IT1256818B - Metodo di rivelazione di effettori citotossici. - Google Patents

Metodo di rivelazione di effettori citotossici.

Info

Publication number
IT1256818B
IT1256818B ITRM920932A ITRM920932A IT1256818B IT 1256818 B IT1256818 B IT 1256818B IT RM920932 A ITRM920932 A IT RM920932A IT RM920932 A ITRM920932 A IT RM920932A IT 1256818 B IT1256818 B IT 1256818B
Authority
IT
Italy
Prior art keywords
detection method
cytotoxic
supernatant
target cells
effector
Prior art date
Application number
ITRM920932A
Other languages
English (en)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Priority to ITRM920932A priority Critical patent/IT1256818B/it
Publication of ITRM920932A0 publication Critical patent/ITRM920932A0/it
Priority to EP93830522A priority patent/EP0605370A1/en
Priority to CA002112306A priority patent/CA2112306A1/en
Publication of ITRM920932A1 publication Critical patent/ITRM920932A1/it
Application granted granted Critical
Publication of IT1256818B publication Critical patent/IT1256818B/it

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Metodo di rivelazione di effettori citotossici comprendente le seguenti fasi: - incubare cellule bersaglio in una soluzione comprendente detto effettore; - separare il sovranatante dalla miscela di incubazione; - rivelare e mezzo di reazione enzimatica la presenza di almeno un enzima microsomiale in detto sovranatante; - determinare il rilascio sperimentale (ER) di detto enzima microsomiale sottraendo dal valore ottenuto un valore di controllo, ottenuto esponendo dette cellule bersaglio ad una soluzione mancante di detto effettore citotossico.
ITRM920932A 1992-12-28 1992-12-28 Metodo di rivelazione di effettori citotossici. IT1256818B (it)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ITRM920932A IT1256818B (it) 1992-12-28 1992-12-28 Metodo di rivelazione di effettori citotossici.
EP93830522A EP0605370A1 (en) 1992-12-28 1993-12-23 Method to detect cytotoxic effectors
CA002112306A CA2112306A1 (en) 1992-12-28 1993-12-23 Method to detect cytotoxic effectors

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITRM920932A IT1256818B (it) 1992-12-28 1992-12-28 Metodo di rivelazione di effettori citotossici.

Publications (3)

Publication Number Publication Date
ITRM920932A0 ITRM920932A0 (it) 1992-12-28
ITRM920932A1 ITRM920932A1 (it) 1994-06-28
IT1256818B true IT1256818B (it) 1995-12-21

Family

ID=11401359

Family Applications (1)

Application Number Title Priority Date Filing Date
ITRM920932A IT1256818B (it) 1992-12-28 1992-12-28 Metodo di rivelazione di effettori citotossici.

Country Status (3)

Country Link
EP (1) EP0605370A1 (it)
CA (1) CA2112306A1 (it)
IT (1) IT1256818B (it)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2755977B1 (fr) * 1996-11-20 1998-12-24 Pasteur Merieux Serums Vacc Methode de mise en evidence de l'activite cytotoxique de cellules

Also Published As

Publication number Publication date
ITRM920932A0 (it) 1992-12-28
ITRM920932A1 (it) 1994-06-28
CA2112306A1 (en) 1994-06-29
EP0605370A1 (en) 1994-07-06

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19961129