JP2001213757A - Melanin production inhibitor and beautifying cosmetic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a melanin production inhibitor originated form natural materials high in safety, and a cosmetic given the beautifying function by blending this inhibitor. SOLUTION: The melanin production inhibiting functions of an extract obtained from the root part of Ricinus communis by extracting with water, a fatty lower alocohol, 1,3-butylene-glycohol or mixture of them as an extracting solvent, is utilized.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a novel melanin production inhibitor and a cosmetic having a whitening effect.

[0002]

2. Description of the Related Art Tyrosine, which is a kind of amino acid, produces a dark brown or reddish brown pigment or melanin by the action of tyrosinase, which may cause spots or freckles on the skin. Therefore, attempts have been made to prevent melanin production by using a substance having an action of suppressing melanin production by some mechanism such as inhibiting the action of tyrosinase, and as a result, various melanin production inhibitors have been found. It has been proposed and used as an additive in cosmetics and foods. Representatives are ascorbic acid, sulfur, hydroquinone, kojic acid, and natural plant extracts.

[0003] However, ascorbic acid has a problem in terms of stability when water is present, and sulfur has a drawback due to its peculiar odor. Hydroquinone is remarkably effective but highly toxic. Kojic acid and plant extracts are high in safety but weak in tyrosinase inhibitory action and melanin production inhibitory action, and many plant extracts have problems in terms of smell, color, and the like.

[0004] The castor root (referred to as castor root in Chinese medicine) used as a raw material for extracting a melanin production inhibitor in the present invention described below is trans-2-decene-4,
6,8-triic acid methyl ester, 1-tridecene-
It is known that it contains a physiologically active substance such as 3,5,7,9,11-pentiin and β-sitosterol, and is used for treatment of pain due to tetanus, tencan, rheumatism and the like. It has nothing to do with suppression of melanin production.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to find a melanin production inhibitor derived from a natural product, and to provide a melanin production inhibitor having high safety and which can be used in cosmetics and foods with a sense of security.

[0006] Another object of the present invention is to provide a cosmetic product having a whitening effect provided by a highly safe natural substance-derived melanin production inhibitor.

[0007]

DISCLOSURE OF THE INVENTION The present invention relates to a plant of the family Euphorbiaceae, Ricinus communi.
s L. A) a melanin production inhibitor and a whitening agent, characterized by containing as an active ingredient a melanin production inhibitor which is contained in the root of water, an aliphatic alcohol, 1,3-butylene glycol or a mixture thereof. To provide cosmetics.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Castor is cultivated in India or the like in order to collect fatty oil from its seeds. Therefore, the normally discarded portion after seed collection is used as a raw material for extracting a melanin production inhibitor. be able to.

The details of the melanin production inhibitor specifically contained in the castor root are unknown, but the cut or ground castor root is composed of water, lower aliphatic alcohols (eg, methanol, ethanol), 1,3-butylene glycol. , Or when they are extracted with a mixture thereof, they are easily eluted. A preferred extraction solvent is the above-mentioned hydrophilic organic solvent or a mixture thereof with water. Although the extraction conditions and the apparatus used for the extraction are not particularly limited,
Preferably, the castor root (whether raw or dried) is immersed in 5 to 15 times by weight of the above-mentioned extraction solvent, and soluble while stirring gently at room temperature to about 100 ° C. Elute the components. The extract obtained by filtration or centrifugation is appropriately concentrated and dried. The resulting extract may be used as it is as a melanin production inhibitor, but if necessary, activated carbon treatment, adsorption resin treatment, as long as the melanin production inhibitory action is not reduced.
The preparation may be purified by ion exchange resin treatment or the like, and further mixed with an optional auxiliary agent, solvent, or the like, and then formulated.

[0010] When a castor root extract having a melanin production inhibitory action is blended into a cosmetic to form a cosmetic having a whitening effect, the preferred blending ratio of the extract is determined by the strength of the castor extract used and the activity of the cosmetic. Although it varies depending on the type, the content is approximately 0.0001 to 1.0% by weight.

[0011] By blending the castor root extract, the selection of other cosmetic components is not restricted,
Oily components such as avocado oil, rice bran oil, rice germ oil, lanolin, squalane, beeswax; glycerin;
Humectants such as 1,3-butylene glycol, collagen, hyaluronic acid and its salts, chondroitic acid and its salts, chitin and chitosan; complex lipids such as glycerophospholipid, sphingolipid, glyceroglycolipid, and glycosphingolipid; superoxide Active oxygen-scavenging substances such as dismutase, catalase, β-carotene, ginkgo biloba extract, vitamin C and its derivatives, vitamin E and its derivatives, gougon extract and kujin extract; guaiazulene, kama azulene and its derivatives, glycyrrhizic acid and Its salts, glycyrrhetinic acid and its salts or derivatives, anti-inflammatory substances such as zinc oxide; arnica extract, cinnamon extract, gibbon extract, oak extract, spinach extract, chamomile extract, licorice root extract, Sa Shish extract, siconium extract, peonies extract, peanut extract, peanut extract, birch extract, western horse chestnut seed extract, calendula extract, peanut extract, sapling extract, linden extract, rosemary extract Stuff, Sawtooth grass extract, Artemisia extract, Iwan Chinese cabbage extract, Yokuinin extract,
Aloe extract, loquat extract, peach extract, senkyu extract, sage extract, touki extract, loofah extract, eucalypt extract, panax ginseng extract, capsicum extract, bean tea extract, tea extract, hypericum Extract, horse chestnut extract, prune extract, collagen,
Hydrolyzed conchiolin, elastin, vitronectin,
Fibronectin, placenta extract, bifidobacterium culture, lactic acid bacteria culture, litchi mycelium extract, kuryo extract, brown algae extract, red algae extract, green algae extract, etc.
Plant extracts, animal extracts, microbial components or algal extracts that are expected to show some favorable effects on the skin; other various vitamins, thickeners, preservatives,
An ultraviolet absorber, a fragrance, an antioxidant, and the like can be arbitrarily selected as constituent components.

Representative forms of the whitening cosmetic composition according to the present invention include, but are not limited to, ointments, creams, emulsions, lotions, packs and the like.

[0013]

The present invention will be described below with reference to examples. Production Example 1 500 g of an 80% aqueous ethanol solution was added to 50 g of the dried crushed castor root, and the mixture was subjected to a reflux extraction treatment with a reflux extractor for 3 hours. The obtained extract was filtered, concentrated under reduced pressure, and freeze-dried to obtain 2.1 g of a powdery extract.

Production Example 2 500 g of water was added to 50 g of the dried crushed castor root, and the mixture was subjected to reflux extraction for 3 hours with a reflux extractor. The obtained extract was filtered, concentrated under reduced pressure, and freeze-dried to obtain 5.9 g of a powdery extract.

Production Example 3 50 g of dried sesame seeds were ground with 50 g of ethanol.
0 ml was added, and the mixture was subjected to a reflux extraction treatment with a reflux extractor for 3 hours. The obtained extract was filtered, concentrated under reduced pressure, and freeze-dried to obtain 1.4 g of a powdery extract.

Production Example 4 500 g of 1,3-butylene glycol was added to 50 g of the dried crushed castor root, and extracted at 95 ° C. for 3 hours. The obtained extract is filtered and then cooled to 5 ° C.
The mixture was allowed to stand for a day, and the formed deposits and precipitates were filtered using diatomaceous earth to obtain a clear extract (solid content concentration: 0.13%) 430
ml were obtained.

Production Example 5 50 g of a 50% 1,3-
500 ml of a butylene glycol aqueous solution was added, and extraction was performed at 95 ° C. for 3 hours. After filtering the obtained extract, it was allowed to stand at 5 ° C. for 5 days, and the formed deposits and precipitates were filtered using diatomaceous earth to obtain a clear extract (solid content of 0. 0%).
440 ml of (50%) were obtained.

Test Example 1 The extract obtained in each of the above examples was tested for its melanin production inhibitory effect using B-16 melanoma cells. The test method is as follows. A small amount of the extract obtained in Production Examples 4 and 5 was concentrated under reduced pressure and freeze-dried to obtain a dry powder, which was subjected to a test.

Melanin production inhibition test: Medium (10% FB)
S-DURBECCO medium; the same applies hereinafter) 1 × 10 B-16 melanoma cells in a 25 ml flask containing 5 ml
^Six seeds are inoculated and pre-cultured for 5 days in a 37 ° C. incubator adjusted to a CO ₂ concentration of 5%. The cells are then trypsinized and centrifuged to collect the cells. Obtained cells 4x
10 ⁶ cells are inoculated in a Petri dish with a diameter of 60 mm containing 5 ml of medium, cultured for 24 hours, and then cultured for 3 days in a 1 mM theophylline-added medium in which a sample is dissolved to a predetermined concentration. Separately, cultivation is performed using a sample-free medium, and the same operation is performed thereafter. After the culture, the cells are collected by trypsinization and centrifugation, and 2 ml of a medium is added to obtain a cell suspension.
The number of cells in the obtained cell suspension is measured using a hemocytometer, and the cell viability when a sample is added is calculated based on the number of cells when no sample is added. In addition, 5 × 10 ⁶ cells were collected from the cell suspension in which the number of cells was measured, and 10% DMSO was collected.
Ultrasonic treatment and centrifugation are performed by adding 3 ml of a -1N sodium hydroxide solution, the absorbance of the supernatant at 475 nm is measured, and the melanin production inhibition rate is calculated by the following formula.

Inhibition rate (%) = [(AB) / A] × 100, where A is the absorbance of the sample-free culture and B is the absorbance of the sample-added culture. The above test is performed by changing the sample concentration stepwise, and the sample concentration at which melanin production is suppressed by 50% is determined by interpolation. The test results were as shown in Table 1.

[0021]

[Table 1]

Example 1 The castor extract obtained in Production Example 1 was blended to produce an emulsion having the following composition by a conventional method for producing an emulsion. Castor extract (Preparation Example 1) 0.05 g Cetyl alcohol 0.5 g Beeswax 2 g POE (10) sorbitan monooleate 1 g Glycerin monostearate 1 g Sodium hyaluronate 0.1 g Propylene glycol 5 g Ethanol 3 g Ethyl paraben 0.3 g Perfume 0.03 g Purified water Remainder (total amount is 100 ml)

The following coating test was carried out on the obtained emulsion and the comparative emulsion prepared in the same manner as described above except that the castor extract was not blended. Application test: 25 to 4 with skin spots and insufficient moisture
Twenty 9-year-old female subjects were selected and bisected, and the emulsion of the example was applied to one group and the emulsion of the comparative example was applied to the other group twice a day in the morning and evening and on the face. After a lapse of three months, they were asked to comment on the results of use. Tables 2 and 3 show the results.

[0024]

[Table 2] Evaluation items in the example using the milky lotion After 3 months from the start of the application test, the skin had good moisture 0 8 8 Skin spots 10 2

[0025]

[Table 3] Comparative example Emulsion use group evaluation item Three months before the start of the application test, good moisture of skin 0 1 1 Stain on skin 10 9

Example 2 An emulsion having the following composition containing the castor extract obtained in Production Example 3 was produced according to a conventional method for producing an emulsion. Castor extract (Production Example 3) 0.05 g setosteryl alcohol 3.5 g squalane 40 g beeswax 3 g reduced lanolin 5 g ethyl paraben 0.3 g POE (20) sorbitan monostearate 2 g glycerin monostearate 2 g 1,3-butylene Glycol 5g Fragrance 0.03g Glycerin 5g Purified water Remainder (total amount is 100ml)

Example 3 A lotion containing the castor extract obtained in Production Example 2 and having the following composition was prepared in accordance with a conventional method for preparing a lotion. Castor extract (Production Example 2) 1 g glycerin 4 g 1,3-butylene glycol 4 g ethanol 7 g POE (20) sorbitan monooleate 0.5 g methyl paraben 0.05 g citric acid 0.01 g sodium citrate 0.1 g flavoring 05g purified water remainder (total amount is 100ml)

Example 4 A pack having the following composition containing the castor extract solution of Production Example 4 was produced by a conventional method. Castor extract (Production Example 4) 10 g (0.013 g as solid content) Polyvinyl alcohol 15 g Polyethylene glycol 3 g Propylene glycol 7 g Ethanol 10 g Methyl paraben 0.05 g Perfume 0.05 g Purified water Remaining (total amount is 100 ml)

Example 5 An emulsion having the following composition containing the castor oil extract obtained in Production Example 5 was produced according to a conventional method for producing an emulsion. Castor extract (Production Example 5) 10 g (0.05 g as solid content) Cetyl alcohol 0.5 g Beeswax 2 g POE (20) sorbitan monooleate 1 g Glycerin monostearate 1 g Sodium hyaluronate 0.1 g Propylene glycol 5 g Ethanol 3 g Ethylparaben 0.3 g Fragrance 0.03 g Purified water Remainder (total amount is 100 ml) The cosmetics of Examples 2 to 5 all had an effect of improving spots and were excellent in usability.

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Claims (3)

[Claims] 1. A melanin production inhibitor comprising, as an active ingredient, a castor root extract having a melanin production inhibitory action. 2. A whitening cosmetic comprising a castor root extract having an inhibitory action on melanin production as an active ingredient. 3. The castor root extract having a melanin production inhibitory action is obtained by extraction using water, an aliphatic lower alcohol, 1,3-butylene glycol or a mixture thereof as an extraction solvent. Melanin production inhibitor.