IT1396725B1 - METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM - Google Patents

METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM

Info

Publication number
IT1396725B1
IT1396725B1 ITRM2009A000595A ITRM20090595A IT1396725B1 IT 1396725 B1 IT1396725 B1 IT 1396725B1 IT RM2009A000595 A ITRM2009A000595 A IT RM2009A000595A IT RM20090595 A ITRM20090595 A IT RM20090595A IT 1396725 B1 IT1396725 B1 IT 1396725B1
Authority
IT
Italy
Prior art keywords
functionality
extend
improve
vitro sperm
sperm
Prior art date
Application number
ITRM2009A000595A
Other languages
Italian (it)
Inventor
Riccardo Calafiore
Massimo Menegazzo
Daniela Zuccarello
Giovanni Luca
Carlo Foresta
Andrea Garolla
Francesca Mancuso
Ennio Becchetti
Mario Calvitti
Toni Luca De
Alberto Ferlin
Claudio Nastruzzi
Original Assignee
Università degli Studi di Perugia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Università degli Studi di Perugia filed Critical Università degli Studi di Perugia
Priority to ITRM2009A000595A priority Critical patent/IT1396725B1/en
Priority to PCT/IB2010/055190 priority patent/WO2011061677A1/en
Publication of ITRM20090595A1 publication Critical patent/ITRM20090595A1/en
Application granted granted Critical
Publication of IT1396725B1 publication Critical patent/IT1396725B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0683—Cells of the male genital tract, e.g. prostate, epididymis; Non-germinal cells from testis, e.g. Leydig cells, Sertoli cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/24—Genital tract cells, non-germinal cells from gonads
    • C12N2502/246—Cells of the male genital tract, non-germinal testis cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Reproductive Health (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
ITRM2009A000595A 2009-11-17 2009-11-17 METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM IT1396725B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ITRM2009A000595A IT1396725B1 (en) 2009-11-17 2009-11-17 METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM
PCT/IB2010/055190 WO2011061677A1 (en) 2009-11-17 2010-11-16 Method to prolong and improve the functionality of spermatozoa in vitro

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITRM2009A000595A IT1396725B1 (en) 2009-11-17 2009-11-17 METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM

Publications (2)

Publication Number Publication Date
ITRM20090595A1 ITRM20090595A1 (en) 2011-05-18
IT1396725B1 true IT1396725B1 (en) 2012-12-14

Family

ID=42115656

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM2009A000595A IT1396725B1 (en) 2009-11-17 2009-11-17 METHOD TO EXTEND AND IMPROVE THE FUNCTIONALITY OF VITRO SPERM

Country Status (2)

Country Link
IT (1) IT1396725B1 (en)
WO (1) WO2011061677A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106906177B (en) * 2017-01-06 2021-06-22 中国人民解放军第二军医大学 Naked mole rat testicular interstitial cell separation, purification and culture method
CN109797131A (en) * 2019-02-22 2019-05-24 青岛农业大学 A kind of preparation method improving herd boar sperm motility agonist

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002339299A1 (en) * 2001-05-18 2002-12-03 Cornell Research Foundation, Inc. In vitro production of haploid germ cells
KR100569168B1 (en) * 2003-08-08 2006-04-07 (주)아비코아생명공학연구소 Method for Culturing Avian Spermatogonial Stem Cells and Avian Spermatogonial Stem Cells Prepared thereby
WO2007089572A2 (en) * 2006-01-26 2007-08-09 John Constance M Proliferative primary human sertoli cell cultures and their applications

Also Published As

Publication number Publication date
ITRM20090595A1 (en) 2011-05-18
WO2011061677A1 (en) 2011-05-26

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