IT1254316B - Procedimento per la preparazione di acilfosfatasi mediante cellule geneticamente modificate, e vettori ricombinanti ed organismi modificati ottenibili nel procedimento. - Google Patents
Procedimento per la preparazione di acilfosfatasi mediante cellule geneticamente modificate, e vettori ricombinanti ed organismi modificati ottenibili nel procedimento.Info
- Publication number
- IT1254316B IT1254316B ITRM920232A ITRM920232A IT1254316B IT 1254316 B IT1254316 B IT 1254316B IT RM920232 A ITRM920232 A IT RM920232A IT RM920232 A ITRM920232 A IT RM920232A IT 1254316 B IT1254316 B IT 1254316B
- Authority
- IT
- Italy
- Prior art keywords
- genetically modified
- possibly
- acyl phosphatase
- acyl
- phosphatase
- Prior art date
Links
- 102000005991 Acylphosphatase Human genes 0.000 title abstract 6
- 108700006311 Acylphosphatases Proteins 0.000 title abstract 6
- 239000013598 vector Substances 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 108020004414 DNA Proteins 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 3
- 239000012634 fragment Substances 0.000 abstract 3
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 210000001723 extracellular space Anatomy 0.000 abstract 2
- 210000003093 intracellular space Anatomy 0.000 abstract 2
- 210000001322 periplasm Anatomy 0.000 abstract 2
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 239000000470 constituent Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000001939 inductive effect Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
- 239000013600 plasmid vector Substances 0.000 abstract 1
- 108091033319 polynucleotide Proteins 0.000 abstract 1
- 239000002157 polynucleotide Substances 0.000 abstract 1
- 102000040430 polynucleotide Human genes 0.000 abstract 1
- 108091008146 restriction endonucleases Proteins 0.000 abstract 1
- 230000003248 secreting effect Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Procedimento, per la produzione di acilfosfatasi mediante cellule geneticamente modificate, comprendente essenzialmente le seguenti operazioni:- digerire un vettore plasmidico (ad esempio il plasmide batterico pT7.7, la cui mappa genica è mostrata in figura 1) con endonucleasi di restrizione per ottenere un vettore a DNA lineare;- legare il vettore a DNA lineare così ottenuto ad un frammento polinucleotidico che codifica per la sintesi di acilfosfatasi, essendo le estremità di detto frammento compatibili con i siti di restrizione del suddetto vettore a DNA lineare;- clonare ed esprimere il detto frammento nucleotidico, eventualmente come proteina di fusione, in cellule geneticamente come proteina di fusione, in cellule geneticamente modificate in quanto includono detto DNA ricombinante, sotto il controllo di una sequenza promotore inducibile oppure costitutiva, essendo le cellule geneticamente modificate in grado di sintetizzare la acilfosfatasi nello spazio intracellulare ed, eventualmente, di secernerla nello spazio extracellulare oppure periplasmatico;- recuperare la acilfosfatasi dallo spazio intracellulare, dallo spazio extracellulare oppure dallo spazio periplasmatico eventualmente come proteina chimerica; e - eventualmente, scindere la proteina chimerica per ottenere acilfosfatasi tal quale.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITRM920232A IT1254316B (it) | 1992-04-01 | 1992-04-01 | Procedimento per la preparazione di acilfosfatasi mediante cellule geneticamente modificate, e vettori ricombinanti ed organismi modificati ottenibili nel procedimento. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ITRM920232A IT1254316B (it) | 1992-04-01 | 1992-04-01 | Procedimento per la preparazione di acilfosfatasi mediante cellule geneticamente modificate, e vettori ricombinanti ed organismi modificati ottenibili nel procedimento. |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| ITRM920232A0 ITRM920232A0 (it) | 1992-04-01 |
| ITRM920232A1 ITRM920232A1 (it) | 1993-10-01 |
| IT1254316B true IT1254316B (it) | 1995-09-14 |
Family
ID=11400898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ITRM920232A IT1254316B (it) | 1992-04-01 | 1992-04-01 | Procedimento per la preparazione di acilfosfatasi mediante cellule geneticamente modificate, e vettori ricombinanti ed organismi modificati ottenibili nel procedimento. |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1254316B (it) |
-
1992
- 1992-04-01 IT ITRM920232A patent/IT1254316B/it active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| ITRM920232A1 (it) | 1993-10-01 |
| ITRM920232A0 (it) | 1992-04-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted | ||
| TA | Fee payment date (situation as of event date), data collected since 19931001 |
Effective date: 19970422 |