JP2000128796A - Hemostatic - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hemostatic derived from a natural plant, having excellent biological safety, economic efficiency and hemostatic ability, useful for simple frogeye, senile frogeye, rheumatic frogeye, etc., by making the hemostatic include an extract of a plant of the genus Rhodiola as an active ingredient. SOLUTION: This hemostatic contains powder and/or an extract preferably using the whole plant of a plant of the genus Rhodiola being preferably Rhodiola sacra as an active ingredient. The effective amount of the hemostatic per time is preferably 450-600 mg as a standard and the hemostatic is administered three times daily. The extract is prepared, for example, in the case of the extract of Rhodiola sacra with an alcohol, by extracting 1 kg powder of Rhodiola sacra with preferably 3-5 L of an alcoholic solvent such as ethyl alcohol, under heating or cooling, filtering and concentrating the filtrate at <=45 deg.C under reduced pressure and completely distilling away the alcoholic solvent to give a dried essence.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to a natural plant which is known to have a strong platelet aggregation effect specific to an alcoholic extract in a plant belonging to the genus Chongjing genus, which has been known as a crude drug having a hemostatic effect since ancient times. The present invention relates to a high-performance hemostatic agent derived from a product.

[0002]

2. Description of the Related Art The fact that Hongjingtian has a hemostatic effect is also described in "China Pharmaceutical Dictionary" (Shanghai Chemical Technology Publishing), a typical Chinese medicinal plant dictionary. However, as a conventional hemostatic agent using Hongjing Heaven, a method of using the powder of Heijing Heaven or ingesting Heijing Heen with water has been used.

[0003] As the hemostatic agent, many other synthetic and other natural products are known in addition to the aforementioned Kokukeiten. Bleeding that is not caused by platelet abnormalities is one of intractable purpura, and there is a strong demand for natural, safe and effective crude drugs.

Needless to say, if blood leaks, physical strength is consumed. Therefore, in order to recover physical strength and maintain health, a material that can be easily taken daily, not as a medicine, has been widely demanded. In this regard, traditional folk medicines of each ethnicity have a long history of use and are promising sources. This is because, despite the difference in efficacy and effect, the safety of being used for many years can be said to be higher than that of synthetic new drugs.

[0005]

SUMMARY OF THE INVENTION The present invention relates to a simple purpura, senile purpura, rheumatic purpura, and diabetic purpura, which are considered to have enhanced vascular permeability in bleeding resulting from damage to the blood vessel wall. The point is to find a natural material having biosafety and strong hemostatic ability that can respond to diseases and the like.

In order to develop a hemostatic agent having biosafety and strong hemostatic ability, the present inventors conducted research on a large number of folklore herbs using washed platelets of rabbits, which are usually used for examining hemostatic agents. Stacked. The present inventor has conducted research on red-kage genus plants, which are said to have a haemostatic effect, especially as a result of tradition. It has been surprisingly found that when an alcohol extract is used instead of a liquid-based system, the effect is several times longer than in the past.

The present invention has been completed based on the above findings. INDUSTRIAL APPLICABILITY According to the present invention, Rhodio genus (Rhodio) derived from natural plants and having excellent biosafety, economic efficiency, and hemostatic ability
la, Crassulaceae) provided is a hemostatic agent comprising an alcohol extract of a plant as an active ingredient.

[0008]

SUMMARY OF THE INVENTION In summary, the present invention provides a hemostatic agent comprising an alcoholic extract of a plant of the genus Rhodoyla, such as Rhodiola sacra, as an active ingredient. It relates to the agent.

Hereinafter, the technical structure and embodiments of the present invention will be described in detail.

[0010] The alcohol extract of the red vine spp., Which is the active ingredient of the hemostatic agent of the present invention, is exemplified as follows. In the following, examples are given in the order of plant names (place names, production places). R. algi
da / Aomi, Kaihoku, Kaisai), Karako-Kingkeiten (R. algi)
da var. Tangutica / Aomi, Sichuan), Nishikawa Benkei (R. alsia / Sichuan), Koza Benkei (R.
dumulosa / Sichuan, Gansu), Ohana Kokeiten (R.e.)
urphylla / North and Southwestern Yunnan, Tibet, Long Whip Red Scenic Spot (R. fastigata / North and Southwestern Tibet), Longscale Red Scenic Spot (R. gelida / Utenyama), Seven Bean Red Scenic Spot (R. henryi / Gansu, Henan, Hubei, Sichuan, Guizhou), R.heterodonta
/ Xinjiang, Tibet), R. kirilow
ii / Hebei, Shanxi, Yunnan, Sichuan, Tibet), Ritsu Quadrifida (R. quadrificida / Gansu, Qinghai, Xinjiang, Sichuan, Tibet), R. sachaline
nsis / Heilongjiang, Jilin), Kaji Kokeiten (all dialects) (R.
sacra / northwestern Yunnan, southeastern Tibet), ▲ Zu ▼ Red Jingtian (R. scabrida / western Sichuan, northwestern Yunnan),
Coarse stalk red scape (R. wallichiana / northwest of Yunnan, southeast of Tibet)
chiana var. cholaensis / Aomi,
Northwest of Yunnan), R. yunnanensi
s / northwestern part of the lake). The localities of the Red Jing genus plants are famous in Tibet, Sichuan, Yunnan, etc. of the People's Republic of China.

In the hemostatic agent of the present invention, all the parts of the genus plants of the genus Red Jingen, such as the Red Jing heaven, can be used, but it is particularly economical to use whole plants. It is efficient.

In order to prepare an alcohol extract (extract) from the above-mentioned raw material (Red Species Plant), it may be extracted in a desired manner. For example, as the alcohol extraction of Hongjingtian,
3 to 5 liters of an alcohol solvent is added to 1 kg of Kungjing heaven powder, and the mixture is extracted under warm or cold temperature. 8 for heating
After leaving at 0 to 90 ° C. for 3 hours and in the case of cold temperature at room temperature for 3 days, each was filtered, and the filtrate was concentrated under reduced pressure at 45 ° C. or less.
The alcohol solvent is completely distilled off to obtain a dry extract (alcohol extract powder). In the present invention, desired excipients, lactose, starch and the like may be added to the extract powder to form granules or tablets.

In the present invention, the solvent used for the alcohol extraction is aqueous alcohol or alcohol. As the alcohol, ethyl alcohol exhibits specificity in the hemostatic effect. In the present invention, as demonstrated in the examples described below, the ethyl alcohol extract of the plant belonging to the genus Chongjing, exhibits an extremely strong (selective) hemostatic ability as compared to other alcohol extracts, water extracts, and the like. This is quite surprising.

In the present invention, it is inappropriate to use methyl alcohol (methanol) as the alcohol extraction medium. Although methyl alcohol is inexpensive, even if methanol is distilled out and concentrated by vacuum treatment, HPLC
It is detected by an advanced analytical instrument such as, for example, and cannot be regarded as a preferable extraction medium in view of the situation of daily drinking. Further, other alcohols such as propyl alcohol and butyl alcohol have a property of extracting a component having a smaller polarity. On the other hand, ethanol
It has the property of extracting not only components extracted with propyl and butyl alcohol but also components soluble in water.
Therefore, ethanol has a high extraction power and a high extraction yield.

[0015] In the hemostatic agent of the present invention, it is preferable to take 450 to 600 mg three times a day as a guide, in view of the experimental results, as an effective amount for one time.

[0016]

The present invention will be described in more detail with reference to the following examples. It goes without saying that the present invention is not limited to the embodiment.

(1) Preparation of Hongjingtian Alcohol Extract The method of extracting Hongjingtian extract is as follows. After rough cutting, 5 l of a solvent is added to 1 kg of Hongjingtian and heated at 80 to 90 ° C. for 3 hours. .
Thereafter, the mixture was filtered, and the solvent was completely distilled off from the filtrate under reduced pressure at 45 ° C. to obtain a dry extract. The yield of ethyl alcohol extract was extremely high at 40 to 45%. On the other hand, the yield of n-propyl alcohol or n-butanol extract is 6 to 8%, and the present invention is also unique in terms of the difference in yield depending on the extraction medium.

(2) Test of Hemostasis by Rabbit Platelets The experiment of haemostasis was carried out by using the usual test method, aggregation by rabbit platelets as an index. That is, rabbit washed platelets were prepared in the presence of 1 mM calcium chloride by using 25 to 100 μg of a sample such as the red-kageten alcohol extract shown in Table 1 below.
/ Ml, and the change in turbidity due to platelet condensation was measured and evaluated. As a control, 10 μM of platelet aggregation factor (PA
The intensity of the turbidity change in F) was set to 100%.

The results are shown in Table 1 below. As is clear from Table 1, the hemostatic ability of the Hongjingtian alcohol extract of the present invention is specific compared to other alcohol extracts and water extracts.

[0020]

[Table 1]

(3). Effect test of Kungjing Heaven on CV6209 which is a specific antagonist of PAF (platelet coagulation factor) The purpose of this study is to examine the effect of the extract on platelet aggregation. That is, this test is for confirming whether the platelet coagulation factor component is contained in the red blood and checking whether the hemostatic factor of the red blood is acting like PAF.

This test method was carried out by pretreatment of washed platelets with 30 μM of CV6209 in the presence of 1 mM calcium chloride for 2 minutes, and then observing the agglutination rate in the case of adding the Kokukeiten extract. In addition, 100% aggregation is 1
This was the case with the addition of 0 μM PAF. The platelet aggregometer used was from Nikko Bioscience. The results are shown in Table 2 below.

[0023]

[Table 2]

FIGS. 1 and 2 show typical aggregation patterns. 1 and 2 show (i). Washed platelets were treated with 1 mM Ca
After pretreatment with 30 μM and 10 μM CV6209 for 2 minutes in the presence of Cl ₂ , (ii) 10 μM PAF (FIG. 1)
(Case) or 50 μg / ml of all lipstick keiten ethyl alcohol extract (Case in FIG. 2), and (iii). The agglomeration rate at that time is shown in a chart.

FIG. 1 and FIG. 2 show that the light transmittance before aggregation is reduced with the agar-like reaction liquid due to the aggregation of platelets, and the light transmittance is deteriorated. 1 and 2, the higher the concentration, the more agglutinated, in other words, the more effective the hemostasis.

[0026]

According to the present invention, there is provided a hemostatic agent which specifically enhances the hemostatic ability of a plant belonging to the genus Benkei, such as Benkei, which is known to have a hemostatic action. The hemostatic agent of the present invention is composed of an ethyl alcohol extract of a plant belonging to the genus Red Jingen, and has a hemostatic ability and an extraction yield (productivity) in comparison with other alcohol extracts or water extracts. And exhibit significant specificity.

[Brief description of the drawings]

FIG. 1 is a graph showing the effects of PAF (platelet coagulation factor) and its specific antagonist CV6209 on platelet aggregation.

FIG. 2. PAF on platelet aggregating effect of Zenkakei Keiten
It is a figure which shows the influence of specific antagonist CV6209.

Claims (2)

[Claims] 1. A hemostatic agent comprising, as an active ingredient, an alcohol extract of a plant belonging to the genus Rhodiola (Rhodiola). 2. The plant of the genus Red Jingen, which is Rhodobio
2. The hemostatic agent according to claim 1, which is a lacara).