JP2000221179A5 - - Google Patents
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- JP2000221179A5 JP2000221179A5 JP1999022482A JP2248299A JP2000221179A5 JP 2000221179 A5 JP2000221179 A5 JP 2000221179A5 JP 1999022482 A JP1999022482 A JP 1999022482A JP 2248299 A JP2248299 A JP 2248299A JP 2000221179 A5 JP2000221179 A5 JP 2000221179A5
- Authority
- JP
- Japan
- Prior art keywords
- liquid chromatography
- analysis liquid
- anion analysis
- crosslinked polymer
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 238000004458 analytical method Methods 0.000 description 8
- 150000001450 anions Chemical class 0.000 description 8
- 238000004811 liquid chromatography Methods 0.000 description 8
- 239000002245 particle Substances 0.000 description 7
- 229920000642 polymer Polymers 0.000 description 6
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 5
- 238000010557 suspension polymerization reaction Methods 0.000 description 5
- 239000007900 aqueous suspension Substances 0.000 description 4
- 150000001252 acrylic acid derivatives Chemical class 0.000 description 3
- CERQOIWHTDAKMF-UHFFFAOYSA-M methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 125000001453 quaternary ammonium group Chemical group 0.000 description 2
- AOBIOSPNXBMOAT-UHFFFAOYSA-N 2-[2-(oxiran-2-ylmethoxy)ethoxymethyl]oxirane Chemical compound C1OC1COCCOCC1CO1 AOBIOSPNXBMOAT-UHFFFAOYSA-N 0.000 description 1
- SHKUUQIDMUMQQK-UHFFFAOYSA-N 2-[4-(oxiran-2-ylmethoxy)butoxymethyl]oxirane Chemical compound C1OC1COCCCCOCC1CO1 SHKUUQIDMUMQQK-UHFFFAOYSA-N 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- BRLQWZUYTZBJKN-UHFFFAOYSA-N epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Description
【特許請求の範囲】
【請求項１】 水酸基を持つアクリレート及びメタクリレート系架橋性モノマーからなる群より選ばれる１種または２種以上のモノマーを水性懸濁重合し、得られた架橋ゲルの水酸基を利用してスペーサーを介し４級アンモニウム基が導入されてなる陰イオン分析液体クロマトグラフィー用架橋重合体粒子。
【請求項２】 スペーサーがエピクロルヒドリン、エチレングリコールまたは１，４−ブタンジオールジグリシジルエーテルまたはエステル、ポリエチレングリコールジグリシジルエーテルまたはエステルの残基のいずれかである請求項１に記載の陰イオン分析液体クロマトグラフィー用架橋重合体粒子。
【請求項３】 粒径が１〜５０μｍであることを特徴とする請求項１または２に記載の陰イオン分析液体クロマトグラフィー用架橋重合体粒子。
【請求項４】 請求項１〜３のいずれかに記載の陰イオン分析液体クロマトグラフィー用架橋重合体粒子を用いた陰イオン分析液体クロマトグラフィー用充填剤。
【請求項５】 水酸基を持つアクリレート及びメタクリレート系架橋性モノマーからなる群より選ばれる１種または２種以上のモノマーを水性懸濁重合させ、得られた架橋ゲルの水酸基を利用してスペーサーを介し４級アンモニウム基を導入することを特徴とする請求項１〜３のいずれかに記載の陰イオン分析液体クロマトグラフィー用架橋重合体粒子の製造方法。
【請求項６】 請求項１〜３のいずれかに記載の陰イオン分析液体クロマトグラフィー用架橋重合体粒子を充填してなる陰イオン分析液体クロマトグラフィー用カラム。
[Claims]
1. Aqueous suspension polymerization of one or more monomers selected from the group consisting of acrylates having a hydroxyl group and methacrylate-based crosslinkable monomers, and via a spacer using the hydroxyl groups of the obtained crosslinked gel, via a spacer. Crosslinked polymer particles having an introduced quaternary ammonium group for anion analysis liquid chromatography.
(2) 2. The crosslinked polymer for anion analysis liquid chromatography according to claim 1, wherein the spacer is a residue of epichlorohydrin, ethylene glycol or 1,4-butanediol diglycidyl ether or ester, polyethylene glycol diglycidyl ether or ester. particle.
Claims310. The method according to claim 1, wherein the particle size is 1 to 50 .mu.m.Or 24. The crosslinked polymer particles for anion analysis liquid chromatography according to item 1.
Claims4] Any of claims 1 to 35. A filler for anion analysis liquid chromatography using the crosslinked polymer particles for anion analysis liquid chromatography according to item 1.
Claims5Aqueous suspension polymerization of one or more monomers selected from the group consisting of acrylates and methacrylate-based crosslinkable monomers having hydroxyl groups, and quaternary ammonium groups via spacers utilizing the hydroxyl groups of the obtained crosslinked gel. Characterized by introducingAny of claims 1 to 33. The method for producing crosslinked polymer particles for anion analysis liquid chromatography according to item 1.
Claims6] Any of claims 1 to 3A column for anion analysis liquid chromatography, which is packed with the crosslinked polymer particles for anion analysis liquid chromatography according to item 1.
【０００７】本発明において、水酸基を持つアクリレート及びメタクリレート系架橋性モノマーからなる群より選ばれる１種または２種以上のモノマーは、水性懸濁重合させ、架橋ゲルとする。水性懸濁重合は、水性媒体中で懸濁重合を行うものであるが、この水性媒体として水は必須であり、モノマーに対するその重量比は３００〜１０００重量％であり、より好ましくは４００〜８００重量％である。
【プルーフの要否】要
[0007] In the present invention, one or more monomers selected from the group consisting of acrylates and methacrylate cross-linking monomer having a hydroxyl group, it is an aqueous suspension polymerization, the crosslinked gel. Aqueous suspension polymerization is performs a suspension polymerization in an aqueous medium, this as an aqueous medium water is mandatory, the weight ratio of the monomers is 300 to 1000 wt%, more preferably 400 to 800 % By weight.
[Necessity of proof] Required
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP02248299A JP4341097B2 (en) | 1999-01-29 | 1999-01-29 | Crosslinked polymer particles for anion analysis liquid chromatography, production method thereof and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP02248299A JP4341097B2 (en) | 1999-01-29 | 1999-01-29 | Crosslinked polymer particles for anion analysis liquid chromatography, production method thereof and use thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2000221179A JP2000221179A (en) | 2000-08-11 |
| JP2000221179A5 true JP2000221179A5 (en) | 2006-02-02 |
| JP4341097B2 JP4341097B2 (en) | 2009-10-07 |
Family
ID=12083951
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP02248299A Expired - Lifetime JP4341097B2 (en) | 1999-01-29 | 1999-01-29 | Crosslinked polymer particles for anion analysis liquid chromatography, production method thereof and use thereof |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4341097B2 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4558187B2 (en) * | 2000-12-27 | 2010-10-06 | 昭和電工株式会社 | Anion exchanger, method for producing the same, column for ion chromatography, and method for measuring anion |
| US20040127648A1 (en) * | 2002-12-31 | 2004-07-01 | Ciphergen Biosystems, Inc. | Sorbent and method for the separation of plasmid DNA |
| TW200639190A (en) * | 2005-02-04 | 2006-11-16 | Showa Denko Kk | Packing material for ion chromatography |
| JP5399896B2 (en) * | 2006-06-26 | 2014-01-29 | ノバルティス アーゲー | Organic compounds |
| JP5749030B2 (en) * | 2011-02-15 | 2015-07-15 | 積水メディカル株式会社 | Method for producing column filler for liquid chromatography, method for measuring sample by liquid chromatography, and method for measuring hemoglobin |
| JP2012168054A (en) * | 2011-02-15 | 2012-09-06 | Sekisui Medical Co Ltd | Column packing material for liquid chromatography, method of manufacturing column packing material for liquid chromatography, and method of measuring hemoglobins |
| JP5749031B2 (en) * | 2011-02-15 | 2015-07-15 | 積水メディカル株式会社 | Method for producing column filler for liquid chromatography, method for measuring sample by liquid chromatography, and method for measuring hemoglobin |
-
1999
- 1999-01-29 JP JP02248299A patent/JP4341097B2/en not_active Expired - Lifetime
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