JP2001211878A - Method of culturing yogurt starter lactobacillus in high concentration, and method of producing yogurt from the obtained high concentration culture solution - Google Patents
Method of culturing yogurt starter lactobacillus in high concentration, and method of producing yogurt from the obtained high concentration culture solutionInfo
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Abstract
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、ヨーグルトスター
ター乳酸菌の高濃度培養方法、及び得られた高濃度培養
液を用いたヨーグルトの製造方法に関する。TECHNICAL FIELD The present invention relates to a method for culturing lactic acid bacteria of yogurt starter at a high concentration and a method for producing yogurt using the obtained high concentration culture solution.
【０００２】[0002]
【従来の技術】ヨーグルト製造において、乳酸菌スター
ターカルチャーの管理とその適切な使用は、ヨーグルト
の品質を決定するという点で非常に重要である。乳酸菌
スターターカルチャーには大別してバルクスターターと
濃縮スターターの２種類があるが、濃縮スターターはバ
ルクスターターと比べて取り扱いが簡便であり、管理及
び使用に高度な技術を要しないという点で望ましい形態
のスターターカルチャーである。BACKGROUND OF THE INVENTION In the manufacture of yogurt, the management of lactic acid bacteria starter culture and its proper use are very important in determining the quality of yogurt. Lactic acid bacterium starter culture is roughly classified into two types, a bulk starter and a concentrated starter. The concentrated starter is easier to handle than a bulk starter, and is a desirable form of starter because it does not require advanced technology for management and use. Culture.
【０００３】このような濃縮スターターの製造には乳酸
菌の高濃度培養液を獲得する技術が必要である。従来よ
り乳酸菌の高濃度培養液を獲得する手法としては、乳酸
菌の培養物を遠心分離する方法が知られている。しか
し、この方法では菌体に対して物理的な応力が作用して
しまうため、菌体に与えるダメージが大きい上、高濃度
培養液を大量に得るためには、大規模な設備を必要とす
るために非効率的である。一方、培養装置にろ過装置部
を連結し、培養の進行によって蓄積する生育阻害物質を
ろ過によって培養系外に排出しながら新鮮な培地を供給
することにより、菌の生育に適した環境を維持しながら
培養を継続する方法、所謂連続培養法によっても菌体の
高濃度培養液を得ることができることが知られている。
この方法では、菌体に与えるダメージが小さくなり、し
かも小規模の装置で大量の高濃度培養液を得ることがで
きるという利点がある。例えば特公平６−６９３６７号
公報に開示される方法は、この連続培養法の一例であっ
て、乳酸菌（ビフィズス菌）を培養するに際し、培地の
糖濃度を１〜１．５重量％に調整し、培養により培地中
に生成した菌の阻害物質を培養槽に連通して配設したろ
過膜を介して除去し、該阻害物質を除去した培養液を培
養槽へ循環させると共に、上記濾過膜を介して除去され
た部分に相当する液量の新鮮な培地を培養槽へ補給する
方法が記載されている。また、この培養操作において、
培養開始から乳酸生成による阻害現象が現れる経過時間
（実施例では４時間程度）以降では、菌体の増殖に応じ
て培地供給速度を高める必要があることが明記されてい
る。しかしながら、一般的には、菌体が増殖すればする
ほど、濾過膜の目詰まりが生じ易くなり、その結果とし
て濾過量が少なくなって培地供給も滞る。つまり、この
方法にあるように、菌体の増殖に応じて培地供給速度を
高めるという手法を実現するには、膜面積を極度に広く
しておく必要があり、膜の導入コストを考慮すると必ず
しも実用的な方法とはいえない。[0003] The production of such a concentrated starter requires a technique for obtaining a highly concentrated culture solution of lactic acid bacteria. Conventionally, as a technique for obtaining a high-concentration culture solution of lactic acid bacteria, a method of centrifuging a culture of lactic acid bacteria is known. However, in this method, physical stress acts on the cells, so that the damage to the cells is large, and a large-scale facility is required to obtain a large amount of a high concentration culture solution. Inefficient because of On the other hand, by connecting the filtration device to the culture device and supplying a fresh medium while filtering out the growth inhibitory substances that accumulate as the culture progresses out of the culture system, an environment suitable for the growth of bacteria is maintained. It is known that a high-concentration culture solution of bacterial cells can be obtained also by a method of continuing culturing while performing the so-called continuous culture method.
This method has an advantage that damage to the cells is reduced and a large amount of a high concentration culture solution can be obtained with a small-scale device. For example, the method disclosed in Japanese Patent Publication No. 6-69367 is an example of this continuous culture method. In culturing lactic acid bacteria (Bifidobacterium), the sugar concentration of the medium is adjusted to 1 to 1.5% by weight. An inhibitor of bacteria produced in the culture medium by culturing is removed through a filtration membrane provided in communication with the culture tank, and the culture solution from which the inhibitor has been removed is circulated to the culture tank, and the filtration membrane is removed. A method of replenishing a culture tank with a fresh medium in a liquid amount corresponding to a portion removed through the medium is described. In this culture operation,
It is specified that after the lapse of time (about 4 hours in the example) from the start of culture when the inhibition phenomenon due to lactic acid production appears, it is necessary to increase the medium supply rate in accordance with the growth of the cells. However, in general, as the cells grow, the filter membrane is more likely to be clogged, and as a result, the amount of filtration is reduced and the supply of the culture medium is delayed. In other words, as described in this method, in order to realize a method of increasing the medium supply rate in accordance with the growth of cells, it is necessary to extremely increase the membrane area. It is not a practical method.
【０００４】[0004]
【発明が解決しようとする課題】代表的なヨーグルトス
ターター乳酸菌であるStreptococcus salivarius subs
p.thermophilus（ＳＴ菌）は、その生育に際して栄養要
求性が高く、しかも酸に対する耐性が低い。したがっ
て、ＳＴ菌の連続培養を行うためには必要な栄養成分を
潤沢に供給し、且つ代謝産物である乳酸を速やかに培養
系外へ排出する必要がある。このＳＴ菌の高濃度培養に
前述の方法を適用しようとすると、通常は上述の通り菌
体が増殖することで濾過膜の目詰まりが生じて濾過量は
少なくなり、培地供給速度は低下する。その結果、不要
物である乳酸が蓄積し易くなるので、菌体の増殖は容易
に停止してしまう。いってみれば、前述の方法では、極
度に膜面積を広くしない限り、短時間に且つ安定にＳＴ
菌の高濃度培養液を得ることはできない。そこで、本発
明者らは、このＳＴ菌を連続培養する条件について鋭意
検討の末、本発明を見出すに至った。SUMMARY OF THE INVENTION Streptococcus salivarius subs, a typical yogurt starter lactic acid bacterium
p.thermophilus (ST bacterium) has high auxotrophy during its growth and low acid resistance. Therefore, in order to continuously culture ST bacteria, it is necessary to supply necessary nutrient components abundantly and to rapidly discharge lactic acid, which is a metabolite, out of the culture system. When the above-mentioned method is applied to the high-concentration culture of this ST bacterium, usually, as described above, the bacterial cells proliferate, clogging of the filtration membrane occurs, the amount of filtration decreases, and the medium supply rate decreases. As a result, lactic acid, which is an unnecessary substance, easily accumulates, so that the growth of bacterial cells is easily stopped. In other words, in the above-described method, unless the film area is extremely widened, the ST can be stably performed in a short time.
A high concentration culture of the bacterium cannot be obtained. The inventors of the present invention have eagerly studied conditions for continuously culturing the ST bacterium and have found the present invention.
【０００５】[0005]
【課題を解決するための手段】本発明は、上記に鑑み提
案されたもので、ろ過培養装置により、大豆タンパク分
解物、酵母エキス、ラクトースを主体とした培地を用い
て、ヨーグルトスターター乳酸菌を連続培養する方法で
あって、ヨーグルトスターター乳酸菌種は、ストレプト
コッカス・サリバリウス・サブスピーシス・サーモフィ
ルス(Streptococcus salivarius subsp.thermophilus)
であって、培地中の酵母エキス濃度を０．５〜２．０％
とし、培養液量に対する１時間当たりのろ過液量の比
（希釈率）を１．０以上の一定値を維持するように培養
することを特徴とするヨーグルトスターター乳酸菌の高
濃度培養方法に関するものである。Means for Solving the Problems The present invention has been proposed in view of the above, and a method of continuously culturing a yogurt starter lactic acid bacterium using a culture medium mainly containing soybean protein decomposed products, yeast extract and lactose by a filtration culture apparatus. A method of culturing, the yogurt starter lactic acid bacteria species, Streptococcus salivarius subsp. Thermophilus (Streptococcus salivarius subsp.thermophilus)
The yeast extract concentration in the medium is 0.5 to 2.0%
A method for culturing a yogurt starter lactic acid bacterium at a high concentration, wherein the culture is performed so that the ratio of the amount of filtrate per hour to the amount of culture solution (dilution ratio) is maintained at a constant value of 1.0 or more. is there.
【０００６】また、本発明は、上記方法により得られた
高濃度培養液を濃縮スターターとして用いることにより
ヨーグルトを製造することを特徴とするヨーグルトの製
造方法をも提案するものである。[0006] The present invention also proposes a method for producing yogurt, wherein yogurt is produced by using a high-concentration culture solution obtained by the above method as a concentration starter.
【０００７】[0007]
【発明の実施の形態】上記菌種を連続培養するにあた
り、栄養成分の潤沢な供給を目的として、添加する培地
中の酵母エキス濃度を増量し、また培養液中の乳酸濃度
を低く抑えることを目的として、培養液量に対する１時
間当たりのろ過液量の比（以下、希釈率という）を高め
て培養することに着目し、その効果について確認した。
尚、希釈率は前記従来例のように経時的に変化させるも
のではなく一定値を維持するようにした。DESCRIPTION OF THE PREFERRED EMBODIMENTS In continuous cultivation of the above-mentioned bacterial species, it is necessary to increase the concentration of yeast extract in a medium to be added and to keep the concentration of lactic acid in a culture solution low in order to supply nutrient components in a sufficient manner. For the purpose, we focused on raising the ratio of the amount of the filtrate per hour to the amount of the culture solution (hereinafter referred to as dilution ratio) and cultured, and confirmed the effect thereof.
The dilution rate was not changed over time as in the above-mentioned conventional example, but was maintained at a constant value.
【０００８】菌株及び培地 使用菌株としてはストレプトコッカス・サリバリウス・
サブスピーシス・サーモフィルス(Streptococcus saliv
arius subsp.thermophilus) ＩＦＯ１３９５７株、ＺＥ
Ｒ６１４１０株（工業技術院生命工学工業技術研究所寄
託 寄託番号；ＦＥＲＭ Ｐ−１７７０６）の２株を用
い、使用培地としては下記表１に記載の通り、通常培
地、添加用培地の２種類を使用した。[0008] Strains and culture media used are Streptococcus salivarius
Subsp. Thermophilus (Streptococcus saliv
arius subsp. thermophilus) IFO13957 strain, ZE
Two strains of R61410 strain (Deposited by the National Institute of Bioscience and Biotechnology, Deposit No .; FERM P-17706) were used, and two types of media, a normal medium and an addition medium, were used as shown in Table 1 below. did.
【表１】 [Table 1]
【０００９】培養 培養装置の概観図を図１に示した。培養タンク１に４０
０mlの通常培地を送り込み、通常培地にて培養した前培
養物を２０ml接種して培養を開始した。培養温度は約３
５℃とし、ｐＨセンサー４を用いて培養液ｐＨのモニタ
リングを行い、ｐＨ調整液タンク５より２Ｎ−ＮａＯＨ
溶液を送り込むことで、ｐＨを調整しながら培養した。
培養３時間後から循環ポンプ２を用いて培養液を循環さ
せ、膜モジュール（孔径０．２μｍ、膜面積０．０４
m2、ろ過圧力０．２kgf/cm2）３を用いた培養液のろ過
を行った。ろ過の間、液量センサー６によって培養液量
をモニタリングしつつ、培養タンク１の内液量が４００
mlを維持するように、培地タンク７より添加用培地を培
養タンク１内に送り込むことで培養を継続した。このと
きの希釈率は最大３．０とした。培養開始より１２時間
経過した時点をもって培養を終了した。Culture FIG. 1 shows an overview of the culture apparatus. 40 in culture tank 1
The culture was started by injecting 0 ml of the normal medium and inoculating 20 ml of the preculture cultured in the normal medium. Culture temperature is about 3
The temperature was adjusted to 5 ° C., and the pH of the culture solution was monitored using the pH sensor 4.
The culture was performed while adjusting the pH by feeding the solution.
After 3 hours from the culture, the culture solution was circulated using the circulation pump 2 and the membrane module (pore diameter 0.2 μm, membrane area 0.04
The culture solution was filtered using m 2 and a filtration pressure of 0.2 kgf / cm 2 ) 3. During the filtration, the amount of the culture solution in the culture tank 1 is 400
The culture was continued by feeding the culture medium for addition from the culture tank 7 into the culture tank 1 so as to maintain the ml. The dilution rate at this time was set to 3.0 at the maximum. The culture was terminated when 12 hours had elapsed from the start of the culture.
【００１０】１．培地成分が菌の生育に及ぼす影響 ＩＦＯ１３９５７株について、添加用培地中の酵母エキ
ス濃度を変えて連続培養を行い、添加用培地中の酵母エ
キスの濃度が生育に及ぼす影響を見た。この結果を表２
に示した。尚、表２中の生菌数は、培養中に得られた最
高値を示し、おおむね培養９〜１１時間目における数値
となっている。[0010] 1. Effect of medium components on growth of bacteria For the IFO13957 strain, continuous culture was performed while changing the concentration of yeast extract in the medium for addition, and the effect of the concentration of yeast extract in the medium for addition on growth was observed. Table 2 shows the results.
It was shown to. In addition, the viable cell count in Table 2 shows the highest value obtained during the culturing, and is generally a numerical value at 9 to 11 hours after the culturing.
【表２】 表２にあるとおり、酵母エキス濃度を調整し、栄養成分
の適切な供給を行った場合（酵母エキス濃度０．５〜
２．０％）はそうでない場合と比べて生菌数を高くでき
ることがわかった。酵母エキス濃度が低すぎる（０．３
％）と成分の供給不足となるため生菌数が低くなり、逆
に高すぎる（３．０％）と酵母エキス中に含まれる塩分
及びその他の物質が菌の生育を阻害するために生菌数が
低くなるものと考えられる。[Table 2] As shown in Table 2, when the yeast extract concentration was adjusted and nutrient components were appropriately supplied (yeast extract concentration 0.5 to
2.0%) can increase the viable cell count as compared to the case where it is not. The yeast extract concentration is too low (0.3
%), The supply of components is insufficient, and the number of viable bacteria is low. On the contrary, if it is too high (3.0%), the salt and other substances contained in the yeast extract inhibit the growth of the bacteria. It is expected that the number will be lower.
【００１１】２．希釈率が菌の生育に及ぼす影響 次に、ＩＦＯ１３９５７株を用いて、希釈率を変えて同
様に連続培養を行い、菌の生育を比較した。この結果を
表３に示した。また、前項の結果より、添加用培地の酵
母エキス濃度は１．０％として培養を行った。尚、表３
中の生菌数は、培養中に得られた最高値を示し、おおむ
ね培養９〜１１時間目における数値となっている。2. Influence of Dilution Rate on Growth of Bacteria Next, continuous culture was similarly performed using the IFO13957 strain at different dilution rates to compare the growth of the bacteria. The results are shown in Table 3. From the results in the preceding section, the culture was performed with the yeast extract concentration of the addition medium being 1.0%. Table 3
The number of viable bacteria in the medium indicates the highest value obtained during the culture, and is generally a numerical value at 9 to 11 hours after the culture.
【表３】 表３に示すとおり、希釈率が高くなるに伴い、生菌数は
増加した。本発明のように、濾過開始当初から希釈率を
高くして培養することで、培養液中の乳酸濃度が低く抑
えられ、菌にとっても生育に適した条件が長時間維持さ
れたことから、生菌数が高くなったものと考えられる。
乳酸菌にとって望ましい生育条件を提供する観点に立て
ば、希釈率は高ければ高い程良く、また、ある時間帯の
み希釈率を高くしても大した効果は見込めず、継続的に
希釈率の高い状態（１．０以上の一定値）を維持するこ
とが必要である。[Table 3] As shown in Table 3, as the dilution ratio increased, the number of viable bacteria increased. As in the present invention, by culturing at a high dilution ratio from the beginning of filtration, the lactic acid concentration in the culture solution is kept low, and the conditions suitable for the growth of bacteria are maintained for a long time. It is considered that the number of bacteria increased.
From the standpoint of providing desirable growth conditions for lactic acid bacteria, the higher the dilution rate, the better, and even if the dilution rate is increased only during a certain period of time, no significant effect can be expected, and the dilution rate is continuously high. (A constant value of 1.0 or more) must be maintained.
【００１２】このようにＳＴ菌の高濃度培養において、
酵母エキスの濃度を０．５〜２．０％とし、希釈率を
１．０以上の一定値を維持するように設定して培養を行
うことで生菌数の多い高濃度培養液を得ることが可能で
あるとわかった。これらのことは、栄養素の供給や不要
物の除去を速やかに行うといった、菌の生育環境を直接
に改善できる条件を設定することによって生菌数を高め
られることを示している。Thus, in the high concentration culture of ST bacterium,
A high-concentration culture solution with a large number of viable bacteria is obtained by culturing while setting the concentration of the yeast extract to 0.5 to 2.0% and maintaining the dilution ratio at a constant value of 1.0 or more. Proved possible. These facts indicate that the number of viable bacteria can be increased by setting conditions that can directly improve the growth environment of the bacteria, such as promptly supplying nutrients and removing unnecessary substances.
【００１３】また、上述の実験において、培地（通常培
地及び添加用培地）中のラクトース濃度を２．０％とし
たが、それより低い濃度、例えば前記従来例に見られる
ような１．０％や１．５％では培養時間が著しく長大と
なるため、その分設備稼動時間並びに消費原材料が必要
となり、コストがかかるものとなる。言い換えれば培地
中のラクトース濃度を少なくとも２．０％とすることに
より培養時間を最大でも１２時間に抑えることができ、
製造コストを軽減でき、就労（管理）時間の短縮にも貢
献するものとなる。In the above-mentioned experiments, the lactose concentration in the medium (normal medium and medium for addition) was set to 2.0%, but lower than that, for example, 1.0% as in the conventional example. At 1.5%, the cultivation time becomes extremely long, so that the equipment operation time and the raw materials to be consumed are required, and the cost is high. In other words, by setting the lactose concentration in the medium to at least 2.0%, the culturing time can be suppressed to at most 12 hours,
Manufacturing costs can be reduced, and work (management) time can be reduced.
【００１４】さらに、上述の実験においては、ろ過膜と
してセラミック膜を用いることによりろ過膜と循環ライ
ンを加熱殺菌できるようにしたが、膜の種類はこれに限
定されず、どのような膜を用いても良く、その他の構成
においても公知の連続培養法に基づいて同様に行うこと
ができる。Further, in the above-mentioned experiments, the filtration membrane and the circulation line can be heated and sterilized by using a ceramic membrane as the filtration membrane. However, the type of the membrane is not limited to this, and any type of membrane can be used. Alternatively, other configurations can be similarly performed based on a known continuous culture method.
【００１５】こうした本発明の高濃度培養方法により、
ＳＴ菌の高濃度培養液が得られることがわかったので、
次に取得した高濃度培養液を用いてヨーグルトを調製
し、バルクスターターによるものを比較した。According to the high concentration culture method of the present invention,
Since it was found that a high concentration culture of ST bacteria was obtained,
Next, yogurt was prepared using the obtained high-concentration culture solution, and the results obtained by using a bulk starter were compared.
【００１６】ヨーグルト調製に適したＳＴ菌ＺＥＲ６１
４１０株を用いて、前述の手法に沿って連続培養を行
い、高濃度培養液を得た。その際の添加用培地中の酵母
エキス濃度は０．５％、希釈率は最大３．０とした。得
られた培養液中の生菌数は３．０×１０10cfu/mlであっ
た。高濃度培養液中の生菌数が本株のバルクスターター
の約１５倍であったため、バルクスターターの１／１５
の量を濃縮スターターとして接種してヨーグルトを調製
した。調製工程は以下の通りとした。生乳、脱脂粉乳、
調合水を配合したはっ酵乳ミックス（無脂乳固形分９．
５％、乳脂肪分３．１％）を、プレート殺菌機により殺
菌（１２０℃、２秒）および均質（１５Ｍｐａ）し、４
３℃まで冷却する。アシドフィルス菌バルクスターター
をミックスに対して２重量％接種したのち、ミックスを
２等分する。一方には上述により得られたＺＥＲ６１４
１０株の高濃度培養液を０．１３重量％接種し、もう一
方には同株のバルクスターターを２重量％接種する。そ
れぞれを１００ｇずつ容器に数個充填し、４１℃にて発
酵させる。乳酸酸度がしかるべき値に達したら発酵を終
了し、５℃冷蔵庫内にて冷却を行う。このようにして、
ヨーグルト製造における、本発明による高濃度培養液
と、従来のバルクスターターとの比較を行った。ST strain ZER61 suitable for yogurt preparation
Using the 410 strains, continuous culturing was carried out in accordance with the above-mentioned method to obtain a high concentration culture solution. At that time, the yeast extract concentration in the medium for addition was 0.5%, and the dilution ratio was 3.0 at the maximum. The viable cell count in the obtained culture solution was 3.0 × 10 10 cfu / ml. Since the viable cell count in the high concentration culture was about 15 times that of the bulk starter of this strain, it was 1/15 that of the bulk starter.
Was inoculated as a concentration starter to prepare yogurt. The preparation process was as follows. Raw milk, skim milk powder,
Fermented milk mix (mixed with non-fat milk 9.
5%, milk fat content 3.1%) was sterilized (120 ° C., 2 seconds) and homogenized (15 Mpa) using a plate sterilizer.
Cool to 3 ° C. After inoculating 2 wt% of the acidophilus bulk starter to the mix, the mix is divided into two equal parts. On the one hand, ZER614 obtained as described above
0.13% by weight of 10 strains of a high concentration culture is inoculated, and the other is inoculated with 2% by weight of a bulk starter of the same strain. 100 g of each is filled in a container and fermented at 41 ° C. When the lactic acidity reaches an appropriate value, the fermentation is terminated and cooling is performed in a refrigerator at 5 ° C. In this way,
A comparison was made between a high-concentration culture solution according to the present invention and a conventional bulk starter in yogurt production.
【００１７】上述の工程により調製されたヨーグルトの
品質を表４に示した。Table 4 shows the quality of the yogurt prepared by the above-described process.
【表４】 表４に示すとおり、前記本発明の培養方法によって得ら
れた高濃度培養液を用いて調製されたヨーグルトの品質
は、バルクスターターによるものと比べて遜色ないもの
であった。このことは、ＳＴ菌の高濃度培養液が、バル
クスターターに代替して利用可能であることを示すもの
であり、代替することで、ヨーグルト製造時のスタータ
ー使用量を低減でき、且つスターター管理が容易にな
る。尚、本実施例においては、所謂プレーンヨーグルト
と呼ばれるものの製造について本発明による高濃度培養
液の効果を説明したが、本発明の用途はこれに限定され
ず、ソフトヨーグルト、ドリンクヨーグルト等、あらゆ
る種類のヨーグルト製造に利用できる。[Table 4] As shown in Table 4, the quality of the yogurt prepared using the high-concentration culture solution obtained by the culture method of the present invention was comparable to that of the bulk starter. This indicates that a high concentration culture solution of ST bacterium can be used instead of a bulk starter, and by using it, the amount of starter used during yogurt production can be reduced, and starter management can be reduced. It will be easier. In this example, the effect of the high-concentration culture solution according to the present invention was described for the production of what is called plain yogurt, but the use of the present invention is not limited thereto, and soft yogurt, drink yogurt, etc. Can be used for yogurt production.
【００１８】[0018]
【発明の効果】以上説明したように本発明の高濃度培養
方法により、ヨーグルトスターター乳酸菌として広く利
用されているストレプトコッカス・サリバリウス・サブ
スピーシス・サーモフィルスの高濃度培養液を短時間に
且つ安定して、効率よく得ることができる。As described above, according to the high-concentration culture method of the present invention, a high-concentration culture of Streptococcus salivarius subsp. Thermophilus, which is widely used as a yogurt starter lactic acid bacterium, can be prepared in a short time and stably. It can be obtained efficiently.
【００１９】また、得られた高濃度培養液は、ヨーグル
ト製造に適しており、バルクスターターと代替すること
でヨーグルト製造時のスターター使用量を低減でき、且
つスターター管理が容易になる。The obtained high-concentration culture solution is suitable for producing yogurt, and by replacing it with a bulk starter, the amount of starter used in producing yogurt can be reduced, and the starter can be easily managed.
【図１】本発明に使用するろ過培養装置の一例を示す概
略図である。FIG. 1 is a schematic view showing an example of a filtration culture device used in the present invention.
１ 培養タンク ２ 循環ポンプ ３ セラミック膜モジュール ４ ｐＨセンサー ５ ｐＨ調整液タンク ６ 液量センサー ７ 培地タンク DESCRIPTION OF SYMBOLS 1 Culture tank 2 Circulation pump 3 Ceramic membrane module 4 pH sensor 5 pH adjustment liquid tank 6 Liquid quantity sensor 7 Culture medium tank
───────────────────────────────────────────────────── フロントページの続き (72)発明者 井澤 登 埼玉県川越市的場1535番地 全国酪農業協 同組合連合会乳業部乳業開発研究所内 (72)発明者 西川 靖之 埼玉県川越市的場1535番地 全国酪農業協 同組合連合会乳業部乳業開発研究所内 Ｆターム(参考） 4B001 AC31 BC14 EC99 4B065 AA49X BB29 BC10 BC13 BC20 BC21 CA42 ──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Noboru Izawa 1535 Matoba, Kawagoe-shi, Saitama Japan Dairy Agricultural Cooperative Federation Dairy Development Research Institute (72) Inventor Yasuyuki Nishikawa 1535 Matoba, Kawagoe-shi, Saitama Address National Dairy Agriculture Cooperative Federation Dairy Development Laboratory Dairy Development Laboratory F-term (Reference) 4B001 AC31 BC14 EC99 4B065 AA49X BB29 BC10 BC13 BC20 BC21 CA42
Claims (2)
物、酵母エキス、ラクトースを主体とした培地を用い
て、ヨーグルトスターター乳酸菌を連続培養する方法で
あって、 ヨーグルトスターター乳酸菌種は、ストレプトコッカス
・サリバリウス・サブスピーシス・サーモフィルス(Str
eptococcus salivarius subsp.thermophilus)であっ
て、 培地中の酵母エキス濃度を０．５〜２．０％とし、培養
液量に対する１時間当たりのろ過液量の比（希釈率）を
１．０以上の一定値を維持するように培養することを特
徴とするヨーグルトスターター乳酸菌の高濃度培養方
法。1. A method for continuously culturing yogurt starter lactic acid bacteria using a medium mainly comprising soybean protein decomposed products, yeast extract, and lactose by a filtration culture apparatus, wherein the yogurt starter lactic acid bacteria species are Streptococcus salivarius. Subspecies Thermofils (Str
eptococcus salivarius subsp. thermophilus), wherein the yeast extract concentration in the medium is 0.5 to 2.0%, and the ratio of the amount of filtrate per hour to the amount of culture solution (dilution ratio) is 1.0 or more. A high-concentration culture method for lactic acid bacteria of yogurt starter, which comprises culturing to maintain a constant value.
縮スターターとして用いることによりヨーグルトを製造
することを特徴とするヨーグルトの製造方法。2. A method for producing yogurt, comprising using the high-concentration culture solution obtained in claim 1 as a concentration starter to produce yogurt.
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|---|---|---|---|
| JP2000028246A JP2001211878A (en) | 2000-02-04 | 2000-02-04 | Method of culturing yogurt starter lactobacillus in high concentration, and method of producing yogurt from the obtained high concentration culture solution |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2000028246A JP2001211878A (en) | 2000-02-04 | 2000-02-04 | Method of culturing yogurt starter lactobacillus in high concentration, and method of producing yogurt from the obtained high concentration culture solution |
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| Publication Number | Publication Date |
|---|---|
| JP2001211878A true JP2001211878A (en) | 2001-08-07 |
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ID=18553682
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|---|---|---|---|
| JP2000028246A Withdrawn JP2001211878A (en) | 2000-02-04 | 2000-02-04 | Method of culturing yogurt starter lactobacillus in high concentration, and method of producing yogurt from the obtained high concentration culture solution |
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| JP (1) | JP2001211878A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300900A (en) * | 2002-04-11 | 2003-10-21 | Lotte Co Ltd | Calcium absorption promoter, promoter of crystal growth of calcium phosphate, and food and drink and fodder containing them |
| WO2004005493A1 (en) * | 2002-07-09 | 2004-01-15 | Baxter International, Inc. | Animal protein free media for cultivation of cells |
| JP2006238706A (en) * | 2005-02-28 | 2006-09-14 | Kanehide Bio Kk | Culturing medium of lactobacillus bifidus, method for culturing lactobacillus bifidus by using the same and food added with lactobacillus bifidus cultured by the method |
| WO2007097260A1 (en) | 2006-02-24 | 2007-08-30 | Toray Industries, Inc. | Method of producing chemical product and continuous fermentation apparatus |
| JP2014187948A (en) * | 2013-03-27 | 2014-10-06 | Sunstar Inc | Crushed fruit product with suppressed change in color tone, and manufacturing method thereof |
-
2000
- 2000-02-04 JP JP2000028246A patent/JP2001211878A/en not_active Withdrawn
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003300900A (en) * | 2002-04-11 | 2003-10-21 | Lotte Co Ltd | Calcium absorption promoter, promoter of crystal growth of calcium phosphate, and food and drink and fodder containing them |
| WO2004005493A1 (en) * | 2002-07-09 | 2004-01-15 | Baxter International, Inc. | Animal protein free media for cultivation of cells |
| AU2003249990B2 (en) * | 2002-07-09 | 2007-06-28 | Takeda Pharmaceutical Company Limited | Animal protein free media for cultivation of cells |
| EP2287288A1 (en) * | 2002-07-09 | 2011-02-23 | Baxter International Inc. | Animal protein free media for cultivation of cells |
| US7955833B2 (en) | 2002-07-09 | 2011-06-07 | Baxter International Inc. | Animal protein free media for cultivation of cells |
| US8524497B2 (en) | 2002-07-09 | 2013-09-03 | Baxter International Inc. | Animal protein free media for cultivation of cells |
| US9163211B2 (en) | 2002-07-09 | 2015-10-20 | Baxter International Inc. | Animal protein free media for cultivation of cells |
| JP2006238706A (en) * | 2005-02-28 | 2006-09-14 | Kanehide Bio Kk | Culturing medium of lactobacillus bifidus, method for culturing lactobacillus bifidus by using the same and food added with lactobacillus bifidus cultured by the method |
| WO2007097260A1 (en) | 2006-02-24 | 2007-08-30 | Toray Industries, Inc. | Method of producing chemical product and continuous fermentation apparatus |
| JP2014187948A (en) * | 2013-03-27 | 2014-10-06 | Sunstar Inc | Crushed fruit product with suppressed change in color tone, and manufacturing method thereof |
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