ... Light shielding member 14 ... Imaging lens 15 ... TV camera for ultraviolet light observation 16 ... Imaging position 21 ... Stage 22 ... Drive circuit 23 ... Control circuit 23a ... Storage means 24 ... Operation part 25 ... Half mirror 26 ... Dichroic mirror 29 ... Sample 31-35 ... Object selection switch 36 ... Encoder dial 41 ... Ultraviolet light half mirror 42 ... Ultraviolet light mirror 51 ... Camera controller 51a ... Camera controller 52 ... Capture board 53 ... Computer 54 ... Monitor 55 ... Cable 56 ... Software 101: dimming dial 102: dimming unit 61: image input unit 6 ... Resource management unit 63... Image display unit 64... Microscope control unit 65. 66e ... "REMOTE OFF + BF" button 66f ... indicator 66g ... indicator 111 ... TV camera for visible light observation 112 ... beam splitter

Claims (2)

[Claims] 1. A microscope body having a magnifying optical system for macroscopic observation and a magnifying optical system for observing an electronic image by a microscope observation barrel, and an operation image for operating a microscope. A microscope apparatus, comprising: a control unit that controls switching between a magnifying optical system for visual observation and a magnifying optical system for observing the electronic image with respect to the microscope main body using a microscope observation lens barrel. 2. The microscope main body is capable of performing a switching operation on the operation image by a login signal from the control unit, and the control unit is configured to log in and perform the electronic operation by one operation on the operation image. 2. The microscope apparatus according to claim 1, wherein a switching operation to a magnifying optical system for observing an image, and a switching operation to a magnifying optical system for macroscopic observation by a log-off and a microscope observing barrel are enabled.