HUT34237A - Process for producing transposone-carrier bifunctional recombinant dna cloning vector and transformants of the vector
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Process for producing transposone-carrier bifunctional recombinant dna cloning vector and transformants of the vector
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Publication number
HUT34237A
HUT34237AHU246883AHU246883AHUT34237AHU T34237 AHUT34237 AHU T34237AHU 246883 AHU246883 AHU 246883AHU 246883 AHU246883 AHU 246883AHU T34237 AHUT34237 AHU T34237A
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Application filed by Gyogyszerkutato IntezetfiledCriticalGyogyszerkutato Intezet
Priority to HU246883ApriorityCriticalpatent/HU197045B/en
Publication of HUT34237ApublicationCriticalpatent/HUT34237A/en
Publication of HU197045BpublicationCriticalpatent/HU197045B/en
Novel bifunctional recombinant DNA cloning vectors and vector transformants opt. contg. transposon and antibiotic-resistance determining genes are prepd. by coupling DNA fragments carrying antibiotic-resistance determining genes contg. functional replicant oligo in E. Coli cells and DNA fragments contg. functional replicant oligo in Streptomyces cells and opt. transposon DNA, transforming Streptomyces and E. Coli cells with the resulting recombinant vector DNA, selecting the transformants and opt. isolating the mutants.
HU246883A1983-07-111983-07-11Process for producing vectors cloning bifunctional recombinant dna and transformants of the vectors
HU197045B
(en)
Bacillus cloning vectors,recombinant dna molecules,bacillus hosts transformed with them and methods for expressing foreign dna sequences and producing polypeptides coded thereby