JP2001120252A - Method for culturing red nonsulfur bacterium and low- smell wastewater treatment equipment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an equipment for culturing a red nonsulfur bacterium, capable of efficiently producing an aqueous solution containing the red nonsulfur bacterium. SOLUTION: This equipment for culturing the red nonsulfur bacterium is equipped with an organic acid formation tank for forming a volatile lower fatty acid from a high- concentration organic aqueous solution and a culture tank for culturing the red nonsulfur bacterium by using the volatile lower fatty acid as a substrate formed in the organic acid formation tank. The equipment is provided with a waste liquor supply means for supplying the high-concentration organic aqueous solution to the organic acid formation tank, a means for removing a smell of the organic acid formation tank, capable of removing a smell occurring in the organic acid formation tank, an organic acid supply means for supplying an aqueous solution containing the volatile lower fatty acid formed in the organic acid formation tank, an organic acid dilution means for diluting the volatile lower fatty acid supplied to the culture tank, an oxygen supply means for maintaining the culture tank in an aerobic atmosphere, a culture tank smell removing means for removing a smell occurring in the culture tank and a bacterium supply means for supplying the aqueous solution containing the red nonsulfur bacterium proliferated in the culture tank to a demand side.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a wastewater treatment system for treating wastewater generated in a food treatment process or the like, which efficiently removes odor from wastewater with bad odor and improves the environment of wastewater treatment equipment. The present invention relates to a low-odor wastewater treatment device that removes odors, and a culture device that supplies red non-sulfur bacteria to cause the low-odor wastewater treatment device to deodorize.

[0002]

2. Description of the Related Art Wastewater generated in food manufacturing factories, such as dairy wastewater, generally contains a high concentration of BOD.
It is generally purified and discharged by biological treatment such as the activated sludge method. However, when treating such wastewater, raw water adjustment tanks, aeration tanks, excess sludge tanks, scum pits,
Often, bad odors were generated in dewatered cake storage tanks and the like. For this reason, conventionally, as means to suppress the generation of offensive odors in wastewater treatment equipment, addition of deodorants, addition of odor-decomposing bacteria, adsorption and removal of odor gases by adsorbents, oxidative decomposition of odor gases by ozone, etc. Various methods have been proposed, such as biodegradation by contact with odorous gas.

[0003] Among the methods by adding a deodorant,
The method of decomposing an odor component by reacting it with a deodorant may not be sufficient for some odor components, and it is necessary to always have an excess of deodorant for the odor component. Therefore, it is inevitable that the cost will increase. On the other hand, the method of masking the odor component with the fragrance also has the disadvantage that the selection of an appropriate fragrance is not easy and the supply of the fragrance must be constantly continued, which also increases the cost. Moreover, these methods have a drawback that they are not sufficiently effective against odors generated in wastewater treatment facilities of food manufacturing plants.

[0004] Decomposing bacteria having natural minerals, activated carbon, wood chips or the like as a carrier and organic matter-decomposing bacteria adhered to them are commercially available, but they are inferior in odor removal and extremely slow-acting. There is a disadvantage of high cost.

[0005] Furthermore, the method of sucking odor gas by sealing a device serving as an odor generating source and adsorbing, oxidatively decomposing, or biodegrading requires a large-scale structure such as a closed structure, a gas suction facility, and a gas deodorizing facility. In addition to this, there is a problem that capital investment is increased, and the cost and labor for maintenance of the equipment are required.

Here, Japanese Patent Application Laid-Open No. 54-118648 proposes a method for preventing offensive odor using photosynthetic bacteria such as red non-sulfur bacteria. This method is an excellent technique that has the advantages of relatively low cost, efficient odor prevention, and long-lasting effects.

However, it is easy to cultivate a small amount of red non-sulfur bacteria, but it is difficult to secure a sufficient quantity of red non-sulfur bacteria necessary for use in preventing large-scale malodor and to put it to practical use. Met.

[0008]

SUMMARY OF THE INVENTION In view of the above-mentioned problems in the prior art, the present invention provides a low-cost, low-cost, red-sulfur non-sulfur bacterium sufficient for use in wastewater treatment equipment. A culture device for red non-sulfur bacteria for efficient supply, and having such a culture device, can perform sanitary and safe treatment of wastewater such as food processing wastewater that emits offensive odor, and capital investment. It is an object of the present invention to provide a low-odor wastewater treatment apparatus capable of effectively suppressing malodor without increasing the number of facilities and increasing the maintenance cost of equipment.

[0009]

According to the present invention, there is provided an apparatus for culturing a red non-sulfuric bacterium according to the present invention for producing a volatile lower fatty acid from a high-concentration organic aqueous solution as described in claim 1 in order to solve the above-mentioned problems. And a culture tank for cultivating a red non-sulfur bacterium using the volatile lower fatty acid generated in the organic acid generation tank as a substrate. Waste liquid supply means for supplying a volatile aqueous liquid, organic acid generation tank odor removal means for removing odor generated in the organic acid generation tank, and aqueous liquid containing volatile lower fatty acids generated in the organic acid generation tank to the culture tank Organic acid supply means, organic acid diluting means for diluting volatile lower fatty acids supplied into the culture tank, oxygen supply means for maintaining the culture tank in an aerobic atmosphere, and a culture tank for removing odor generated in the culture tank Odor removal means and culture In a culture system of purple non-sulfur bacteria and a bacteria supplying means for supplying to the demand side the aqueous solution containing the proliferated purple non-sulfur bacteria.

[0010] In the low odor wastewater treatment apparatus of the present invention, an organic acid generating tank for generating a volatile lower fatty acid from a high-concentration organic aqueous liquid, and the organic acid generating tank is used. A wastewater treatment device having a culture tank for culturing red non-sulfur bacteria using a volatile lower fatty acid as a substrate, wherein wastewater supply means for supplying a high-concentration organic aqueous liquid to the organic acid generation tank, and an organic acid generation tank. Organic acid supply means for supplying an aqueous liquid having the generated volatile lower fatty acid to the culture tank, organic acid dilution means for diluting the volatile lower fatty acid supplied into the culture tank, and red non-sulfur bacteria grown in the culture tank Odor drainage treatment, comprising: a bacteria supply means for supplying an aqueous liquid containing a solution to at least one of a wastewater before aeration treatment, an aeration tank or a sludge storage tank, and an oxygen supply means for maintaining the culture tank in an aerobic atmosphere. Device.

[0011]

BEST MODE FOR CARRYING OUT THE INVENTION In the apparatus for culturing red non-sulfur bacteria according to the present invention, a volatile lower fatty acid is produced by anaerobic fermentation from a high-concentration organic aqueous solution in an organic acid producing tank.

The high-concentration organic aqueous liquid used includes:
Organic solvents such as alcohols, or components that may interfere with anaerobic fermentation performed in an organic acid production tank, such as contained inorganic substances, may be any aqueous liquid that is less than a concentration that does not interfere with fermentation, By using aqueous liquids such as confectionery waste liquid, juice waste liquid, or milk and processed milk that have become unsuitable for food and drinking over time (collectively referred to as “waste milk”), these can be effectively used. Become. In particular, when a red non-sulfur bacterium obtained when waste milk is used as a high-concentration organic aqueous liquid is applied to the treatment of process wastewater in a dairy factory or the like, a high deodorizing effect can be obtained.

The volatile lower fatty acids generated from the high-concentration organic aqueous liquid in the organic acid generating tank are, for example, those having 2 to 6 carbon atoms such as acetic acid, propionic acid, butyric acid, valeric acid and caproic acid.
The volatile lower fatty acid is desirably used. For example, in the case of discarded milk, fermentation is performed so that the concentration of these organic acids is about 20 g / L (about 10 days). Fermentation proceeds without the need for a particular inoculum. In addition,
Heat as necessary and preferably maintain at around 35 ° C.

Since the rate of fermentation in the organic acid production tank is slower than the culture of red non-sulfur bacteria using volatile lower fatty acids as a substrate, a large organic acid production tank is used. It is preferable that only a part of the internal liquid is sent out to the tank, and then a high-concentration organic aqueous liquid corresponding to the amount sent out is recharged so that the internal system is not significantly affected. When the high-concentration organic aqueous liquid is charged again, the pH of the system is adjusted to 6 to 9 by adding an alkaline aqueous solution such as sodium hydroxide as needed.

The aqueous liquid having a volatile lower fatty acid generated in the organic acid generating tank as described above is, for example, a pump and a pipe,
It is sent to the culture tank by an organic acid supply means composed of a valve, a timer, a flow meter, a water level meter and the like. At this time, as described later, in the device of the present invention, due to its unique configuration,
The red non-sulfur bacteria can be cultured in the culture tank without performing a sterilization operation such as heating.

The culture tank must be made of a corrosion-resistant material, for example, stainless steel or FRP, or must be lined with various corrosion-resistant lining materials. In the present invention, the growth of red non-sulfur bacteria is preferably performed in a dark environment because it enables more active growth. Therefore, when the culture tank is installed outdoors, a material that does not transmit light is preferable. Or light-shielded by paint or other means.

The aqueous liquid containing volatile lower fatty acids supplied into the culture tank has a high organic acid concentration of about 20 g / L, and has an organic acid concentration of about 0.5 to 5 g / L, as described later. Is diluted to about 1.5 to 3 g / L and used for culturing, thereby enabling efficient culturing.

For dilution, well water, industrial water, or relatively clear industrial wastewater is added to the aqueous liquid containing volatile lower fatty acids supplied to the culture tank. The organic acid diluting means for this is formed by a stirrer, a pipe, a hose, a pump, a valve, a timer, a flow meter, a water level meter and the like as necessary. Here, an automatic titration device or, if the aqueous liquid containing volatile lower fatty acids is clear, a combination of an absorptiometer, etc., an automatic valve, a CPU, etc., and automatic control of organic acid dilution to obtain an optimal dilution ratio. By providing the means, the organic acid diluting means can be automatically controlled.

In the apparatus of the present invention, since the aqueous solution containing volatile lower fatty acids is diluted and used as described above, the capacity of the organic acid generating tank is several times to about the same as the capacity of the culture tank. Is sufficient, which is advantageous in terms of equipment cost and installation area.

It should be noted that the aqueous liquid containing volatile lower fatty acids has a large amount of germs, which can usually be used for culturing red non-sulfur bacteria without particular sterilization. No. According to the configuration of the device of the present invention, an aqueous liquid containing a red non-sulfur bacterium suitable for deodorization can be obtained without performing sterilization as described above.

The aqueous liquid containing the volatile lower fatty acid is adjusted to an organic acid concentration of 0.5 to 5 g / L, preferably 1.5 to 3 g / L, which is suitable by the organic acid diluting means as described above. When adjusted for cultivation, conventionally 10 days to 3 days
Sufficient growth of red non-sulfur bacteria, which took about a week,
It is possible to greatly shorten the time to 2 to 4 days.

Here, FIG. 1 shows various concentrations of organic acid aqueous solutions (organic acid concentrations of 20%) prepared from aqueous solutions containing volatile lower fatty acids obtained from waste milk by means of organic acid dilution.
g / L (sign ●), 10 g / L (sign □), 2.5 g /
L (sign △) and 0.75 g / L aqueous solution (sign ×)
5 shows the results obtained by examining the time-dependent changes in the number of red non-sulfur bacteria in the culture tank when using E. coli.

FIG. 1 shows that the growth rate of red non-sulfur bacteria is remarkably increased when an aqueous liquid containing a volatile lower fatty acid whose organic acid concentration is adjusted to 2.5 g / L is used.

In general, it is desirable that culture of red non-sulfur bacteria in a culture tank is performed by a batch culture method (fill & draw method). In addition, when the frequency of the aqueous liquid containing the proliferated red non-sulfur bacteria is frequently required, each group is provided with two or more culture tank groups each including one or more independent culture tanks, and the culture in each group is performed. The supply to the demand side from the start to the demand side after the end of the culture can be performed independently by making it possible to perform the supply independently.

In the case of the apparatus of the present invention having the organic acid diluting means as described above, the growth of red non-sulfur bacteria is 2 to 2.
Four days is enough. Here, in order to supply an aqueous liquid containing a sufficient number of red non-sulfur bacteria by batch culture, the growth period of the red non-sulfur bacteria is set to 3 days, and four culture tank groups are set. (One culture tank group is formed from one or more independent culture tanks), and these are independently and sequentially charged with an aqueous liquid containing volatile lower fatty acids, grown for 3 days, grown on the demand side, It can be operated automatically by repeating the feeding cycle and providing a continuous circulating culturing control means that an aqueous liquid containing the grown red non-sulfur bacteria can be obtained from any group every day. In addition,
Such a continuous circulation culture control means includes a relay, a timer,
Various automatic valves, pumps, and the like can be combined.

FIG. 2 shows that the concentration of organic acid obtained from discarded milk was 2%.
0 g / L was diluted to 2,500 mg / L, and the change in the number of bacteria when cultivating red non-sulfur bacteria under various conditions in an aqueous liquid (20 ° C.) containing volatile lower fatty acids was determined. The result of the examination is shown.

In the figure, the symbol ● indicates the result of culturing in a dark place while aerating with air, the square indicates the result of culturing in a bright place with aeration by air, and the symbol △ indicates the result of culturing in a dark place without aeration. The results obtained when the cells were cultured are shown. The vertical axis is wavelength 660 nm
And is proportional to the number of red non-sulfur bacteria.

From these results, it can be seen that when culturing while aerating with air, the culturing is optimal for 2 to 3 days, especially in a dark place. For this reason, it is desirable that the culture tank is made of a material that does not allow light to pass through, or is installed in a dark place.

The red non-sulfur bacterium cultivated in the culturing apparatus of the present invention is classified into Rhodospirillaceae among bacteria belonging to photosynthetic bacteria,
For example, the genera Rhodobacter, Rhodopseudomonas, Rhodospirillum (Rh
odospirillum, Rhodomicrobiu
m), bacteria such as Rhodocyclus can be used for odor removal without any particular limitation. Specific examples of such bacteria include Rhodopseudomonas palstris,
Rhodobacter capsulatu
s), Rhodobacter sphaeroides
phaeroides), Rhodocyclus geratinosas (Rhodocyc)
lus gelatinosus), Rhodospirium labham (Rhodo)
spirillum rubrum).

Such a red non-sulfur bacterium is collected, for example, from soil, lakes and marshes, sewage, sludge, etc. in the natural environment, from excess activated sludge generated from a sewage treatment plant, etc., or is odor-producing. It can be collected from drainage channels and the like, but can also be obtained from Solar Co., Ltd. (trade name: Biocosmo) as a cell product containing photosynthetic bacteria.

For the cultivation of the red non-sulfur bacteria, it is necessary to maintain the culture tank in an aerobic atmosphere. Therefore, the culture tank is provided with an oxygen supply means. The oxygen supply means includes a pipe, a valve, a pump (compressor), a timer, and the like for blowing oxygen or a gas containing oxygen such as air into the culture tank. In addition, since the gas is blown from the bottom of the culture tank, the gas can be stirred at the same time, so that the oxygen concentration of the whole culture solution can be kept high, and more vigorous growth becomes possible.

At the time of culturing, phosphorus, nitrogen and the like can be added in order to further promote red non-sulfur bacteria. At this time, as the phosphorus and nitrogen, those containing compound nitrogen and compound phosphorus are suitable. here,
Examples of the compound containing nitrogen added to the aqueous solution containing volatile lower fatty acid include ammonium chloride, ammonium sulfate, and urea. The content of the volatile lower fatty acid in the aqueous solution is usually 20 to 2 as compound nitrogen. ,
Preferably, it is in the range of 400 mg / L.

Examples of the phosphorus-containing compound added to the aqueous solution containing a volatile lower fatty acid include potassium dihydrogen phosphate and sodium phosphate. Preferably, the phosphorus content is usually in the range of 2 to 600 mg / L.
In addition to such aqueous solutions containing volatile lower fatty acids, in addition to magnesium salts and salt, zinc, copper, iron, cobalt,
Trace salts such as manganese can be added. In addition, the pH of the aqueous solution containing the aqueous volatile lower fatty acid
Is preferably in the range of 6 to 8.

The temperature of the culture tank for the red non-sulfur bacteria is 10 to
Desirably, the temperature is 40 ° C. Further, a part of the red non-sulfur bacteria grown in the culture tank is collected, inoculated into an aqueous solution containing the same volatile lower fatty acid as described above, and the culture can be repeated under the same culture conditions.

In this way, it is obtained by culturing for about 3 days in a culture tank.
Aqueous cultures usually contain one or more red purple non-sulfur bacteria.
General bacteria are also present. Usually, the number of common bacteria is 10^Ten~
10 ¹²About the number of cells per mL, but the number of red non-sulfur bacteria
Is 10⁶Pcs / mL or more, usually 10⁷-10⁸Pcs / mL
It is. In this way, various bacteria are mixed
Even if the number of red non-sulfur bacteria
Can be kept on.

The aqueous liquid containing the red non-sulfurous bacteria grown in the culture tank is supplied to the wastewater treatment apparatus main body, which is the demand side, by the bacteria supply means. The bacteria supply means is piping,
It consists of hoses, pumps, valves, timers, flow meters, water level gauges, etc. In the wastewater treatment device itself, the aqueous liquid containing the multiplied red non-sulfur bacteria is added to the odor-producing aqueous liquid to be deodorized (the waste water before aeration treatment generated in various manufacturing processes), and the number of red non-sulfur bacteria is increased. Is added so as to be 10 ⁴ cells / mL or more. At this time, the aerobic treatment is performed at a temperature of 10 to 40 ° C.

In the main body of the low-odor wastewater treatment apparatus, the content of lower fatty acids, particularly volatile lower fatty acids, contained in the odor-producing aqueous liquid is reduced, so that generation of odors based on such lower fatty acids is effectively suppressed. be able to. The treated water from which the odor has been removed by the present invention is usually 10 ⁵ to
Although it contains about 10 ⁸ cells / mL of red non-sulfur bacteria, it can be directly supplied to a biological treatment apparatus such as an ordinary activated sludge method to remove pollutants such as BOD. The treated wastewater can be safely drained in the same manner as ordinary wastewater after biological treatment.

The low-odor wastewater treatment apparatus according to the present invention is capable of treating a culture solution containing the red non-sulfur bacteria with a bacterial count of at least 10 ⁶ / mL, preferably at least 10 ⁷ / mL, with respect to the odor-producing wastewater. Before or during aeration,
Alternatively, the sludge to be transferred to the sludge storage tank from the settling tank, preferably a purple non-sulfur bacteria the number of the bacteria 10 ⁴ cells / mL
It is added so as to be as described above.

In the apparatus for culturing red non-sulfur bacteria according to the present invention, the organic acid producing tank and the culturing tank generate an odor, which is also called an offensive odor, during the fermentation. In particular, when the high-concentration organic aqueous liquid is a food liquid such as waste milk, it often has a bad smell that cannot be directly discharged. Therefore, an organic acid generation tank odor removing means and a culture tank odor removing means for removing the odor are provided.

These odor removing means include, for example, a deodorizing device such as an exhaust fan, an activated carbon deodorizing device, a biological deodorizing device, or an aeration tank of an activated sludge device, and a piping for sucking odors generated in an organic acid generating tank and a culturing tank, respectively. , A hose or the like. Here, the odor generated in the organic acid generation tank and / or the culture tank is introduced into the aeration tank of the activated sludge apparatus to which the red non-sulfur bacteria, which can be cultured in a large amount by the apparatus of the present invention, is added. By doing so, it is possible to effectively deodorize.

In the culturing apparatus of the present invention, there is provided a bacterium counting means for counting the number of red non-sulfur bacteria in the above-mentioned culturing tank, and when the number of red non-sulfur bacteria in the culturing tank reaches a predetermined number. And a culture tank control means for controlling the bacterial supply means to supply the aqueous liquid containing the red non-sulfur bacteria in the culture tank to the demand side, so that even if a difference occurs in temperature or other culture conditions, An aqueous liquid of a certain number of red non-sulfur bacteria can be supplied to the demand side. In addition, as a bacteria count measuring means for counting the number of red non-sulfur bacteria in the culture tank, a turbidity sensor, a fluorescence sensor, and the like can be mentioned.

Further, the culture tank control means controls the bacterial supply means to supply the red non-sulfur bacteria in the culture tank to the demand side, and then controls the organic acid supply means to culture the volatile lower fatty acids. When supplied to the tank, the culture apparatus for the red non-sulfur bacteria can be operated virtually unattended. As such a culture tank control means, valves, pumps and pipes, hoses, timers, which are linked with the output of the bacteria count measuring means,
It can be configured using a relay or the like.

The aqueous liquid containing red non-sulfur bacteria grown in the culture tank as described above is supplied to at least one, and preferably all, of the wastewater before aeration treatment in the wastewater treatment equipment, the aeration tank or the sludge storage tank. In addition, the odor generated in the wastewater treatment can be significantly reduced.

Here, FIG. 3 shows a flowchart of one example of the low-odor wastewater treatment apparatus according to the present invention. The reference numeral A in FIG. 3 shows the device for culturing red non-sulfur bacteria according to the present invention. The culture device A for red non-sulfur bacteria has a high-concentration organic Waste liquid supply means α for supplying an aqueous liquid, organic acid generation tank β for generating volatile lower fatty acids from high-concentration organic aqueous liquid, organic acid generation tank odor removal means γ for removing odor generated in organic acid generation tank β An organic acid supply means δ for supplying an aqueous liquid having a volatile lower fatty acid generated in the organic acid generation tank β to the culture tank ε, and an organic acid dilution means for diluting the volatile lower fatty acid supplied into the culture tank ε. A culture tank ε for culturing red non-sulfur bacteria using the volatile lower fatty acid generated in the organic acid generation tank β as a substrate, an oxygen supply means η for maintaining the culture tank in an aerobic atmosphere, and an odor generated in the culture tank ε. Cultivation tank odor removing means θ to be removed, and proliferated in the culture tank ε An aqueous liquid containing a red non-sulfur bacterium that has bacterial supply means ι for supplying an aqueous liquid containing a color non-sulfur bacterium to the demand side (the main body of the low odor wastewater treatment apparatus of the present invention) Is supplied to three places: a wastewater before aeration treatment (in this example, a wastewater before aeration treatment in a raw water storage tank), an aeration tank and a sludge storage tank.

Hereinafter, the apparatus for culturing red non-sulfur bacteria of the present invention will be specifically described with reference to the drawings, but the present invention is not limited thereto. FIG. 4 is a piping diagram of the apparatus for culturing red non-sulfur bacteria according to the present invention.

In the figure, reference numeral 1 denotes an organic acid generating tank for generating volatile lower fatty acids from a high-concentration organic aqueous liquid, and a motor for stirring the liquid charged in the tank. Stirring device 1 composed of shaft and stirring blade
The exhaust pipe 1b and the exhaust fan 1c are attached as an organic acid generation tank odor removing means for removing the odor generated in the organic acid generation tank.

The high-concentration organic aqueous liquid charged into the organic acid generating tank 1 is waste milk in this case, and is discharged from the waste milk tank 2 by the pump 2a, which is a waste liquid supply means, and the piping attached thereto. Milk is introduced into the organic acid generator.

In order to maintain the liquid temperature of the organic acid generation tank 1, as a means for heating the organic acid generation tank, the aqueous liquid inside is supplied to the heat exchanger 1 e by a pump 1 d from a pipe provided near the bottom of the organic acid generation tank 1. After that, it circulates to the upper part of the organic acid generating tank 1. Heat exchanger 1e is a hot water boiler (not shown)
Is supplied by the pump 1f. The temperature in the organic acid generation tank is maintained at about 35 ° C. by a temperature control valve 1 g.

The aqueous solution having a volatile lower fatty acids obtained by organic acids within production tank 1, piping 3a, a pump 3b, the valve 3c, pipe 3d, the pipe 3e ₁ ~3e ₄ and the valve 3f ₁ ~
Composed of 3f _4, the organic acid supply means for supplying the culture tank with an aqueous solution having a volatile lower fatty acids produced by the organic acid production tank, fermenter ₄₁ to ₄ from near the bottom of the organic acid production tank 1 Introduced in either.

After the aqueous liquid having a volatile lower fatty acid generated in the organic acid generating tank is supplied by the organic acid supplying means for supplying the culture tank, the high-concentration organic liquid is again supplied to the organic acid generating tank whose liquid level has decreased. The aqueous liquor is added by the waste liquid supply means. At this time, if necessary, the aqueous solution in the organic acid producing tank is adjusted to have a pH in the range of 6 to 9 suitable for fermentation.
An aqueous sodium hydroxide solution is added by the pH adjusting means of the organic acid generating tank consisting of j.

In this embodiment, a pump 5b and a valve 5c for feeding dilution water to each culture tank from a factory drain pit 5a in which this apparatus is installed and diluting the volatile lower fatty acids supplied into the culture tank are provided. through, the pipe 3d, piping 3e ₁ ~3e ₄
And organic acids diluting unit is provided constituted by a valve 3f ₁ ~3f _4.

A culture tank for cultivating red non-sulfur bacteria using the volatile lower fatty acid generated in the organic acid generation tank as a substrate, and the volatile lower fatty acid supplied to the culture tank is diluted,
A red non-sulfur bacterium is introduced into a culture tank, and cultivation is performed using volatile lower fatty acids as a substrate.

It is not necessary to introduce the red non-sulfur bacterium every time. When the aqueous liquid containing the red non-sulfur bacterium grown by culturing in the culture tank is supplied to the demand side as described later, Leave the aqueous liquid in the culture tank about 1/10,
By adding an aqueous liquid having a volatile lower fatty acid from the organic acid generation tank and dilution water thereto, cultivation can be performed again.

In the case of the apparatus of the present invention, about three days are sufficient for cultivation (proliferation) of red non-sulfur bacteria, for example, four independent culture tank groups are provided, and any one of By sequentially charging the culture tank group, an aqueous liquid containing a red non-sulfur bacterium that has sufficiently grown can be supplied to the demand side every day. In this example, four groups are provided each of the culture tank ₄₁ to ₄ as one group.

[0055] Each the oxygen supply means to each of the culture tank ₄₁ to ₄ to keep the culture vessel aerobic atmosphere (composed of a pump for supplying air to the air pipe 5b ₁ ~5b ₄₎ Air is blown from the bottom of the culture tank. In addition, by this air blowing, the aqueous liquid in the culture tank is agitated, and uniform and effective multiplication can be achieved.

[0056] culture tank ₄₁ to ₄ purple non-sulfur bacteria odors generated by cultures from each culture tank upper pipe 6a ₁ ~6a ₄ in the culture tank odor removal constituted by a pipe 1b and the fan 1c Aspirated by means. The exhaust pipe 1
b and the exhaust fan 1c are used also as an odor removing means for the organic acid generating tank. The gas containing odor discharged from the exhaust fan 1c is introduced into an aeration tank of an activated sludge device (not shown) to be deodorized.

[0057] Aqueous solution containing the proliferated purple non-sulfur bacteria in a culture tank, pipe 7a ₁ to 7-the valve respectively included
a _4, it is supplied to the demand side by configured bacteria supplying means through a pipe 7b, and a pump 7c ₁ ~7c _3. That is, by a pump 7c ₁ ~7c ₃ In this example, products of milk as a raw material (milk, butter, whey, etc.) the process of production, an aqueous solution containing purple non-sulfur bacteria are added to the industrial wastewater generated by device washing process Thus, odor generation during activated sludge treatment of these factory effluents is suppressed.

Here, an aqueous liquid containing red non-sulfur bacteria (red red non-sulfur bacterium) containing waste non-sulfur bacteria grown using the red non-sulfur bacterium culturing apparatus of the present invention using waste milk as the high-concentration organic aqueous liquid Of bacteria (2 × 10 ⁹ cells / mL) from a dairy factory (odor-producing aqueous liquid, drainage 1,000 m)
³ / day) raw water storage tank of wastewater treatment equipment (capacity: 1,000
m ³ ), aeration tank (capacity: 1,000 m ³ ), activated sludge storage tank (capacity: 30 m ³ (the activated sludge withdrawal amount is 30 t)
/ Day)), the number of red non-sulfur bacteria is 5
The change in the odor before and after the addition at each location when added daily so as to be × 10 ⁶ pieces / mL was examined. Table 1 shows the definition of the odor degree, and Table 2 shows the evaluation results.

[0059]

[Table 1] 定義 Odor level definition ━━━━━━━━━━━━━ １ 1 Almost no smell. 2 Slight smell 3 Smell is felt even though it is weak 4 Smell that clearly feels bad smell 5 Strong smell 6 Strong smell ━━━━━━━━━━━━━ ━━━━━━━━━━━━━━━━━

[0060]

[Table 2] ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Odor level Before addition After addition (7 days after start of addition) ━━ ━━━━━━━━━━━━━━━━━━━━━━━━━━━━ Raw water storage tank 3.5 1 ──────────────── ────────────── Aeration tank 1 1 ────────────────────────────── Sludge storage tank 5 1 ━━━━━━━━━━━━━━━━━━━━━━━━━━━━━━

According to Table 2, when the aqueous liquid containing the red non-sulfur bacteria grown by the red non-sulfur bacteria cultivation apparatus according to the present invention was added to the wastewater treatment apparatus, the effect of reducing the generation of offensive odor was remarkably large. Understand

[0062]

Industrial Applicability The low-odor effluent treatment apparatus of the present invention can perform sanitary and safe treatment of wastewater such as food processing effluent that produces an odor, and can reduce the capital investment and increase the maintenance cost of the equipment. Without this, the odor can be effectively suppressed. Further, the culture device for red non-sulfur bacteria of the present invention can efficiently culture red non-sulfur bacteria.

[Brief description of the drawings]

FIG. 1. Bacteria of red non-sulfur bacteria in a culture tank when organic acid aqueous solutions of various concentrations prepared by an organic acid diluting means from an aqueous solution containing volatile lower fatty acids obtained from waste milk are used. It is a figure showing the result of having examined change with time of the number.

FIG. 2 is a diagram showing a change in the number of red non-sulfur bacteria due to a difference in culture conditions.

FIG. 3 is a flowchart of an example of a low-odor wastewater treatment apparatus of the present invention.

FIG. 4 is a piping diagram of an apparatus for culturing red non-sulfur bacteria according to the present invention.

[Explanation of symbols]

 A Culture device for red non-sulfur bacteria α Waste liquid supply means β Organic acid generation tank γ Organic acid generation tank odor removal means δ Organic acid supply means ε Culture tank ζ Organic acid dilution means η Oxygen supply means θ Culture tank odor removal means ι Bacteria Supply means

[Procedure amendment]

[Submission date] September 7, 2000 (2009.7)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Claims

[Correction method] Change

[Correction contents]

[Claims]

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0009

[Correction method] Change

[Correction contents]

[0009]

According to the present invention, there is provided an apparatus for culturing a red non-sulfuric bacterium according to the present invention for producing a volatile lower fatty acid from a high-concentration organic aqueous solution as described in claim 1 in order to solve the above-mentioned problems. And a culture tank for cultivating a red non-sulfur bacterium using the volatile lower fatty acid generated in the organic acid generation tank as a substrate. Waste liquid supply means for supplying a volatile aqueous liquid, organic acid generation tank odor removal means for removing odor generated in the organic acid generation tank, and aqueous liquid containing volatile lower fatty acids generated in the organic acid generation tank to the culture tank Organic acid supply means, organic acid diluting means for diluting volatile lower fatty acids supplied into the culture tank, oxygen supply means for maintaining the culture tank in an aerobic atmosphere, and a culture tank for removing odor generated in the culture tank Odor removal means and culture In an aqueous solution containing the proliferated purple non-sulfur bacteria and a bacteria supplying means for supplying to the demand side, each group
Consists of one or more independent batch culture tanks
More than one culture tank group, and start culture in each culture tank group
From the end of the culture to the supply to the demand side
In addition, these culture tank groups are sequentially and independently volatile lower fatty acids.
Of aqueous liquid containing, growth culture for 2-4 days, demand side
Circulating culture control means to repeat the supply cycle
It is a cultivation device for red non-sulfur bacteria that has.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0010

[Correction method] Change

[Correction contents]

[0010] In the low odor wastewater treatment apparatus of the present invention, as described in claim 5, an organic acid generating tank for generating a volatile lower fatty acid from a high-concentration organic aqueous liquid, and the organic acid generating tank is used. A wastewater treatment device having a culture tank for culturing red non-sulfur bacteria using a volatile lower fatty acid as a substrate, a waste liquid supply means for supplying a high-concentration organic aqueous solution to an organic acid generation tank , and the organic acid generation tank Yes to remove odors generated by
Mechanical acid generating tank odor removing means, organic acid supplying means for supplying an aqueous liquid having a volatile lower fatty acid generated in the organic acid generating tank to the culture tank, organic acid for diluting the volatile lower fatty acid supplied into the culture tank Dilution means, maintain the culture tank in aerobic atmosphere
Oxygen supply means to remove odors generated in the culture tank
The cultivation tank is preferably provided with a tank odor removing means and a bacteria supply means for supplying an aqueous liquid containing red non-sulfur bacteria grown in the culture tank to at least one of drainage before aeration treatment, an aeration tank or a sludge storage tank. Oxygen supply means for maintaining an aerobic atmosphere , wherein each group comprises one or more independent batch culture type culture tanks.
Provided with at least two culture tank groups
The process from start of culture to supply to the demand side after culture is
The culture tank groups are independently and sequentially volatile
Of aqueous liquid containing secondary fatty acids, growth for 2-4 days
Continuous circulation culture that repeats the cycle of feeding and supply to the demand side
This is a low odor wastewater treatment device having a control means .

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) C12N 1/20 C12N 1/20 A // (C12N 1/20 (C12N 1/20 A C12R 1:01) (C12R 1:01) (72) Inventor Yoshiaki Shibuya 4-4 Midorigahara, Tsukuba-shi, Ibaraki Prefecture Aquas Co., Ltd. Within Tsukuba Research Institute (72) Inventor Masao Ikeya 4-4 Midorigahara, Tsukuba-shi, Ibaraki Aquas Co., Ltd. (72) Inventor Hiroaki Yabuzaki 1-3-43 Kawaramachi, Wakabayashi-ku, Sendai, Miyagi Prefecture F-term (reference) 4A029 AA02 BB02 CC01 DA10 DB11 DF03 DF04 DF05 DF06 DF10 DG10 4B065 AA01X AC09 AC20 BB08 BC06 BC11 BC14 BD50 CA55 CA56 4D040 DD04 DD24

Claims (7)

[Claims] 1. An organic acid producing tank for producing a volatile lower fatty acid from a high-concentration organic aqueous liquid, and a culture vessel for culturing a red non-sulfur bacterium using the volatile lower fatty acid produced in the organic acid producing tank as a substrate. And a waste liquid supply means for supplying a high-concentration organic aqueous liquid to the organic acid generation tank,
Organic acid generating tank odor removing means for removing the odor generated in the organic acid generating tank, organic acid supplying means for supplying an aqueous liquid having a volatile lower fatty acid generated in the organic acid generating tank to the culture tank,
Organic acid diluting means for diluting volatile lower fatty acids supplied into the culture tank, oxygen supply means for maintaining the culture tank in an aerobic atmosphere, culture tank odor removing means for removing odor generated in the culture tank, and A cultivation apparatus for a red non-sulfur bacterium, comprising: a bacterium supply means for supplying an aqueous liquid containing a red non-sulfur bacterium grown in a culture tank to a demand side. 2. The apparatus for cultivating a red non-sulfuric bacterium according to claim 1, wherein the culture tank is a batch culture type culture tank. 3. The culture tank control means controls the bacterial supply means to supply the aqueous liquid containing the red non-sulfur bacteria in the culture tank to the demand side, and then controls the organic acid supply means to volatilize. 3. The apparatus for cultivating a red non-sulfuric bacterium according to claim 1 or 2, wherein the functional lower fatty acid is supplied into a culture tank. 4. Each group is provided with two or more culture tank groups each composed of one or more independent culture tanks, and the supply to the demand side after the start of culture in each group and after the end of culture is independently performed. 4. The cultivation apparatus of a red non-sulfur bacterium according to any one of claims 1 to 3, wherein the apparatus can be performed. 5. A culture tank group comprising four culture tank groups, each of which is independently and sequentially charged with an aqueous liquid containing a volatile lower fatty acid, grown for three days, and supplied to the demand side. The red non-sulfuric acid according to claim 4, further comprising a continuous circulating culture control means for repeating the cycle and obtaining an aqueous liquid containing the red non-sulfurous bacteria grown from any group every day. Bacterial culture equipment. 6. The apparatus for culturing a red non-sulfur bacterium according to claim 1, wherein the high-concentration organic aqueous liquid is waste milk. 7. An organic acid producing tank for producing a volatile lower fatty acid from a high-concentration organic aqueous liquid, a culture vessel for culturing a red non-sulfur bacterium using the volatile lower fatty acid produced in the organic acid producing tank as a substrate. Wastewater supply means for supplying a high-concentration organic aqueous liquid to an organic acid generation tank, and an organic acid for supplying an aqueous liquid having a volatile lower fatty acid generated in the organic acid generation tank to a culture tank Supply means, organic acid diluting means for diluting volatile lower fatty acids supplied to the culture tank, at least one of an aqueous solution containing a red non-sulfurous bacterium grown in the culture tank, a wastewater before aeration treatment, an aeration tank or a sludge storage tank. A low-odor wastewater treatment apparatus, comprising: a bacteria supply means for supplying a bacterium; and an oxygen supply means for maintaining the culture tank in an aerobic atmosphere.