ITMI20102081A1 - DERMATOLOGICAL AND / OR COSMETIC PREPARATIONS WITH ANTITHROSINASIC, ANTIOXIDANT, ANTIELASTASIC AND ANTICOLLAGENASIC ACTIVITIES - Google Patents

Description

DESCRIPTION

of the patent for industrial invention entitled:

â € œDERMATOLOGICAL AND / OR COSMETIC PREPARATIONS WITH ANTITHROSYNASIC, ANTIOXIDANT, ANTIELASTASIC AND ANTICOLLAGENASIC ACTIVITIESâ €

The present invention relates to dermatological and / or cosmetic preparations with anti-tyrosinase, antioxidant, anti-elastase and anti-collagenase activity.

More precisely, the invention relates to dermatological and / or cosmetic preparations based on silk proteins obtained from silkworms of particular breeds, relatively little widespread, which have been recognized as having antityrosinase, antioxidant and anti-elastase, and surprisingly anti-collagenic activity. effective and superior compared to other breeds of wider diffusion and common use.

Silk is a protein fiber of animal origin, in particular obtained from the silkworm (Bombyx mori), widely used not only for the packaging of textile products but also in biomedical, pharmaceutical and tissue engineering applications.

As known, the silk fiber is made up of two proteins: fibroin and sericin, which respectively represent the supporting structure and the protective coating of the fiber.

The properties of these proteins have been extensively investigated, resulting in numerous applications in various sectors, including the medical one.

For example, the use of fibroin as a suture thread is now consolidated, while its use in biomedical applications such as coating of materials, in tissue engineering for the repair of bone, cartilage and ligament tissue is of new interest. and for controlled drug release. The uses of sericin in the medical field are more recent but still promising too.

In the cosmetic field, on the other hand, the applications are still relatively limited; in fact, silk proteins have been recognized to possess, at least on a theoretical level, some properties that could be useful in the cosmetic field, but not capable of developing products of commercial interest; the silk proteins tested have in fact always shown modest degrees of activity.

The purpose of the invention is to provide a dermatological and / or cosmetic preparation having antityrosinase, antioxidant, anti-elastase and anti-collagenase activity.

The present invention therefore relates to a dermatological and / or cosmetic preparation with anti-tyrosinase, antioxidant, anti-elastase and anti-collagenase activity, comprising at least one silk protein (fibroin, sericin or their mixtures) extracted from cocoons of silkworms (Bombyx mori) of a colored cocoon breed.

The silk proteins used in the cosmetic and / or dermatological preparations according to the invention are obtained from the cocoons of silkworms of particular relatively little diffused breeds, namely colored cocoon breeds, in particular non-hybrid breeds; in fact, it has been recognized that the silk proteins of these breeds have antityrosinase, antioxidant, anti-elastase and anti-collagenase activity superior to other breeds and in particular to the common (normally hybrid) breeds with white cocoons.

Particularly effective effects have been observed with silk proteins obtained from silkworm cocoons of the Nistari breed.

Also particularly effective were the silk proteins extracted from cocoons of silkworms of other races (non-hybrid) with colored cocoons; specifically, the following breeds were found to be particularly effective and therefore usable according to the invention: ADPR, AP Alpe, Nistari modified, Verde Ovale.

Experimental studies have in fact confirmed that the anti-tyrosinase and anti-elastase and anti-collagenase activities of sericin and fibroin produced by silkworms (Bombyx mori) of different races show significant differences.

Precisely, silk proteins extracted from the cocoons of the races identified by the present invention, that is to say, colored cocoon races (in particular: Nistari, ADPR, AP Alpe, Nistari modified, Verde Ovale) show higher activities than the proteins obtained from traditional breeds ( breeds with white cocoon) and are therefore more suitable for use in the dermatological and / or cosmetic field.

Preliminary studies indicate that also the anti-collagenase activity, as well as the anti-tyrosinase and anti-elastase antioxidant activities, is greater for the proteins deriving from the cocoons of the colored cocoon breeds, and in particular of the Nistari, ADPR, AP Alpe, modified Nistari breeds. Oval Green.

Both silk proteins (sericin and fibroin) have been shown to be effective for use in dermatological and / or cosmetic preparations with anti-tyrosinase action (skin lightening effect), anti-elastase and anti-collagenase antioxidant and therefore, in general, in anti-aging products and / or brighteners.

It was also found that the diet has a significant effect on the biological activity of both silk proteins; in particular, it has been observed that feeding the silkworms with mulberry leaves (natural food) increases the antithyrosinase activity of sericin and the antioxidant activity of fibroin compared to silkworms fed with artificial diet.

Therefore, the preparations according to the invention preferably comprise sericin and / or fibroin extracted from silkworm cocoons fed exclusively or at least partially with a natural diet and specifically with mulberry leaves.

The main data confirming the efficacy of silk proteins in the preparations according to the invention are summarized in the following tables.

Table 1 shows the results relating to the antithyrosinase activity of sericin, evaluated with the colorimetric method; the results are expressed in terms of absorbance (mean and standard error of the spectrophotometric readings) measured on:

- samples of sericin extracted from the cocoons of a colored cocoon race (Nistari) according to the invention;

- samples of sericin extracted from the cocoons of a reference breed with white cocoon (Orgosolo);

- positive control (Arbutin), which is known for its high antithyrosinase activity;

- negative control (reaction mixture without sericin sample).

It is evident from the reported data that the sericin obtained from the Nistari breed has antithyrosinase activity very close to Arbutin (positive control) and significantly higher than the reference breed (Orgosolo).

TABLE 1

Sample Number of samples Mean ± standard error Arbutin 150 0.029 ± 0.028

Nistari 314 0.092 ± 0.020

Orgosolo 248 0.478 ± 0.022

Negative control 633 0.641 ± 0.014

Table 2 shows the results relating to the antioxidant activity of sericin, evaluated with the colorimetric method; table 2 shows the sample size and the average values of the percentage antioxidant activity with relative standard error for:

- samples of sericin extracted from cocoons of the Nistari breed (invention);

- samples of sericin extracted from cocoons of the Orgosolo breed (reference breed with white cocoon);

- positive control (ascorbic acid).

It is evident from the data reported that the sericin obtained from the Nistari breed has drastically superior antioxidant activity to the reference breed.

TABLE 2

Sample Number of samples Mean ± standard error Ascorbic acid 11 96.716 ± 0.675 Nistari 15 74.911 ± 2.461 Orgosol 12 29.338 ± 2.556

Table 3 reports the results relating to the antithyrosinase activity of fibroin, similarly to Table 1 and with the same modalities.

Here too it is evident that the Nistari breed has significantly higher antithyrosinase activity than the reference breed.

TABLE 3

Sample Number of samples Mean ± standard error Arbutin 132 0.026 ± 0.010

Nistari 198 0.231 ± 0.009

Orgosolo 396 0.306 ± 0.007

Negative Control 481 0.468 ± 0.013

Table 4 is the analogue of table 2 for the antioxidant activity of fibroin.

It is evident from the data reported that the fibroin obtained from the Nistari breed has an antioxidant activity that is drastically superior to the reference breed.

TABLE 4

Sample Number of samples Mean ± standard error Ascorbic acid 8 96.5 ± 1.9

Nistari 12 14.2 ± 1.6

Orgosolo 24 7.8 ± 1.1

Combining the results obtained, it appears that the Nistari breed possesses greater antityrosinase and antioxidant-anti-elastase activities than the reference breeds. Consequently, a high anticollagenase activity is expected, confirmed by preliminary results.

Further tests, conducted on other colored cocoon breeds (ADPR, AP Alpe, modified Nistari, Verde Ovale) confirm the superior anti-tyrosinase, anti-elastase and anti-collagenase antioxidant activity of sericin and fibroin obtained from these breeds compared to the reference white cocoon breeds.

Further studies confirmed the effect of the diet on the antithyrosinase and antioxidant activity of fibroin and / or sericin.

Worms fed with a natural diet, in particular with mulberry leaves, in fact produced proteins with greater antithyrosinase and / or antioxidant activity compared to silkworms of the same breed fed with an artificial diet.

The most significant increases were observed in the antithyrosinase activity of sericin and in the antioxidant activity of fibroin, comparing the proteins extracted from cocoons of silkworms fed with mulberry leaves compared to silkworms fed with artificial foods.

Lower effects were instead observed in the antioxidant activity of sericin and in the antithyrosinase activity of fibroin.

Ultimately, fibroin and sericin extracted from silkworm cocoons of the breeds identified in accordance with the invention, that is, non-hybrid colored cocoon breeds, can be used to make dermatological and / or cosmetic preparations, having antithyrosinase, antioxidant activity , anti-elastase and / or anti-collagenase.

Clearly, various other ingredients, known and / or commonly used in the field, can also be included in these preparations, where necessary.

Finally, it is understood that further modifications and variations may be made to the dermatological and / or cosmetic preparations described herein which do not depart from the scope of the attached claims.

Claims (5)

CLAIMS 1. Dermatological and / or cosmetic preparation with anti-tyrosinase, antioxidant, anti-elastase and / or anti-collagenase activity, comprising at least one silk protein extracted from silkworm cocoons (Bombyx mori) of a colored cocoon race. 2. Preparation according to claim 1, comprising fibroin and / or sericin. 3. Preparation according to claim 1 or 2, wherein the silk protein is extracted from cocoons of silkworms of one or more races selected from the group consisting of: Nistari, ADPR, AP Alpe, modified Nistari, Verde Ovale. 4. Preparation according to one of the preceding claims, wherein the silk protein is extracted from silkworm cocoons of the Nistari breed. Preparation according to one of the preceding claims, wherein the silk protein is extracted from silkworm cocoons fed with a natural diet and specifically mulberry leaves.