JP2000162504A - Enlarging observation device - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an enlarging observation device constituted so that an image being in focus can be obtained all over the area of an object to be observed and desired to be observed and the stereoscopic shape of the object to be observed can be easily grasped from the image being in focus. SOLUTION: This enlarging observation device is provided with a microscope lens barrel 7 obtaining the optical enlarged image of the object to be observed, an image pickup device 8 picking up the image of the optical enlarged image, the Z-axis feeding mechanism 11 of the lens barrel 7 being as the movement means changing a focusing position with respect to the object to be observed, a two-dimensional image data synthesis part 35 extracting a focusing point area being in focus from the plural pieces of image data picked up at different focus positions, synthesizing the respective focusing point areas and producing two-dimensional image data being in focus over the whole area, a picture display device 51 displaying the two-dimensional image data and a height information holding part 36 being as a stereoscopic information display means displaying the relative height information of the depth direction of a position designated by the two-dimensional image data displayed by the display device 51.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a magnifying observation apparatus for displaying, on an image display, an optically magnified image of an observation object obtained through an optical device such as a microscope.

[0002]

2. Description of the Related Art When a three-dimensional image of an object to be observed is enlarged and observed by an optical device such as a microscope, a part of the image may be blurred because the range of the depth of field is limited. is there. In this case, change the focal position,
By changing the magnification, focusing is performed, and the part to be observed is sharpened.

[0003]

However, in the prior art, particularly in optical equipment such as a microscope with a high magnification, the depth of field is extremely shallow, so that a sharp portion of the image is very small, and the object to be observed is very small. Cannot be observed at the same time. For example, in an observation target having a step such as a wiring pattern in a semiconductor device, a high portion and a low portion of the step may not be observed at the same time, so that the wiring pattern cannot be inspected quickly. For this reason, conventionally, there has been a demand for a technique for obtaining a focused image for all the regions to be observed of the observation target object irrespective of the depth of field of an optical device such as a microscope.

[0004] On the other hand, even if an image in which all of the region of the object to be observed is focused is obtained, this image is a two-dimensional image. In some cases, it is difficult to recognize the three-dimensional shape of the observation target from the two-dimensional image. For a technique for obtaining a three-dimensional image from a two-dimensional image of an observation target, see, for example, the article “Image processing and
synthesis for extended depth of opticalmicroscope
s, The Visual Computer (1992) 8: 351-360 ". In order to stereoscopically recognize the displayed image, for example, special glasses for the left and right eyes for stereoscopic viewing are used. It was not practical because of the need.

SUMMARY OF THE INVENTION The present invention has been made in view of the above-mentioned conventional problems, and can obtain an image in which all of a region to be observed of an object to be observed is in focus. It is an object of the present invention to provide a magnifying observation device capable of grasping a three-dimensional shape of an object.

[0006]

SUMMARY OF THE INVENTION The present invention provides an optical magnifying means for obtaining an optically magnified image of an object to be observed, an imaging means for taking the optically magnified image, and a focal position of the optical magnifying means with respect to the object to be observed. Moving means for changing the focal point, extracting a focused in-focus area from a plurality of image data captured at different focal positions, synthesizing each of the focused areas, and focusing on the two-dimensional area in all the areas. Two-dimensional image data synthesizing means for generating image data, an image display device for displaying the two-dimensional image data, and relative height information in a depth direction at an arbitrary position of the two-dimensional image displayed on the image display device Is displayed on the image display device.

[0007] The three-dimensional information display means holds relative height information in the depth direction of the two-dimensional image obtained from each pixel information of each of the focus areas, and is designated by the two-dimensional image. The height information of the position is output to the image display device.

[0008] The three-dimensional information display means continuously graph-displays the height information between any two points designated in the two-dimensional image.

There is provided instruction means for displaying a pointer on the screen of the image display device, setting a position of the pointer on the screen, and designating an arbitrary position of the two-dimensional image.

The moving means performs relative positioning of the observation object and the optical magnifying means in the depth direction of the two-dimensional image.

The optical magnification means is an optical microscope.

According to the present invention, the two-dimensional image data synthesizing means synthesizes a focused two-dimensional image in all regions, and this two-dimensional image is displayed on an image display device. Although it is difficult to directly grasp the three-dimensional shape of the observation target from the two-dimensional image, the relative height information in the depth direction at the designated position of the two-dimensional image is simultaneously displayed on the image display device by the three-dimensional information display means. Therefore, the three-dimensional shape of the observation target can be easily grasped from the height information.

[0013]

Embodiments of the present invention will be described below with reference to the drawings. FIG. 1 is an explanatory diagram illustrating a configuration of a microscope magnification observation system according to an embodiment of the present invention. In FIG. 1, a microscope magnification observation system according to the present embodiment includes an imaging unit 1 and an image processing / drive control device 3.
1 and an image display device 51. The imaging unit 1 includes an XY table 3 provided on a base 2, a microscope barrel 7 provided on a column 9 erected on the base 2, an imaging device 8 attached to the microscope barrel 7, It has an objective lens 6 attached to a microscope lens barrel 7 and an illumination device 10 provided in a column 9.

The XY table 3 is movably held in the X-axis and Y-axis directions on the base 2 by a slider (not shown), and the observation object W is placed on the XY table 3. The XY table 3 is provided with an X-axis feed mechanism 4 for moving and positioning the XY table 3 along the X-axis direction. The X-axis feed mechanism 4 is composed of, for example, an electric motor and a feed screw. It is positioned at an arbitrary position in the X-axis direction. XY table 3
Is provided with a Y-axis feed mechanism 5 for moving and positioning the XY table 3 along the Y-axis direction. Like the X-axis feed mechanism 4, the Y-axis feed mechanism 5 includes, for example, an electric motor and a feed screw.
The XY table 3 is positioned at an arbitrary position in the Y-axis direction by screwing the feed screw with an electric motor.

The microscope lens barrel 7 is for obtaining an optically enlarged image of the observation object W, and is composed of, for example, a zoom-type combination lens. The zoom lens is driven by a zoom lens driving unit (not shown) including an electric motor and the like built in the microscope lens barrel 7. Objective lens 6
Is provided at the tip of the microscope lens barrel 7 for optically enlarging the observation target W. The microscope lens barrel 7 has a column 9
Movably provided in the column 9,
For example, it is positioned at an arbitrary position in the Z-axis direction by a Z-axis feed mechanism 11 composed of an electric motor and a feed screw.

The imaging device 8 is, for example, a CCD (charge
coupled device) is a color camera using an image pickup device, is provided at the upper end of the microscope tube 7, and is an observation object W enlarged through the objective lens 6 and the microscope tube 7.
The optical magnified image of is taken.

The illumination device 10 is mounted on the microscope lens barrel 8 to illuminate the observation object W with light. Light emitted from the illumination device 10 illuminates the observation target W from above through the microscope lens barrel 8 and the objective lens 6.

The image processing / drive control device 31 drives a zoom lens drive unit provided in the microscope barrel 7 so that the optical magnification of the microscope barrel 7 becomes a designated value. Performs lens positioning control. Also,
The image processing / drive control device 31 captures the image data captured by the image capturing device 8 described above, performs a predetermined process on the image data, and displays the image data on the image display device 51.

Further, a mouse 40 is connected to the image processing / drive control device 31. The image processing / drive control device 31 sets the position of the pointer 38 a displayed on the screen 52 of the image display device 51 based on a signal input by operating the mouse 40,
Various processes are performed by a signal based on the operation of the two operation buttons 40a and 40b provided in “0”.

The image processing / drive control device 31 is, for example, a personal computer (hereinafter referred to as a personal computer).
Then, the electric motors of the image processing board and the zoom lens driving unit provided in the microscope barrel 7 and the electric motors of the X-axis feed mechanism 4, the Y-axis feed mechanism 5, and the Z-axis feed mechanism 11 are controlled. It can be configured by adding a motor control board and providing software for operating these boards in a personal computer. As the image display device 51, for example, a monitor for a personal computer can be used.

FIG. 2 is an explanatory diagram showing an example of the configuration of the image processing / drive control device 31. Image processing / drive control device 3
1 is a movement control unit 32, an image data input unit 33, an image data storage unit 34, a two-dimensional image data synthesis unit 35
A height information holding unit 36, a mouse signal control unit 37,
An image display unit 38 and a zoom lens drive control unit 39 are provided.

The movement control unit 32 sends a control signal 32s for controlling the rotational position of each electric motor of the X-axis feed mechanism 4, the Y-axis feed mechanism 5 and the Z-axis feed mechanism 11 to the X-axis feed mechanism 4, the Y-axis feed mechanism 4, and the Y-axis feed mechanism 4. Output is performed to the feed mechanism 5 and the Z-axis feed mechanism 11, respectively, to perform positioning control for moving the observation target W and the microscope lens barrel 7 to target positions. Z-axis feed mechanism 1
1 to drive the observation object W of the microscope lens barrel 7.
Can be changed arbitrarily. In addition, the movement control unit 32 can control the driving of the X-axis feed mechanism 4, the Y-axis feed mechanism 5, and the Z-axis feed mechanism 11 according to a control signal 37s1 from the mouse signal control unit 37 based on the operation of the mouse 40. Has become.

The zoom lens drive control unit 39 receives a signal 37s2 input from the mouse signal control unit 37,
An enlargement ratio designation signal 39 s for controlling the drive of the electric motor of the zoom lens drive unit built in the microscope lens barrel 7 is output to the zoom lens drive unit. Thereby, the position of the zoom lens is controlled such that the magnification of the microscope lens barrel 7 becomes the designated magnification.

The image data input unit 33 takes in the image data of the optically enlarged image of the observation object W by the microscope barrel 7 by the image pickup device 8 into the image processing / drive control device 31. The image data storage unit 34 stores and holds a plurality of image data captured from the image data input unit 33.

The mouse 40 connected to the mouse signal controller 37 detects the amount and direction of rotation of a roller provided below the mouse 40 by moving the mouse 40 on a plane, for example. The corresponding signal 40s is output. In addition, the mouse 40 has left and right operation buttons 40.
a and 40b, and these operation buttons 40a
By operating (clicking on) and 40b, a signal 40s is output to the mouse signal control unit 37.

The mouse signal control unit 37 outputs a signal 4 by moving the mouse 40 and operating the operation buttons 40a and 40b.
0s, X-axis feed mechanism 4, Y-axis feed mechanism 5
And a control signal 37s1 for the Z-axis feed mechanism 11 is appropriately generated. For example, the mouse pointer 38 a is moved to an operation switch for positioning the X-axis feed mechanism 4, the Y-axis feed mechanism 5, and the Z-axis feed mechanism 11 displayed at a predetermined position on the screen 52 of the image display device 51. Operation button 4
By clicking on 0a or 40b, the control signal 37s1 can be generated. With such a configuration, a desired position of the observation target W can be imaged as a desired focal position by operating the mouse 40.

The mouse signal control unit 37 holds the coordinate position of the mouse pointer 38a on the screen 52 of the image display device 51, and sets the position of the mouse pointer 38a based on the signal 40s from the mouse 40. To generate a mouse position setting signal 37s3 for output to the image display unit 38, and based on a signal 40s from the mouse 40,
An enlargement ratio designation signal 37s2 output to the zoom lens drive controller 39 is generated.

Further, the mouse signal control unit 39 controls the mouse 4
Based on the signal 40s from 0, a position designation signal 37s4 for designating the position of the two-dimensional image displayed on the screen 52 of the image display device 51 is output to the height information holding unit 36. For example, the mouse pointer 38a is moved to the image display device 5
By positioning the operation button 40a at a desired position on the first screen 52 and double-clicking the operation button 40a at this position, 2
A configuration for generating a position designation signal 37s4 for designating the position of the two-dimensional image may be employed. The various processing contents according to the operation contents of the mouse 40 of the mouse signal control unit 39 can be arbitrarily set by changing software.

The two-dimensional image data synthesizing section 35 extracts a focused in-focus area from a plurality of pieces of image data having different focal positions stored in the image data storage section 34 and synthesizes each of these in-focus areas. By doing so, two-dimensional image data focused on all areas is generated.

The height information holding unit 36 stores the relative height data Hd in the depth direction of each pixel of the two-dimensional image data focused on all the regions generated by the two-dimensional image data synthesizing unit 35. Acquired from the two-dimensional image data synthesis unit 35,
I hold this. Further, the height information holding unit 36 is provided with a position designation signal 37s input from the mouse signal control unit 37.
According to 4, height information 36 s of the designated coordinate position of the two-dimensional image data focused on all the areas is output to the image display unit 38.

The image display section 38 displays the two-dimensional image data 35 s output from the two-dimensional image data synthesizing section 35 on the screen 52 of the image display device 51. Also, the height information 36s output from the height information holding unit 36 is displayed on the screen 52 of the image display device 51 in a predetermined format. Further, in accordance with the mouse position setting signal 37s3 from the mouse signal control 37, the mouse pointer 3 on the screen 52 of the image display device 51 is displayed.
The display position of 8a is updated. Furthermore, various screen displays such as various operation switches and windows are performed. The image display unit 38 can be composed of, for example, graphic software or a graphic circuit for performing image processing.

Here, the principle of synthesizing the two-dimensional image data focused on all the regions of the two-dimensional image data synthesizing section 35 will be described. For example, as shown in FIG. 3, a case will be described in which a quadrangular pyramid-shaped observation target object W is placed on the XY table 3 and observed.
In FIG. 3, the Z-axis direction position of the microscope lens barrel 7 provided with the objective lens 6 at the tip with respect to the observation target W is denoted by PA and P.
B and PC, the focal position of the microscope lens barrel 7 is changed, and the observation object W placed on the XY table 3 is changed.
The optical magnified image of is taken.

FIG. 4 shows an example of image pickup data of an optically enlarged image of the observation object W at each focal position. Microscope tube 7
As shown in FIG. 4 (a), the image data G obtained by moving to the axial position PA and capturing an optically enlarged image of the observation object W includes, as shown in FIG. Out of focus region R2. Focusing area R1
Is the mounting surface of the XY table 3 and the observation object W in the form of a pyramid.
Consists of the lower region of the conical surface of.

Image data G obtained by moving the microscope lens barrel 7 to a position PB in the Z-axis direction and capturing an optically enlarged image of the observation object W
As shown in FIG. 4 (b), only the region corresponding to the intermediate region of the pyramidal surface of the quadrangular pyramid-shaped observation object W has the focal region R
4 and the areas corresponding to the lower area and the top area of the mounting surface of the XY table 3 and the pyramidal surface of the quadrangular pyramid-shaped observation object W are out-of-focus areas R3 and R5.

Image data G obtained by moving the microscope lens barrel 7 to the position PC in the Z-axis direction and capturing an optically enlarged image of the observation object W
As shown in FIG. 4C, only the region corresponding to the intermediate region of the pyramidal surface of the quadrangular pyramid-shaped observation target W is focused on the focal point region R.
4 and the areas corresponding to the lower area and the top area of the mounting surface of the XY table 3 and the pyramidal surface of the quadrangular pyramid-shaped observation object W are out-of-focus areas R3 and R5.

As described above, the microscope barrel 7 of the observation object W is
Only the area corresponding to the depth of field of the image data G becomes the focused area. By combining the focused areas shown in FIGS. 4A to 4C, all the areas of the image data G are focused. Two-dimensional image data can be obtained. It should be noted that, as the magnification of the microscope lens barrel 7 increases, the depth of field becomes shallower and the focused area becomes narrower. Therefore, a large number of image data having different focal positions are required. The required number of image data is appropriately determined from the magnification of the microscope lens barrel 7 and the like.

A method of extracting a focused area from each image data is described in, for example, the article “Image processing and synthesis”.
for extended depth of optical microscopes, The Vi
sualComputer (1992) 8: 351-360 ". As described in the above paper,
In general, in image data of an optically magnified image obtained by a microscope, a region in focus contains a higher spatial frequency, and a region out of focus has a lower spatial frequency. , The most focused region can be extracted. That is, at the same X and Y coordinates of a plurality of image data, by comparing the degree of focus from the difference in spatial frequency, the most in-focus area is extracted from the image data at a plurality of different focus positions. Can be.

Further, as described in the above-mentioned paper, each pixel of the two-dimensional image data focused on all regions has a relative height h as shown in FIG.
Information. The information of the height h corresponds to the degree of focus of each pixel of the focused two-dimensional image data. Therefore, the above-described height information holding unit 36
Can obtain height information as shown in FIG. 5,
The relative height information (the relative coordinate value in the Z direction) of each pixel of the focused two-dimensional image data in the depth direction can be held.

FIG. 6 is an explanatory diagram showing a display example of the image display device 51 of the microscope magnification observation system having the above configuration. As shown in FIG. 6, on a screen 52 of the image display device 51,
An observation target W, for example, a two-dimensional image of a circuit board, captured by the imaging device 8 through the microscope lens barrel 7 is optically enlarged at a predetermined magnification and is displayed. The two-dimensional image displayed on the screen 52 is an image in which all areas are in focus.

A mouse pointer 38a is displayed on the screen 52 of the image display device 51.
The coordinate display section 53 on the right side of the screen 52 displays the relative position of the position P of the two-dimensional image pointed by the mouse pointer 38a from the reference position in the X, Y, and Z directions. For example,
When the mouse pointer 38a is moved to an arbitrary position and the operation button 40a of the mouse 40 is clicked at that position,
Height information of the two-dimensional image in the Z direction can be displayed. Since the height information in the Z direction, which is the depth direction of the two-dimensional image, is displayed, the mouse 40 is operated to move the mouse pointer 38a to an arbitrary position in the two-dimensional image, so that the Z direction at an arbitrary position in the Z direction is displayed. Height information can be easily grasped. Although FIG. 6 shows the height information of the position P in the Z direction, it is also possible to specify a plurality of points and display the height information of the plurality of points in the Z direction at the same time.

FIGS. 7 and 8 are diagrams for explaining other display examples of the image display device 51 of the microscope magnification observation system having the above configuration. FIG. 7 shows a state where two arbitrary points P1 and P2 of the two-dimensional image displayed on the screen 52 are designated by operating the mouse 40. When any two points P1 and P2 of the two-dimensional image are designated by operating the mouse 40,
The XY coordinate information of these two points P1 and P2 is input to the height information holding unit 36 through the mouse signal control unit 37 of the image processing / drive control device 31. The height information holding unit 36 outputs the height information of the two-dimensional image of the line segment connecting the two points P1 and P2 to the image display unit 38 based on the input XY coordinate information of the two points P1 and P2.

The image display unit 38 displays the height information of the two-dimensional image between the lines connecting the two points P1 and P2 in a new display window 55, for example, as shown in FIG.
In the display window 55, the height information of the line segment connecting the two points P1 and P2 is continuously displayed in a graph. Accordingly, the surface shape of the observation target W can be grasped, and the three-dimensional shape of the observation target W which is difficult to visually grasp only with the height information of each position of the two-dimensional image can be easily grasped. it can.

As described above, according to the present embodiment, while viewing the two-dimensional image of the observation object W focused on all the areas displayed on the screen 52 of the image display device 51, the depth of an arbitrary position is determined. The length measurement in the vertical direction is possible, and the three-dimensional shape of the observation target W can be grasped from the two-dimensional image. That is, it is possible to observe a large area of a fine observation object at a high depth of field that cannot be seen only with an optical device such as a microscope, and at the same time, only with an optical device such as a microscope while viewing a two-dimensional image of the observation object. This makes it difficult to measure the length of the observation target in the depth direction. Further, since the position of the two-dimensional image can be specified by operating the mouse 40, the operation is very simple. Further, since the two-dimensional image displayed on the screen 52 of the image display device 51 is in focus in all areas, it is very easy to specify a position at which height information in the Z direction in the two-dimensional image is to be checked. Become. In addition, the image display device 51
By designating arbitrary two points P1 and P2 of the two-dimensional image on the screen 52 of FIG. 2 and continuously displaying the height information of the line segment connecting the two points P1 and P2 in a graph, the object to be observed from the two-dimensional image is displayed. W
It becomes easier to grasp the three-dimensional shape.

In the above-described embodiment, the position information in the depth direction (Z-axis direction) of the observation target W is obtained from the image data obtained through the microscope lens barrel 7. However, the present invention is not limited to this. Do not mean. For example, a laser-type distance-measuring sensor is provided for the XY table 3, and the laser-type distance-measuring-type sensor is scanned in the X-axis and Y-axis directions so that the height of the observation target W in the Z-axis direction is increased. It is also possible to adopt a configuration in which the height information is obtained and held in the height information holding unit 36 of the image processing / drive control device 31. In this case, Z is obtained from image data obtained at a plurality of different focal positions.
Height information with higher accuracy can be obtained than when height information in the axial direction is obtained.

[0045]

According to the present invention, it is possible to measure the length of an observation object in the depth direction while observing a focused two-dimensional image in all regions. As a result, the three-dimensional shape of the observation target can be grasped from the focused two-dimensional image. Further, according to the present invention, an enlarged image of the observation target is displayed on the image display device, so that a plurality of observers can observe the observation target at the same time.

[Brief description of the drawings]

FIG. 1 is an explanatory diagram showing a configuration of a microscope magnification observation system according to an embodiment of the present invention.

FIG. 2 is an explanatory diagram illustrating a configuration example of an image processing / drive control device according to the embodiment;

FIG. 3 is a diagram for explaining the principle of synthesizing two-dimensional image data in focus in all regions.

FIG. 4 is an example of imaging data of an optically enlarged image of an observation target W at each focal position.

FIG. 5 is a diagram for explaining height information in the depth direction obtained from each pixel of the two-dimensional image data in focus in all regions.

FIG. 6 is a diagram for explaining a display example of an image display device of the microscope magnification observation system according to one embodiment of the present invention.

FIG. 7 is a diagram for explaining another display example of the image display device of the microscope magnifying observation system according to one embodiment of the present invention.

FIG. 8 is a diagram for explaining a display example following the display shown in FIG. 7;

[Explanation of symbols]

DESCRIPTION OF SYMBOLS 1 ... Image pick-up part, 3 ... XY table, 4 ... X-axis feed mechanism part,
5 Y-axis feed mechanism 6 Objective lens 7 Microscope barrel
8 imaging device 31 image processing / drive control device 32
Movement control unit, 33 image data input unit, 34 image data storage unit, 35 two-dimensional image data synthesis unit, 36 height information storage unit, 37 mouse signal control unit, 38 image display unit, 39 ... Zoom lens drive controller, 40 ... mouse, 5
1. Image display device.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI Theme coat ゛ (Reference) H04N 5/232 H04N 7/18 U 7/18 C G06F 15/62 350A (72) Inventor Hideo Nakamura Tokyo 6-7-35 Kita-Shinagawa, Shinagawa-ku Sony Corporation (72) Inventor Toru Kumagai 6-7-35 Kita-Shinagawa, Shinagawa-ku, Tokyo Sony Corporation F-term (reference) 2H052 AF17 AF21 AF25 5B050 BA09 BA13 DA01 EA04 EA19 FA02 FA09 5C022 AA01 AB21 AB65 AB66 AB68 AC01 AC13 AC14 AC31 AC51 AC69 AC74 5C054 CC05 CD03 CF03 CF07 EH01 FC12 FE16 FE19 HA05 5C076 AA19

Claims (6)

[Claims] 1. An optical magnifying means for obtaining an optically magnified image of an observation object, an imaging means for photographing the optical magnified image, and a moving means for changing a focal position of the optical magnifying means with respect to the observation object. A two-dimensional image in which in-focus areas are extracted from a plurality of image data captured at the focal position, and these in-focus areas are combined to generate two-dimensional image data in which all areas are in focus Data combining means; an image display device for displaying the two-dimensional image data; and relative height information in a depth direction at a designated position of the two-dimensional image displayed on the image display device is displayed on the image display device. Magnifying observation device having a three-dimensional information display means for performing the operation. 2. The stereoscopic information display means holds relative height information in a depth direction of the two-dimensional image obtained from each pixel information of each of the focus areas. The magnifying observation apparatus according to claim 1, wherein height information of a designated position is output to the image display device. 3. The magnifying observation apparatus according to claim 1, wherein the three-dimensional information display means continuously displays the height information between any two points designated by the two-dimensional image in a graph. 4. The apparatus according to claim 1, further comprising an instruction unit for displaying a pointer on a screen of the image display device, setting a position of the pointer on the screen, and specifying an arbitrary position of the two-dimensional image. Magnifying observation device. 5. The magnifying observation apparatus according to claim 1, wherein the moving means performs relative positioning of the observation object and the optical magnifying means in a depth direction of the two-dimensional image. 6. The magnifying observation apparatus according to claim 1, wherein said optical magnifying means is an optical microscope.