IT1236225B - Method for the determination of spermine in human serum and other biological liquids - Google Patents
Method for the determination of spermine in human serum and other biological liquidsInfo
- Publication number
- IT1236225B IT1236225B IT00957389A IT957389A IT1236225B IT 1236225 B IT1236225 B IT 1236225B IT 00957389 A IT00957389 A IT 00957389A IT 957389 A IT957389 A IT 957389A IT 1236225 B IT1236225 B IT 1236225B
- Authority
- IT
- Italy
- Prior art keywords
- spermine
- following
- standard
- test
- human serum
- Prior art date
Links
- PFNFFQXMRSDOHW-UHFFFAOYSA-N spermine Chemical compound NCCCNCCCCNCCCN PFNFFQXMRSDOHW-UHFFFAOYSA-N 0.000 title abstract 14
- 229940063675 spermine Drugs 0.000 title abstract 7
- 239000007788 liquid Substances 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 210000002966 serum Anatomy 0.000 title abstract 2
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 abstract 4
- 230000002255 enzymatic effect Effects 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 2
- 230000003287 optical effect Effects 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 1
- 102100032800 Spermine oxidase Human genes 0.000 abstract 1
- 108030006620 Spermine oxidases Proteins 0.000 abstract 1
- 230000004913 activation Effects 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 239000001166 ammonium sulphate Substances 0.000 abstract 1
- 239000012888 bovine serum Substances 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- AZQWKYJCGOJGHM-UHFFFAOYSA-N para-benzoquinone Natural products O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 238000001556 precipitation Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000011347 resin Substances 0.000 abstract 1
- 229920005989 resin Polymers 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
To determine the spermine in human serum and other biological liquids a clinical chemistry test has been created, which uses a concentrate enriched in spermine oxidases, partially purified of the other proteins present in the bovine serum, by means of precipitation with ammonium sulphate, and successive passage in ionic exchange resin (Enzyme E). The test permits the activation of the following reactionSperm. ox.Spermine + H2O - Oxidized Spermine + H2OPODH2O2 + phenol + 4-aminoantipyrine - quinone + H2Owith the following enzymatic mixturePeroxidases - 30 μl - (1mg/ml)4-amino antipyrine - 12 mgPhenol - 19.2 mgEnzyme E - 700 μlPhosphate Buffer0.1 pH 7.4 - 8.1 mlThe said enzymatic mixture may also be diluted, using the ratio, 1:5, with phosphate buffer 0.1M pH 7.4, and used according to the following methodology:Enzymatic mixture - 700 μlSample or standard (spermine) - 70 μlIncubation time 20' at 37 degrees CReading at 510nm against white reactives.The concentration of spermine is determined by the following formula:Spermine conc. = Optical density test x standard conc. / Standard optical density
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT00957389A IT1236225B (en) | 1989-11-30 | 1989-11-30 | Method for the determination of spermine in human serum and other biological liquids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT00957389A IT1236225B (en) | 1989-11-30 | 1989-11-30 | Method for the determination of spermine in human serum and other biological liquids |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT8909573A0 IT8909573A0 (en) | 1989-11-30 |
| IT1236225B true IT1236225B (en) | 1993-01-25 |
Family
ID=11132366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT00957389A IT1236225B (en) | 1989-11-30 | 1989-11-30 | Method for the determination of spermine in human serum and other biological liquids |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1236225B (en) |
-
1989
- 1989-11-30 IT IT00957389A patent/IT1236225B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| IT8909573A0 (en) | 1989-11-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Järvinen | Purification and some characteristics of the human epidermal SH-protease inhibitor | |
| Kosaka et al. | Chemically defined neuron groups and their subpopulations in the glomerular layer of the rat main olfactory bulb | |
| ATE221918T1 (en) | COMPOSITIONS AND METHODS FOR STRUCTURAL PROTEIN DETERMINATION | |
| ATE117437T1 (en) | TEST OF BODY FLUID TO MEASURE BONE RESORPTION. | |
| NO930770L (en) | IMMUNOLOGICAL PROCEDURE FOR DETERMINING A HEMOGLOBIN DERIVATIVE | |
| ZA891568B (en) | Method for determination of a polyvalent substance using an immunoaggregate | |
| Kosakowski et al. | The Subunit Structure of Phenylalanyl‐tRNA Synthetase of Escherichia coli | |
| CA2140794A1 (en) | Assay for the determination of protein in a biological sample | |
| ES485763A1 (en) | Method and composition for direct determination of iron in blood serum | |
| Barrett | Human cathepsin D | |
| DE3273606D1 (en) | Process for the purification or concentration of biologically active proteins, and appropriate means therefor | |
| IT1236225B (en) | Method for the determination of spermine in human serum and other biological liquids | |
| Obinata et al. | The subunit structure of myosin from skeletal muscle of the early chick embryo | |
| ATE137864T1 (en) | IMMUNODETERMINATION OF HUMAN OSTEOCALCIN, REAGENT AND KIT THEREOF | |
| Lustberg et al. | The preparation and identification of various adducts of oxidized homogentisic acid and the development of a new sensitive colorimetric assay for homogentisic acid | |
| WO1999061468A3 (en) | The induction of antibiotic peptides by lait (scd14) protein | |
| Cukor et al. | Biological activity of heated diphtheria toxin | |
| DE69924719D1 (en) | PROCESS FOR THE ISOLATION AND MODIFICATION OF MOLECEPROTEINS | |
| ATE78342T1 (en) | METHODS OF DETECTING PREGNANCY DISORDERS. | |
| EP0343592A3 (en) | Method and reagent for the determination of iron | |
| Monder et al. | Studies on the non-enzymic conversion of dopa to melanin. III. The inhibition and mechanism of inhibition of dopa autoxidation by human blood plasma | |
| GR3017345T3 (en) | Diagnostic composition for rheumatoid arthritis. | |
| GB2011070A (en) | Assay of vitamin B12 | |
| Kakei et al. | Demonstration of cytoplasmic structural proteins in developing enamel matrix of the rat incisor | |
| Sil et al. | Comparative analysis of the membrane proteins and their specificities in neurons, protoplasmic astrocytes, and oligodendrocytes from rat brain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 0001 | Granted |