IT1207913B - Chromatographic and ion exchange column - Google Patents
Chromatographic and ion exchange columnInfo
- Publication number
- IT1207913B IT1207913B IT7941502A IT4150279A IT1207913B IT 1207913 B IT1207913 B IT 1207913B IT 7941502 A IT7941502 A IT 7941502A IT 4150279 A IT4150279 A IT 4150279A IT 1207913 B IT1207913 B IT 1207913B
- Authority
- IT
- Italy
- Prior art keywords
- acid sequence
- nucleic acid
- suspect
- reaction mixt
- hybridised
- Prior art date
Links
- 238000005342 ion exchange Methods 0.000 title 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 7
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 6
- 239000000523 sample Substances 0.000 abstract 5
- 238000006243 chemical reaction Methods 0.000 abstract 4
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 2
- 108020004682 Single-Stranded DNA Proteins 0.000 abstract 2
- 230000002068 genetic effect Effects 0.000 abstract 2
- 230000029936 alkylation Effects 0.000 abstract 1
- 238000005804 alkylation reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Abstract
A method for assaying for the presence or quantity of a suspect nucleic acid sequence of at least ten bases in a suspect sample contg. single stranded DNA or RNA, comprises: (a) contacting suspect sample with a molecular genetic probe comprising a modified nucleic acid sequence contg. complementary single stranded DNA or RNA hybridisable with the suspect nucleic acid sequence, to form a hybridised complex, said modified nucleic acid sequence having been formed by alkylation, sulphonation, nitrosonation, or nitrophenylation; (b) removing non-hybridised nucleic acids to form a first reaction mixt. consisting essentially of the hybridised complex; (c) contacting the first reaction mixt. with antibodies specific to the modified portion of the modified nucleic acid sequence of said molecular genetic probe to form an antibody bound complex; (d) adding a labelling agent adapted to signal the presence of said antibodies; and (e) removing unreacted labelled antibody to form a second reaction mixt. which is essentially free of unreacted labelled antibody; (f) measuring the second reaction mixt. for the presence and/or extent of the presence of the labelled antibodies as an indication of the extent or presence of the suspect nucleic acid sequence in the sample
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT7941502A IT1207913B (en) | 1979-01-11 | 1979-01-11 | Chromatographic and ion exchange column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT7941502A IT1207913B (en) | 1979-01-11 | 1979-01-11 | Chromatographic and ion exchange column |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IT7941502A0 IT7941502A0 (en) | 1979-01-11 |
| IT1207913B true IT1207913B (en) | 1989-06-01 |
Family
ID=11250298
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IT7941502A IT1207913B (en) | 1979-01-11 | 1979-01-11 | Chromatographic and ion exchange column |
Country Status (1)
| Country | Link |
|---|---|
| IT (1) | IT1207913B (en) |
-
1979
- 1979-01-11 IT IT7941502A patent/IT1207913B/en active
Also Published As
| Publication number | Publication date |
|---|---|
| IT7941502A0 (en) | 1979-01-11 |
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