JP2001272392A - Method for measuring physiological function and testing food - Google Patents

Abstract

PROBLEM TO BE SOLVED: To enable precise measurements on physiological functions for clearly evaluating and managing the features and problems to life-style related diseases by individuals. SOLUTION: Testing food material A is material A is dished out by a moderate weight according to the amounts of food ingested in the morning, noon and evening for the past several weeks of each subject as a tested subject, and testing food C for three days, that is, nine times in total is prepared. Raw grains of rice (30 grains/time) colored in nine colors and easily discriminated are mixed with the testing food C. Only this testing food C by each subject and designated drinks are taken in by the subjects, from whom urine D' and blood F' have been sampled immediately before the test start under the normal living conditions (three consecutive days), and immediately before and after the end of the third day's test, the subjects, urine D1, D2, excrement E1, E2, and blood F1, F2 are sampled. Various measurements are made on the samples of the of three kinds and eight specimens.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for measuring physiological functions and a food for testing, and more particularly to a physiological function for each individual (digestion, absorption, metabolism, etc., from ingestion to excretion after ingestion of food or drink). The present invention relates to a physiological function measuring method and a test food for measuring and evaluating the characteristics and problems of each person with respect to lifestyle-related diseases by measuring physiological activities and functions centered on the subject.

[0002]

2. Description of the Related Art In recent years, people's eating habits have become rich and varied. However, in this complicated society, people are more likely to fall into a so-called "lifestyle-related disease" in which eating habits such as unbalanced eating, overeating, anorexia, and lack of exercise have become habitual. For example, in the case of Japanese eating habits, overdose has been said to be overdose for about 40 years since the 1960s. In particular, obesity, diabetes, hypertension, hyperlipidemia, arteriosclerosis,
It is said that the number of patients with lifestyle-related diseases such as some cancers (breast cancer, rectal cancer, etc.) exceeds 13 million including potential ones. Furthermore, the rate of increase in national medical expenses in Japan exceeds that of GNP, and new development of daily health assessment and management is required.

[0003] As referred to as "medical and dietary sources", it is very important to precisely measure dietary habits, digestion, absorption, metabolic functions, and the like for each individual in maintaining and managing a comfortable health condition. . In particular, maintaining and maintaining the health of elderly people due to uneven eating and overeating
In management, there is a long-awaited need for a method to determine the great individual differences caused by the influence of lifestyles over many years, rather than using general theory and average values, to determine them more rigorously and to use them in individual treatment and guidance. I have.

[0004]

In recent years, attention has been paid to Taylor made treatment in which the gene of each patient has been precisely analyzed. It is considered that it is difficult to discriminate between the genes because the direct relationship is poor from the gene. Specifically, regarding Taylor made treatment, gene analysis using "DNA chips" has been promoted in recent years, but genes only determine innate initial conditions of individuals,
Although there is an indirect role in the actual condition of acquired lifestyle-related diseases, it is not possible to grasp and identify the physiological activities and physiological functions of individuals. Furthermore, the physiological activities and physiological functions of individuals are not fixed, and aging, season, living environment,
Due to the personality that easily changes due to various stresses, the identification of individual physiological activities and physiological functions due to lifestyle is not a single measurement, but may be performed at regular time intervals or You need to do it regularly.

[0005] Research and development of functional foods and the like have enabled detailed analysis of food components (inputs to the digestive organs) down to the molecular level. It is becoming possible to generate molecules that fulfill the role and function of. In addition, although a great deal of knowledge has already been accumulated regarding the components of blood and urine, the precise compositional relationship between ingested foods and stool with physical conditions and diseases is extremely important physiologically. This is an area in which a huge amount of unknown research issues remain if the target is to cover functional characteristics of individuals.

Further, development of biosensors for various biochemical components such as components of blood, urine, stool, etc. (output from digestive organs), and simple quantitative measurement technology by near infrared spectroscopy, etc. And the rapid development of simple analysis and processing of enormous data due to the increase in capacity of storage media such as computer hard disks,
Currently, we are facing the stage where significant personal information can be extracted from a vast amount of information resources on various physiological activities of the human body.

[0007] In view of the above, the present invention measures a physiological function for each individual and clearly evaluates and manages the characteristics and problems of each person with respect to lifestyle-related diseases and the like, and a method and test for measuring physiological functions. The purpose is to provide food for use.

[0008]

According to a first aspect of the present invention, a test food material to which a component serving as an index is added corresponding to a specific disease is generated, and the test food material is prepared in advance. For a subject from which a sample for measuring a specific physiological function was collected, set aside a quantity based on daily food intake, generate a test food, and generate the generated test food for the subject. After taking a predetermined period and number of times, a sample for measuring a specific physiological function is collected from the test subject, and based on the collected sample, a specific digestive system or circulatory system disease of the test subject. The present invention provides a physiological function measurement method for determining a state of a physiological function.

According to a second solution of the present invention, there is provided a test food to be taken by a subject for measuring a specific disease of the subject, comprising: a basal diet mainly composed of vitamins and minerals; Fiber, albumin, xylose, containing a flavor component and water, for a subject from which a sample for measuring a specific physiological function was previously collected,
To provide a test food characterized by being divided into quantities based on daily dietary intake.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION Overview The present invention is directed to the basic concept of a series of “measurement (inspection), evaluation, and judgment systems”. First, an outline of an inspection system according to the present invention will be described. The test system selects test subjects' test foods, grasps the subjects' eating and living conditions, and collects and analyzes the subjects' blood, urine, feces, and the like. Further, based on the analysis result, evaluation, diagnosis, and treatment guidelines for individual physiological functions are determined (this test / determination can also be performed on a daily basis by a subject).

For a certain period of time (for example, about 3 to 10 days), the subject is asked to give “chemically adjusted food (depending on the disease to be tested)” instead of general food.
Take a “comprehensive test” under normal living conditions while ingesting (however, if the subject is severely ill, this test can be taken while in the hospital). The subject can freely take water as long as the amount is measured. In addition, the acceptability (ease of eating) of processed and adjusted foods (foods adjusted in terms of chemical components) to be ingested over several days has been greatly improved by recent advances in manufacturing technology.

Next, samples (blood, urine, stool, etc.) from the subject collected at several points immediately before, during, and after the test and the current medical condition are numerically described as " By performing "precision analysis", the physiological activities and physiological functions related to digestion, absorption, metabolism, etc. of the subject are determined and evaluated. For example,
In addition to biochemical components such as saliva, daily activities (sleep and wake-up time, number and volume of eating and drinking and urinary defecation, exercise amount, etc.) and indicators (physical temperature, blood pressure, heart rate) , Respiratory volume, electrocardiogram, etc.). The main test items of urine are pH, specific gravity, protein, glucose, ketone body, occult blood reaction, bilirubin, urobilinogen, nitrite, leukocyte reaction, ascorbic acid, bacteria (intestinal bacteria, Pseudomonas aeruginosa,
Staphylococci and the like). The main test items for feces are occult blood reaction, fat, bilirubin / urobillin, enzymes (trypsin / chymotrypsin, etc.), bacteria (diarrheal Escherichia coli, anaerobic bacteria, pathogenic bacteria (vibrio, salmonella, yersinia, etc.)) ).

[0013] Furthermore, the present invention organizes the advantages and disadvantages of each individual's lifestyle habits, as well as points of improvement and problems, based on the results obtained by the above-described tests and various surveys on subjects mainly on eating habits. It can be evaluated and applied to treatment and lifestyle guidance. In addition, as for these various investigations, quantitative measurement of chemical components contained in urine and feces is mainly performed, and statistical analysis using the above-described biosensor technology, DNA center technology, or nondestructive near-infrared spectroscopy is performed. More accurate and quick measurement can be performed by a rapid quantitative measurement method or the like.
The lifestyle habits for each individual include the causes of lifestyle-related diseases, such as the subject's past illness, the medical history of parents and siblings, the place of residence and life, the likes and dislikes of food, the degree of smoking habits, and the content of daily eating. There are various factors that are considered to be.

Furthermore, since the physiological function of an individual is not fixed, the physiological activities of the individual may be reduced in a short time due to various stresses from the living environment, sleep, eating, drinking and smoking. Because of the possibility of drastic fluctuations, not only comprehensive and precise measurement and evaluation of individual physiological functions to be treated, but also a simple measurement and evaluation system that allows ordinary citizens to manage themselves at home on a daily basis. Is also required. For example, the “comprehensive test” often depends on the content and symptoms of the disease, but often needs to be performed “once every 6 to 15 months”. On the other hand, in a simple measurement / evaluation system in which ordinary citizens can self-manage at home on a daily basis as in the present invention, the “individual daily management” of the disease / symptom specified by the above-described test results is performed. The main goal is to be able to conduct tests easily and with a frequency of "once / about 2 to 4 weeks"
Furthermore, it can be reliably executed at home such as a patient. Specifically, it is not a comprehensive test that takes several days to perform, but it is intended to manage the daily life of subjects who show very mild signs of illness. It is possible to provide a simple test for “specific items” so that a conclusion can be made.

Further, in the present invention, a self-health condition is accurately grasped by ingesting a food prepared and formulated as a chemical component and performing a self-test on urine and feces. Specifically, a component serving as an index is added to the food to be ingested, and signs (appearance most easily determined by a pigment or the like) appearing in urine, feces, etc. are identified and evaluated based on their fate and metabolism in the digestive tract.

Further, the food prepared and formulated as a chemical component in the present invention is divided into several types of “universal test foods (main components, subcomponents, indicator components, etc.) in accordance with the standard disease content. In addition to the above-mentioned “combination by subject”), “tailor-made test foods” are manufactured and supplied according to the disease condition targeted by each individual. Specifically, by classifying the physiological function of the subject into several types according to the disease to be treated, the change of each index component in the digestive tract can be inspected on a daily basis (just like the “model of ready-made clothes”
Like, you can understand your size and its fluctuations).

2. Composition of Foods Taken by Subject (1) Foods taken by the subject are foods containing specific components, vitamins, and minerals that have been chemically and / or biochemically and / or microbiologically treated. Before, during, and after ingestion, the physical physiological activities of the subject such as blood, urine, feces, and saliva are collected, examined, and evaluated. (2) The food consumed by the subject is a food mainly composed of carbohydrates that have been chemically and / or biochemically and / or microbiologically treated. (3) Foods to be ingested by the subject are foods mainly composed of chemically and / or biochemically and / or microbiologically processed proteins. (4) The food that the subject ingests is a food containing mainly lipids that have been chemically and / or biochemically and / or microbiologically treated.

3. Specific inspection method 3-1. First Test Method The first test method is, for example, a test method of a physiological function by a predetermined group for three days (9 meals). First, on a weight basis, 0.3% of a basal diet mainly composed of vitamins and minerals, and a molecular weight of 100,000-1
8.7% of mannan (dietary fiber) divided into 50,000, 2.2% of albumin divided into molecular weight of 30,000 to 55,000, 1.8% of xylose, 0.05% of flavoring component, and the rest A test food material (A) filled to 100.0% with water is prepared.

The test food material (A) is divided into appropriate weights based on the amount of food consumed by each subject to be examined during the past few weeks in the morning, noon, and evening, and three days for a total of 9 meals.
Make test food (C) twice. Further, the test food (C) is mixed with raw rice grains (30 grains / time) which are colored in nine colors and are easily separated. Only the test food (C) and the predetermined beverage for each subject were subjected to urine (D ') and blood (F') immediately before the start of the test.
Ingestion was performed under normal life (for three consecutive days), and immediately before and immediately after the end of the test on the third day, urine (D
1, D2), feces (E1, E2), blood (F1, F2)
Was collected.

With respect to the collected three kinds and eight samples, not only their weights but also urine (D ′ → D1, D
Regarding 2), the concentrations of pH, protein, glucose, ketone body, bilirubin, urobilin body, urea nitrogen, and electrolyte were measured. For feces (E1, E2),
Immediately after defecation, the coloration and number of the colored rice grains discharged, the concentration of fat, occult blood reaction, bilirubin, urobilin body, and the pH of the stool solution diluted about 4 times with water, and the average of 23 ° C
The pH was measured every 7 hours until the end of 1.5 days.

For blood (F ′ → F1, F2), lipid test (T-cho: total cholesterol level, TG: triglyceride level, HDL-cho: HDL cholesterol level),
Liver function test (ZTT: zinc sulfate opacity test (zinc sulfate
turbidity test), GOT (glutamic-oxalacetate tran
saminase), GPT (glutamic-pyruvate transaminase),
The concentrations of γ-GTP (γ-glutamyl transpeptidase), renal function tests (uric acid, creatinine), and general blood tests (blood sugar, serum amylase, white blood cell count, red blood cell count, memoglobin amount, hematocrick value) were measured.

By performing a multiple regression analysis by integrating the numerical values of these measurement results, a group of subjects who have not already been treated for diabetes and hypertension, as well as subjects who have not yet exhibited specific symptoms, can be classified into “ There are three groups: a group that needs immediate attention in terms of lifestyle (red light), a group that occasionally checks their health (yellow light), and a group that does not need to worry for the time being (green light). Can be separated.

3-2. Second inspection method In the second inspection method, for example, the inspection is completed after about three to four meals, thereby achieving the “self-inspection” of the amateur.
First, by weight conversion, it was divided into 0.3% of basal diet mainly composed of vitamins and minerals, 8.7% of mannan divided into 100,000 to 150,000 molecular weight, and 30,000 to 55,000 molecular weight. Albumin 2.2%, xylose 1.8
%, Flavoring ingredient 0.05%, the rest is filled with water 10
A test food (P) having a concentration of 0.0% is prepared.

The test food (P) was tested on subjects classified as "yellow light" and "green light" as a result of the first inspection method described above, with four meals of late morning, noon and evening. Food (P) and beverages only. On the morning following the end of the intake, urine (Q) and feces (R) are collected from the subject. For urine (Q), protein and sugar concentrations were measured using urine test paper. For feces (R), the occult blood reaction is measured by a fecal occult blood test reagent, and the pH of a stool solution diluted about 4 times with water is measured immediately after defecation and twice a day after using a pH test paper.

From the change in pH during this period, the subject directly makes a self-judgment of the state of the physiological function of the digestive tract system of the individual, and judges whether or not each of them needs to make immediate contact with doctors. it can.

3-3. Third Inspection Method First, in terms of weight, 0.3% of basal diet mainly composed of vitamins and minerals, 8.7% of mannan divided into 100,000 to 150,000 molecular weight, and 30,000 to 5.5 molecular weight Albumin 2.2%, xylose 1.8 divided into 10,000
%, Linolenic acid with a purity of 95% or more 1.2% (I) / 5.
2% (II) /9.6% (III), flavor component 0.05
%, The remainder being filled with water to make 100.0%, to produce three types of test food materials (AI, A-II, A-III).

This test food material (AI, A-II, A
-III) was adjusted to an appropriate weight based on the amount of food consumed by the test subjects (three patients with mild hyperlipidemia and three healthy subjects, a total of six) in the morning, lunch, and night in the past week. Together with
Make test food for 3 days, 9 times in total (C-I, C-II,
C-III). Furthermore, test foods (C-I, C-II, C-
III) was administered to 6 subjects each for 3 consecutive days.
On the day, (C-I) is given, with a week
On the day, (C-II), and further about one week apart, on the third day, (C-III), they were fed three times, just before each meal, the first day, the second day On the third day, four times, blood,
Urine and feces were collected and their components were examined in detail. According to the results of this study, the most obvious changes appeared in lipid components in blood. Test food with the lowest lipid (linolenic acid) content (C-I) and test food with the highest content (C-III)
3 days later, about three times the change in the lipid component in the blood was observed. The minute deviations between the components in urine and feces were extremely complicated, and none of them protruded independently.However, the dynamics of blood gastrin, a gastrointestinal hormone, and glucagon, a pancreatic hormone, were not observed. , A clear deviation appeared.

3-4. Fourth inspection method First, in terms of weight, 0.3% of basal diet mainly composed of vitamins and minerals, 8.7% of mannan divided into 100,000 to 150,000 molecular weight, and 30,000 to 5.5 molecular weight Albumin 2.2%, xylose 1.8 divided into 10,000
%, Linolenic acid having a purity of 95% or more, 1.2% of flavoring components, and the rest being filled with water to make a test food material (A-I) of 100.0%.

The test food material (A-I) was tested for subjects (three mild diabetic patients, three healthy subjects,
Based on the amount of food consumed in the morning, noon, and evening for the past week (6 persons in total), the food is divided into appropriate weights, and the test food (C-I) is prepared 9 times in total for 3 days. Further, when conducting an eating test of the test food (C-I), raw soybeans (3 grains / time), which are colored in 9 colors (LT) and easy to separate, are mixed with L-colored raw soybeans. The subjects swallowed the grains in the order of the grains (10 minutes before each meal), three M-colored raw soybeans (midpoint of feeding), and three N-colored raw soybeans (15 minutes after eating). In order to use 9 colors of raw soybeans in one day, for example, L, M, N colors in the morning, O, P, Q colors in the daytime,
In the evening, eat raw R, S and T colored soybeans. Further, on the second and subsequent days, the subjects are again ingested 9 colors (LT) of raw soybeans as in the first day, and the same test is repeated on the third day. As a result, the order in which the soybeans contained in the excreted feces came out of the body was examined, and based on the order, the physical mixing state in the intestinal tract of the subject was examined.

Also, when returning to a normal diet immediately after the end of the feeding test, a raw soybean swallow test for L to T colors was added in the same manner for 2 days, and mixing in the digestive tract due to the difference in the content of the feeding. I checked the condition. As a result, between the diabetic patient and the healthy subject, a remarkable difference was observed in the mixed state of the contents in the intestinal tract. In other words, diabetic patients are referred to as the so-called "piston flow type" (flowing fluid having a flow velocity distribution perpendicular to the pipe axis, similar to the movement of a piston cylinder when fluid flows through the pipe). A phenomenon called so-called “back-mixing” (when fluid flows through a tube, a part of the flowing fluid appears as a whole) A phenomenon referred to as "returning to the upstream part in reverse to the flow of") was observed. In addition, by numerically analyzing the eating and discharging processes of colored raw soybeans, the flow state involving mixing in the intestinal tract can be quantitatively expressed.

The types and amounts of the components of the test food material used in the above-described inspection method can be appropriately changed as long as they can be measured. In addition, the test food material, for example, water-soluble, water-insoluble or both dietary fiber, or depending on the disease of interest, other appropriate components, or remove unnecessary components, etc. Can be changed. Further, if the rice grains and raw soybeans are not digested and decomposed and can be marked with coloring or the like, other food materials can be used as appropriate. On the other hand, the number of colors is not limited to nine colors as long as accurate measurement is possible. In addition, the feeding timing can be set at an appropriate timing and number of times as long as accurate measurement is possible.

[0032]

According to the present invention, it is possible to accurately measure digestive and physiological functions for clearly evaluating and managing the characteristics and problems of each person with respect to lifestyle-related diseases.

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Claims (9)

[Claims] 1. A subject who produces a test food material to which a component serving as an index is added in accordance with a specific disease, and from which a sample for previously measuring a specific physiological function is collected from the test food material. In contrast, the amount is divided into the amount based on the daily dietary intake, a test food is generated, and the generated test food is ingested to the subject for a predetermined period and a number of times. A method for measuring physiological functions, wherein a sample for measuring a specific physiological function is collected, and a state of the subject for a specific digestive system or circulatory system disease is determined based on the collected sample. 2. The method for measuring physiological functions according to claim 1, wherein the test food taken by the subject is a food mainly containing one of carbohydrates, proteins, and lipids. 3. The method for measuring physiological functions according to claim 1, wherein granular foods such as raw rice grains or soybeans are further ingested as the test foods. 4. The sample for measuring the specific physiological function is urine or stool of a subject, and measures the concentration of a specific component such as protein and sugar in the urine, and specifies the occult blood reaction of the stool. Measuring the components, the subject, based on the measurement results of the urine and feces,
The physiological function measuring method according to any one of claims 1 to 3, wherein the state of the physiological function of the digestive tract system is determined by itself. 5. When the test food material is produced, one or more test food materials are produced according to the amount of a material containing lipid such as linolenic acid, and The method for measuring physiological functions according to any one of claims 1 to 3, wherein a plurality of kinds of the test food materials are ingested for a continuous number of days separated by a period. 6. A method in which the subject is caused to ingest a plurality of granular foods such as raw soybeans or rice grains colored in different colors at predetermined intervals, and the condition of the subject for a specific disease is determined. 4. The physiological function measuring method according to claim 1, wherein the mixed state in the intestinal tract of the subject is inspected based on the order or the number of raw soybeans or rice grains discharged from the subject. 7. A test food ingested by a subject for measuring a specific disease of the subject, comprising: a basal diet mainly composed of vitamins and minerals; dietary fiber; albumin; xylose; A test food comprising a flavor component and water, the test food being provided in a quantity based on daily dietary intake for a subject from which a sample for measuring a specific physiological function has been collected in advance. 8. The test food according to claim 7, further comprising a lipid such as linolenic acid. 9. The test food according to claim 7, further comprising a plurality of granular foods such as raw soybeans or rice grains colored in different colors.