JP2000083471A - Medium composition for cultivating paecilomyces japonica plant worm and cultivation of paecilomyces japonica by using the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a medium composition for cultivating Paecilomyces Japonica, having excellent anticancer, immuno-potentiation, and nourishing and strengthening effects, and to provide a method for cultivating the Paecilomyces Japonica capable of cultivating more simply, inexpensively and rapidly than the conventional artificial cultivating methods. SOLUTION: This medium composition for cultivating Paecilomyces Japonica consists of 25-35 wt.% saw dust, 25-35 wt.% rice bran, 5-15 wt.% insect chrysalis and 25 wt.% water. Also, the method of cultivation is provided by inoculating a fungal seed of the Paecilomyces Japonica on the above medium composition, culturing at 20-30 deg.C for 5-7 days, then growing its fruit body at 18-22 deg.C under >=5,000 lux and >=95% humidity, and harvesting before the fruit body forms spores.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a kind of medicinal mushroom,
A medium composition for cultivating a snow caterpillar, which has excellent anticancer effect, immunopotentiating effect and nutrient tonic effect, and cultivating a snow caterpillar cultivating medium, and using the same, can be more easily, inexpensively, and rapidly cultivated than existing artificial cultivation methods. The present invention relates to a method for cultivating a snow caterpillar.

[0002]

2. Description of the Related Art Snow caterpillars are insects and other hosts.
It is a kind of mushroom of the genus Cordyceps that forms fruiting bodies when the mycelium is filled in the host body after the hypha grows and causes the host to die, and it is in the host body in winter and in the summer in the host. A mushroom that pierces the insect body and belongs to the ascomycetes. The fruit body of this mushroom has various shapes such as a matchstick shape, the asci are conical, and most ascospores are thread-like.

[0003] In nature, fungi exist as hyphae or spores in the ground or on the ground.
doptera), beetles (Coleoptera),
In the order Hemiptera, Odonata, etc., it invades the body throughout the larval, pupal and adult stages, and infests it.

[0004] In Chinese medicine, the fruiting body of Cordyceps sinensis is used as a remedy for tuberculosis or jaundice, an antidote for opiate poisoning and a tonic, and recently clinical studies have demonstrated that it has high anticancer properties. ing.

[0005] Cordycepin, which is extracted from the fruiting body of Cordyceps sinensis, is known to inhibit the growth of streptococci, staphylococci, dermatophytes and the like. For these reasons, snow caterpillars are widely known as medicinal mushrooms.

Studies on artificially forming fruit bodies of this mushroom have been reported in various countries such as the United States. Particularly in China, studies on the use of artificially formed fruit bodies as Kampo medicines have been actively conducted. Made and in practical use. Research into the formation of population fruiting bodies has been actively conducted in Korea in recent years, and various researches for commercial use are already in progress.

Among the techniques relating to the production of a mushroom culture medium related to the present invention, there is Korean Patent Publication No. 96-036902 relating to the cultivation of general mushrooms, which includes sawdust, compost, wheat, barley, rice bran and the like. The present invention relates to a method for producing a series of seed fungi for cultivating mushrooms used as a medium. Other techniques for mushroom cultures include Korean Patent Publication No. 94-008557.
There is also a medium for mushroom cultivation and a method for producing the same, which also uses sawdust as a main component up to 85%, uses a small amount of rice bran and okara as sub-components, and uses it as a medium for general mushrooms. It is. However, there is no known example of using a general medium for mushrooms as a medium for cultivating snowy cordyceps. Further, the medium composition of the present invention is different from the medium composition of the present invention in that the composition ratio and the water content are different, and the culture properties are different depending on the mixing time of the components and the presence or absence of void formation between the media components by the method. I do.

Techniques related to the cultivation of Cordyceps sinensis include, for example, Korean Patent Publication No. 98-224, an insect seed culture medium for Cordyceps sinensis and a method for cultivating a box thereof, and Korean Patent Publication No. 98-2241, a medium composition for Cordyceps sinensis cultivation. There are things and cultivation methods. However, there is a difference between these and the present invention in the components of the medium. Korean Patent Publication No. 98-13 discloses a method for cultivating Cordyceps spp. Using insects, and Korean Patent Publication No. 97-68810 discloses a seed culturing method and cultivation of Cordyceps cordyceps using silkworm pupae, respectively. ing. However, the present invention is much different from the above in that the present invention allows far more convenient cultivation of Cordyceps sinensis by preparing the medium composition once. A new technique and a medium composition method for large-scale artificial culture of Cordyceps sinensis disclosed in Korean Patent Publication No. 97-68809 using Chinese herbal medicines and the present invention have in common that a liquid inoculum is used. Is completely different from the present invention using agricultural by-products.

[0009] As a conventional technique for artificial cultivation of Cordyceps sinensis, there is a method using silkworms. That is, the surrounding environment of the live silkworm larva is changed to induce the silkworm's epidermis to become weaker, and then the spores of Cordyceps sinensis are directly inoculated by an aseptic method. Then, the larvae are grown to pupae while culturing so that the spores can germinate and easily penetrate into the silkworm body. The silkworm forms a cocoon when it becomes a pupa, but the cocoon is cut and opened one by one, and after the pupa in the cocoon is taken out, the mushroom is grown while maintaining the temperature and humidity suitable for the fruiting body to grow. It is a method of harvesting. However, this method requires a preliminary operation before cultivation of mushrooms to grow and maintain healthy silkworms not infected by the disease. In addition, a complicated operation of incising the cocoons one by one by hand and taking out the pupae in the cocoons is required. In addition, as a result, production costs increase due to an increase in labor costs due to manual labor, and the silkworms themselves are expensive, resulting in high costs. Therefore, if snow caterpillars are cultivated by many farmers in the future, prices are expected to decrease due to the large supply of snow caterpillars. Can not be.

[0010] In other methods of artificially cultivating snow caterpillars, such as a cultivation method using various cereal flours and silkworm flour, it is necessary to purchase cereal flour, resulting in high production costs, and as a result, the price is reduced. Competitiveness decreases. In order to increase the yield rate of fruiting bodies, multi-step culture methods are not only complicated, but also non-specialized farmers who do not have microbiological Therefore, it is difficult to spread the multistage culture method of mushrooms to general farmers.

[0011]

SUMMARY OF THE INVENTION According to the present invention, rice bran, sawdust and pupae generated as by-products in a farmer are used to increase the rate of return on production cost of snowy Cordyceps, which is sold at a higher price than ordinary mushrooms. , Maintain its profitability even during mass production, and enable mass production by simplifying the culture method,
Facilitate the spread of the method of cultivating snow caterpillars to general farmers.

That is, an object of the present invention is to provide a medium composition for cultivating a snowy cordyceps, which is a kind of medicinal mushroom and has an excellent anticancer effect, an immunopotentiating effect and a nourishing tonic effect. Another object of the present invention is to provide a method for cultivating a snow caterpillar, which can be cultivated more easily, cheaply and quickly than an existing artificial cultivation method.

[0013]

In order to achieve the above object, according to the present invention, 25 to 35% by weight of sawdust is used.
A medium composition for cultivating Paecilomyces japonica, comprising 5 to 35% by weight of rice bran, 5 to 15% by weight of insect pupae, and 25 to 35% by weight of water. Provided.

[0014] The medium composition comprises sawdust,
It is composed of rice bran, pupa, and water, all of which are inexpensive, so that the cordyceps canis can be cultured at low cost.

In the present invention, the composition is composed of 25 to 35% by weight of sawdust, 25 to 35% by weight of rice bran, 5 to 15% by weight of insect pupae, and 25 to 35% by weight of water. After inoculating a seed culture of a cordyceps sinensis into a medium for cultivating a cordyceps sinensis, and culturing it at 20-30 ° C. for 5-7 days,
A method for cultivating a cordyceps sinensis, comprising growing fruit bodies at a temperature of 22 ° C., an illuminance of 500 lux or more, and a humidity of 95% or more, and harvesting the fruit bodies before the fruit bodies produce spores.

This culturing method is very simple, and can be carried out at low cost because an inexpensive medium composition is used. Furthermore, since the period from the start of the culture to the harvest is shorter than that of the conventional culture method, mass production is possible during the same period as compared with the conventional culture method.

[0017]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, preferred embodiments of a medium composition for cultivating snow and cordyceps of the present invention will be described in detail.

The medium composition for cultivating snowy cordyceps of the present embodiment comprises sawdust, rice bran, insect pupae, and water.

Sawdust is a component that plays a role in coagulating rice bran described later when given an appropriate amount of water, and its ratio is set to 25 to 35% by weight of the total weight of the medium composition. . The reason will be described later. As the saw dust, wood scrap or corroded wood generated when cutting wood with a saw or the like is used.

Rice bran is a component that acts as a carbon source. The presence of the carbon source promotes the growth of Cordyceps sinensis as compared to the absence of the carbon source. The ratio of rice bran is set at 25-35% by weight.

The pupa of the insect is the host of the snow caterpillar.
In other words, it is a host until the fungus of the snow caterpillar grows as a fruit body. The percentage of insect pupae is 5-1
Set to 5% by weight.

Insects are not particularly limited as long as they are capable of parasitizing snow caterpillars, but silkworms are preferred because they are inexpensive and readily available.

Water is a component that provides the medium composition with the moisture necessary for the growth of the cordyceps sinensis (fungi) and also provides the above-mentioned sawdust with appropriate moisture, and is set at 25 to 35% by weight.

The medium composition composed of these components can be obtained by first mixing and stirring sawdust, rice bran and water, and then dispersing the pupae on the ground surface. Voids are formed in the ground of the medium composition. These voids play a role in facilitating the growth and proliferation of mycelium and preventing the contamination of other bacteria such as diseased bacteria. The formation of this void is greatly affected by the composition ratio of sawdust, rice bran and water.

Further, the composition ratio of sawdust, rice bran and water is as follows:
Affects fruiting body growth. That is, by selecting the composition ratio of sawdust, rice bran and water, the fruit body can be significantly grown before spores are formed.

The ratio of each component is set in the above-mentioned range for the reason described above. That is, if the proportion of each component is out of the above range, voids may not be formed. Also, before the fruiting body grows significantly,
This is because spores may be formed. In addition,
The weight ratio of sawdust, rice bran and water is preferably the same.

Next, a preferred embodiment of the method for cultivating a snow caterpillar according to the present invention will be described in detail.

In the culture method of the present embodiment, the culture is carried out using the above-described medium composition for cultivating snowy cordyceps.

First, the above medium composition is sterilized. Examples of the sterilization treatment include a treatment by heating and pressurization in a sterilizer.

Next, a seed fungus of Cordyceps sinensis is inoculated aseptically into the medium composition and cultured at 20 to 30 ° C. Under these conditions, the mycelium of Cordyceps sinensis spreads throughout the medium composition in about 5 to 7 days.

Next, the fruiting body is grown while suppressing the growth of mycelium. That is, the temperature is lowered to 18-22 ° C., and an illuminance of 500 lux or more and a humidity of 95% or more are supplied. Under these conditions, the fruiting bodies are 2
It grows to 6-8 cm in about 2 days. No spores have yet been formed in this fruiting body, and at this stage, snow caterpillars are harvested.

When the fruiting body is used for medicinal purposes, the fruiting body may be dried in a cool and dark place.

The inoculum contains 1-5% by weight of yellow-white bran and 0.1-0.5% by weight of soybean flour before inoculation into the medium, and is heated and pressurized. It is preferable to use those cultured in a sterilized liquid medium. This is because the inoculum cultured in such a liquid medium produces more fruiting bodies than the inoculum not cultured in the liquid medium.

As described above, the culture method of the present embodiment is very simple. Further, in the conventional culturing method, it takes about 40 days or more from inoculation of the inoculum to the medium composition to harvest of fruiting bodies, whereas in the culturing method of the present embodiment, it is about 30 days. That is, it is possible to circulate the repetitive cycle of cultivation and harvesting of the snow caterpillar in a short period of time. Therefore, during the same period, a larger amount of fruiting bodies can be produced as compared with the conventional culture method.

[0035]

EXAMPLE In this example, two types of snow caterpillars (Paecilomyces japonica KFFC-C660 and Paecilomy) were purchased from the Korean Cordyceps sinensis bank at Gangwon National University in Korea.
cesjaponica KFFC-C240) was used.

1. Inoculum culture After receiving a strain of Cordyceps sinensis (Paecilomyces japonica), the strain was inoculated into a PDA (Potato Dextrose Agar, Difco) medium, subcultured, and used as an inoculation source to inoculate a liquid medium.

This inoculation source was prepared using a PD with a cork borer.
Remove from medium A and contain 3.0% (w / v) of yellow-white sugar and 0.3% (w / v) of soybean flour, and
The mycelium was inoculated into a liquid medium sterilized at 15 ° C. and 15 atm for 15 minutes, and cultured at 25 ° C. for 7 days while rotating at 150 rpm. This was used as a liquid inoculum.

2. Manufacture of a medium composition for cultivating a snow caterpillar, a summer grass, 40 g of rice bran, which is a by-product of a farmer, as a carbon source, and 40 g of sawdust, which is industrial waste, are placed in a mushroom culture bottle, and water 40 g is added.
After adding ml, the mixture was stirred and mixed evenly. Finally,
10 g of silkworm pupae were dispersed on the entire surface of the medium, the lid of the bottle was closed, and the mixture was sterilized with a sterilizer at 121 ° C. and 15 atm for 60 minutes to obtain a grounding composition for snow and cordyceps.

3. Cultivation of Cordyceps sinensis The liquid inoculum was inoculated in a sterile environment into the above ground composition for cordyceps sinensis using a sterilized pipette. Thereafter, the cells were grown at 25 ° C. until the mycelium spread throughout the medium composition. This period was about 5-7 days.

After the mycelium had spread throughout the medium composition, the temperature was lowered to 20 ° C. in order to suppress the growth of the mycelium and grow only the fruiting bodies. Further, light of 500 lux or more was irradiated, and a humidifier supplied a sufficient humidity of 95% or more into the incubator.

On the 22nd day after inoculation of the liquid inoculum into the medium composition, the Cordyceps sinensis grew to about 6 to 8 cm, and on the 25th day, fruiting bodies grew to near the mouth of the cultivation bottle. Further, on the 30th day, spore formation was observed in the fruit body.
Therefore, the harvesting of the fruiting bodies takes place before the fruiting bodies produce spores, i.e. on days 22 to 29 after inoculation of the liquid inoculum,
Preferably, it is performed on the 25th to 29th days.

For comparison, 80 g of brown rice was used as a carbon source, 120 ml of water was mixed and stirred, and 10 g of silkworm pupae were dispersed on the surface. Cordyceps was cultured. Table 1 summarizes the growth of mycelium with time and temperature in the medium composition and brown rice medium of the present embodiment.

[0043]

[Table 1]

From Table 1, it can be seen that the cultivation method of the present embodiment allows the fruiting body to grow larger in a short period of time as compared with the conventional culturing method. It is clear that the period is short.

[0045]

As described above, the medium composition for cultivating snow and cordyceps of the present invention is inexpensive and easily available because all of its components are agricultural by-products. In particular, pupae are easy to store and manage, and have many advantages in producing a medium composition. In addition, the medium composition for cultivating a snow caterpillar of the present invention is inexpensive to produce and has a high yield of mushrooms. In addition, the method for cultivating the cordyceps sinensis of the present invention using the medium composition is very simple, so that cultivation is easy even for ordinary farmers who do not have microbiological knowledge. Therefore, this culture method is expected to be easily spread widely to general farmers and to greatly contribute to revitalization of the rural economy.

 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Monki Hyuk Korea, Gyeonggi-do, Sowon, Lee Yang-guk, Song Jog-dong, 386-9 (72) Inventor Kim Hee-Jung South Korea, Seoul, Songpa , Iyami-Sildon, 86 Asia Sensochon Apartment 7-305

Claims (6)

[Claims] 1. 25 to 35% by weight of sawdust, 25 to 35% by weight of rice bran, 5 to 15% by weight of insect pupae, 25 to 3% by weight
A medium composition for cultivating snow caterpillars (Paecilomyces japonica), comprising 5% by weight of water. 2. 25 to 35% by weight of sawdust, 25 to 35% by weight of rice bran, 5 to 15% by weight of insect pupae, 25 to 3% by weight.
A medium for cultivating a snow caterpillar, which is composed of 5% by weight of water, is inoculated with a seed fungus of a snow caterpillar,
After culturing for 雪 7 days, growing a fruiting body at 18-22 ° C., an illuminance of 500 lux or more, and a humidity of 95% or more, harvesting before the fruiting body produces spores. . 3. The culture method according to claim 2, wherein as the medium composition, sawdust, rice bran and water are mixed and agitated, then the pupae of the insects are dispersed on the surface, and then sterilized by heating and pressurizing. A method for cultivating a snow caterpillar, which is characterized by using the following. 4. The culture method according to claim 3, wherein the weight ratio of the sawdust, rice bran and water is the same, and the sterilization treatment is performed at 121 ° C. and 15 atm for 60 minutes. Cultivation method of snow caterpillar. 5. The culture method according to claim 2, wherein the inoculum is 1 to 5% by weight of yellow-white sugar and 0.1 to 5% by weight.
A method for cultivating a cordyceps sinensis, which comprises using a liquid medium containing 0.5% by weight of soy flour and sterilized by heating and pressurizing in advance. 6. The method according to claim 5, wherein the sterilization is performed at 121 ° C. and 15 atm for 10 to 20 minutes.