JP2000095628A - Hydrogen sulfide production inhibitor from sulfate reducing bacterium and hydrogen sulfide production inhibiting composition for food and drink or the like containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a hydrogen sulfide production inhibitor safe for humans and livestock and usable for suppressing the generation of hydrogen sulfide in alimentary canals and feces and suppressing the generation of the hydrogen sulfide in corrosion of metallic equipment such as a pipeline or a tank in industrial facilities such as oil wells or various kinds of refining factories or wastewater treatment in various kinds of factories or public sewage treatment. SOLUTION: This inhibitor contains one or more kinds selected from the groups of vegetable extracts known as a herb such as Curcumae Rhizoma, a crude drug or a food material and the group of food additives such as acetyl eugenol as an active ingredient.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria and a composition containing the same, which inhibits the production of hydrogen sulfide such as food and drink.

[0002]

2. Description of the Related Art Since sulfate-reducing bacteria produce hydrogen sulfide from sulfate, an inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria in an environment or facility where the generation of hydrogen sulfide is a problem is useful. Conventionally, the so-called hydrogen sulfide production inhibition, which suppresses the generation of hydrogen sulfide in the corrosion of metal equipment such as pipelines and tanks in industrial facilities such as oil wells and various refinery factories, and also in the treatment of various factory wastewaters or public sewage. Various bactericides and antibiotics are used as agents.

[0003] Disinfectants and antibiotics include chlorine, hypochlorous acid, chlorine dioxide, ozone, formaldehyde, glutaraldehyde, acrolein, cetylpyridinium chloride, cetyltrimethylammonium chloride,
Cocodiamine, triazine, methylenebiscyanic acid, isothiazolone and the like are used (“Biotechnology Han”).
dbooks ・ 8 Sulfate-Reducing Bacteria ”, Chapter 10“ Control in Industrial Settings ”, Plenum Publish
ing co., New York, pp. 265-292, 1995).

[0004] Also, anthraquinones (JP-A-6-27)
9207), beet red, cochineal pigment, monasque pigment, crystal violet, fuchsin, gentian violet B, etc.
4) is known to be effective in suppressing the production of hydrogen sulfide by sulfate-reducing bacteria.

[0005] By the way, the presence of sulfate-reducing bacteria has been confirmed not only in industrial facilities but also in the intestines of humans and livestock. Particularly in humans, a remarkable number of sulfate-reducing bacteria are found in their feces. Many of them are Desulfovibrio
ovibrio) (Gibson et al., FEMS Microbiology Ecol).
ogy, 86, pp. 103-112 [1991]). 9-338 from 1 g (dry weight) of stool per day due to the action of sulfate-reducing bacteria
4 nmol of hydrogen sulfide is generated (Nollet and Verstraete, En
vironmental Monitoring and Assessment, 42, 113
-131 [1996]).

[0006] Hydrogen sulfide not only corrodes metals, but also is a substance that is perceived as an unpleasant putrid odor to humans, and is a main cause of fecal and fart odor (Moore
Et al., Gastroenterology, 93, pp. 1321-1329 [19
87]) In addition, it is highly toxic to humans and livestock, and it has been suggested that hydrogen sulfide generated in the digestive tract may cause injury by inhibiting metabolism of gastrointestinal mucosal cells.

[0007] In particular, hydrogen sulfide produced by sulfate-reducing bacteria is
It has been suggested to contribute to ulcerative colitis (Will
iam et al., Gastroenterology, 104, pp. 802-809 [1993]), and there is a demand for a method for suppressing the production of hydrogen sulfide by sulfate-reducing bacteria in the digestive tract. . If an antibiotic is used, there are problems such as safety and emergence of resistant bacteria such as side effects during long-term administration.

[0008]

SUMMARY OF THE INVENTION It is an object of the present invention to provide a pharmaceutical composition which is safe even when administered to humans and livestock, has no risk of emergence of resistant bacteria, has no side effects, and has a high level of activity in the digestive tract and feces. A hydrogen sulfide production inhibitor that can effectively suppress the generation of hydrogen sulfide. In addition, pipelines and tanks in industrial facilities such as oil wells and various refinery factories, where the generation of hydrogen sulfide is a problem. It is an object of the present invention to provide a hydrogen sulfide production inhibitor that can be used to suppress the generation of hydrogen sulfide in the corrosion of metal equipment such as the above, and in the treatment of various factory wastewaters or public sewage.

[0009] The inventors of the present invention have studied various plant extracts and food additives which are known as herbs, herbal medicines or food materials and are considered safe for humans and livestock and have no side effects. The present inventors have conducted intensive studies on the inhibition of the production of hydrogen sulphide from methane and completed the present invention.

[0010]

Means for Solving the Problems The inhibitors of hydrogen sulfide production from sulfate-reducing bacteria according to the present invention include: Gansu (ginger), cat nip, snapdragon (muscle bone),
Guava leaves, coffee leaves, comfrey, sayou (chain sun),
Stevia, spearmint, sabori, soyou (soba),
Taklan (Sawaran), tarragon, dandelion, chives, dillweed, basil, parsley, patchouli, halucone, hyssop, peppermint, hops, white horehound, myrtle, motherwort, marjoram, yakmosou (yasu-mokuso), yaro, swordfish ),
The active ingredient is one or more selected from the group of plant extracts such as sagebrush (mosaic leaf), raspberry, ryokyo (ginger), lemon balm and rose.

The hydrogen sulfide production inhibitor from sulfate-reducing bacteria according to the present invention includes acetyleugenol, p-anisaldehyde, (+)-borneol, (-)-borneol, camphene, carvacrol, β-citronellol, p-
Cymene, 3-decanol, n-decanol, dihydrocarbon, dihydrocarbyl acetate, 3,4-dimethoxy-1-allylbenzene, 3,7-dimethyl-1-octanol, 3-ethoxy-4-hydroxybenzaldehyde, furfural, geraniol , Isoeugenol, isomentone, lavandulol, L-limonene, L
-Menthol, d-neomenthol, mentfuran, methyl benzoate, 2-methylbutylisovalerate, methyl salicylate, nerol, 2-nonanone, nopol, 3
-Octanol, perillyl alcohol, 3-phenyl-
1-Prapanol, β-pinene, trans-sabinene hydrate, α-terpinene, γ-terpinene, terpinolene, thymol, ethyl gallate, 3-tert-butyl-4
-Hydroxyanisole, erythrosine, phloxine,
One or more selected from the group of food additives such as rose bengal is the active ingredient.

Further, the inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria according to the present invention contains, as an active ingredient, one or more selected from the group of the above-mentioned plant extracts and food additives.

The composition for inhibiting the production of hydrogen sulfide such as food and drink according to the present invention contains one or more of the above-mentioned inhibitors for the production of hydrogen sulfide from sulfate-reducing bacteria. Foods and drinks include soft drinks, chewing gum, candy, chocolate and other confections, frozen desserts, dairy products, alcoholic beverages, meats, deodorant foods and other foods. In addition to food and drink, pet food, livestock feed, and the like can be mentioned.

It is not necessary to particularly limit the place of origin and form of the plant from which the various extracts are obtained, or the method of extracting the extract, and the plant may be used as a raw material as it is. Alternatively, the raw material may be subjected to necessary treatment such as drying, shredding, and pulverization.

For example, in the extraction, 1 to 10 times the amount of extraction solvent is added to the raw material, and the mixture is heated at a temperature of 25 to 100 ° C. for 30 minutes to 5 minutes.
The extraction is carried out by heating for a time. As the extraction solvent,
Water, methanol, ethanol, ethyl acetate, acetone,
A single solvent may be selected from ether, n-hexane and the like, and a single solvent may be used, or a mixed solvent obtained by mixing two or more optionally selected ones in an arbitrary ratio may be used. In addition, the extract thus obtained may be used as it is,
Heat concentration and vacuum concentration may be used. Further, if necessary, the powder may be used after being dried and powdered by a spray drying method, a freeze drying method or the like.

As the above-mentioned various food additives, those obtained from extracts of natural origin or synthetic products can be used as appropriate.

Further, the inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria according to the present invention may be used alone, or may be used as a mixture of two or more of them. Can be used in admixture with a pharmaceutical carrier or diluent, and can be used as a medicament in any dosage form. Examples of the dosage form include tablets, granules, fine granules, hard capsules, soft capsules and oral liquid preparations.

The dosage of the inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria according to the present invention cannot be unequivocally defined because it varies depending on the number of sulfate-reducing bacteria, sulfate-reducing activity, etc., but the total dose per day is , 0.2 mg to 10 for humans and livestock
0 mg / kg of body weight, preferably 1 mg to 60 mg / kg of body weight.

As described above, the inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria according to the present invention is already known as an herb, crude drug or food material, and is considered to be safe for humans and livestock and has no side effects. Since extracts and food additives of various plants are used as active ingredients, they can be administered to humans and livestock with confidence. In addition, in the corrosion of metal equipment such as pipelines and tanks in industrial facilities such as oil wells and various refinery factories, and in the treatment of various factory wastewater or public sewage, that is, in environments and facilities where generation of hydrogen sulfide is a problem. It can also be used to suppress the generation of hydrogen sulfide.

[0020]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below with reference to a plurality of examples and test examples, but the scope of the present invention is not limited thereby.

[Example 1-Echinacea purpurea ethanol extract] The whole plant of Japanese persimmon was dried in the sun, 100 g of the obtained dry raw material was pulverized, and 1 liter of ethanol was added to the obtained pulverized material, and the mixture was placed in a flask. And extracted at room temperature for 24 hours. Furthermore, the ethanol extract obtained by filtration was evaporated to dryness under reduced pressure using an evaporator, to obtain 14.4 g of a persimmon ethanol extract.

Example 2 Comfrey 50% Ethanol Extract 200 g of dried comfrey leaves were pulverized, and 1 liter of 50% ethanol was added to the obtained pulverized product, which was then placed in a flask equipped with a reflux condenser. At 85 ° C. for 3 hours under reflux. The filtrate obtained by filtration was concentrated under reduced pressure by an evaporator, and further freeze-dried to obtain Comfrey 50.
27.1 g of a 1% ethanol extract were obtained.

Example 3-Peppermint water extract Peppermint leaves were subjected to steam distillation to remove the essential oil portion, and then dried and pulverized. And extracted with stirring at 60 ° C. for 2 hours. The extract obtained by filtration was concentrated to dryness by an evaporator, and 8.9 g of a peppermint water extract was obtained.
I got

Example 4 Basil Ethanol Extract Basil leaves were vacuum-dried at 50 ° C., and then the dried leaves were pulverized. To 500 g of the obtained pulverized product, 2 liters of ethanol was added, followed by reflux condenser. At 70 ° C.
The mixture was stirred and extracted for hours. The extract obtained by filtration was concentrated with an evaporator, and further dried at 60 ° C. with a vacuum dryer to obtain 5.0 g of a basil ethanol extract.

Example 5 Parsley 50% Ethanol Extract 300 ml of 50% ethanol was added to 30 g of dried leaves of parsley, and the mixture was placed in a flask equipped with a reflux condenser and subjected to reflux extraction at 85 ° C. for 3 hours. The extract obtained by filtration was concentrated by an evaporator, and further freeze-dried under vacuum to obtain 5.7 g of a parsley 50% ethanol extract.

Example 6 Hyssop Water Extract 100 g of dried leaves of hyssop was crushed, 1 liter of water was added to the obtained crushed product, and the mixture was placed in a flask equipped with a reflux condenser.
Extraction was performed for 3 hours. The extract obtained by the filtration was freeze-vacuum-dried to obtain 20.6 g of Hyssop water extract.

Example 7-Hop water extract 20.1 g of dried hop cones were crushed.
0 ml of water was added, and the mixture was put into a beaker and extracted with stirring at 80 ° C. for 3 hours. The extract obtained by the filtration was freeze-vacuum dried to obtain 5.2 g of a hop water extract.

Example 8-White horehound water extract 100 g of the dried ground part of white horehound was ground, 1 liter of water was added to the obtained ground material, and the mixture was placed in a flask equipped with a reflux condenser. Extracted at 80 ° C. for 3 hours.
24. The extract obtained by filtration is freeze-dried under vacuum,
3 g of white horehound water extract was obtained.

Example 9-Water extract of mother wort 100 g of dried mother wort was ground, 1 liter of water was added to the obtained ground product, and the mixture was placed in a flask equipped with a reflux condenser and extracted at 80 ° C. for 3 hours. did. The extract obtained by the filtration was freeze-vacuum dried to obtain 28.4 g of a mother wort water extract.

Example 10-Soybean 50% ethanol extract 100 g of Soybean (Soyo leaf) was pulverized, and 500 ml of 50% ethanol was added to the obtained pulverized product. Time extracted. The extract obtained by filtration was freeze-dried under vacuum to obtain 28.4 g of a 50% ethanol extract of Soybean.

[Hydrogen Sulfide Production Inhibition Test of Sulfate-Reducing Bacteria] Desulfovibrio desulfuricans (DSM642) was used as the sulfate-reducing bacterium.
The medium was prepared by dissolving the reagents shown in Table 1 in 1 liter of water,
After adjusting to 6.8, 100% carbon dioxide gas was passed through until the red color of the medium disappeared, and 10 ml was dispensed into a hunt gate tube and sealed. This medium was sterilized by an autoclave at 121 ° C. for 15 minutes. The sterilized medium was inoculated with 0.2 ml of a culture of Desulfovibrio desulfuricans. Further, the sample was added as a water or ethanol solution (the control was not added with the sample) and cultured at 37 ° C. for 43 hours. This test was always performed in an atmosphere of 100% carbon dioxide. In such a test, when the sulfate-reducing bacteria survive and grow in the medium, the sulfate is reduced and hydrogen sulfide is released into the headspace gas. In this test, the sulfuric acid reduction reaction was confirmed by analyzing hydrogen sulfide in the headspace gas by gas chromatography. The inhibition rate of hydrogen sulfide production was determined by the following formula. Inhibition rate (%) = (CS) / C) × 100 (C: amount of hydrogen sulfide in control headspace gas, S:
Hydrogen sulfide in headspace gas with sample added)

[0032]

[Table 1]

As described above, a screening test was performed on the plant extract according to the present invention including the extracts of Examples 1 to 10 and the food additive, and the results are shown in Tables 2 to 6.

[0034]

[Table 2]

[0035]

[Table 3]

[0036]

[Table 4]

[0037]

[Table 5]

[0038]

[Table 6]

[0039] These results indicate that the hydrogen sulfide production inhibitor of the present invention can be used in the gastrointestinal tract and feces of humans and livestock, in the corrosion of metal equipment in industrial facilities such as oil wells and various refineries, and in the treatment of various industrial wastewaters. It shows that it has an inhibitory effect on the sulfate-reducing bacterium that is the causative bacterium of hydrogen production.Furthermore, the hydrogen sulfide production inhibitor of the present invention expresses the effect of inhibiting hydrogen sulfide production from the sulfate-reducing bacterium by its active ingredient. By inhibiting the production of hydrogen sulfide, it prevents corrosion of metals,
It suggests reducing toxicity to humans and livestock, preventing and treating inflammation such as ulcerative colitis, and suppressing the generation of gastrointestinal tract contents, feces and fart smell.

Example 11-Candy A candy was prepared according to the following formulation. Granulated sugar 45 parts Water syrup (DE.42) 50 parts Water 20 parts Oyster extract EtOH extract (Example 1) 3 parts

Example 12-Chewing gum Chewing gum was prepared according to the following formulation and according to a commonly used conventional method. Gum base 20 parts Sugar 56 parts Syringe 13 parts Glucose 10 parts White horehound water extract 5 parts Comfrey 50% EtOH extract (Example 2) 3 parts Softener 1 part Fragrance 0.5 part

[Example 13-Chewing gum] A chewing gum was prepared in the same manner as described above under the following formulation. Gum base 20 parts sugar 56 parts starch syrup 13 parts glucose 10 parts parsley 50% EtOH extract (Example 5) 5 parts softener 1 part flavor 0.5 part

Example 14-Chocolate Chocolate was prepared according to the following recipe and according to a commonly used conventional method. Cocoa bitter 20 parts Cocoa butter 17 parts Sugar 43 parts Full fat milk powder 20 parts Hyssop water extract (Example 6) 1 part Fragrance 0.2 part

Example 15-Biscuit A biscuit was prepared according to the following formulation and according to a commonly used conventional method. Soft flour 100 parts Shortening 30 parts Powdered sugar 32 parts Ginger syrup (DE.42) 5 parts Skim milk powder 1.8 parts Salt 1.1 parts Peppermint water extract (Example 3) 1 part Swelling agent 0.45 parts

Example 16-Tablets Tablets were prepared according to the following formulation according to a commonly used conventional method. Lactose 100 parts Potato starch 100 parts Soybean 50% EtOH extract (Example 10) 60 parts

Example 17-Beverage A beverage was prepared according to the following formulation and according to a commonly used conventional method. Orange juice 30 parts Isomerized sugar 15.24 parts Citric acid 0.1 part Vitamin C 0.04 parts Fragrance 0.31 part Hop water extract (Example 7) 0.1 part Water 55 parts

Example 18-Toothpaste A toothpaste was prepared according to the following formulation and according to a commonly used conventional method. Calcium carbonate 50 parts Glycerin 20 parts Carboxymethyl cellulose 2 parts Sodium lauryl sulfate 2 parts n-decanol 1 part Saccharin 0.1 parts Basil EtOH extract (Example 4) 8 parts Chlorhexidine 0.01 parts Water 94 parts

Example 19 Powders Powders were prepared according to the following formulation and according to a commonly used conventional method. Lactose 62 parts Potato starch 25 parts 2-nonanone 3 parts Campfen 1 part

[0049]

As is apparent from the above description,
The hydrogen sulfide production inhibitor according to the present invention is one kind selected from a group of herbs such as turmeric (Utsukin), a group of plant extracts known as crude drugs or food materials, and a group of food additives such as acetyleugenol. Or, because it contains two or more kinds of active ingredients, it is safe for humans and livestock, not only suppresses hydrogen sulfide production in the digestive tract and feces, but also pipes in industrial facilities such as oil wells and various refinery factories. It can also be used to suppress the corrosion of metal equipment such as lines and tanks, the production of hydrogen sulfide in the treatment of various industrial wastewaters or the treatment of public sewage, and has a remarkable suppression or inhibition effect.

The composition for inhibiting hydrogen sulfide production according to the present invention comprises:
Since it contains the hydrogen sulfide production inhibitor, food and drink,
Useful as pet food or livestock feed.

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Claims (4)

[Claims] 1. Turmeric (Utsukin), Orgon, Oregano, Rensenso (Rensenso), Japanese persimmon, Karako-boku (Karakoboku), Cardamom, Kankyo (Ginger), Catnip, Kingfisher (muscle bone), Guava leaf, Coffee leaf, Comfrey, Sayou (Shoyo), Stevia, Spearmint, Sabori, Soyou (Soyo), Takran (Sawaran), Tarragon, Dandelion, Chives, Dilweed, Basil, Parsley, Patchouli, Halukong, Hyssop, Peppermint, Hop, Select from a group of plant extracts such as white horehound, myrtle, mother wort, marjoram, yam moth (mother grass), yaro, sycamore (yangmei), raspberry, ryokyo (ryang), lemon balm and rose Done 1
An inhibitor for the production of hydrogen sulfide from sulfate-reducing bacteria, comprising one or more species as active ingredients. 2. Acetyl eugenol, p-anisaldehyde, (+)-borneol, (-)-borneol, camphene, carvacrol, β-citronellol, p-cymene, 3-decanol, n-decanol, dihydrocarbon, Hydrocarbyl acetate, 3,4-dimethoxy-1-allylbenzene, 3,7-dimethyl-1-octanol, 3-ethoxy-4-hydroxybenzaldehyde, furfural, geraniol, isoeugenol,
Isomentone, lavandulol, L-limonene, L-menthol, d-neomenthol, mentfuran, methyl benzoate, 2-methylbutylisovalerate, methyl salicylate, nerol, 2-nonanone, nopol, 3-octanol, perillyl alcohol , 3-phenyl-1-
Prapanol, β-pinene, trans-sabinene hydrate, α-terpinene, γ-terpinene, terpinolene,
The active ingredient is one or more selected from the group of food additives such as thymol, ethyl gallate, 3-tert-butyl-4-hydroxyanisole, erythrosine, phloxine, rose bengal and the like. An inhibitor of hydrogen sulfide production from sulfate-reducing bacteria. 3. Hydrogen sulfide from a sulfate-reducing bacterium, characterized in that one or more selected from the group of the plant extract of claim 1 and the food additive of claim 2 is an active ingredient. Production inhibitors. 4. A composition for inhibiting hydrogen sulfide production, such as food and drink, comprising the inhibitor for producing hydrogen sulfide from sulfate-reducing bacteria according to any one of claims 1 to 3.