IT201700087291A1 - Metodo di imaging di un campione biologico e relativa sonda - Google Patents

Metodo di imaging di un campione biologico e relativa sonda

Info

Publication number
IT201700087291A1
IT201700087291A1 IT102017000087291A IT201700087291A IT201700087291A1 IT 201700087291 A1 IT201700087291 A1 IT 201700087291A1 IT 102017000087291 A IT102017000087291 A IT 102017000087291A IT 201700087291 A IT201700087291 A IT 201700087291A IT 201700087291 A1 IT201700087291 A1 IT 201700087291A1
Authority
IT
Italy
Prior art keywords
biological
imaging method
probe sample
relative probe
relative
Prior art date
Application number
IT102017000087291A
Other languages
English (en)
Inventor
Pier Paolo Pompa
Original Assignee
Fondazione St Italiano Tecnologia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fondazione St Italiano Tecnologia filed Critical Fondazione St Italiano Tecnologia
Priority to IT102017000087291A priority Critical patent/IT201700087291A1/it
Priority to CN201880055465.3A priority patent/CN111226115B/zh
Priority to JP2020504155A priority patent/JP7138160B2/ja
Priority to EP18759431.2A priority patent/EP3658912B1/en
Priority to PCT/IT2018/050142 priority patent/WO2019021336A1/en
Publication of IT201700087291A1 publication Critical patent/IT201700087291A1/it
Priority to US16/774,419 priority patent/US11300517B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2223/00—Investigating materials by wave or particle radiation
    • G01N2223/40—Imaging
    • G01N2223/418—Imaging electron microscope

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Nanotechnology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
IT102017000087291A 2017-07-28 2017-07-28 Metodo di imaging di un campione biologico e relativa sonda IT201700087291A1 (it)

Priority Applications (6)

Application Number Priority Date Filing Date Title
IT102017000087291A IT201700087291A1 (it) 2017-07-28 2017-07-28 Metodo di imaging di un campione biologico e relativa sonda
CN201880055465.3A CN111226115B (zh) 2017-07-28 2018-07-27 生物样品的成像方法和相应的探针
JP2020504155A JP7138160B2 (ja) 2017-07-28 2018-07-27 生体試料及び対応するプローブを画像化する方法
EP18759431.2A EP3658912B1 (en) 2017-07-28 2018-07-27 Method for imaging a biological sample and corresponding probe
PCT/IT2018/050142 WO2019021336A1 (en) 2017-07-28 2018-07-27 METHOD FOR IMAGING A BIOLOGICAL SAMPLE AND CORRESPONDING PROBE
US16/774,419 US11300517B2 (en) 2017-07-28 2020-01-28 Method for imaging a biological sample and corresponding probe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT102017000087291A IT201700087291A1 (it) 2017-07-28 2017-07-28 Metodo di imaging di un campione biologico e relativa sonda

Publications (1)

Publication Number Publication Date
IT201700087291A1 true IT201700087291A1 (it) 2019-01-28

Family

ID=60991001

Family Applications (1)

Application Number Title Priority Date Filing Date
IT102017000087291A IT201700087291A1 (it) 2017-07-28 2017-07-28 Metodo di imaging di un campione biologico e relativa sonda

Country Status (6)

Country Link
US (1) US11300517B2 (it)
EP (1) EP3658912B1 (it)
JP (1) JP7138160B2 (it)
CN (1) CN111226115B (it)
IT (1) IT201700087291A1 (it)
WO (1) WO2019021336A1 (it)

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5384265A (en) * 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US7892781B2 (en) * 2001-03-30 2011-02-22 Nanoprobes, Inc. Detecting a target using a composite probe comprising a directing agent, a metal nanoparticle and an enzyme
EP1801593A1 (en) 2005-12-22 2007-06-27 Koninklijke Philips Electronics N.V. A method of imaging biological specimens using inorganic nanoparticles as label agents
JP5543888B2 (ja) * 2010-09-29 2014-07-09 富士フイルム株式会社 イムノクロマトグラフ検査方法および装置
IL281714B2 (en) * 2011-04-01 2023-11-01 Immunogen Inc Methods for increasing the effectiveness of FOLR1 cancer therapy
CN104849449B (zh) * 2015-05-13 2017-04-05 东南大学 酶标抗体‑金纳米探针在二氨基联苯胺催化显色及暗场成像的应用
CN106111131A (zh) * 2016-06-24 2016-11-16 许昌学院 一种树枝状金铂合金纳米颗粒模拟酶及其制备方法和应用
CN106236740A (zh) * 2016-07-14 2016-12-21 中国人民解放军第四军医大学 一种改变trpc6在催产素神经元中表达的方法及其应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DEBORAH PEDONE ET AL: "Platinum nanoparticles in nanobiomedicine", CHEMICAL SOCIETY REVIEWS, vol. 46, no. 16, 11 July 2017 (2017-07-11), pages 4951 - 4975, XP055452309, ISSN: 0306-0012, DOI: 10.1039/C7CS00152E *
ISABELL BEGEMANN ET AL: "Correlative Light Electron Microscopy: Connecting Synaptic Structure and Function", FRONTIERS IN SYNAPTIC NEUROSCIENCE, vol. 8, 23 August 2016 (2016-08-23), pages 28, XP055452310, DOI: 10.3389/fnsyn.2016.00028 *
VIKTORIA LISS ET AL: "Self-labelling enzymes as universal tags for fluorescence microscopy, super-resolution microscopy and electron microscopy", SCIENTIFIC REPORTS, vol. 5, no. 1, 8 December 2015 (2015-12-08), XP055397564, DOI: 10.1038/srep17740 *

Also Published As

Publication number Publication date
US20200166463A1 (en) 2020-05-28
US11300517B2 (en) 2022-04-12
JP2020528557A (ja) 2020-09-24
EP3658912A1 (en) 2020-06-03
CN111226115A (zh) 2020-06-02
WO2019021336A1 (en) 2019-01-31
CN111226115B (zh) 2023-10-31
EP3658912B1 (en) 2022-05-11
JP7138160B2 (ja) 2022-09-15

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