JP2001122707A - Formulation for culturing laver and method for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an inexpensive acidic treating agent which is high in an effect for exterminating reddish-brown scab fungi, diatom and so on. SOLUTION: This method for producing a formulation for culturing layer, characterized by wet-crushing at least one kind of hydrophobic organic acid selected from the group consisting of fumaric acid, adipic acid and succinic acid together with water or an acidic solution.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a method for producing a nori cultivation preparation capable of stably dispersing hydrophobic organic acids such as fumaric acid, adipic acid and succinic acid in an aqueous solution, and a method for producing the same. The present invention relates to a formulation for seaweed cultivation that is effective for controlling red rot fungi and diatoms such as diatoms, and that has excellent solubility when used after dilution.

[0002]

2. Description of the Related Art In the cultivation of laver, the occurrence of red rot fungi degrades the quality of the laver, and the production of the laver may be completely absent. For this reason, drying is generally performed as a method for preventing and controlling diseases in laver culture. This is a method of once removing the laver net from the seawater and drying it.
This method is not very effective at the expense of great effort and time. As another method, there is a so-called acid treatment method in which red rot bacteria and diatoms are eliminated by immersion treatment in an organic acid solution such as citric acid or malic acid. For example, when removing red rot bacteria, 1 to 2% of citric acid
It is common to treat the solution for 5 to 10 minutes. However, the conventional acid treatment method has a disadvantage that it takes too much time to treat red rot bacteria. The disease, which is called red rot, occurs rapidly when the laver foliage grows and finally becomes harvestable, spreads to all fishing grounds in a few days, and dies and leaves the foliate. The damage is devastating.

[0003] The control of this red rot fungus involves the control and prevention of algae and diseases immersed in a treatment liquid containing 0.3 to 5.0% by weight of citric acid and having a pH of 1.0 to 6.0. Nori cultivation method (Japanese Patent Publication No. 60-13647) and pH 1.0 or more by adding inorganic acids such as hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid and the like.
A seaweed cultivation method (Japanese Patent Publication No. 60-13648) for controlling and preventing harmful alga and disease, which is referred to as 4.0, has been proposed. Further, Japanese Patent Application Laid-Open No. 60-21950 discloses a preventive effect against red rot and a diatom control effect of a nori cultivation fertilizer containing phytic acid or a salt thereof as an active ingredient. JP-A-57-8722 discloses a nori cultivation method in which a nori net is treated with a treatment solution containing phosphoric acid or a salt thereof to remove and prevent diseases such as diatoms and red rot. .

In order to control red rot fungi, as described above, an organic or inorganic acid which has a property of being liquid at room temperature or of being solid but immediately dissolving in seawater is used. Acids that are liquid at room temperature include, for example, phytic acid, acetic acid, lactic acid, phosphoric acid, hydrochloric acid, and nitric acid. Acids that are solid at room temperature but easily soluble in seawater include citric acid and malic acid. Organic acids such as fumaric acid and adipic acid, on the other hand, have the advantage of being relatively inexpensive, but have poor solubility in water (seawater), so citric acid,
The fact is that malic acid is used.

In the case of using fumaric acid, an amount of fumaric acid exceeding a saturated amount is present in a treatment liquid such as seawater contained in a treatment tank in a state covered by a water-permeable covering means. And a method of killing green alga (Japanese Patent Publication No. 3-47810) and fumaric acid and / or fumaric acid and sodium carbonate, sodium hydrogencarbonate, potassium carbonate and potassium hydrogencarbonate. A disinfectant for algae and bacteria, wherein the disinfectant containing at least one carbonate is converted to monosodium fumarate and / or monopotassium fumarate when dissolved in water or seawater (Japanese Patent Application Laid-Open No. No. 2-25404). The latter kills bacteria with fumarate. However, actually, the seawater temperature during the laver cultivation period is 10 to 20 ° C., and even if 0.1 to 0.3% of fumaric acid is added to the seawater, it floats or precipitates on the sea surface and hardly dissolves. . In order to dissolve fumaric acid, it must be heated and dissolved, and in practice it cannot be used in fishing grounds.

On the other hand, when algae such as Aonori and diatoms, which are generated in laver culture, adhere to the laver net and proliferate, the growth of the laver is hindered, or the laver is mixed with the product to lower the price of the product. Since diatoms adhere to laver nets and laver leaves, nori cannot absorb nutrients in seawater,
If diatoms are attached more, they will rot. In addition, when laver to which diatoms are attached is made into a product, the product has a bad taste and becomes a dull product, and the price is lowered. Therefore, Aonori,
Algae such as diatoms must be cultivated in seaweed while constantly exterminating them.

[0007] In general, laver producers collect 200 to 1000 laver nets on average. Current processing agents require a processing time of 5 minutes or more per screen, so that 30 to 40 screens can be operated in one day. Conventionally, a commercially available acid treatment agent has been used after being diluted 100 to 200 times with seawater. However, in recent years, the culture has changed to a large-scale culture of 500 or more, and it is necessary to shorten the time for acid treatment. A moatboat was developed to do. Since the time during which the laver net is immersed in the treatment liquid at this time is as short as 10 to 20 seconds, a normal acid treatment agent has to be used at a high concentration of 10 to 20 times. As a result, the amount of the acid treating agent to be used is increased, which causes a problem of increasing the load on the sea and increasing costs.

Further, the above-mentioned fumaric acid, adipic acid and succinic acid are recognized as food additives and are safe and relatively inexpensive, but have the disadvantage that they are hydrophobic organic acids and their solubility is low. Used as a main component of the acid treatment agent for sewage treatment.

[0009]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide an inexpensive acid treating agent utilizing a hydrophobic organic acid, which has a high effect of controlling red rot bacteria and diatoms and the like, and is inexpensive.

[0010]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies to solve the above-mentioned problems, and as a result, the hydrophobic organic acids fumaric acid, adipic acid and succinic acid have been replaced with specific organic acids. Or, by wet grinding with a solution of an inorganic acid, it was possible to stabilize the dispersion in the solution, and when it was diluted to near the saturated concentration of fumaric acid, it was found that it dissolved immediately, and the present invention was completed. .

That is, the present invention provides the following (1) to (5)
Exists. (1) A method for producing a formulation for laver cultivation, comprising wet-grinding at least one hydrophobic organic acid selected from the group consisting of fumaric acid, adipic acid and succinic acid together with water or an acidic solution. (2) The method for producing a formulation for laver culture according to the above (1), wherein the acidic solution is a solution of one or more acids selected from lactic acid, acetic acid, formic acid, propionic acid, hydrochloric acid, phosphoric acid, nitric acid and sulfuric acid. (3) The method for producing a formulation for laver culture according to the above (1) or (2), wherein the hydrophobic organic acid is wet-pulverized to a particle size of 200 μm or less. (4) The method for producing a formulation for laver cultivation according to any one of (1) to (3), wherein partially saponified polyvinyl alcohol or magnesium hydroxide having a particle size of 10 μm or less is used as the dispersion stabilizer. (5) A formulation for laver cultivation obtained by the method according to any one of (1) to (4).

[0012]

DETAILED DESCRIPTION OF THE INVENTION The process of the present invention comprises the steps of:
It is carried out by wet grinding with water or an acidic solution.

[0013] The hydrophobic organic acid used in the present invention is selected from fumaric acid, adipic acid and succinic acid. These hydrophobic organic acids have low solubility in water and are very difficult to use as a formulation for laver cultivation. As shown in Table 1, the solubility is extremely low as compared with citric acid and malic acid. . The numerical values in Table 1 are the solubility in water, and the solubility is further reduced in the case of seawater.

[0014]

[Table 1]

In particular, fumaric acid and adipic acid have a considerably stronger red rot control effect than citric acid and malic acid (see Test 1 described later) and are relatively inexpensive. However, since it has almost no hygroscopicity and a small specific gravity, when added to water, it floats on the water surface and does not readily dissolve. Therefore, there is no other way to dissolve in a short time except by heating and dissolving. For these reasons, fumaric acid or adipic acid is not used as a formulation for laver culture, and expensive citric acid or malic acid is currently used.

In the production method of the present invention, a hydrophobic organic acid is wet-pulverized together with water or an acidic solution. This allows
Hydrophobic organic acid can be dispersed and stabilized, and it is possible to dissolve hydrophobic organic acid instantaneously even if the preparation is diluted to a concentration that does not dissolve unless heated. It has become possible to provide a red rot pesticide.

As the acid used in the acidic solution used in the wet pulverization of the present invention, for example, a liquid at room temperature such as lactic acid, acetic acid, formic acid, propionic acid, hydrochloric acid, phosphoric acid, nitric acid and sulfuric acid is preferable. The concentration of the acidic solution is 10-100% by weight
(Hereinafter simply referred to as “%”) is preferable.

The wet pulverization can be carried out, for example, by an apparatus such as a wet pulverizer [Mascorider MKZA10-20J (manufactured by Masuko Sangyo)]. The mixing ratio of the hydrophobic organic acid to the acidic solution at the time of pulverization is preferably, for example, about 1: 0.1 to 20.

It is preferable that the wet pulverization is performed until the particle diameter of the hydrophobic organic acid becomes 200 μm or less.

In the present invention, the dispersion stability can be further improved by adding partially saponified polyvinyl alcohol or magnesium hydroxide having an average particle size of 10 μm or less as a dispersion stabilizer. Here, as partially saponified polyvinyl alcohol, Gohselan L-0302
(Degree of saponification: 40 to 50 mol%), Gohselan L-326
6 (86.5 to 89.5 mol% saponification) {manufactured by Nippon Synthetic Chemical Industry Co., Ltd.}, and as the magnesium hydroxide having an average particle size of 10 μm or less, Kisuma 2 (average particle size 1 μm; manufactured by Kyowa Chemical Industry Co., Ltd.) ) And the like.

After the wet pulverization, the nori cultivation preparation of the present invention is obtained in the form of, for example, a liquid (preferably a highly viscous liquid). Also, if necessary, for example, amino acids, ammonium nitrate,
It is also possible to add optional components such as fertilizer components such as salt and salt, sodium nitrate, sodium phosphate, ammonium phosphate and phytic acid.

The preparation for laver cultivation produced as described above has good dispersion stability, can be easily mixed even when stored for a long time, has a fast solubility in water or seawater, and has a hydrophobic organic property. Due to the action of the acid, it has a high control effect on algae such as red rot bacteria and diatoms.

[0023]

EXAMPLES Next, the present invention will be described in more detail with reference to examples and the like, but the present invention is not limited thereto.

Test 1 Fumaric acid, adipic acid, succinic acid, citric acid and malic acid were dissolved in seawater at 10 ° C. so as to be 0.1%, 0.2% and 0.3%, respectively. A red rot fungus-infected laver leaf body was put in the prepared solution at 10 ° C. for a certain period of time (10 seconds, 20 seconds,
··· 60 seconds, 2 minutes, 3 minutes,... 10 minutes) After immersion treatment, the cells were washed with seawater, and allowed to stand still for 24 hours to investigate the effect of controlling red rot bacteria. Since fumaric acid, adipic acid and succinic acid hardly dissolved in seawater at 10 ° C.,
After heating and dissolving at 0 ° C., it was cooled to 10 ° C. before use. Table 2 shows the results.

[0025]

[Table 2]

Test 2 After adding and mixing 300 g of fumaric acid to 700 g of water, the mixture was wet-pulverized using a wet pulverizer [Mascolider MKZA10-20J (manufactured by Masuko Sangyo)] so as to have the average particle sizes shown in Table 3. Easy to mix with the next day and one month after dispersion
And the solubility of fumaric acid at the time of 100-fold dilution in seawater was investigated (Examples 1 to 5). As a comparison, fumaric acid of each particle size was added to 30% and mixed with stirring (Comparative Examples 1 to 3).
5). The results are shown in Table 3.

[0027]

[Table 3]

As can be seen from Test 2, 200 fumaric acid was added.
By wet pulverization to a size of not more than μm, the dispersion stability is improved, and mixing can be easily performed even when stored for a long time.
As in the comparative example, simply dispersing fumaric acid that has been dry-ground to 100 μm or less in water simply separates it, and in long-term storage, fumaric acid aggregates to a large particle size, and even if mixed, There is no dispersion. Further, by wet pulverization, it can be dissolved immediately even when diluted to a fumaric acid concentration of 0.3%, which is not dissolved unless heated, but a dispersion in which dry pulverized fumaric acid is dispersed does not dissolve at all. Most of the seaweed during the nori cultivation period has a water temperature of 8 to 15 ° C, and when diluted and dissolved, it cannot be used in a fishing ground unless it is a preparation that is immediately dissolved in seawater at 8 to 15 ° C.

Test 3 After the acidic solutions of Examples 6 to 17 shown in Table 4 were prepared, fumaric acid, adipic acid, succinic acid and a dispersion stabilizer were added and mixed. An acid wet pulverized product was obtained, and the product stability and solubility upon dilution were investigated. In addition, 10 liters of red rot-infected nori leaf bodies were added to a 100-fold diluted seawater solution.
C for a certain time (5 seconds, 10 seconds, 15 seconds, 20 seconds, ...)
··· 180 seconds) After immersion treatment, the cells were washed with seawater, and allowed to stand still for 24 hours to investigate the effect of controlling red rot bacteria. Table 5 shows the results.

[0030]

[Table 4]

[0031]

[Table 5]

Test 3 examines the dispersion state, solubility, and bactericidal activity of fumaric acid, adipic acid, and succinic acid when wet-ground in an acidic solution. As in Test 2, the dispersion is stable even when wet-pulverized with an acidic solution, and the solubility of the hydrophobic organic acid is good. When partially saponified polyvinyl alcohol and magnesium hydroxide are used as the dispersion stabilizer, the dispersion stability is further improved. In addition, the effect of controlling red rot bacteria can be reduced to 40 seconds or less, and the noxious effect of nori is 3 times.
Since it did not occur even in minutes, it was found that red rot fungi can be safely and quickly eliminated.

Test 4 Fumaric acid, adipic acid, succinic acid, citric acid and malic acid were dissolved in seawater at 10 ° C. so as to be 0.1%, 0.2% and 0.3%, respectively. After immersing the dried seaweed leaf attached to diatom (Licumophora) in the prepared solution at 10 ° C. for a certain period of time (1 minute, 2 minutes, 3 minutes, 5 minutes, 8 minutes, and 10 minutes), washed with seawater, and then day and night The culture was allowed to stand still, and the diatom control effect was investigated. Since fumaric acid, adipic acid and succinic acid hardly dissolved in seawater at 10 ° C.,
After heating and dissolving at 50 ° C., it was cooled to 10 ° C. before use.
Table 6 shows the results.

[0034]

[Table 6]

Test 5 Examples 6, 7, 8, 9, 10, 11, 1 obtained in Test 3
Nori leaf (Licumophora, Tabularia) -attached laver leaf was immersed in the formulations 5, 16, and 17 at 10 ° C for a certain period of time (30 seconds, 40 seconds, 1 minute, 2 minutes, 3 minutes).
After washing with seawater, the culture was allowed to stand still all day and night to examine the diatom control effect. Table 7 shows the results.

[0036]

[Table 7]

[0037]

The hydrophobic organic acid such as fumaric acid, which has little hygroscopicity and usually floats on the water surface and does not dissolve, is wet-pulverized so that it can be dissolved immediately even if diluted to a concentration that does not dissolve alone. It is possible to provide an inexpensive and safe fast-dissolving preparation for laver cultivation.

Claims (5)

[Claims] 1. A method for producing a formulation for laver cultivation, comprising wet-milling at least one hydrophobic organic acid selected from the group consisting of fumaric acid, adipic acid and succinic acid together with water or an acidic solution. 2. The method according to claim 1, wherein the acidic solution is selected from lactic acid, acetic acid, formic acid, propionic acid, hydrochloric acid, phosphoric acid, nitric acid and sulfuric acid.
The method for producing a formulation for laver cultivation according to claim 1, which is a solution of at least one kind of acid. 3. The method for producing a formulation for laver culture according to claim 1, wherein the hydrophobic organic acid is wet-pulverized to a particle size of 200 μm or less. 4. The nori cultivation preparation according to claim 1, wherein partially saponified polyvinyl alcohol or magnesium hydroxide having a particle size of 10 μm or less is used as the dispersion stabilizer. Manufacturing method. 5. A preparation for laver cultivation obtained by the method according to any one of claims 1 to 4.