ITTO20121155A1 - Microparticella multistrato comprendente fluorofori - Google Patents
Microparticella multistrato comprendente fluorofori Download PDFInfo
- Publication number
- ITTO20121155A1 ITTO20121155A1 IT001155A ITTO20121155A ITTO20121155A1 IT TO20121155 A1 ITTO20121155 A1 IT TO20121155A1 IT 001155 A IT001155 A IT 001155A IT TO20121155 A ITTO20121155 A IT TO20121155A IT TO20121155 A1 ITTO20121155 A1 IT TO20121155A1
- Authority
- IT
- Italy
- Prior art keywords
- layer
- fluorophore
- microparticle
- contact
- multilayer
- Prior art date
Links
- 239000011859 microparticle Substances 0.000 title claims 22
- 239000002773 nucleotide Substances 0.000 claims 11
- 125000003729 nucleotide group Chemical group 0.000 claims 11
- 239000000523 sample Substances 0.000 claims 6
- 108020004711 Nucleic Acid Probes Proteins 0.000 claims 4
- 230000000295 complement effect Effects 0.000 claims 4
- 239000002853 nucleic acid probe Substances 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 3
- 150000007523 nucleic acids Chemical class 0.000 claims 3
- 102000039446 nucleic acids Human genes 0.000 claims 3
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims 2
- 125000003903 2-propenyl group Chemical group [H]C([*])([H])C([H])=C([H])[H] 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 claims 2
- 150000001408 amides Chemical class 0.000 claims 2
- 239000000427 antigen Substances 0.000 claims 2
- 102000036639 antigens Human genes 0.000 claims 2
- 108091007433 antigens Proteins 0.000 claims 2
- 150000002148 esters Chemical class 0.000 claims 2
- 230000005284 excitation Effects 0.000 claims 2
- 239000000816 peptidomimetic Substances 0.000 claims 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims 2
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 claims 2
- 229920002554 vinyl polymer Polymers 0.000 claims 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
- G01N33/525—Multi-layer analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Claims (10)
- RIVENDICAZIONI 1. Microparticella multistrato (4) comprendente: - almeno un primo strato (5) comprendente un primo fluoroforo (51), - almeno un secondo strato (6) a contatto con il primo strato (5), e - almeno un terzo strato (7) a contatto con il secondo strato (6) e comprendente un secondo fluoroforo (71), dove - il primo fluoroforo (51) e il secondo fluoroforo (71) sono diversi, e - il primo strato (5) e il terzo strato (7) non sono a contatto l’uno con l’altro. 2. Microparticella multistrato (4) secondo la rivendicazione 1 comprendente inoltre: - almeno un quarto strato (8) a contatto con il terzo strato (7), e - almeno un quinto strato (9) a contatto con il quarto strato (8) e comprendente un terzo fluoroforo (91), dove - il terzo fluoroforo (91) è diverso dal secondo fluoroforo (71) e dal primo fluoroforo (51), e - il terzo strato (7) e il quinto strato (9) non sono a contatto l’uno con l’altro. 3. Microparticella multistrato (4) secondo la rivendicazione 1 o 2, avente dimensioni da 0,5 µm a 2 µm. 4. Microparticella multistrato (4) secondo una qualsiasi delle rivendicazioni precedenti, in cui ciascuno strato comprende esteri e ammidi dell’acido acrilico oppure dell’acido metacrilico oppure vinilici o allilici opzionalmente sostituiti. 5. Microparticella multistrato (4) secondo una qualsiasi delle rivendicazioni precedenti, comprendente una molecola fissata alla superficie dello strato più esterno e selezionata dal gruppo costituito da un anticorpo, un antigene, un enzima, una sonda nucleotidica, un peptide, e un peptidomimetico. 6. Microparticella multistrato (4) secondo la rivendicazione 5, in cui la molecola è fissata mediante un legame ammidico tra la molecola e la superficie dello strato più esterno. 7. Microparticella multistrato (4) secondo la rivendicazione 5 o 6, in cui la molecola fissata è una prima sonda (1) di acido nucleico da 10 a 14 basi, all’estremità 5’ della quale è legato almeno un quarto fluoroforo (11) avente lunghezza d’onda di eccitazione diversa dalla lunghezza d’onda di emissione del primo, secondo e terzo fluoroforo (51, 71, 91). 8. Microparticella multistrato (4) secondo la rivendicazione 7, comprendente inoltre almeno una seconda sonda (2) di acido nucleico da 35 a 50 basi, la seconda sonda (2) comprendendo, dall’estremità 5’ all’estremità 3’: - un primo segmento (21) di sequenza nucleotidica complementare alla prima sonda di acido nucleico (1), - almeno un quencher (23) per il quarto fluoroforo (11), e - un secondo segmento (22) di sequenza nucleotidica complementare ad almeno parte di una sequenza nucleotidica bersaglio (3). 9. Microparticella multistrato (4) secondo una qualsiasi delle rivendicazioni da 6 a 8 per uso nel rivelare una sequenza nucleotidica bersaglio (3) a singolo filamento di lunghezza da 15 a 100 basi. 10. Microparticella multistrato (4) secondo la rivendicazione 9 in cui la sequenza nucleotidica bersaglio (3) è da 20 a 40 basi. TITLE: MULTILAYER MICROPARTICLE COMPRISING FLUOROPHORES 1. A multilayer microparticle (4) comprising: - at least one first layer (5) comprising a first fluorophore (51), - at least one second layer (6) in contact with the first layer (5), and - at least one third layer (7) in contact with the second layer (6) and comprising a second fluorophore (71), where: - the first fluorophore (51) and the second fluorophore (71) are different, and - the first layer (5) and the third layer (7) are not in contact one with the other.
- 2. The multilayer microparticle (4) according to claim 1, further comprising: - at least one fourth layer (8) in contact with the third layer (7), and - at least one fifth layer (9) in contact with the fourth layer (8) and comprising a third fluorophore (91), where: - the third fluorophore (91) is different from the second fluorophore (71) and from the first fluorophore (51), and - the third layer (7) and the fifth layer (9) are not in contact one with the other.
- 3. The multilayer microparticle (4) according to claim 1 or 2, having a size from 0.5 µm to 2 µm.
- 4. The multilayer microparticle (4) according to any of the preceding claims, wherein each layer comprises esters and amides of acrylic acid or of methacrylic acid or vinyls or allyls, which are optionally substituted.
- 5. The multilayer microparticle (4) according to any of the preceding claims, comprising a molecule fixed to the surface of the outermost layer and selected from the group consisting of an antibody, an antigen, an enzyme, a nucleotide probe, a peptide, and a peptidomimetic.
- 6. The multilayer microparticle (4) according to claim 5, wherein the molecule is fixed by means of an amide bond between the molecule and the surface of the outermost layer.
- 7. The multilayer microparticle (4) according to claim 5 or 6, wherein the fixed molecule is a first nucleic acid probe (1) from 10 to 14 bases, at the 5’ end of which at least one fourth fluorophore is bound (11) having an excitation wave length other than the emission wave length of the first, second and third fluorophore (51, 71, 91).
- 8. The multilayer microparticle (4) according to claim 7, also comprising at least one second nucleic acid probe (2) from 35 to 50 bases, the second probe (2) comprising, from the 5’ end to the 3’ end: - a first nucleic acid segment (21) complementary to the first nucleic acid probe (1), - at least one quencher (23) for the fourth fluorophore (11), and - a second nucleotide sequence segment (22) complementary to at least part of a target nucleotide sequence (3).
- 9. The multilayer microparticle (4) according to any of claims from 6 to 8 for use in detecting a single strand target nucleotide sequence (3) having a length from 15 to 100 bases.
- 10. The multilayer microparticle (4) according to claim 9, wherein the target nucleotide sequence (3) is from 20 to 40 bases.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT001155A ITTO20121155A1 (it) | 2012-12-27 | 2012-12-27 | Microparticella multistrato comprendente fluorofori |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT001155A ITTO20121155A1 (it) | 2012-12-27 | 2012-12-27 | Microparticella multistrato comprendente fluorofori |
Publications (1)
Publication Number | Publication Date |
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ITTO20121155A1 true ITTO20121155A1 (it) | 2014-06-28 |
Family
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Family Applications (1)
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IT001155A ITTO20121155A1 (it) | 2012-12-27 | 2012-12-27 | Microparticella multistrato comprendente fluorofori |
Country Status (1)
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Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1568699A (en) * | 1975-11-10 | 1980-06-04 | Minnesota Mining & Mfg | Colour-coded identifier microparticles |
US6455157B1 (en) * | 1998-11-21 | 2002-09-24 | Simons Druck & Vertrieb Gmbh | Method for protecting and marking products by using microparticles |
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US6620360B1 (en) * | 1996-04-10 | 2003-09-16 | Simons Druck & Vertrieb Gmbh | Process for producing multilayered microparticles |
US20050152967A1 (en) * | 2003-03-28 | 2005-07-14 | Pfab, Lp | Dynamic variable release |
US20050189255A1 (en) * | 2004-02-27 | 2005-09-01 | Safian John W. | Plastic packaging having embedded micro-particle taggants |
US20060193877A1 (en) * | 2005-02-28 | 2006-08-31 | Pfab, Lp | Compositions and methods of making sustained release liquid formulations |
US20090175949A1 (en) * | 2007-12-20 | 2009-07-09 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Microparticles for delivery to cells and/or tissues |
US20110262364A1 (en) * | 2007-09-22 | 2011-10-27 | Hua Wang | Fluorescent Particles Comprising Nanoscale ZnO Layer and Exhibiting Cell-Specific Toxicity |
-
2012
- 2012-12-27 IT IT001155A patent/ITTO20121155A1/it unknown
Patent Citations (9)
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GB1568699A (en) * | 1975-11-10 | 1980-06-04 | Minnesota Mining & Mfg | Colour-coded identifier microparticles |
US6620360B1 (en) * | 1996-04-10 | 2003-09-16 | Simons Druck & Vertrieb Gmbh | Process for producing multilayered microparticles |
US6455157B1 (en) * | 1998-11-21 | 2002-09-24 | Simons Druck & Vertrieb Gmbh | Method for protecting and marking products by using microparticles |
WO2003048726A2 (en) * | 2001-11-30 | 2003-06-12 | Tracking Technology Inc. | Taggants for products and method of taggant identification |
US20050152967A1 (en) * | 2003-03-28 | 2005-07-14 | Pfab, Lp | Dynamic variable release |
US20050189255A1 (en) * | 2004-02-27 | 2005-09-01 | Safian John W. | Plastic packaging having embedded micro-particle taggants |
US20060193877A1 (en) * | 2005-02-28 | 2006-08-31 | Pfab, Lp | Compositions and methods of making sustained release liquid formulations |
US20110262364A1 (en) * | 2007-09-22 | 2011-10-27 | Hua Wang | Fluorescent Particles Comprising Nanoscale ZnO Layer and Exhibiting Cell-Specific Toxicity |
US20090175949A1 (en) * | 2007-12-20 | 2009-07-09 | Arizona Board Of Regents Acting For And On Behalf Of Arizona State University | Microparticles for delivery to cells and/or tissues |
Non-Patent Citations (7)
Title |
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