IT1256385B - AUTOMATIC ELECTROPHORESIS EQUIPMENT - Google Patents

AUTOMATIC ELECTROPHORESIS EQUIPMENT

Info

Publication number
IT1256385B
IT1256385B ITMI922602A ITMI922602A IT1256385B IT 1256385 B IT1256385 B IT 1256385B IT MI922602 A ITMI922602 A IT MI922602A IT MI922602 A ITMI922602 A IT MI922602A IT 1256385 B IT1256385 B IT 1256385B
Authority
IT
Italy
Prior art keywords
substrate
sheet
unit
strip
cutter
Prior art date
Application number
ITMI922602A
Other languages
Italian (it)
Inventor
Hideo Suzuki
Original Assignee
Olympus Optical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP3300783A external-priority patent/JP3071908B2/en
Priority claimed from JP01216792A external-priority patent/JP3159499B2/en
Application filed by Olympus Optical Co filed Critical Olympus Optical Co
Publication of ITMI922602A0 publication Critical patent/ITMI922602A0/en
Publication of ITMI922602A1 publication Critical patent/ITMI922602A1/en
Application granted granted Critical
Publication of IT1256385B publication Critical patent/IT1256385B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44782—Apparatus specially adapted therefor of a plurality of samples

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Apparecchiatura automatica (10) di elettroforesi avente un'unità (13) a rulli di alimentazione per alimentare una striscia (12) di substrato di lunghezza prefissata ed una taglierina (15) per tagliare la striscia (12) in un foglio (17) di substrato di lunghezza prefissata. Un'unità (16) di controllo controlla un motore (14) a passo per l'azionamento dell'unità (13) a rulli e della taglierina (15) in base ad un numero immesso volontariamente x di campioni da applicare al foglio (17) di substrato. In tal modo, la striscia (12) di substrasto viene tagliata ad una lunghezza corrispondente al numero x.In un'unità umettatrice intermedia (19), il foglio (17) di substrato viene umettato con una soluzione tampone (18). Quindi, in un'unità (23) applicatrice di campione, campioni vengono applicati alla superficie del foglio (17) di substrato. Susseguentemente, in un'unità (28) di elettroforesi, al foglio (17) di substrato viene applicata una corrente elettrica. In un'unità (34) di post-trattamento il foglio (17) viene sottoposto in successione a trattamenti di tintura, decolorazione ed essiccazione ottenendo così un'immagine elettroforetica. L'immagine elettroforetica viene sottoposta a misurazione fotometrica mediante un densitometro (35). Come risultato il costo dell'analisi per campione viene ridotto, mentre viene abbreviato il tempo di analisi.Automatic electrophoresis apparatus (10) having a feed roller unit (13) for feeding a strip (12) of substrate of predetermined length and a cutter (15) for cutting the strip (12) into a sheet (17) of substrate of predetermined length. A control unit (16) controls a stepper motor (14) to drive the roller unit (13) and the cutter (15) based on a voluntarily entered number x of samples to be applied to the sheet (17 ) of substrate. In this way, the substrate strip (12) is cut to a length corresponding to the number x. In an intermediate wetting unit (19), the substrate sheet (17) is moistened with a buffer solution (18). Then, in a sample applicator unit (23), samples are applied to the surface of the substrate sheet (17). Subsequently, in an electrophoresis unit (28), an electric current is applied to the substrate sheet (17). In a post-treatment unit (34) the sheet (17) is subjected to dyeing, bleaching and drying treatments in succession thus obtaining an electrophoretic image. The electrophoretic image is subjected to photometric measurement by means of a densitometer (35). As a result the cost of the analysis per sample is reduced, while the analysis time is shortened.

ITMI922602A 1991-11-15 1992-11-13 AUTOMATIC ELECTROPHORESIS EQUIPMENT IT1256385B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP3300783A JP3071908B2 (en) 1991-11-15 1991-11-15 Automatic electrophoresis device
JP01216792A JP3159499B2 (en) 1992-01-27 1992-01-27 Automatic electrophoresis device

Publications (3)

Publication Number Publication Date
ITMI922602A0 ITMI922602A0 (en) 1992-11-13
ITMI922602A1 ITMI922602A1 (en) 1994-05-13
IT1256385B true IT1256385B (en) 1995-12-04

Family

ID=26347737

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI922602A IT1256385B (en) 1991-11-15 1992-11-13 AUTOMATIC ELECTROPHORESIS EQUIPMENT

Country Status (2)

Country Link
DE (1) DE4238452C2 (en)
IT (1) IT1256385B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0694676A (en) * 1992-09-10 1994-04-08 Olympus Optical Co Ltd Automatic electrophoretic device
JP3349752B2 (en) * 1993-03-31 2002-11-25 オリンパス光学工業株式会社 Automatic electrophoresis device
IT1269328B (en) * 1994-04-18 1997-03-26 Helena Laboratoires Italia Spa AUTOMATIC EQUIPMENT FOR ELECTROPHORETIC, ELECTROCHEMICAL AND CHEMICAL ANALYSIS

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4360418A (en) * 1981-06-12 1982-11-23 Helena Laboratories Corporation Electrophoresis and staining apparatus

Also Published As

Publication number Publication date
ITMI922602A1 (en) 1994-05-13
ITMI922602A0 (en) 1992-11-13
DE4238452A1 (en) 1993-05-19
DE4238452C2 (en) 1994-10-27

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19971129