JP2000264809A - Composition for growth regulation of plant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain the subject composition capable of remarkably improving a growth regulating action of a plant such as improvement of sugar content, growth promotion or the like even if the amount of application is small, originating from a natural product having a high safety and useful in the field of agriculture, forestry or the like by including an alga body of a specific fine algae and its extract or the like. SOLUTION: This composition preferably contains more than one kind of materials selected from an alga body of a fine algae belonging to Botryococcus such as Botryococcus braunii SI-8 strain, SI-30 strain or SI-55 strain, extract thereof and pulverized powder thereof preferably in the ratio of about 1-1000 ppm based on the total amount. Preferably a cultured alga body collected from the culture obtained by culturing a fine algae belonging to Botryococcus using carbon dioxide as a carbon source is used as the alga body to produce the composition. Preferably the composition is useful for improving sugar content of tomato, propagation promotion of root of a rice plant, weight increase promotion of a plant body, improvement of germinating rate or the like.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a composition for regulating plant growth and a method for producing the same, and more particularly, to a remarkable plant growth regulating effect using microalgae belonging to the genus Botryococcus. The present invention also relates to a composition for controlling plant growth which is highly safe and is useful in the fields of agriculture, forestry, fertilizer and agrochemical industry, and a method for producing the same.

[0002]

2. Description of the Related Art At present, in the field of agriculture and forestry, a large amount of chemically synthesized materials are used as fertilizers or pesticides, which cause residual soil toxicity, continuous cropping failure, destruction of the natural environment, water sources. Pollution has become a major problem.

[0003] On the other hand, food problems and global environmental problems are about to become urgent issues for human beings, and while having a plant growth regulating effect effective for increasing food production and securing forest resources, the above-mentioned problems occur. There was a need for materials for regulating plant growth. Therefore, as safe plant growth regulators derived from natural products developed to satisfy this demand, chlorella extracts and euglena extracts (JP-A-4-35270) have been used.
No. 5) has been known. However, these conventional plant growth regulators do not show a remarkable effect in a very small amount, and it is hard to say that they have high plant growth regulating activity.

[0004]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above point of view, and it is intended to remarkably improve plant growth regulating effects such as improvement in sugar content and promotion of growth even when the amount of application is very small. It is an object of the present invention to provide a composition for regulating plant growth derived from natural products, which is safe and highly safe.

[0005]

Means for Solving the Problems In order to solve the above-mentioned problems, the present inventors have conducted intensive studies on the plant growth regulating effect of a safe composition derived from a natural product. As a result, the present invention belongs to the genus Botryococcus. The present inventors have found that algal bodies of microalgae have an excellent plant growth regulating action, and have completed the present invention.

That is, the present invention is as follows.

(1) Botryococcus
A composition for regulating plant growth comprising one or more selected from the alga body of microalgae belonging to the genus, an extract of the alga body, and a crushed product of the alga body. (2) The composition according to (1), wherein the extract is extracted from algae of microalgae with an oxygen-containing organic solvent or a mixed solvent of an oxygen-containing organic solvent and another solvent.

(3) The microalgae belonging to the genus Botryococcus are Botryococcus brownies.
s braunii). (4) The composition according to (3), wherein the Botryococcus brownie is a Botryococcus brownie SI-8 strain, SI-30 strain, or SI-55 strain.

(5) The composition according to (1), wherein the plant is a tomato and the growth control is improvement in sugar content. (6) the plant is rice, and the growth regulation is promotion of root elongation;
The composition according to (1), which is selected from promoting plant weight increase and improving germination rate.

(7) The composition according to (1), wherein the plant is cabbage or lettuce, and the growth of the plant is promoted by increasing the weight of the plant. (8) The composition according to (1), wherein the plant is a microalgae and the growth is regulated by promoting the growth rate.

(9) The composition according to (8), wherein the microalgae is a green algae or a cyanobacterium. (10) alga bodies of microalgae belonging to the genus Botryococcus,
A method for producing a composition for regulating the growth of a plant selected from the extract of the alga body, and the crushed product of the alga body, wherein the alga body comprises a microalga belonging to the genus Botryococcus, wherein carbon dioxide is used. A method comprising culturing as a source and using cultured alga bodies collected from the obtained culture.

[0012]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail. <1> Microalgae belonging to the genus Botryococcus As the microalgae belonging to the genus Botryococcus used in the present invention (hereinafter, also referred to as “botaliococcus algae”), the alga itself and the alga body are used. Any extract or microalgae belonging to the genus Botryococcus in which the crushed algal body has a plant growth regulating action can be used without particular limitation.

The microalgae belonging to the genus Botryococcus include the Culture Collection of Algi and Protozoa as long as they are the microalgae of the genus Botryococcus having the above action.
gae and Protozoa), any of strains available from microorganism preservation institutions, wild strains inhabiting lakes and marshes in nature, strains isolated and purified from wild strains, and mutants thereof are used in the present invention. be able to.

Specific examples of such a microalga of the genus Botryococcus include Botryococcus brownies and the like. Examples of the Botryococcus brownies include, for the above-mentioned stocks, Botryococcus brownies CCAP807 and the like. Also,
The strains isolated by the present inventors from lakes and marshes are Botryococcus brownie SI-8 strain, Botryococcus sp.
Brownie SI-30 strain, Botryococcus brownie SI-55 strain and the like can be mentioned, and these strains are particularly preferably used in the present invention.

As for the method for separating microalgae belonging to the genus Botriococcus from lakes and marshes in the natural world, for example, the method for obtaining the Botulinococcus brownie SI-8 strain preferably used in the present invention is described in JP-A-10-150.
No. 995, Botryococcus Brownie SI
The method for obtaining -30 strains is described in JP-A-9-234055.
References can be referred to in the publications.

Botulio Coccus Brownie S
The I-55 strain is a new strain isolated from a lake in Hokkaido by the present inventors, and was identified as Botryococcus brownie by observing the colony shape and the like, as shown in the Examples described later.

The method for separating microalgae belonging to the genus Botryococcus will be described in more detail. For example, first, a botulinum existing on a lake surface such as a freshwater lake (including a pond and a swamp) and a brackish lake is described. Coccus algae are collected as a botulinococcus algae-containing sample containing impurities using a plankton net or the like. Confirmation of the presence of Botryococcus algae in a sample collected by the plankton net or the like is performed by using a microscope to observe the presence of a colony having a morphology characteristic of Botryococcus algae. Can be performed.

Since the sample containing the Botryococcus algae collected as described above generally contains microalgae other than Botryococcus algae, microorganisms such as bacteria or fungi, the sample is subjected to a conventional method. According to chlorine sterilization,
By purifying by plate culture or the like, various purified strains of Botryococcus algae can be obtained.

Each of the various purified strains of Botryococcus algae obtained in this manner is further subjected to liquid or solid cultivation in a medium suitable for culturing Botryococcus algae in a liquid or solid state according to a conventional method. The target purified strain can be obtained by analyzing the body by a conventional method and examining each characteristic.

The above-mentioned strains of Botryococcus brownie SI-8 strain, Botryococcus brownie SI-30 strain, and Botryococcus brownie SI-55 strain isolated by the present inventors from lakes and marshes were used. Deposit with the National Institute of Bioscience and Human-Technology, National Institute of Advanced Industrial Science and Technology has been refused, but the transfer of these strains to confirm the establishment of the present invention is guaranteed here.

<2> Algae, algal extract and crushed alga of microalga belonging to the genus Botryococcus The present invention is obtained by culturing the microalgae belonging to the genus Botryococcus as described above. Cultured alga bodies are used.

The method for culturing the microalga belonging to the genus Botryococcus is not particularly limited as long as the obtained alga itself or an extract or crushed alga body has a plant growth regulating action. For example, a method of autotrophically culturing carbon dioxide (CO ₂ ) under a photosynthetic condition under irradiation of sunlight or artificial light using carbon dioxide (CO ₂ ) as a carbon source, and a heterotrophic culture using glucose or the like as a carbon source under dark conditions And the like, but autotrophic culture is preferred. The cultivation of the aforementioned Botryococcus algae can be performed by liquid culture or solid culture.

Specifically, using an inorganic medium such as a CHU-13 × 2 medium or a JM medium described below, the temperature is controlled at 5 to 50 ° C., and under light irradiation, contains approximately 20% of CO ₂ . A method such as culturing while aerating air is used.

As the composition of the present invention, any alga body of a microalga belonging to the genus Botryococcus, or an extract or crushed product thereof can be used. Moreover, the alga body, the extract, and the crushed product may be used alone or in any mixture.
Furthermore, algal bodies of an arbitrary plurality of strains of microalga belonging to the genus Botryococcus, extracts or crushed products thereof can also be used.

The alga bodies can be recovered from the culture solution by filtration, centrifugation, or the like. The collected alga bodies may be used as they are, or may be freeze-dried or dehydrated with acetone-dry ice or the like.

The extraction method of the algal body is not particularly limited as long as it has a growth regulating effect on plants. Suitable solvents for use in the extraction include oxygenated solvents or mixtures of oxygenated solvents with other solvents. Examples of the oxygen-containing solvent include alcohols such as ethanol and methanol, ketones such as acetone, and ethers such as diethyl ether. Other solvents other than the oxygen-containing solvent include chloroform and the like. The extraction solvent comprising the oxygen-containing solvent or a mixture of the oxygen-containing solvent and another solvent may contain water. Prior to extracting algal cells with an oxygen-containing solvent or a mixture of an oxygen-containing solvent and another solvent, unnecessary fractions are removed by pre-extraction with a solvent such as n-hexane or petroleum ether. Is also good. The amount of the solvent used, the number of extractions, and the like are not particularly limited. The obtained extract may be appropriately concentrated or diluted.

The crushing method for obtaining the crushed algae is not particularly limited as long as a uniform solution or suspension can be formed due to the cell contents flowing out by crushing, and the like. Homogenizer, ball mill, French press, ultra Mechanical methods such as sonication can be used. When crushing algae,
It may be effective to add a surfactant to the algal cells. As surfactants, polyoxyethylene alkyl ethers, polyoxyethylene alkyl aryl ethers,
Lignin sulfonate and the like.

<3> The composition for controlling growth of a plant of the present invention The composition for controlling growth of the present invention comprises an algal body of a microalga belonging to the genus Botryococcus as described above, or an extract or crushed product thereof. .

The above alga, extract or crushed product can be used as it is as a composition for regulating plant growth, but it can be used together with components such as a carrier, a dispersant, a spreading agent and a surfactant to prepare a formulation. It can also be converted. There is no particular limitation on the formulation method. The dosage form is not particularly limited, and may be any of a liquid preparation, a powder, and a solid preparation. As the carrier, any of organic and inorganic substances can be used, and those containing both organic and inorganic substances may be used. Specifically, for example, akadama soil, calcined akadama soil, Kanuma soil, ando soil, vermiculite, perlite, zeolite and other inorganic substances, or peat moss, charcoal, pulp, straw, bagasse, oil residue,
Organic substances such as fish meal, bone meal, blood meal, shell fossils, crab chunks, and the like, and mixtures thereof.

The dispersant, spreading agent and surfactant can be used without any particular limitation as long as they are commonly used in formulating a composition for controlling plant growth.

The mixing ratio of alga, extract or crushed product in the total amount of the composition is not particularly limited, but is usually 0.01 to 1
0000 ppm, preferably about 1 to 1000 ppm.

The method of using the composition for controlling growth is not particularly limited. In the case of a liquid preparation, the composition is appropriately diluted,
It may be applied by spraying on a plant or soil, or immersing a plant or its seed. In the case of a powder or a solid preparation, the preparation may be applied as it is or after dispersing it in a suitable solvent in the same manner as the liquid preparation. Furthermore, when a plant is cultivated and transplanted from a nursery to the main field, it may be used for either the nursery or the main field, or may be used for both.

The growth-regulating composition of the present invention is not particularly limited in the type of plant to which it can be applied.
It can be used for cruciferous plants such as cabbage or lettuce, solanaceous plants such as tomato, asteraceae plants, fruit trees, and the like, and has effects such as promoting growth, promoting germination, and improving sugar content. In particular, tomato has improved sugar content, rice has at least one of root elongation promotion, plant weight increase promotion or germination rate improvement, and cabbage or lettuce has growth promotion,
In particular, promotion of increase in plant weight can be expected.

Plants to which the composition for controlling growth of the present invention can be applied further include green algae such as chlorella and Chlamydomonas and microalgae such as cyanobacteria. When the composition of the present invention is applied to these microalgae, they exhibit effects such as promotion of the growth rate. The method for using the composition for regulating growth of the present invention in the microalgae is not particularly limited as in the case of the other plants described above. For example, the composition may be contained at a suitable concentration in a growth medium or the like of the microalgae. Is used. In addition, cyanobacteria are often referred to as cyanobacteria in recent years and are often classified as a kind of bacteria, but in this specification, this is described in the term "cyanobacteria" and included in the category of plants. It was taken.

[0035]

The present invention will be described in more detail with reference to the following examples. First, Botryococcus Brownie SI-
A production example of 55 strains will be described.

[0036]

[Production Example] Botryococcus Brownie SI-5
The planktons on the surface of the lake in Hokkaido were collected using a mountain root-type plankton net having a network of 25 μm with 5 strains. When the obtained lake water sample was observed with a microscope, colonies having a morphology characteristic of Botryococcus algae were observed. This lake water sample was divided into four test tubes, each having an effective chlorine concentration of 1 ppm, 5 ppm, and 1 ppm.
Sodium hypochlorite was added at 0 ppm and 20 ppm, and the mixture was allowed to stand at room temperature for 10 minutes. After standing, add 1m of active chlorine to remove sodium thiosulfate to remove active chlorine.
It was added to give 7.16 mg / g. Then, the above-mentioned treatment liquid was added to J containing 0.1% of glucose and 1.5% of agar.
M medium, and apply about 15 μE / m ² ·
The cells were cultured at 25 ° C. for 1 week while irradiating with light so as to reach s.

After the above culture, a single colony of Botryococcus algae was subjected to platinum plating while observing the unique properties of Botryococcus algae such as colony shape using a stereoscopic microscope (magnification 5 to 60) in a clean bench. It was scraped off with a line and transplanted to a JM plate medium containing 0.1% of glucose and 1.5% of agar. This was cultured at 25 ° C. for 4 weeks under light irradiation of about 15 μE / m ² · s by a fluorescent lamp.

The algae of Botryococcus algae obtained above were scraped off using a platinum wire and transferred to a JM liquid medium.
The cells were further cultured under light irradiation for 2 weeks. Take a part of the culture solution with a platinum loop and use a normal agar medium (meat extract: 5 g in 1 L,
Peptone: 10 g, sodium chloride: 5 g, agar: 15
g; pH 7.0), and cultured at 25 ° C. for 7 days to confirm that no bacteria grow. In addition, microscopic observation of the purified strain obtained in this way revealed that only Botryococcus brownies showed the unique properties of the algae, such as colony shape (a pear-shaped cell containing chloroplasts in the cells was collected. An approximately spherical colony was formed, and the colony was further observed under a microscope to produce oil droplets.) The obtained strain was designated as Botryococcus brownie SI-55 strain.

The lake water sample was used as it was on a flat plate (JM
The medium was coated and cultured on a JM medium supplemented with a medium and glucose, but no colonies of only Botryococcus algae were obtained.

Next, the Botryococcus brownie SI-55 strain obtained above was inoculated into a JM medium containing glucose, and cultured for 2 months while repeating subculture. The grown alga bodies were inoculated into a flat flask containing 0.3 L of CHU-13 × 2 medium, and cultured at 25 ° C. for 8 days while blowing 5% CO ₂ (0.5 vvm). The algae obtained by the culture were dried at 105 ° C. After adding 1 mg of n-pentacosane as an internal standard to the dried algal cells, n
-Extracted three times with hexane. After collecting and concentrating the n-hexane phases for three times, gas chromatography, GC-M
Analyzed by S. A phenylmethylsilicon-based capillary column (25 m) was used for gas chromatography. As a result, it was analyzed that the content of the linear olefin having 27, 29, and 31 carbon atoms in the n-hexane extract component of the cultured alga was 15.2%.

The composition of the JM medium and the composition of the CHU-13 × 2 medium used above are as follows.

[Composition of JM medium] 1 mL of each of the stock solutions 1 to 9 in Table 1 below is mixed, adjusted to 1 L with distilled water or deionized water, and the pH is adjusted to around 7.

[0043]

[Table 1] [Composition of CHU-13 × 2 medium] After mixing the components shown in Table 2 below, the pH is adjusted to around 7.

[0044]

[Table 2]

[0045]

[Table 3]

[0046]

Example 1 The Botryococcus sp.
Algae extracts were prepared using the Brownie SI-55 strain and the Botryococcus brownie SI-8 strain and the Botryococcus brownie SI-30 strain separately obtained by the present inventors.

That is, the Botryococcus brownie SI-8 strain was inoculated into a flat flask (0.3 L) containing 0.3 L of CHU-13 × 2 medium and subjected to liquid culture (illuminance) for 8 days. : 350 μE / m ² · s, aeration rate of 5% CO ₂ : 0.5 vvm, temperature: 25 ° C). The obtained culture was filtered through a nylon filter cloth having a mesh of 25 μm to separate into an alga body and a culture solution. The obtained alga bodies were dried by freeze-drying, and then extracted three times with n-hexane. The alga bodies after the n-hexane extraction were extracted three times with a mixed solution of chloroform and methanol (1: 1). The extract of chloroform and methanol was concentrated under reduced pressure to obtain an extract. Similarly, SI
Extracts of −30 strains and SI-55 strains were obtained.

[0048]

Example 2 Koshihikari seeds were sterilized with a 2% sodium hypochlorite solution for 15 minutes, washed five times with water, then immersed in water, absorbed at 25 ° C. for 20 hours, drained and washed at 25 ° C. at 24 ° C. After germination for a long time, germination germs used in a petri dish test were obtained.

The petri dish test was conducted using a plant glass container φ40.
5 mL of agar medium was dispensed and solidified using × 100 mm (manufactured by Shibata), and 5 seeds of germinating firs / petal were placed at equal intervals on it, and cultivated in the dark at 30 ° C. for 3 days. went.

The Botryococcus brownie SI-8 strain obtained in Example 1 and the Botryococcus brownie S.
I-30 strain and Botryococcus brownie SI
Each of the extracts (chloroform / methanol (1: 1) extract) of -55 strain was added with polyoxyethylene dodecyl ether, calcium ligninsulfonate and polyoxyethylene nonylphenyl ether in a total amount of 0.055.
A surfactant solution containing 1% by volume has a concentration of the extract of 5%.
ppm and 50 ppm, which were subjected to a petri dish test to evaluate the elongation promoting effect on roots.

After cultivation in a dark place at 30 ° C. for 3 days, 15
Measure the root length of the sample / test and, for these measurements,
A T-test was performed on a control without addition (only a surfactant solution was added), and a significant root elongation promoting effect was confirmed at a risk factor of 5%.

The results are shown in Table 4. The extract of the strain SI-8 was at a concentration of 50 ppm, and the extract of the strains SI-30 and SI-55 was 5 ppm and 50 ppm. Was done.

[0053]

[Table 4] Table 4 Botryococcus plauuni-extract Concentration Root elongation promotion rate T test ──────────────────────────────────── SI-8 strain 50ppm 1.49 Danger There is a significant difference at a rate of 5% Extract 5ppm 1.23 No significant difference ──────────────────────────────────── SI-30 strain 50 ppm 1.50 Significant difference at 5% risk Extract 5 ppm 1.28 Significant difference at 5% risk ──────────────────────── ──────────── SI-55 strain 50 ppm 1.48 Significant difference at 5% risk Extract 5 ppm 1.38 Significant difference at 5% risk ──────────── ──────────────────────── No additive 1.00 −−− ─────────── ─────────────────────────

[0054]

Example 3 In the same manner as in the petri dish test of Example 2, after Koshihikari firs were sterilized, TOYO was added to a glass petri dish.
No. 7 Filter paper was spread and 2 mL of the test solution was dispensed.
After leaving in a dark place, the germination rate was measured and compared with the control without addition. In this test, a surfactant solution containing each algal extract obtained in the same manner as in Example 2 was used as the test solution.

Table 5 shows the results.
Each extract of -30 strain and SI-55 strain was 5 ppm and 5 ppm.
A germination promoting effect was recognized at 0 ppm.

[0056]

[Table 5] Table 5 ──────────────────────── Botryococcus plauuni extract concentration Germination rate ────────── ────────────── SI-8 strain 50ppm 0.81 extract 5ppm 0.93 ──────────────────────── SI- 30 strains 50ppm 0.83 extract 5ppm 0.93 ──────────────────────── SI-55 strain 50ppm 0.82 extract 5ppm 0.83 ──────── ──────────────── No additive 0.70 ────────────────────────

[0057]

Example 4 In the same manner as in the Petri dish test of Example 2, the germinated firs of Koshihikari, which had been subjected to seed sterilization and germination treatment, were spread with gauze on a perforated plastic pad floating in water.
In an artificial weather device (Koito Kogyo: HNL-35DA)
10,000 lux, 25 ° C, 12 hours daytime, 20 ° C, 12 hours
The seedlings were obtained by cultivating for 3 weeks alternately under dark conditions of time.

The hydroponic cultivation was carried out by transplanting the seedlings into a foamed styrene plate floating in a hydroponic solution and cultivating them. Three seedlings of Koshihikari were used, and the fir portions were sponge pieces (10
× 20 × 50 mm), inserted into a styrene foam plate (20 × 63 × 256 mm) having a hole (φ20 mm), and floated in a hydroponic solution. The cultivation was carried out in an artificial weather vessel under the same light and temperature conditions as the seedlings, and the hydroponic solution was replaced every 2-3 days using Hyponex (manufactured by Murakami Sangyo) 5,000-fold solution.

Each extract obtained in the same manner as in Example 1 was emulsified and dispersed by adding a surfactant solution in the same manner as in Example 2 except that the concentration of the extract was changed to 10 ppm and 50 ppm. Two times, one week and one week later, the seedlings were applied to the entire foliage of the seedlings and compared with the control without additives. After cultivation for three weeks after transplantation, each seedling was taken out, dried at 110 ° C. for 4 hours, and weighed.

Control of no addition (meaning only surfactant solution) for the measured dry weight of each of 15 samples / test
Was performed, and a significant dry weight increasing effect (growth promoting activity) was confirmed at a risk factor of 5%.

The results are shown in Table 6. The extract of the SI-8 strain was 50 ppm, and the extract of the SI-30 strain and the extract of the SI-55 strain were 10 ppm and 50 ppm. ) Was observed.

[0062]

[Table 6] [Table 6] Botryococcus plaunie extract concentration Weight increase promotion rate T test ──────────────────────────────────── SI-8 strain 50ppm 1.22 Risk factor 5% with significant difference Extract 10ppm 1.09 No significant difference ──────────────────────────────────── SI -30 strains 50ppm 1.24 Significant difference at 5% risk Extract 10ppm 1.15 Significant difference at 5% risk ───────────────────────── ─────────── SI-55 strain 50 ppm 1.31 Significant difference at 5% risk Extract 10 ppm 1.17 Significant difference at 5% risk ───────────── ─────────────────────── No additive 1.00 −−− ──────── ───────────────────────────

[0063]

Example 5 A germination pad of Kinuhikari (350 ml / box) obtained in the same manner as in the petri dish test of Example 2 was cultivated in a house in a nursery box (300 × 600 mm) containing Sankyo granular soil.

The SI-30 strain obtained in the same manner as in Example 1,
To each extract of the strain SI-55, a surfactant solution was added and emulsified and dispersed in the same manner as in Example 4 to obtain 50 ml / box.
Spraying was applied to the entire foliage of the seedlings at the leaf stage to compare with the no-addition control. After cultivation for 10 days from the spraying treatment, each seedling was taken out, dried at 110 ° C. for 4 hours, and weighed.

For each of the measured values of the dry weight of 15 samples / test, a T sample was used for the control to which no additive was added, and a significant dry weight increasing effect (growth promoting activity) was confirmed at a risk factor of 5%.

The results are shown in Table 7. The extract of the SI-30 strain and the SI-55 strain showed a significant effect of increasing dry weight (growth promoting activity) at 50 ppm.

[0067]

[Table 7] Table 7: Botryococcus plauuni extract concentration Weight increase promotion rate T test ──────────────────────────────────── SI-30 strain 50ppm 1.53 Risk factor There is a significant difference at 5% Extract 10 ppm 1.32 No significant difference ──────────────────────────────────── SI -55 strain 50ppm 1.60 Significant difference with a 5% risk factor Extract 10ppm 1.56 No significant difference ────────────────────────────── ────── No additive 1.00 −−− ────────────────────────────────────

[0068]

Example 6 Cabbage (Kinkei No. 201) was cultivated in a house in a 128-well cell tray (manufactured by Yanmar) containing cultivated soil for cell molding seedlings.

In each extract obtained in the same manner as in Example 1, the concentration of the extract was 5 ppm, 10 ppm, 50 ppm,
A surfactant solution was added and emulsified and dispersed in the same manner as in Example 2 except that the amount was set to 00 ppm. Only added). After cultivation for 14 days from the spraying treatment, each seedling was taken out and 11 days after the cultivation.
After drying at 0 ° C. for 4 hours, the weight was measured.

The measured value of the dry weight of each of 15 samples / test was subjected to a T test against the control without addition, and a significant effect of increasing the dry weight (growth promoting activity) was confirmed at a risk factor of 5%.

The results are shown in Table 8, where the extract of the SI-8 strain was 10 to 100 ppm and the extract of the SI-30 strain was 5 ppm.
ppm and 100 ppm, the extract of strain SI-55
A significant increase in dry weight (growth promoting activity) was observed at pm to 100 ppm.

[0072]

[Table 8] Table 8: Botryococcus plauuni extract concentration Weight increase promotion rate T test ──────────────────────────────────── SI-8 strain 100ppm 1.15 Risk factor There is a significant difference at 5% Extract 50ppm 1.38 Significant difference at 5% risk 10ppm 1.38 Significant difference at 5% risk 5ppm 1.02 No significant difference ───────────────── ─────────────────── SI-30 strain 100 ppm 1.43 Significant difference at 5% risk Extract 50 ppm 1.06 No significant difference 10 ppm 1.04 No significant difference 5 ppm 1.25 Risk factor 5 % Significant ──────────────────────────────────── SI-55 strain 100 ppm 1.30 Risk factor 5 Extraction 50 ppm 1.19 Risk factor 5% Significant difference 10 ppm 1.30 Risk factor 5% Significantly different 5ppm 1.23 Significantly different at 5% risk ──────────────────────────────────── None Addition 1.00 −−− ────────────────────────────────────

[0073]

Example 7 Lettuce (Salinas 88) was cultivated in a house with a 200-well cell tray (manufactured by Yanmar) containing cultivation soil for cell molding seedlings.

To each extract obtained in the same manner as in Example 1, a surfactant solution was added and emulsified and dispersed in the same manner as in Example 6, and 100 ml / tray was sprayed on the entire foliage of the two-leaf seedling. It was sprayed and compared with a control without addition.
After cultivation for 14 days from the spraying treatment, each seedling was taken out and kept at 110 ° C.
For 4 hours and weighed.

The control value of no addition (only the surfactant solution was added) for the measured values of the dry weight of each of 15 samples / test
Was performed, and a significant dry weight increasing effect (growth promoting activity) was confirmed at a risk factor of 5%.

The results are shown in Table 9, where the extract of strain SI-8 was 5 to 50 ppm and the extract of strain SI-30 was 5 to 5 ppm.
At 0 ppm, the extract of strain SI-55 was 5 ppm, 50 p
A significant increase in dry weight (growth promoting activity) was observed at pm and 100 ppm.

[0077]

[Table 9] Table 9: Botryococcus plauuni extract concentration Weight increase promotion rate T test ──────────────────────────────────── 100ppm 1.04 No significant difference SI-8 Strain 50 ppm 1.21 Significant difference at 5% risk Extract 10 ppm 1.26 Significant difference at 5% risk 5 ppm 1.23 Significant difference at 5% risk ───────────────── ─────────────────── 100 ppm 1.08 No significant difference SI-30 strain 50 ppm 1.14 Significant difference at 5% risk Extract 10 ppm 1.18 Significant difference at 5% risk ratio 5ppm 1.16 Significant difference at 5% risk rate ──────────────────────────────────── 100ppm 1.17 Risk rate Significant difference at 5% SI-55 strain 50ppm 1.20 Significant difference at 5% risk factor Extract 10ppm 1.0 7 No significant difference 5ppm 1.11 Significant difference at 5% risk ──────────────────────────────────── No additive 1.00 −−− ────────────────────────────────────

[0078]

Embodiment 8 Botryococcus brownie SI-5
Five strains were cultured, filtered and dried in the same manner as in Example 1. The obtained alga bodies were extracted three times with a 99.5% ethanol aqueous solution or a 70.0% ethanol aqueous solution,
It was concentrated under reduced pressure. These ethanol aqueous extracts were added to the same surfactant solution as in Example 2 at 5 ppm, 50 ppm,
The procedure was performed in the same manner as in Example 2 except that the mixture was added at a concentration of 100 ppm and subjected to a petri dish test.

The results are shown in Table 10, and a significant root elongation promoting activity at 100 ppm was observed for both the 99.5% aqueous ethanol extract and the 70.0% aqueous ethanol extract.

[0080]

Table 10 抽出 Extract Extract concentration Root elongation promotion Rate T test ──────────────────────────────────── 99.5% ethanol 100ppm 1.27 Significant at 5% risk Extracted 50 ppm 1.18 No significant difference 5 ppm 1.12 No significant difference ──────────────────────────────────── 70.0% ethanol 100ppm 1.33 Significant difference at 5% risk Extract 50ppm 1.12 No significant difference 5ppm 1.07 No significant difference ───────────────────────── ─────────── No additive 1.00 −−− ───────────────────────────────── ───

[0081]

Example 9 The same surfactant solution as in Example 2 was added to the dried algal cells of the strain SI-55 obtained in Example 1, and crushed with a French press or a pressurized homogenizer. A test was performed in the same manner as in Example 2 except that the obtained crushed product was added to the same surfactant solution as in Example 2 at the following concentration and subjected to a petri dish test.

The results are shown in Table 11. The crushed French press and the crushed pressurized homogenizer were as follows.
A significant root elongation promoting activity was observed at 100 ppm and 200 ppm.

[0083]

[Table 11] Table 11 ─────────────────────────────────── Crushed material Crushed material concentration Root elongation promotion rate T test ─────────────────────────────────── French press 200ppm 1.19 Significant difference with 5% risk factor 100ppm 1.20 Significant difference at 5% risk factor 加 圧 Pressurized 200ppm 1.23 Risk factor Homogenizer 100 ppm 1.15 Significant difference at 5% with significant difference 危 険── No additive 1.00 −−− ───────────────────────────────────

[0084]

Example 10 Tomato (Momotaro T93) was grown in a glass greenhouse. Irrigation was performed almost daily. Fruit setting was performed by tomato tone treatment. Pinching was performed as soon as two leaves after flowering of the sixth inflorescence emerged. Each inflorescence was restricted to no more than 3 fruits.

A chloroform / methanol extract of the SI-55 strain obtained in the same manner as in Example 1 was added to the same surfactant solution as in Example 2 at a concentration of 10 ppm, from the flowering stage of the first flower cluster to the first flower cluster. It was sprayed four times approximately every 10 days until harvest. As a result of measuring the sugar content of the obtained tomato, in the third and fourth flower clusters, 5% was obtained as compared with the untreated section.
The sugar content was significantly higher at the risk factor.

[0086]

Embodiment 11 Chlamydomonas reinhardti (Ch)
lamydomonas reinhcrditii) 0.3 L of IAMC-9 strain
(Eversole medium: the composition is shown in Table 12 below). To this, a chloroform / methanol extract of the SI-55 strain obtained in the same manner as in Example 1 was added so as to be 1 ppm, 10 ppm, and 100 ppm, and cultivation for 4 days was performed with no addition as a control (illuminance:
150 μE / m ² · s, temperature: 25 ° C., ventilation volume: 0.3
vvm (5% CO ₂ )). After completion of the culture, the algal body concentration in each culture solution was measured.

The results are shown in Table 14, where the extract concentration was 1p
At 10 ppm, the effect of promoting the growth rate of algal cells was observed.

[Composition of Eversole medium]
After mixing the components in 2, adjust the pH to 8.3 (Ev
ersole. RA: Am. J. Botany, 43, 404 (1956)).

[0089]

[Table 12]

[0090]

[Table 13]

[0091]

[Table 14]

[0092]

Comparative Example 1 Chlorella vulgaris I was used in place of the algal extract of Botryococcus braunii (chloroform / methanol (1: 1) extract).
An algal cell extract (chloroform / methanol (1: 1)) obtained by treating the algal cells of AMC-27 in the same manner as in Example 1.
1) Extraction was carried out in the same manner as in Example 2, except that no root elongation promoting effect was observed.

[0093]

Comparative Example 2 The culture of the Botryococcus brownie SI-8 strain obtained in Example 1, ie, the culture of the alga body of Example 1 was filtered to remove the algal cells of the strain. A test similar to that of Example 2 was performed using the same, but no effect of promoting root elongation was observed.

[0094]

Industrial Applicability The composition for regulating plant growth of the present invention uses algal bodies of microalga belonging to the genus Botryococcus, an extract or crushed product thereof, and has a remarkable and small amount of plant growth regulating action ( Root elongation accelerating action, plant weight increase accelerating action, germination rate improving action) and high safety. Further, according to the present invention, contribution to increasing food production and securing forest resources by regulating plant growth, and global environmental problems and energy problems due to CO ₂ fixation and hydrocarbon (fuel) production by culturing algae belonging to the genus Botryococcus Can be contributed.

Claims (10)

[Claims] 1. A method for regulating the growth of a plant containing one or more selected from the group consisting of microalgae alga bodies, extracts of the alga bodies, and crushed alga bodies belonging to the genus Botryococcus. Composition. 2. The composition according to claim 1, wherein the extract is extracted from algal bodies of microalgae with an oxygen-containing organic solvent or a mixed solvent of an oxygen-containing organic solvent and another solvent. 3. The microalgae belonging to the genus Botryococcus is Botryococcus brownii.
aunii). 4. The Botryococcus brownie,
Botulio Coccus Brownie SI-8, SI-3
The composition according to claim 3, which is 0 strain or SI-55 strain. 5. The composition according to claim 1, wherein the plant is a tomato, and the growth control is improvement in sugar content. 6. The composition according to claim 1, wherein the plant is rice, and the growth control is selected from promotion of root elongation, promotion of increase in plant weight, and improvement of germination rate. 7. The plant is cabbage or lettuce,
2. The composition according to claim 1, wherein the growth is regulated by promoting plant weight increase. 8. The composition according to claim 1, wherein the plant is a microalgae, and the growth is regulated by a growth rate. 9. The composition according to claim 8, wherein the microalgae is a green algae or a cyanobacterium. 10. A method for producing a composition for controlling plant growth selected from alga bodies of microalgae belonging to the genus Botryococcus, extracts of the alga bodies, and crushed products of the alga bodies, wherein the method comprises: A method characterized in that microalgae belonging to the genus Botryococcus are cultured using carbon dioxide as a carbon source, and the cultured alga bodies recovered from the resulting culture are used as the algal bodies.