JP2000287626A - Feed assistant for pig farming - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a feed assistant for pig farming capable of promoting the health of livestock, especially pig, lowering the pH of feces to increase the amount of organic acid in feces and improving the feed efficiency without using antibiotic substance by using bacterial cell of Lactobacillus casei as an active component. SOLUTION: Bacterial cell of Lactobacillus casei such as Lactobacillus casei YIT-9029 (FERM BP-1366) is used as an active component. Preferably, the bacterial cell of Lactobacillus casei capable of fattening the intestinal cecum of pig is used as an active component and a pig is administered with the cell at a rate of 1×1010 cfu/day/head for >=2 weeks to fatten the inner volume of the intestinal cecum to >=3 times. In the case of compounding the assistant to feed, the daily administration rate of the cell of Lactobacillus casei is preferably 1×1010 to 1×1011 cfu per head.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a Lactobacillus
The present invention relates to an auxiliary agent for swine feed containing a casei cell as an active ingredient.

[0002]

2. Description of the Related Art Conventionally, so-called compound feed has been generally used as a feed used for pig raising. This compound feed promotes breeding of pigs and has various advantages such as prevention of disease and transmission of swine.

[0003] However, such a compound feed contains an antibiotic, and it is difficult to maintain the growth of young animals without the use of an antibiotic. However, the downside of using antibiotics is also becoming a problem. That is, abuse of antibiotics causes resistant bacteria to appear, and the emergence of these resistant bacteria has become a serious medical problem. Therefore, there has been a remarkable movement to restrict the use of antibiotics as animal feed additives in part.

[0004] In such a case, the intestinal effect of lactic acid bacteria,
It is considered to be a substitute for antibiotics due to the effect of improving the intestinal environment. For example, JP-A-55-9770 discloses a growth promoter containing lactic acid bacteria having antibiotic resistance as an active ingredient. The compound does not lose its activity even when used in combination with an antibiotic, prevents diarrhea caused by disturbance of the intestinal microflora due to the antibiotic, and white diarrhea caused by infection with Escherichia coli, and also has a growth promoting effect on livestock.

Further, Japanese Patent Application Laid-Open No. 49-123855 discloses a method of deodorizing animal feces by using it as a feed containing lactic acid bacteria having an odorless or deodorizing ability when cultured in raw feces or a diluted solution thereof. A method of doing so is disclosed.

As described above, attempts to add lactic acid bacteria to feed for domestic animals such as pigs have been made, and various effects have been achieved. However, in recent years, there has been a demand for a more superior feed that can improve the health of pigs and the like, improve nutritional efficiency, and the like.

[0007]

Therefore, the present invention can improve the health of domestic animals, especially pigs, without using antibiotics.
Another object of the present invention is to provide a feed additive capable of exhibiting a useful effect like the lactic acid bacteria.

[0008]

Means for Solving the Problems As a result of intensive studies, the present inventors have improved the health of pigs and improved their nutritional efficiency by using Lactobacillus casei cells as feed additives during breeding. And completed the present invention.

[0009] That is, the present invention according to claim 1 provides a feed supplement for swine raising which comprises Lactobacillus casei cells as an active ingredient.

[0010] Further, the invention according to claim 2 is a feed supplement for swine raising, comprising, as an active ingredient, Lactobacillus casei cells having an ability to enlarge swine cecum when taken orally. Is provided.

Further, the invention according to claim 3 is characterized in that Lactobacillus casei is Lactobacillus casei YIT-9.
The present invention provides a feed supplement for swine raising according to claim 1 or 2, which is 029 strain.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION The feed aid for swine raising of the present invention
By blending this in a feed, an intestinal regulation effect, a feed efficiency enhancing effect, and the like are provided. More specifically, by adding this, the amount of organic acids in feces can be increased,
Various physiological effects such as an increase in fiber absorption and a decrease in fecal pH can be obtained. These effects are considered to be due to the administration of the Lactobacillus casei cells, which enlarges the cecum of pigs, increases the content, and activates fermentation by intestinal bacteria.

[0013] For this reason, the cells of Lactobacillus casei used as a feed supplement are preferably those having higher cecal hypertrophy. That is, it is preferable that the cecal volume is increased by a factor of 2 or more, more preferably by a factor of 3 or more, by administering lactobacillus casei cells to pigs at 1 × 10 ¹⁰ cfu / day / head for 2 weeks or more. Is preferred.

The mechanism by which the cells of Lactobacillus casei enlarge the cecum is not clear, but the increase in the production of organic acids in the cecum leads to an increase in the proliferation of mucosal tissues,
It is considered that the cecal volume increased due to activation of tissue growth of the cecum itself.

The method for preparing the cells of Lactobacillus casei of the present invention is not particularly limited, and may be performed by a conventional method. For example, Lactobacillus casei proliferable MR
Culture is performed for about 24 hours in a medium such as S broth or GAM broth. Thereafter, the cells may be collected using a means such as centrifugation, but the culture solution may be used as it is.

When the cells thus recovered are blended into feed as an auxiliary, it is considered that the physiological effect can be enhanced by increasing the number of bacteria. However, in consideration of workability such as culture control of bacteria, cost, ease of administration, etc., the dose of Lactobacillus casei cells is 1 × per pig per day.
About 10 ¹⁰ cfu to 1 × 10 ¹² cfu, especially about 1 × 1
It is preferably about 0 ¹⁰ cfu to 1 × 10 ¹¹ cfu. Live cells may be used as they are, and treated with excipients such as glucose, sucrose, lactose, glutamic acid, ascorbic acid, skim milk, egg albumin, starch, casein, sucrose and freeze-dried. Alternatively, a highly conserved strain may be used. In the case of dead cells, it is preferable to use live cells because the physiological effects described above are low.

The feed supplement of the present invention may be orally administered alone, but is used as a powder or pellet feed together with various carbohydrates, proteins, lipids, fibers, vitamins and minerals. It is preferable to easily obtain the above-mentioned effects. In particular, when used for feed containing lipids and fibers, feed efficiency is improved, which is preferable for livestock management. At this time, the kind of lipid or fiber used is not particularly limited, and any fish, meat-and-bone meal, soybean meal, alfalfa meal, bran, etc. may be used as long as it can be used as a feed for swine raising.

Also, the production of the feed may be carried out by a conventional method, and more specifically, the feed may be mixed so as to have a fixed amount at the time of production.

[0019]

EXAMPLES The present invention will be described in more detail with reference to the following Examples, but it should not be construed that the invention is limited thereto.

[0020]

[Production Example 1] Production of feed supplement Lactobacillus casei YIT-9029 strain (FER
MBP-1366) into a 20% skim milk solution,
The cells were cultured at 37 ° C for 5 days. 80 parts by weight of an 18% glucose fructose solution was added to 20 parts by weight of the obtained culture solution to prepare a feed aid for pigs.

[0021]

Test Example 1 Administration Test The feed supplement of Production Example 1 was administered to 4 LW (Landrace × Large White) castrated pigs (25.3 ± 1.0 kg BW), and their physiological effects were examined. Feed is about 750g
Was given twice daily. The feed was prepared by mixing the components according to the following formula. The supplement was mixed with the feed when the feed was fed. The feed has a TDN (total digestible nutrient) of 73.
2%, DE (digestible energy) is 3456.4kcal /
kg.

(Feed prescription) Mixed feed 65.3% Soybean meal 18.7% Bran 3.7% Meat and bone meal 1.9% Alfalfa meal 9.3% Calcium carbonate 0.5% Salt 0.3% Vitamin ・Mineral premix 0.3%

The pigs were divided into two groups, A and B, each of which was subjected to an inversion test in which an experimental period consisting of a 2-week preliminary rearing period and a 1-week sampling period was repeated three times. Group A during the second experiment, Group B during the first and third experiments, 130 ml of feed supplement (Lactobacillus
Casei (at least 1 × 10 ¹⁰ cfu) was administered at the time of feeding.

In the sampling period, feces were collected at intervals of 20 minutes for 4 days, and the digestibility was measured. In addition, feces before feeding the feed on the sixth day of the sampling period were collected, and fecal pH,
The amount of organic acids in feces was measured.

The digestibility was measured by a general analysis method for the amounts of crude fat and crude fiber.

Crude fat: 2 g of dried and pulverized feces was placed in a cylindrical filter paper and stoppered with absorbent cotton. After drying at 100 ° C. for 2 hours, empty weight was measured in advance in a Soxhlet lipid extractor, and a corben containing 80 ml of ether was attached to the lower part of the extractor. The kolben was heated to 60 ° C., and the condenser was extracted with tap water for 16 hours. After removing the cylindrical filter paper and collecting the ether in the colben in an extractor, the corben was removed and wiped well. The corben was dried at 100 ° C. for 3 hours and weighed to determine the crude fat content.

Crude fiber: 1.2 g per 2 g of dried and ground feces
200 ml of 5% sulfuric acid was added and boiled. After boiling, the mixture was further boiled for 30 minutes, and 300 ml of distilled water was added. The mixture was left overnight and a precipitate was collected. 10 ml of distilled water and 50 ml of a 5% sodium hydroxide solution were added to the precipitate, and distilled water was further added to 200 ml. After boiling again, the mixture was filtered through a glass filter whose empty constant weight had been determined, and rinsed with distilled water and ethanol. It was dried at 135 ° C. for 2 hours and weighed to determine the amount of crude fiber.

The pH of feces was measured using a pH meter (COMPAC).
T pH METER twinpH; HORIBA)
Was measured by The measurement of the amount of the organic acid was performed as follows. The feces are diluted 3 times with distilled water and 1800 g, 4
After centrifugation at 10 ° C. for 10 minutes, the supernatant was collected. 1 ml of supernatant
Was added to 100 μl of 70% perchloric acid and left at 4 ° C. for 10 minutes or more to remove proteins. After centrifugation at 20,000 g at 4 ° C. for 10 minutes, the supernatant was filtered through a filter (0.45 μm).
Then, the organic acid was measured by liquid chromatography.

As a result, fecal pH was 6.7 ± in the control period.
0.5 and 6.0 ± 0.2 during the administration period, and significantly decreased during the administration period (there was a significant difference at a risk rate of 5%).

As shown in Table 1, the amount of organic acids in feces increased significantly during the administration period. Individually, the increases in acetic and propionic acids were significant.

[0031]

[Table 1]

The digestibility of crude fat and crude fiber was improved during the administration period (Table 2).

[0033]

[Table 2]

[0034]

[Test Example 2] Dissection Two LW castrated pigs were fed the above feed twice a day (at 10:00 and 22
Hour) and 750 g each. Feed supplementation for one of them
130ml (Lactobacillus casei 1 × 10 ¹⁰cf
more than u) at the time of feeding at 10:00 with the feed
did. After breeding for 2 weeks, the cecum is removed and the contents are weighed.
Measured (Table 3).

[0035]

[Table 3]

As a result, it was found that the cecal weight of the individual to which the feed supplement of Production Example 1 was added was increased. This increase in weight was due to an increase in cecal volume.

[0037]

According to the feed additive of the present invention, it is possible to improve the feed efficiency of pigs in pig farms and the like. Moreover, ingestion of the feed additive of the present invention lowers fecal pH and increases the amount of organic acids in feces, so that an intestinal control effect on pigs can be obtained.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Kazunari Ushida 5-15-101, Kashido-cho, Nishinomiya-shi, Hyogo F-term (reference) 2B005 EA01 2B150 AA03 AB01 AB03 AB20 AC06 DD12 DD26

Claims (3)

[Claims] Claims: 1. A feed supplement for pig farming comprising a cell of Lactobacillus casei as an active ingredient. 2. A feed supplement for swine raising comprising, as an active ingredient, Lactobacillus casei cells capable of enlarging swine cecum when taken orally. 3. The feed supplement for swine raising according to claim 1, wherein the Lactobacillus casei is Lactobacillus casei YIT-9029 strain.