IT1222604B - Compsn. for assaying magnesium in aq. fluid, esp. serum - Google Patents
Compsn. for assaying magnesium in aq. fluid, esp. serumInfo
- Publication number
- IT1222604B IT1222604B IT21814/87A IT2181487A IT1222604B IT 1222604 B IT1222604 B IT 1222604B IT 21814/87 A IT21814/87 A IT 21814/87A IT 2181487 A IT2181487 A IT 2181487A IT 1222604 B IT1222604 B IT 1222604B
- Authority
- IT
- Italy
- Prior art keywords
- iii
- compsn
- glycerol
- cosubstrate
- peroxidase
- Prior art date
Links
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 title 1
- 239000012530 fluid Substances 0.000 title 1
- 229910052749 magnesium Inorganic materials 0.000 title 1
- 239000011777 magnesium Substances 0.000 title 1
- 210000002966 serum Anatomy 0.000 title 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 abstract 6
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 abstract 4
- 102000057621 Glycerol kinases Human genes 0.000 abstract 3
- 108700016170 Glycerol kinases Proteins 0.000 abstract 3
- PLIKAWJENQZMHA-UHFFFAOYSA-N 4-aminophenol Chemical compound NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 abstract 2
- CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 abstract 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical group Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 abstract 1
- LWKJNIMGNUTZOO-UHFFFAOYSA-N 3,5-dichloro-2-hydroxybenzenesulfonic acid Chemical compound OC1=C(Cl)C=C(Cl)C=C1S(O)(=O)=O LWKJNIMGNUTZOO-UHFFFAOYSA-N 0.000 abstract 1
- LSMMRJUHLKJNLR-UHFFFAOYSA-N 3-methyl-1,3-benzothiazol-2-one Chemical compound C1=CC=C2SC(=O)N(C)C2=C1 LSMMRJUHLKJNLR-UHFFFAOYSA-N 0.000 abstract 1
- 241000193792 Aerococcus viridans Species 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 241000593950 Streptomyces canus Species 0.000 abstract 1
- 229910052783 alkali metal Inorganic materials 0.000 abstract 1
- -1 alkali metal salt Chemical class 0.000 abstract 1
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Compsn for determining the Mg concn in an aq liq contains glycerol kinase (GK); alpha-glycerol phosphate oxidase (GP0); a cpd (I) with peroxidase-equivalent activity; ATP; glycerol; a peroxidase-oxidisable cosubstrate (II); an indicator-cosubstrate coupler (III); a buffer to maintain pH 5.5-9.2, and water. The buffer is Tris-HCl, and the compsn is formulated as 2 reagent solns; one contains glycerol and (III), the other contains all other components. (III) are benzidine, p-phenylenediamine or p-aminophenol cpds; 4-aminoantipyrine (AAP); 3-methyl-2-benzothiazolinone or their mixts, and (III) is at least one NH4 or alkali metal salt of 2-hydroxy-3,5-dichlorobenzene sulphonic acid (IIIa). GK is an Mg-dependent enzyme from Streptomyces canus or Bacillus stearothermophilus, and GP0 is derived from Aerococcus viridans.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT21814/87A IT1222604B (en) | 1987-09-07 | 1987-09-07 | Compsn. for assaying magnesium in aq. fluid, esp. serum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT21814/87A IT1222604B (en) | 1987-09-07 | 1987-09-07 | Compsn. for assaying magnesium in aq. fluid, esp. serum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT8721814A0 IT8721814A0 (en) | 1987-09-07 |
| IT1222604B true IT1222604B (en) | 1990-09-05 |
Family
ID=11187227
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT21814/87A IT1222604B (en) | 1987-09-07 | 1987-09-07 | Compsn. for assaying magnesium in aq. fluid, esp. serum |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1222604B (en) |
-
1987
- 1987-09-07 IT IT21814/87A patent/IT1222604B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| IT8721814A0 (en) | 1987-09-07 |
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