JP2001095910A - Deodorant composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a deodorant composition which is reduced in discoloration of a formulation before use and adverse effects on the use environment and can efficiently deodorize offensive odor. SOLUTION: This deodorant composition contains a laccase being an oxidase, and a phenolic compound glycoside. Rutin and derivatives thereof, gallic acid glycoside and cyanine are preferably used as the phenolic compound glycoside.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a deodorant composition which has an excellent effect of removing malodors generated at home, and has solved the problems of formulation stability until use and coloring in the use environment. is there.

[0002]

BACKGROUND OF THE INVENTION Conventionally, a plant extract having a deodorizing power as an effective deodorizing component has been used as a deodorant for removing bad breath and for removing malodors generated in general households and factories. A deodorant blended with
No. 4278, No. 61-240960), a deodorant using chemicals such as sodium perborate and hydrogen peroxide, and a deodorant focusing on the deodorizing power of quinone (Japanese Patent Publication No. Sho.
Nos. 1-39774 and 53-45372) are known.

[0003] However, these deodorants have weak deodorizing power and have insufficient effects. Further, the deodorant using a chemical agent has a relatively strong deodorizing effect, but has a problem in terms of safety to the human body. Therefore, attempts have been made to develop a deodorant having excellent properties such as strong deodorizing power and safe use.

As one of the attempts, a deodorant using an effective deodorant component and an oxidoreductase has been proposed. As such an example, a combination of a plant extract and an oxidoreductase (Japanese Patent Publication No. 7-53174, Japanese Unexamined Patent Publication No.
Japanese Patent Application Laid-Open No. Hei 9-38183) and those using a combination of a phenolic compound and an oxidase (JP-A-9-38183) are known. Among these oxidoreductases, laccase requires oxygen for expression of its activity, and is therefore particularly effective because it can be expected that it can be stored in a single-drug form.

[0005] However, although the deodorant obtained by combining the deodorant active ingredient and the enzyme is effectively excellent, the polymerization reaction of the deodorant active ingredient proceeds by the oxidase when exerting the effect, and the There was a problem at the time of deodorizing treatment at home, such as soiling the clothes of the user. In addition, deodorant active ingredients such as catechol may cause coloring during the storage period after preparation of the preparation, and there is also a problem in practical aspects.

Accordingly, the deodorant composition of the present invention can solve the problems associated with coloring of the deodorant active ingredient, and can effectively deodorize odors generated in the home by combining it with laccase. It is intended to provide a composition for use.

[0007]

Means for Solving the Problems and Embodiments of the Invention
The present inventor has conducted intensive studies in search of beneficial deodorant active ingredients. As a result, the use of glycosides of phenolic compounds alleviates the above-mentioned coloring problem, and deodorizes by combining laccase. It has been found that the action is high, and the present invention has been accomplished.

[0008] That is, the present invention provides a deodorizing composition characterized by comprising a phenolic compound glycoside and laccase, one of oxidases, as active ingredients for deodorizing. Hereinafter, the present invention will be described in detail.The deodorant composition of the present invention is obtained by blending an effective deodorant ingredient and laccase. Smell of odors generated in the human body, house, environment, etc., such as odor, tobacco smell in the house, odor of sewage and feces in toilet, odor of garbage and decay, odor of pet, and odor of car exhaust gas and odor in car. Widely used as odorant. Particularly effective as a deodorant for garbage odor and bad breath.

[0009] The deodorant composition of the present invention uses laccase as an oxidoreductase. Here, the laccase used in the present invention is E. coli. C. It is an enzyme classified into 1.10.3.2. As a typical reaction example, it is known that urushiol is oxidized by laccase in lacquer sap to form lacquer. Laccase is an enzyme that widely exists in many plants and microorganisms besides lacquer and catalyzes the oxidation reaction of aromatic compounds, and can be used in the present invention regardless of its origin.

The amount of the laccase to be used in the present invention in the composition includes the form of the composition, the frequency of use,
Although it depends on the treatment time and the titer of the enzyme preparation, it is usually 0.000.
5% by weight (hereinafter abbreviated as%) or more, preferably 0.00%
It is better to mix 5% or more. If it is less than 0.0005%, a sufficient effect cannot be obtained. In addition, even if it exceeds 1%, the effect corresponding to the increase in the added amount is not increased,
It is preferably at most 1%, particularly preferably at most 0.5%.

Next, a deodorant substrate is added to the deodorant composition of the present invention. The deodorant substrate used in the deodorant composition of the present invention is characterized by using a glycoside of a phenolic compound.
The glycoside of the phenolic compound can be used without particular limitation as long as it is a glycoside of a compound in which the hydroxyl group in the structure is oxidized by the above enzyme.

Specific examples of such compounds include flavonoid glycosides such as swertiajaponin, robinin, vitexicarpine and flavonol and flavanone glycosides such as quercitrin, myricitrine, hesperidin, rutin and derivatives thereof. Is mentioned. As a derivative of rutin, a compound in which one or more glucoses are further bound to glucose bound to the flavonoid moiety can be mentioned as a derivative. These compounds are industrially available (G-Rutin, manufactured by Toyo Seika Co., Ltd.). In this case, the water solubility is particularly high as compared with other glycosides, which is preferable. Further, as a glycoside of a phenolic compound other than flavonoids, hydroquinone glucoside,
Gallic acid glucoside and cyanine;

More preferably, rutin and its derivatives,
For example, a compound in which one or more glucoses are further bound to glucose bound to the flavonoid portion, gallic acid glucoside, cyanine and the like can be mentioned.

The amount of the deodorant active ingredient of the present invention varies depending on the type of the deodorant, etc., but is 0.0005 to 5%, particularly 0.001 to 5% in terms of solid content based on the total amount of the deodorant. 1
% Is preferable. If the content is less than 0.001%, a sufficient effect cannot be obtained, and even if the content exceeds 1%, the effect corresponding to the increase in the added amount does not increase. The glycosides of the phenolic compounds may be used alone or as a mixture of two or more.

Further, the composition of the present invention may be formulated into a foamed preparation by incorporating a surfactant. Laccase takes in oxygen in the air and the reaction proceeds. Therefore,
In the case of the foamed form, oxygen is efficiently taken in, and high reactivity of the deodorant composition of the present invention is expected.

The surfactant used herein is preferably a nonionic surfactant, in particular, a polyoxyethylene alkyl ether, a fatty acid alkylolamide, or an acylglutamic acid surfactant, used alone or in combination of two or more.

More specifically, examples of polyoxyethylene alkyl ether include polyoxyethylene stearyl and polyoxyethylene hydrogenated castor oil, and examples of fatty acid alkylolamide include coconut oil fatty acid diethanolamide. Examples of acylglutamic acid type surfactants include C12-C18 saturated and unsaturated fatty acids, as well as coconut oil fatty acids, hydrogenated coconut oil fatty acids, palm oil fatty acids, hydrogenated palm oil fatty acids, tallow fatty acids, hydrogenated tallow fatty acids which are mixtures thereof. Glutamic acid esters such as Specific examples include N-coconut fatty acid acyl-L-glutamate triethanolamine, lauroyl-L-glutamate triethanolamine, N-coconut fatty acid acyl-L-sodium glutamate, N-lauroyl-L-sodium glutamate, Examples include sodium N-myristoyl-L-glutamate, N-coconut fatty acid / hardened tallow fatty acid acyl-sodium L-glutamate, and N-coconut fatty acid acyl-potassium L-glutamate.

Among them, acyl glutamic acid type surfactants are particularly preferred. When these are used, the enzyme activity of laccase hardly decreases at a concentration generally used. Specifically, N-coconut fatty acid acyl-L-glutamate triethanolamine,
N-coconut oil fatty acid acyl-L-glutamate sodium, lauroyl-L-glutamate triethanolamine, and N-lauroyl-L-glutamate sodium.

The concentration of the surfactant used in the present invention in the composition is usually 0.05 to 5% by weight, preferably 0.1 to 2% by weight.
If it is less than 0.05% by weight, sufficient foaming power cannot be obtained,
If it exceeds 5% by weight, the enzyme activity of laccase may be affected.

When a surfactant is used as described above, it is preferable that the surfactant is used in a liquid state at the time of preparation and foamed at the time of use to use it in a foamed state. In this case, as a means for forming the composition into a foam, a known method is employed. For example, a method in which the composition is passed through a mesh in a container to form a foam-form preparation can be mentioned.

Further, in the deodorizing composition of the present invention, the oxygen concentration in the composition is reduced to about 0.00015% by weight, and a container having low oxygen permeability is employed to suppress the increase in the oxygen concentration during the storage period. You can do it. In this case, the activity of laccase can be maintained, which is extremely preferable. A known method is employed as a method for preparing such a composition. The method for lowering the oxygen concentration can be achieved by a method for lowering the dissolved oxygen concentration by degassing or by passing a nitrogen gas or an argon gas. As a container having a low oxygen permeability, the material constituting such a container has an oxygen permeability of 10 × 10 ¹⁰ cc · mm / sec · cm ² ···
It is less than cmHg, and specific examples thereof include glass, aluminum, acetal resin, polyethylene terephthalate resin, and vinyl chloride resin. Further, a method in which a foaming preparation is prepared by filling a pressure-resistant container together with a usual propellant, carbon dioxide, LPG, or the like can be employed.

In the composition of the present invention, in addition to the above-mentioned components, raw materials such as oils, lower alcohols, antioxidants, chelating agents, pH adjusters, preservatives, fragrances and the like can be blended.

[0023]

According to the present invention, there is provided a deodorizing composition capable of reducing the coloring property of the preparation until use and the adverse effect on the use environment and effectively eliminating malodors. .

[0024]

EXAMPLES Hereinafter, the present invention will be described in detail with reference to experimental examples, examples, and comparative examples, but the present invention is not limited to the following examples.

1. Evaluation of Deodorizing Function (Examples 1 to 5, Comparative Examples 1 to 6) In order to clarify the deodorizing effect of the product of the present invention, evaluation was performed using mercaptan, which is a typical malodorous substance. That is,
1. Include 1 ml of 100 ppm ethyl mercaptan solution.
The composition shown in Table 1 was added to the 8-liter mayonnaise bottle from the entrance of the silicon stopper previously attached to the upper part, and the reaction was started. The reaction was carried out at room temperature for 20 minutes, and the amount of ethyl mercaptan in the head space after a predetermined time was measured by gas chromatography. In addition, the deodorization rate (%) was calculated by the following equation. The results are shown in Table 1. Deodorization rate (%) = [1- (amount of ethyl mercaptan 20 minutes after reaction / amount of ethyl mercaptan immediately after start of reaction)] × 1
00

[0026]

[Table 1]

From Table 1, it is clear that the composition of the present invention exhibits a high deodorizing effect when combined with laccase.

2. Formulation stability, dyeing property to use environment (Examples 6-8, Comparative examples 7-8) In order to clarify the stability of the product of the present invention in use conditions, use after preparing a deodorant composition The colorability up to the time and the colorability after the deodorization reaction were examined. Furthermore, in order to examine the dyeing property to the use environment, the coloring property on the plastic surface was examined. That is, the products of the present invention and the compositions of Comparative Examples shown in Table 2 were prepared, and the degree of coloring after standing at room temperature for 3 days was visually determined according to the following criteria. To this, laccase was added so as to be 0.005% by weight, and the composition was pseudo-deodorized and converted into a composition. At this time, the coloring property was visually determined according to the following criteria, and the coloring property after the reaction was determined. 0.5 ml of the composition after the reaction was dropped into a plastic container. After leaving to dry at room temperature, it was rubbed off with a sponge under running water. The dyeing property on the plastic was visually determined according to the criteria for dyeing property. The results are shown in Table 2.

<Criterion for judging colorability> 色: No change in color ○: Little change in color is observed △: Discoloration slightly to brown ×: Discoloration to black <Criteria for dyeing property> ：: No residual pigment was observed. ×: Discolored with residual pigment remaining.

[0030]

[Table 2]

As is evident from Table 2, the product of the present invention has a higher coloring property during storage, a higher coloring property after the reaction, and a better dyeing to the use environment than the conventionally known deodorant substrate catechol. It turned out to be excellent without any problems in terms of sex. Comparative Example 8 is the result of using the aglycone in which the sugar moiety of the phenolic compound glycoside used in the present invention was removed. From the comparison with this, the glycoside became the glycoside, and the coloring property after the reaction was further improved. It was found that there was no problem in terms of the dyeing property to the use environment, and it was excellent.

3. Deodorant performance comprehensive evaluation (Examples 9 to 10,
Comparative Example 9) In order to clarify the usefulness of the product of the present invention in an actual deodorizing environment, a deodorant composition of the product of the present invention shown in Table 3 and a composition of a comparative example were prepared and evaluated by 10 expert panelists. Was done. 40 g of cabbage and 20 g of saury were placed in a commercially available triangular corner, placed in a 40 liter desiccator equipped with a silicon stopper on the top, and kept at 35 ° C. for 2 days to putrefaction. The deodorant composition of the present invention or the comparative example was dropped from a silicon stopper on the upper part of the desiccator, and a deodorizing reaction was performed. One hour after the reaction, an odor sample was subjected to a sensory evaluation (odor intensity and pleasant / unpleasant degree), and this was used as an index of the deodorizing effect and judged based on the following criteria. After the determination of the deodorizing effect, the triangular corners were taken out, and the dyeing properties were visually determined according to the criteria for dyeing properties described above. The results are shown in Table 3.

<Criteria for Deodorizing Effect> (i) Odor intensity (ii) Pleasant / unpleasant score Odor intensity Score Pleasant / unpleasant degree 0 No odor 0 Neither pleasant nor unpleasant 1 Smell barely perceptible -1 Slightly unpleasant 2 Weak smell to know what smell -2 Discomfort 3 Smell that can be easily perceived -3 Very uncomfortable 4 Strong smell -4 Extremely uncomfortable 5 Strong smell

[0034]

[Table 3]

From Table 3, it is apparent that the product of the present invention exerts an effective deodorizing effect on the actual garbage odor. Catechol, a deodorant substrate that has been rejected in the past, was also effective in terms of deodorizing effect. It was found that the corners were discolored, indicating an adverse effect on the use environment. In this respect, the product of the present invention was found to be excellent without any problem.

[0036]

The above composition excluding laccase was prepared, and a pressure-resistant container coated with aluminum (oxygen permeability 5.0 ×
10 ¹⁰ cc. mm / sec. cm ² . less than cmHg)
And the oxygen concentration in the composition is reduced to 0.0
0015% by weight or less. Further, after laccase was added at the concentration shown above, it was quickly sealed with an aluminum cap. An aerosol formulation was prepared by replacing the internal gas with liquefied petroleum gas.

[0038]

The above composition excluding laccase was prepared, and the oxygen concentration in the composition was reduced to 0.0001 by passing argon gas.
% By weight or less. Further, laccase was added to the above concentration to impregnate the nonwoven fabric (100% cotton nonwoven fabric: basis weight 100 g / m ² , impregnation amount: 1 part by weight of sheet and 3 parts by weight of impregnating liquid). This was put in an aluminum zipper bag, and the inside gas was replaced with argon gas and sealed.

(Formulation Example 3) Powdery formulation 1.0 g of citric acid, 1.8 g of disodium hydrogen phosphate,
Rutin derivative (having a sugar chain of glucose instead of rhamnose, trade name G-rutin, manufactured by Toyo Seishi Co., Ltd.) 4
0 mg was added to 1 mg of laccase, pulverized, mixed and placed in an aluminum sample bag (oxygen permeability 5.0 × 10 ^10).
cc · mm / sec · cm ² · cmHg). Sealing was performed in a state where the oxygen concentration was reduced to 0.00015% by weight or less by passing through nitrogen gas to obtain a powdery preparation.

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Claims (2)

[Claims] 1. A deodorant composition comprising an oxidase laccase and a glycoside of a phenolic compound. 2. The deodorizing composition according to claim 1, wherein the glycoside of the phenolic compound is at least one selected from rutin and derivatives thereof, glucoside gallate, and cyanine.