JP2000159605A - Bactericidal agent - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a low-cost bactericidal agent excellent in bactericidal activity, having good contacting properties with a bactericidal object, capable of performing a bactericidal treatment in a short time and not giving effects on quality of food, etc. SOLUTION: This bactericidal agent comprises at least one substance selected from organic acids or their salts, at least one substance selected from inorganic acids or their salts and a bactericidal agent containing saponin. Lactic acid as the organic acid, phosphoric acid as the inorganic acid, and a saponin originated from quillaja as the saponin are preferably used, respectively. The bactericidal agent exhibits an excellent bactericidal effect in a short time, by preparing the agent to contain the lactic acid in an amount of 5-91 wt.%, the phosphoric acid in an amount of 1.5-86 wt.% and the saponin in an amount of 0.05-6.0 wt.%, and to have a controlled pH of <=1.2, diluting the prepared agent with water by 100-200 times, and then contracting the diluted solution with an object of the bactericidal treatment.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a disinfectant for disinfecting bacteria adhered to foods, food raw materials, food processing equipment and the like.

[0002]

2. Description of the Related Art In recent years, food poisoning due to bacteria attached to foods and the like and spoilage of foods have become serious problems. Examples of the contamination route by the bacteria include contamination of food raw materials and contamination in food processing and distribution processes.

[0003] Among the above-mentioned food raw materials, for example, it is difficult to remove bacteria as a contaminant from fresh food vegetables, fresh fish and shellfish, eggs and the like that are not subjected to heat treatment only by washing with water. On the other hand, for example, processed foods such as kamaboko are said to be higher in safety than the non-heated foods because heat treatment is performed in the processing step. However, these processed foods may be subjected to secondary infection from various devices or the human body before being packaged after the heat treatment.

[0004] In order to avoid such contamination by bacteria, various studies have been made on the sterilization, bacteriostasis, and bacteriostasis of the bacteria. For example, chemicals such as sodium hypochlorite are used. Sterilization methods have been implemented.

[0005] However, when the above-mentioned sodium hypochlorite or the like is used, it is necessary to use the drug at a high concentration in order to obtain a sufficient bactericidal effect. Flavor is impaired. on the other hand,
If the drug is used at a low concentration, the bactericidal effect is reduced, so that, for example, food must be brought into contact with the drug for a long time. For this reason, there is a possibility that the food may be affected by the chemicals such as denaturation.

In addition, as a bactericide, for example, an organic acid such as acetic acid or lactic acid or an inorganic acid such as phosphoric acid is used. In this case, too,
The flavor of the food is impaired.

To solve such a problem, Japanese Patent Publication No. Sho 6
Japanese Patent Application Publication No. 2-28664 discloses a bactericide comprising ethanol, an organic acid and an inorganic acid. According to this disinfectant, the concentration of each of the components can be reduced, and disinfection can be performed without remarkably reducing the flavor and quality of the food. However, with this disinfectant, for example, when the water repellency of the surface of the food is high, the contact with the food may be poor, and the disinfecting effect may be low. Further, since ethanol is used, there is a problem of remaining in foods and a problem of increasing costs.

[0008]

Therefore, the present invention has excellent sterilizing power and good contact with an object to be sterilized.
An object of the present invention is to provide a low-cost disinfectant that can perform a disinfection treatment in a short time and does not affect the quality of foods and the like.

[0009]

In order to achieve the above object, the bactericide of the present invention comprises at least one substance selected from organic acids and salts thereof and at least one substance selected from inorganic acids and salts thereof. And saponin.

According to the bactericide of the present invention, since the saponin is contained as a surfactant, even if the surface of the object to be sterilized is water-repellent, the treatment time can be short, as described above. Even if the amount of the bactericide is small, an excellent bactericidal effect is exhibited. Further, since the organic acid and the inorganic acid are contained, dissociation (ionization) of the organic acid due to a change in pH can be prevented. That is, for example, the bactericidal effect of an organic acid depends on the fact that the organic acid is a non-dissociated type, so that when the dissociation is prevented, the bactericidal effect is improved as compared with the case where the organic acid is used alone. Can be. Therefore, it is possible to exhibit an excellent bactericidal effect at a significantly lower concentration than when using a high-concentration organic acid or an inorganic acid alone, and to achieve cost reduction. The same effect can be obtained by using the salt of the organic acid and the salt of the inorganic acid.

[0011] In the fungicide of the present invention, the content of at least one substance selected from the above-mentioned organic acids and salts thereof is in the range of 0.05 to 91% by weight, and at least one selected from the above-mentioned inorganic acids and salts thereof. The content of one substance is preferably in the range of 0.015 to 86% by weight, and the content of the saponin is preferably in the range of 0.0005 to 6.0% by weight. This fungicide is prepared from the beginning to the concentration at the time of use, and used as it is (hereinafter, referred to as
It may be a "straight type", or may be a type that is initially prepared as a concentrated liquid and used after dilution with water or the like (hereinafter, referred to as a "concentrated type"). The contents of these components are not particularly limited, and are appropriately determined depending on, for example, the type of the object to be sterilized, the degree of contamination by bacteria, the sterilization time, the treatment method, and the like.

In the fungicide of the present invention, the concentration of each of the above components during use is not particularly limited. For example, the content of at least one substance selected from the above-mentioned organic acids and salts thereof is 0.05 to 1%. The content of at least one substance selected from the inorganic acids and salts thereof is 0.015 to 1.7% by weight, more preferably in the range of 0.8% by weight, more preferably in the range of 0.1 to 0.7% by weight. , More preferably in the range of 0.015 to 0.5% by weight, and the saponin content is in the range of 0.0005 to 0.06% by weight, more preferably 0.002 to 0.02% by weight. % Is preferable.

In the bactericide of the present invention, in the case of the concentrated type, the content of at least one substance selected from the organic acids and salts thereof is in the range of 5 to 91% by weight,
More preferably, the content of at least one substance selected from the inorganic acids and salts thereof is in the range of 10 to 70% by weight, and the range of 1.5 to 86% by weight is more preferable.
In the range of 1.5 to 50% by weight, the content of the saponin is:
In the range of 0.05 to 6.0% by weight, more preferably, 0.1 to 6.0% by weight.
It is preferably in the range of 15 to 3.0% by weight. The dilution ratio when using the concentrated-type bactericide is not particularly limited, and is appropriately determined by, for example, the type of the bactericidal treatment target, the degree of contamination by bacteria, the bactericidal treatment time, the treatment method, and the like. , 50 to 300 times dilution, preferably 100 to 200 times dilution. In addition, the concentrated type bactericide may be used without dilution, for example, depending on the type of the object to be sterilized. In addition, the content of each of the components in the concentrated type bactericide is not particularly limited.

As a result of diligent research, the present inventors have found that saponin is the most excellent surfactant as a fungicide of the present invention. For example, when preparing a bactericide containing an organic acid, an inorganic acid and a surfactant, among various surfactants, saponins are particularly excellent in solubility, and surfactants other than the saponins are, for example, separated. Liable to float on the surface of the solution and not be dissolved. Especially when the concentrated type fungicide is set to an effective component composition,
Saponin is excellent in its solubility. Therefore, if saponin is used as a surfactant, it is possible to prepare a disinfectant in which each component is uniformly dissolved, whether it is a straight type or a concentrated type, and dilutes the concentrated type disinfectant. Even when used, it shows a sufficient bactericidal effect.

In the bactericide of the present invention, it is preferable that the saponin is a saponin derived from Quillaja. The saponin is not limited to the saponin derived from Kiraya, and other saponins such as those derived from yucca, soybean, beet, fia, tea seed, and endju can be used. In addition, the saponin may be used alone or in combination of two or more.

In the bactericide of the present invention, the organic acid is
Lactic acid, acetic acid, malic acid, citric acid, tartaric acid, ascorbic acid, gluconic acid, fumaric acid, and at least one organic acid selected from the group consisting of adipic acid, wherein the salt thereof is lactic acid, acetic acid, malic acid, citric acid. Preferably, the salt is at least one organic acid selected from the group consisting of acids, tartaric acid, ascorbic acid, gluconic acid, fumaric acid and adipic acid. The organic acid and its salt may be used alone or in combination of two or more.

In the bactericide of the present invention, the inorganic acid is
Phosphoric acid, pyrophosphoric acid, polyphosphoric acid, at least one inorganic acid selected from the group consisting of sulfuric acid and hydrochloric acid,
The salt is preferably a salt of at least one inorganic acid selected from the group consisting of phosphoric acid, pyrophosphoric acid, polyphosphoric acid, sulfuric acid and hydrochloric acid. The inorganic acid and its salt may be used alone or in combination of two or more.

In the fungicide of the present invention, it is preferable that the organic acid is lactic acid and the inorganic acid is phosphoric acid.

In the bactericide of the present invention, the pH is preferably not more than 3.0, particularly preferably not more than 2.5, because dissociation of the organic acid can be further prevented.
It is as follows. When the bactericide is of a concentrated type, its pH can be appropriately determined, for example, based on the content of each of the components, the dilution ratio, and the like. As described above, the pH is preferably 3.0 or less, more preferably 2. 5 or less, particularly preferably pH 1.2 or less.

[0020]

BEST MODE FOR CARRYING OUT THE INVENTION The fungicide of the present invention can be prepared by, for example, dissolving lactic acid, phosphoric acid and saponin in water.

The bactericide of the present invention may contain, for example, sodium hydroxide, hydrochloric acid, sulfuric acid, etc., in addition to the above components, for adjusting pH. Further, other components may be contained as long as they do not hinder the fungicide of the present invention.

Further, the bactericide of the present invention, as described above,
A straight-type disinfectant that is set to the component concentration at the time of use may be used, but, for example, a concentration-type disinfectant is preferable because the concentration can be appropriately determined depending on the degree of bacterial contamination or the type of the object to be disinfected. . Also,
If it is a concentrated type, the volume can be reduced even when a large amount is required, and it can be diluted and used according to the required amount at the time of use, so it is particularly used in a food factory or the like that uses a large amount of a bactericide. Very convenient in cases etc.

When diluting the concentrated type bactericide,
The solvent is usually water, but the solvent is not particularly limited as long as the disinfectant is not hindered.

Next, as a method of sterilization using the germicide of the present invention, for example, there is a method of bringing the germicide into contact with an object to be sterilized.

Examples of the contact method include immersion, spraying, wiping and the like. The method is not particularly limited, but is usually determined appropriately depending on the type of the object to be sterilized and the state of contamination.

The object to be sterilized is not particularly limited, and examples thereof include food, food raw materials, food containers, food processing equipment, and the like.

Examples of the food and food ingredients include processed foods of meat and seafood such as vegetables, fish and shellfish, meat, eggs, kamaboko, starch, sausage, wiener, ham, bacon and the like.

As the food utensil, for example, tableware,
Examples include cutting boards, kitchen knives, cloths, chopsticks, food storage containers, and the like.

Examples of the food processing equipment include a stirrer, a homogenizer, a pressing machine, a filter, and an automatic cutter.

The object of the sterilization is not limited to the foods and the like, and may include, for example, fingers of cooks and workers involved in distribution and processing steps, fingers of general consumers, and the like. Sterilization can be performed by impregnating with the above-mentioned disinfectant or wiping it off with gauze or the like containing the same to prevent contamination of foods and the like.

The sterilization treatment time of the present invention is appropriately determined depending on the type of the object to be sterilized, the contact method, the concentration of each component of the disinfectant, and the like, and is usually 0.5 to 30 minutes. The germicide of the present invention exhibits a bactericidal effect even if the treatment time is within 0.5 minutes, and does not affect the quality of foods and the like even if the treatment time is 30 minutes or more.

The bacteria which can be sterilized by the bactericide of the present invention are not particularly limited, and include, for example, general Escherichia coli (Escherichia coli) and pathogenic Escherichia coli O157 (Escherichia coli cellotype O157: Es).
cherichia coli SEROTYPE O157), Staphylococcus aureus: Staphyrococcus
aureus), Salmonella typhimurium (Salmonella thyphimurium, Salmonella enteritidis), Vibrio parahaemolyticus (Vibrio para
haemolyticus), Bacillus cereus: Ba
cillus cereus).

[0033]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of the present invention will be described below.

(Example 1) The bactericidal effect of the present bactericide on general Escherichia coli was examined. First, the composition of the bactericide and the culture medium used are shown below.

(Bactericide: pH 2.17) D, L-lactic acid (manufactured by Showa Kako Co., Ltd .: same hereafter) 0.35% by weight Phosphoric acid (manufactured by Nippon Chemical Industry Co., Ltd .: same hereafter) 0.12% by weight sodium hydroxide (Tosoh Corporation: the same applies hereinafter) 0.012% by weight Kiraya extract: Saponin 0.0025% by weight (Natural Response SA: the same applies hereinafter) Water residue

(Strain used: general Escherichia coli) Escherichia coli IFO 3301

(Normal broth medium: Eiken Chemical Co., Ltd.) Meat extract 0.3% by weight Peptone 1.0% by weight Sodium chloride 0.5% by weight Water residue Adjusted to pH 7.0

(Desoxycholate agar medium for detection:
Peptone 1.0% by weight Iron ammonium citrate 0.2% by weight Sodium chloride 0.5% by weight Potassium hydrogen phosphate 0.2% by weight Lactose 1.0% by weight Neutral red 0.0033% by weight Agar 1.5% by weight Deionized water Residue Adjusted to pH 7.0

(Physiological saline) Sodium chloride (manufactured by Katayama Chemical Co., Ltd.) was dissolved in ion-exchanged water to a concentration of 0.9% by weight, and the pH was adjusted to 7.0.

(Sterilization conditions) A 5 ml liquid medium was added to a test tube.
After each dispensing, the test tube was capped with a silicon stopper and steam sterilized at 120 ° C. for 20 minutes in an autoclave at 1 atm. In addition, the agar medium is 8m in a test tube.
After dispensing 1 l each, the test tube was capped with a silicone stopper and steam sterilized in the same manner as in the liquid medium. After sterilization, the temperature of the agar medium was kept at 50 ° C. until use so that the agar did not solidify.

(Operation method) One loopful of the Escherichia coli was inoculated into 5 ml of the sterilized ordinary broth medium, and cultured at 35 ° C for about 20 hours with shaking. This culture solution was diluted 100-fold with sterilized physiological saline, and this was used as a sample solution. To 0.05 ml of this sample solution, 4.95 ml of the bactericide was added and mixed, and each treatment time (1 minute, 2 minutes)
Minutes, 3 minutes, 5 minutes and 10 minutes). 0.05 ml of this treated sample solution was added to 50 mM phosphate buffer 4.95.
ml (pH 7.0) and mixed.
It was added to a sterilized petri dish. Next, 8 ml of the sterilized desoxycholate agar medium for detection kept at 50 ° C. was poured into the petri dish and mixed well with the sample mixture. After the agar medium was solidified, the dish was covered and incubated at 37 ° C. for 20 hours. Then, the number of colonies of Escherichia coli grown on the desoxycholate agar medium was counted.

As a control, the operation was performed in the same manner as described above, except that a sterile physiological saline was mixed with the sample solution instead of the bactericide.

(Evaluation method of bactericidal effect) Using the number of colonies of Example 1 (the number of colonies of the treated sample) and the number of colonies of the control (the number of colonies of the untreated sample), the killing rate of the bacterial cells was calculated by the following formula. I asked. The bactericidal effect of the bactericide can be evaluated based on the kill rate. The results are shown in Table 1 below.

[0044]

[Equation 1] Death rate (%) = [(B−A) / B] × 100 A: Number of colonies of treated sample B: Number of colonies of untreated sample

Example 2 The bactericidal effect of the present bactericide on pathogenic Escherichia coli was examined. The same germicide and medium as used in Example 1 were used.

(Strain used: pathogenic Escherichia coli O157) Escherichia coli cellotype O157: H7 ATCC
43888 (Escherichia coli SEROTYPE O157: H7 ATCC 4388
8)

(Operation method) The bactericidal effect was evaluated in the same manner as in Example 1 except that the pathogenic Escherichia coli O157 was used instead of the Escherichia coli. The results are shown in Table 1 below.
Shown in

Example 3 The fungicidal effect of the fungicide of the present invention on two kinds of Salmonella was examined. The composition of the medium used is shown below. The same fungicide as in Example 1 was used.

(Strain used: Salmonella) Salmonella thyphimurium IFO 14194 Salmonella enteritidis

(Ordinary agar medium for detection: Eiken Chemical Co., Ltd.) Meat extract 0.5% by weight Peptone 1.0% by weight Sodium chloride 0.5% by weight Agar 1.5% by weight Water residue Adjusted to pH 7.0

(Operation method) The same procedure as in Example 1 was carried out except that each of the above-mentioned Salmonella bacteria was used in place of the above-mentioned Escherichia coli and the above-mentioned ordinary agar medium for detection was used in place of the above desoxycholate agar medium for detection. Then, the bactericidal effect was evaluated. The results are shown in Table 1 below.

(Example 4) The bactericidal effect of the present bactericide on Staphylococcus aureus was examined. The same fungicide and medium as used in Example 3 were used.

(Strain used: Staphylococcus aureus) Staphylococcus aureus FDA 209 P IFO
12732 (Staphyrococcus aureus FDA 209 P IFO 1273
2)

(Operation method) The same procedure as in Example 1 was carried out except that the Staphylococcus aureus was used instead of the Escherichia coli and the normal agar medium for detection was used instead of the desoxycholate agar medium for detection. The operation was performed to evaluate the bactericidal effect. The results are shown in Table 2 below.

Example 5 The bactericidal effect of the present bactericide against Bacillus cereus was examined. The same fungicide and medium as used in Example 3 were used.

(Bacterium used: Bacillus cereus IAM 12605)

(Operation method) The procedure was the same as in Example 1 except that the Bacillus cereus was used in place of the Escherichia coli and the normal agar medium for detection was used in place of the desoxycholate agar medium for detection. Then, the bactericidal effect was evaluated.
The results are shown in Table 2 below.

Example 6 The fungicidal effect of the fungicide of the present invention on Vibrio parahaemolyticus was examined. The composition of the medium used is shown below. The same germicide as in Example 1 was used.

(Strain used: Vibrio parahaemolyticus) Vibrio parahaemolyticus IFO 12711 (Vibrio parahaemolyticus FDA 209 P IFO 1271)
1)

(IFO designation No. 702 medium: medium for Vibrio parahaemolyticus) Polypeptone (manufactured by Wako Pure Chemical Industries) 0.5% by weight Yeast extract (manufactured by Wako Pure Chemical Industries) 0.2% by weight Magnesium sulfate (Katayama) 0.1% by weight Artificial seawater 75.0% by weight (manufactured by Jamarin Laboratory) Water Residue Adjusted to pH 7.0

(TCBS agar medium for detection: Eiken Chemical Co., Ltd.) Yeast extract 0.5% by weight Peptone 1.0% by weight White sugar 2.0% by weight Bovine bile powder 0.5% by weight Sodium cholate 0.3% by weight 1.0% by weight of sodium chloride 1.0% by weight of sodium citrate 0.7% by weight of sodium thiosulfate 0.1% by weight of iron citrate 0.004% by weight of bromothymol blue 0.004% by weight of thymol blue Agar 1. 5% by weight ion-exchanged water Residual pH adjusted to 8.8

(Operating method) Except for using the above Vibrio parahaemolyticus in place of the above-mentioned Escherichia coli, using the above-mentioned liquid medium for Vibrio parahaemolyticus as a liquid medium for preparing a specimen and the above-mentioned TCBS agar medium as an agar medium for detection, respectively. Was operated in the same manner as in Example 1 to evaluate the bactericidal effect.
The results are shown in Table 2 below.

[0063]

Table 1 Death rate (%) Name of bacterial cell General pathogenicity Salmonella E. coli E. coli O157 S. thyphimurium S. enteritidis treatment time 199.70 100 99.98 100 (min) 2 99.98 100 100 -3 99.98 100 100 100 5 100 100 100 100 100 10---100

[0064]

Table 2 Death rate (%) Cell name Yellow Bacillus cereus Enterococcus staphylococcus Vibrio Treatment time 199.0 100 100 (min) 2---399.6 100 100 5 99.7 100 100 10-100 100

As shown in Tables 1 and 2, the bactericide of the present invention was able to sufficiently sterilize various bacteria in a short time, and exhibited an excellent bactericidal effect.

Example 7 A mixture was prepared using various surfactants so as to have a component composition of a concentrated bactericide, and the degree of dissolution of the surfactant was examined. The component composition and the surfactant used are shown below.

(Concentrated type fungicide: pH 1.1) D, L-lactic acid 35% by weight Phosphoric acid 12% by weight Surfactant 0.25% by weight Sodium hydroxide 1.2% by weight Water

(Surfactant) (1) Kiraya extract: saponin (2) Glycerin fatty acid ester (manufactured by Sakamoto Yakuhin Kogyo) (3) Sucrose fatty acid ester (manufactured by Mitsubishi Chemical Foods Corporation)

(Method) Each component was added to 45 ml of water so as to have the above-mentioned concentrations, and the pH was adjusted while mixing. The degree of dissolution of the surfactant was visually determined, and the case where the mixture was transparent was evaluated as ○, and the case where the components were separated was evaluated as ×. The results are shown in Table 3 below.

[0070]

[Table 3] Surfactant Solubility degree Kiraya extract (saponin) ○ Glycerin fatty acid ester × Sucrose fatty acid ester ×

As shown in Table 3, when glycerin fatty acid ester and sucrose fatty acid ester were used, the two surfactants were separated and suspended on the surface of the solution. For this reason, when this solution was allowed to stand, the components did not become a uniform germicide. On the other hand, when the Quillaya extract (saponin) was used, the mixed solution was brown and transparent, and the respective components were uniformly dissolved, and a mixed concentrated fungicide could be obtained.

The bactericide using saponin obtained in Example 7 was diluted 100-fold with water, and the bactericidal effect against pathogenic Escherichia coli was examined in the same manner as in Example 2 above. Even when the treatment time by sterilization was one minute, the mortality was 100%, indicating a sufficient sterilizing effect.

Example 8 and Comparative Example Bacteria adhered to brussels sprouts were sterilized with a germicide. The bactericide and the composition of the medium used are shown below.

(Bactericide: pH 2.25) Saponin added Saponin-free D, L-lactic acid 0.35% by weight 0.35% by weight phosphoric acid 0.12% by weight 0.12% by weight sodium hydroxide 0.012% by weight % 0.012% by weight Kiraya extract: Saponin 0.025% by weight-water residue residue

(Normal agar medium for detection: Eiken Chemical Co., Ltd.) Meat extract 0.5% by weight Peptone 1.0% by weight Sodium chloride 0.5% by weight Agar 1.5% by weight Purified water residue pH 7.0 Adjustment

(Phosphate buffer solution) A solution of 0.20 mol / l disodium hydrogen phosphate · 12H ₂ O and a solution of 0.20 mol / l potassium dihydrogen phosphate were mixed and adjusted to pH 6.8.

(Method) A commercially available brussels sprouts was cut into approximately three equal parts with its root at the center, and each section was used as a sample for Examples, Comparative Examples and Controls.

The examples were carried out as follows. First, all the pieces were placed in a γ-ray sterilizing stoma filter for homogenizer MIX-1 (manufactured by Toppan Printing Co., Ltd.), and the weight of the sections was measured in advance. Then, 150 ml of the saponin-added germicide was added into the filter,
A sterilization treatment was performed by allowing the mixture to stand for a minute.

After the sterilization treatment, the bactericide was removed from the filter, and 100 ml of the sterilized phosphate buffer was added to bring the sprouts into sufficient contact. Then, the filter was set in a homogenizer MIX-1 (Blender Mix 1 type: manufactured by AES Laboratory), and the sections were homogenized for 120 seconds.

This homogenized solution was filtered, and the obtained filtrate sample was collected in a test tube and immediately cooled in ice water.
After cooling, 0.5 ml of the filtrate specimen was diluted with sterilized saline, and 0.5 ml of the specimen dilution was added to a sterilized petri dish. And in the petri dish,
8 m of the sterilized ordinary agar medium for detection, kept at 50 ° C.
of the sample and thoroughly mixed with the sample diluent. After the agar medium was solidified, the dish was covered and cultured at 30 ° C. for about 42 hours, and the number of colonies grown on the agar medium was counted. The control was treated in the same manner as described above except that the sterilization treatment with a germicide was not performed, and the number of colonies was counted.

The comparative example was treated in the same manner as in the above example except that the saponin-free fungicide was used instead of the saponin-added fungicide, and the number of colonies was counted. Similarly, treatment was performed on a total of 15 brussels sprouts, and the number of colonies was counted. In addition, the control and the comparative example with respect to each Example used the sample cut out from the same brussels sprouts, respectively.

By substituting the number of colonies and the weight of the section measured as described above into the following equation (Equation 2), the number of viable bacteria per gram of the section sample was determined. In addition, the bactericidal rate was determined by substituting the viable cell count per 1 g of the section sample and the viable cell count per 1 g of the control section into the following equation (Equation 3). The results are shown in Table 4 below.

[0083]

## EQU2 ## The number of viable bacteria per gram of section sample a = (y × z) ×
(1 / 0.5) × [(100 + x) / x] x: weight of section sample (g) y: number of colonies (pieces) z: dilution ratio (times)

[0084]

[Equation 3] Bactericidal rate b (%) = [(BA) / B] × 100 A: Number of viable bacteria per gram of section sample B: Number of viable bacteria per gram of control section sample

[0085]

[Table 4] Control Example Comparative Example aab ab (× 10 ⁶ ) (× 10 ⁶ ) (%) (× 10 ⁶ ) (%) 1 12.0 4.08 66 4.25 65 2 21.8 8.27 62 10.2 53 3 2.63 0.25 90 1.88 28 4 10.3 0.21 98 7.25 30 5 1.86 0.87 54 1.06 44 6 11.1 3.07 72 3.82 66 7 15.3 6.45 58 8.91 42 8 5.14 2.12 59 3.62 30 9 17.9 3.74 79 10.2 43 10 8.72 1.97 77 2.48 71 11 8.17 1.73 79 3.06 63 12 10.8 2.85 74 3.13 71 13 9.42 1.75 81 5.99 36 14 4.06 2.59 36 3.18 22 15 8.10 2.82 65 3.90 52

As shown in Table 4 above, in the examples and comparative examples, the viable cell count was reduced as compared with the control viable cell count using a section sample prepared from the same brussels sprouts.
Although the bactericidal effect was confirmed, the examples showed a better bactericidal rate than the comparative example. From this, it can be said that the bactericide containing an organic acid and an inorganic acid further contains a surfactant, saponin, so that the bactericidal effect is improved. The growth number a of the control was different for each brussels sprouts.
It is presumed that the number of adhering bacteria is different.

[0087]

As described above, the disinfectant of the present invention has good contact with the object to be disinfected, can be disinfected in a short time, and does not affect the quality of foods and the like. Sterilization can be performed sufficiently at low cost. Therefore,
For example, even in the case of sterilizing food or the like having a water-repellent surface, sufficient sterilization can be performed in a short time and with a small amount.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification symbol FI theme coat ゛ (Reference) A23L 3/3508 A23L 3/3508 3/358 3/358 (72) Inventor Akio Tanaka Kotobukicho, Tondabayashi-shi, Osaka 3-713-1 (72) Inventor Masanori Miyamoto 2-1.8-8 Koda, Tondabayashi-shi, Osaka

Claims (8)

[Claims] A fungicide comprising at least one substance selected from organic acids and salts thereof, at least one substance selected from inorganic acids and salts thereof, and saponin. 2. The content of at least one substance selected from organic acids and salts thereof is in the range of 0.05 to 91% by weight, and the content of at least one substance selected from inorganic acids and salts thereof is The fungicide according to claim 1, wherein the saponin content is in the range of 0.0005 to 6.0% by weight. 3. The content of at least one substance selected from organic acids and salts thereof is in the range of 5 to 91% by weight, and the content of at least one substance selected from inorganic acids and salts thereof is 1% by weight. The fungicide according to claim 2, wherein the content is in the range of 0.5 to 86% by weight and the saponin content is in the range of 0.05 to 6.0% by weight. 4. The fungicide according to claim 1, wherein the saponin is a saponin derived from Quillaja. 5. The organic acid is at least one organic acid selected from the group consisting of lactic acid, acetic acid, malic acid, citric acid, tartaric acid, ascorbic acid, gluconic acid, fumaric acid and adipic acid. , Lactic acid, acetic acid, malic acid, citric acid, tartaric acid, ascorbic acid, gluconic acid,
The fungicide according to any one of claims 1 to 4, which is a salt of at least one organic acid selected from the group consisting of fumaric acid and adipic acid. 6. The inorganic acid is at least one inorganic acid selected from the group consisting of phosphoric acid, pyrophosphoric acid, polyphosphoric acid, sulfuric acid and hydrochloric acid, and the salt thereof is phosphoric acid, pyrophosphoric acid, polyphosphoric acid, sulfuric acid A fungicide according to any one of claims 1 to 5, which is a salt of at least one inorganic acid selected from the group consisting of hydrochloric acid. 7. The disinfectant according to claim 1, wherein the organic acid is lactic acid and the inorganic acid is phosphoric acid. 8. The method according to claim 1, wherein the pH is not more than 3.0.
The germicide according to any one of the above.