JP2000051351A - Protein dissolving agent for artificial dialyser - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a protein dissolving agent which is excellent in protein dissolving performance, facilitates washing, and insignificantly exerts a bad influence on the environment by containing at least one kind selected from inorganic acid ammonium salt, ethanolamine salt and metallic salt, and setting the liquid property to alkalinity. SOLUTION: A protein dissolving agent is made by containing at least one kind selected from inorganic acid ammonium salt, ethanolamine salt and a metallic salt, and setting the liquid property to alkalinity. At this time, preferably, to stabilize the pH value, a pH moving inhibitor is contained and the pH value is regulated to 7 to 9. As an inorganic acid, for example, carbonic acid, bicarbonic acid, and phosphoric acid are used. As a pH regulator, alkali materials such as phosphoric acid, citric acid, acetic acid and so on are singly used or in a combination of two or more kinds. Further, in addition to the pH moving inhibitor, various additives such as a germicide, a surfectant and bleaching agent may be contained at need.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a protein dissolving agent for an artificial dialysis device, and more particularly, to an artificial dialysis agent having excellent protein solubility, excellent detergency, and less adversely affecting the environment due to drainage of a washing solution. The present invention relates to a protein solubilizer for a device.

[0002]

2. Description of the Related Art Various types of dialysis machines used for artificial dialysis use proteins or the like in pipes for a long period of time.
Pumps, silicon tubes, etc. become contaminated. These stains impede the flow of blood and dialysate, causing malfunctions of the apparatus and serving as a hotbed for the generation of bacteria. Therefore, it is necessary to wash the dialyzer prior to use.

Conventionally, as a cleaning agent for an artificial dialysis device,
Sodium hypochlorite, a formulation in which a surfactant is mixed with sodium hypochlorite, acetic acid, and the like are used.

[0004] However, these detergents corrode the materials of artificial dialysis equipment, especially metallic materials, and in formulations containing a surfactant, they have poor detergency and require a long time for cleaning, which leads to an increase in working efficiency. Not only does it lower the water, but it also has problems such as the discharged cleaning water having an adverse effect on the environment.

In recent years, as a means for solving these problems, use of a cleaning agent containing peracetic acid as a main agent has been studied. However, in the case of using peracetic acid, compared with the conventional washing with sodium hypochlorite, the protein dissolving ability is slightly inferior, and recently, the pore size of the hollow fiber membrane of the artificial kidney tends to increase, A large amount of protein in the blood is excreted into the dialysate, and depending on the method of artificial dialysis and the number of times of artificial dialysis performed per day, the protein adheres to the artificial dialysis machine, which causes malfunction of the dialysis machine Problems such as are being brought up.

On the other hand, JP-A-9-75688 discloses that
A detergent for an artificial dialysis device containing a surfactant, a bactericide or a bactericide and a hydrophilic organic solvent, and containing an organic acid and a salt thereof such that the pH is 2 to 5 is described. .
However, since such a detergent contains a surfactant as an essential component, the detergency is poor as described above, and thus the cleaning efficiency is greatly reduced due to the long time required for the cleaning, and the discharged cleaning water is reduced. There is a risk of deteriorating the environment. Furthermore, there is a possibility that the environment of the organic solvent may be deteriorated. Furthermore, since organic acids are causative substances of BOD, they also have an adverse effect on the environment. Further, pH 2 to 5 is out of the range of pH 5 to 9, which is a general drainage standard, and it is hard to say that it is preferable.

[0007]

SUMMARY OF THE INVENTION The present invention solves the above-mentioned problems of the prior art and provides a protein dissolving agent for an artificial dialysis device which has excellent protein solubility, is easy to wash, and has little adverse effect on the environment. Is what you do.

[0008]

Means for Solving the Problems The inventors of the present invention have conducted intensive studies in view of the above-mentioned circumstances, and as a result, the problems of the prior art described above have been solved by containing a salt of an inorganic acid and making the liquid alkaline. The present inventors have found that it is possible to provide a protein dissolving agent for an artificial dialysis device which has excellent protein solubility, is easy to wash, and has a small adverse effect on the environment, and has completed the present invention.

That is, the present invention provides an ammonium salt of an inorganic acid,
At least one selected from ethanolamine salts and metal salts
A protein dissolving agent for an artificial dialysis device, which contains a seed and has a liquid alkaline property. In a preferred embodiment, the pH is further adjusted to stabilize the pH value.
It contains a migration inhibitor. In a more preferred embodiment, the pH value is adjusted to 7 to 9.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The inorganic acid used in the present invention is not particularly limited, and examples thereof include carbonic acid, bicarbonate, phosphoric acid, silicic acid and boric acid. Examples of salts of these inorganic acids include ammonium salts; ethanolamine salts such as monoethanolamine, monoisopropanolamine, diethanolamine, diisopropanolamine, triethanolamine and triisopropanolamine; sodium, magnesium, aluminum, potassium, calcium, etc. And these are used alone or in combination of two or more. The content of the inorganic acid salt is usually 0.1 to 30.0% by weight, preferably 1.0 to 20.0%.
% By weight. If it is less than 0.1% by weight, protein solubility is not sufficient, and if it exceeds 30.0% by weight, salts of inorganic acids may precipitate at low temperatures, which is not preferable.

[0011] The protein lysing agent of the present invention is adjusted so that the liquidity is alkaline, and is preferably adjusted to pH 7-9. When the liquidity becomes acidic, the protein solubility decreases. PH 7 to 9 is a general drainage standard, p
It is within the range of H5 to H9, and is preferable in that it is environmentally friendly.

The pH adjuster used to adjust the liquid property to alkalinity is not particularly limited. For example, acidic substances such as phosphoric acid, citric acid, acetic acid, hydrochloric acid, glycolic acid, lactic acid, tartaric acid, ammonia water, water Sodium oxide,
Examples thereof include alkaline substances such as potassium hydroxide and monoethanolamine, which are used alone or in combination of two or more.

The protein lysing agent of the present invention preferably contains a pH shift inhibitor to suppress the shift of pH. Examples of such a pH shift inhibitor include ED
Examples include sequestering agents (chelating agents) such as TA-2Na, EDTA-4Na, hydroxyethanediphosphonic acid, and hydroxyethanediphosphonic acid-4Na, and these may be used alone or in combination of two or more.

The content of the pH shift inhibitor is usually 0.01
To 2.0% by weight, preferably 0.1 to 1.0% by weight. If the amount is less than 0.01% by weight, the effect of inhibiting the pH shift is not sufficient, and the effect such as the solubility of the protein may change over time. Is not desirable because it cannot be expected and increases the cost.

The protein solubilizing agent of the present invention may further comprise, if necessary, one or more of various additives such as a bactericide, a surfactant, an oxidizing or reducing bleaching agent, a wetting agent, a thickener, and a dye. Two or more can be contained.

Examples of fungicides include chlorhexidine gluconate, isopropylmethylphenol, diphenhydramine hydrochloride, trichlorohydroxydiphenyl ether, trichlorocarbanilide, hexachlorophen, parachloromethaxylenol, benzothiazole,
α-bromocyanamide, halogenated phenol derivatives, benzalkonium chloride, didecyldimethylammonium chloride, dioctyldimethylammonium chloride, tetradecyldimethylbenzylammonium chloride, didecyldimethylpyridinium chloride, aminoethylglycine, cetyltrimethylammonium bromide and the like Cationic or zwitterionic compounds are mentioned, and these are used alone or in combination of two or more.

As the surfactant, an anionic surfactant, a nonionic surfactant and an amphoteric surfactant are used.

Examples of the anionic surfactant include linear alkylbenzene sulfonates, alkyl sulfates, alkyl ether sulfates, alkyl sulfonates, α-olefin sulfonates, alkyl phosphates and alkyl diphenyl ether disulfonates. , Alkyl ether fatty acid salts, acyl glutamates and the like.

Examples of the nonionic surfactant include polyoxyethylene alkyl ether, polyoxyethylene alkylphenol ether, fatty acid alkanolamide, polyoxyethylene hydrogenated castor oil, polyoxyethylene sorbitan fatty acid ester, and polyoxyethylene glyceryl monoalkylate. , Alkylamine oxide, polyoxyethylene polyoxypropylene block ether, ethylene glycol alkylate and the like.

Examples of the amphoteric surfactant include imidazolinium betaine, betaine alkylaminoacetate, betaine amidopropyl, alkylbetaine, and polyalkylaminoethylglycine.

The above surfactants may be used alone or in combination of two or more. It is desirable that surfactants are not used from the viewpoint of ease of cleaning and adverse effects on the environment,
Even if used, it is preferable to use as little as possible,
For example, the content is preferably 0.2% by weight or less.

Examples of the oxidized or reduced bleaching agents include potassium persulfate, sodium perborate, hydrogen peroxide, sodium percarbonate, sodium sulfite, sodium thiosulfate and the like. Used in combination of more than one species.

Examples of the humectant include sorbitol, glycerin, ethylene glycol, propylene glycol, hexylene glycol, 1,3-butylene glycol, dipropylene glycol, urea, pyrrolidone carboxylate, sodium lactate and the like. Are used alone or in combination of two or more.

As the above thickener, carboxymethyl cellulose (CMC), carboxy polymer, ethyl cellulose, methyl cellulose, xanthan gum, guar gum,
Gum arabic, dextrin, dialdehyde starch,
Ramzan gum and the like, and the pigments include flavono, carotene, purple root, chlorophyll and the like. These may be used alone or in combination of two or more.

The protein solubilizing agent for an artificial dialysis device obtained as described above is usually diluted about 35 to 50 times with water and used for washing the artificial dialysis device.

[0026]

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples, but these do not limit the present invention at all.

Examples 1 to 9 and Comparative Examples 1 to 5 The compounds shown in Table 1 were uniformly mixed at the compounding ratios shown in the table to produce protein solubilizers for artificial dialysis devices. Using the obtained protein solubilizer for artificial dialysis devices, the following methods were used to evaluate the protein solubility and the presence or absence of a shift in the pH value. Table 1 shows the results.

Protein solubility: Albumin and globulin, which are a kind of protein contained in blood, are dissolved in purified water at a ratio of 3: 2, and this is applied to a slide glass and naturally dried to obtain a test piece. 100 ml of the solution obtained by diluting the protein solubilizer 35 times was put into a beaker,
The test piece is immersed in this, the time required for the applied protein to dissolve is measured, and the effect is determined (unit: minute).

Shift of pH value: The pH value is measured 30 days after the production of the protein solubilizer.

[0030]

[Table 1]

From the results shown in Table 1, the protein lysing agents of the present invention represented by Examples 1 to 9 all dissolved the protein in less than 20 minutes, while those of Comparative Examples 1 to 3 in which the pH was in the acidic region. None of the protein lysing agents dissolved after more than 60 minutes. Further, the protein solubilizers of Comparative Examples 4 and 5 using an organic acid salt required 20 minutes or more to dissolve the protein, and were inferior in protein solubility as compared with the protein solubilizer of the present invention using an inorganic acid salt. You can see that it is.

The protein dissolving agent containing the pH shift inhibitor has no difference in the protein solubility, but the pH shift with the lapse of time is suppressed, and the effect such as the protein solubility is stable without change. I understand.

Examples 10 to 13 The compounds shown in Table 2 were uniformly mixed at the compounding ratios shown in the same table to produce a protein dissolving agent for an artificial dialysis device. Using the obtained protein solubilizer for artificial dialysis equipment, the detergency during water washing was evaluated by the following method. The protein solubilizer for artificial dialysis devices obtained in Examples 6 and 7 was evaluated in the same manner. Table 2 shows the results.

Detergency during washing with water: 35% protein solubilizer
Diluted 1-fold, filled in a silicon tube with an inner diameter of 8 mm and a length of 5 m, left for 1 hour, expelled the diluent with purified water, and foamed the cleaning solution when washing the inside of the silicon tube at a flow rate of 500 ml for 1 minute. Measure the time (minutes) required to cease.

[0035]

[Table 2]

From the results in Table 2, it can be seen that Examples 6 and 7 containing no surfactant did not foam at all (0 minutes) and had very good detergency, whereas the surfactant contained 0.2% by weight. In Example 10, foaming is stopped by washing for 2 minutes, and in Examples 11 to 13 containing 10% by weight of a surfactant, it takes 30 minutes, 45 minutes, and 40 minutes, respectively, which is a long time until the cleaning liquid stops foaming. Was required, and the cleaning property was poor. From the above results, it is desirable to use no surfactant from the viewpoints of detergency, i.e., shortening the cleaning time to enhance work efficiency and saving water resources, and even when used, it is preferably 2% by weight or less. It is desirable to keep the amount small. Further, since the surfactant has a bad biodegradability and adversely affects the environment when drained, it is preferable not to use the surfactant or to keep the surfactant in a small amount even when used. Note that the organic acids used in Comparative Examples 4 and 5 are also causative substances of BOD, and thus have an adverse effect on the environment.

[0037]

As described above, the protein solubilizer for an artificial dialysis device of the present invention exhibits excellent protein solubility and has good detergency. It has many features that it can save water resources at the same time as improving it, and that it does not adversely affect the environment when draining the wash water.

 ──────────────────────────────────────────────────続 き Continued on the front page F term (reference) 4C077 AA05 BB01 BB10 GG02 GG13 KK30 PP28 4D006 GA13 KC16 KD04 KD11 KD17 KD25 KD26 KD30 PC47

Claims (3)

[Claims] 1. An inorganic acid comprising at least one selected from ammonium salts, ethanolamine salts and metal salts,
A protein dissolving agent for an artificial dialysis device, wherein the solution has an alkaline property. 2. The protein solubilizing agent for an artificial dialysis device according to claim 1, further comprising a pH shift inhibitor for stabilizing the pH value. 3. The method according to claim 1, wherein the pH value is adjusted to 7 to 9.
Or the protein solubilizing agent for an artificial dialysis device according to 2.