JP2001247422A - Bacterium for killing larva of fly, agent for killing larva of fly, and method for killing larva of fly - Google Patents

Abstract

PROBLEM TO BE SOLVED: To efficiently kill a fly, especially the one in a larva stage by a method safe to humans and animals without using a chemical material (an insecticide). SOLUTION: This method for killing the larva of the fly is characterized in that the larva of the fly is killed by Bacillus subtilis at a temperature region from a low temperature to a normal temperature and killed by the heat in a high temperature region caused by Bacillus stearothermophilus by scattering bacteria including the Bacillus subtilis and the Bacillus stearothermophilus on accumulated excrement of beasts.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a poultry farm, a pig farm,
Flies occur on ranches and other places where livestock dung is deposited,
For sterilization of fly larvae to eradicate flies, especially adult worms, which are the first of the unsanitary insects by transmitting pathogens, etc., and a killing agent containing the same and a method for killing fly larvae It is.

[0002]

2. Description of the Related Art Chemical substances (pesticides) to eradicate flies
Have been used for many years. For this reason, the insects have become resistant to fly larvae and adults, and the insecticidal effect of chemicals has been weakened. In addition, recently, the use of chemical pesticides has been often pointed out to have an adverse effect on the human body. This is particularly problematic for those with chemical sensitivity. Therefore, JP-A-6-72819 proposes a fly killing agent containing actinomycetes or an actinomycete culture as an active ingredient.

[0003]

In the case of using an actinomycete or an actinomycete culture, the actinomycete is incorporated into bacteria in classification, but the morphology of the fungus is remarkably different from that of general bacteria. Similar to kind. Reproduction rate is slower than bacteria. Therefore, when used in food waste or food factory waste to eradicate flies, disgust cannot be avoided early because the effect of killing flies is small or it does not work up to the pupal stage of fly larvae. Also, depending on the place of use, actinomycosis may occur in humans and livestock depending on the bacterial species.

Therefore, a bacterium which is safe for humans and animals without using a chemical substance (pesticide) and which can efficiently eradicate flies especially at the stage of larva was studied.

[0005]

As a result of studying the above-mentioned problems, a bacterium capable of killing fly larvae mainly comprising Bacillus subtilis was found. This Bacillus subtilis is Bacillus subt.
ilis) and Bacillus stearothermophilus (Bacillus ste
arothermophilus) or a mixed bacterium thereof, and the B. subtilis AT-
3 strains (accession number FERM P-16073, hereinafter abbreviated as AT-3 strain). Bacillus subtilis AT-3 has an optimum growth temperature of 37 ° C, and Bacillus stearothermophilus AT-3 has an optimum temperature of 55 ° C. Both taxonomically Bacillus
(Bacillus). It is believed that the mixing of such two bacterial species produces a synergistic effect. As a result, insects to be excluded are at least flies, cockroaches, house dust mites and the like even if they are confirmed. The bacterium containing this Bacillus subtilis was cultured in chicken dung and used as a killer for fly larvae composed of fermented chicken dung.

[0006] The method of killing fly larvae is characterized by spraying the bacteria containing Bacillus subtilis on the deposited feces. In addition, the bacteria containing Bacillus subtilis and Bacillus stearothermophilus are sprayed on the deposited feces to kill fly larvae at low to normal temperatures with Bacillus subtilis, and at high temperatures with Bacillus stearothermophilus. A method for killing fly larvae, characterized by killing fly larvae by heat, was developed.

[0007]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Culture of Bacillus subtilis alone Bacillus subtilis was cultured in a brain heart infusion broth at 37 ° C. for 72 hours to obtain a stock solution of Bacillus subtilis. This was further cultured at 37 ° C. for 72 hours with the composition shown in Table 1 below in order to multiply this mass.

[0008]

[Table 1]

Cultivation of Bacillus stearosamophilus alone Bacillus stearosamophilus was cultured in a brain heart infusion broth at 55 ° C. for 48 hours to obtain a stock solution of Bacillus stearosamophilus. This was further cultured at 55 ° C. for 72 hours with the composition shown in Table 2 below in order to propagate it in large quantities.

[0010]

[Table 2]

Preparation of a mixed strain of Bacillus subtilis and Bacillus stearosamophilus A mixture of 7 tons of raw chicken manure and 30 kg of brewer's yeast and 3 kg of glucose was mixed with 45 L of the above stock solution of Bacillus subtilis and 30 L of Bacillus stearosamophilus stock solution and stirred. Into a 48 cubic meter fermenter equipped with
The temperature was maintained at 37 to 40 ° C., and then fermented at 55 ° C. for 72 hours.

Killing example 3 of fly larvae A fly larva was placed in a petri dish, and the above-mentioned undiluted solution of Bacillus subtilis alone and undiluted solution of Bacillus stearosamofilus were sprayed in separate Petri dishes to such an extent that the body of the fly larva became wet. As a result, the fly larva was completely annihilated at room temperature in the case of the undiluted solution of Bacillus subtilis. In the stock solution of Bacillus stearosamophilus, fly larvae were completely annihilated at a high temperature maintained at 50 ° C.

Example 4 of killing of fly larvae About one square meter of cow dung on which a mixed strain of Bacillus subtilis and Bacillus stearosamofilus was deposited.
~ 1.5Kg was sprayed. As a result, the larva died. By spraying every 30 days, the occurrence of flies could be almost suppressed.

Culture of AT-3 strain Chicken manure was deposited at room temperature and allowed to stand for about 2 weeks, but a white cheese-like mass of hyphae was grown on the top. This was cultured in a culture vessel at 37 ° C. for 2 days using bouillon. The composition of the bouillon is shown in Table 3 below.

[0015]

[Table 3]

After repeating the cultivation of broth from the formed colonies a plurality of times, the following test was carried out.

Killing example 1 of fly larvae In the summer when flies are most likely to occur, leftovers, fish, vegetables and other household waste were deposited. Since flies began to collect when rot started a little, the AT-3 strain stock solution was sprayed uniformly at 1 L per 50 kg of trash. As a result, the fly died at the larval stage, and no adult emerged.

Killing Example 2 of Fly Larvae A Bacillus subtilis AT-3 strain (Accession No. FERM P-16073) deposited by the present inventor at the Patent Microorganisms Depositary Center was used as a culture solution similar to the above example, and this was used as a compost. Spread 2L uniformly per 150Kg of compost in the field. As a result, the fly dies at the larval stage,
There was no adult outbreak.

[0019]

Industrial Applicability According to the present invention, it is possible to safely eliminate human flies and eliminate flies efficiently without using a chemical insecticide.

Livestock and livestock,
It is a major contribution to the promotion of industries such as poultry raising, swine raising, and fertilizer production and improvement of hygiene.

Claims (5)

[Claims] An insecticide for fly larvae comprising a bacterium containing Bacillus subtilis, a bacterium belonging to the genus Bacillus. 2. A bacterium containing Bacillus subtilis is an AT-3 strain (Accession No. FERM P-160) deposited at the Patent Microorganisms Depositary.
73) is an insecticide for fly larvae. 3. A bacterium containing Bacillus subtilis.
Or an insecticide for fly larvae consisting of fermented chicken dung produced by culturing chicken dung with the fungus of 2 above. 4. A method for killing fly larvae, which comprises spraying the bacteria containing Bacillus subtilis according to claim 1 on sediment. 5. Spraying the bacteria containing the Bacillus subtilis and Bacillus stearothermophilus according to any one of claims 1 to 3 against the deposited feces, and killing the fly larvae at a low to normal temperature range with the Bacillus subtilis. A method for killing fly larvae, wherein the fly larvae are killed by heat in a high temperature range by using Bacillus stearothermophilus.