IT1207575B - Smoking preventing compsn. - Google Patents

Smoking preventing compsn.

Info

Publication number
IT1207575B
IT1207575B IT8620806A IT2080686A IT1207575B IT 1207575 B IT1207575 B IT 1207575B IT 8620806 A IT8620806 A IT 8620806A IT 2080686 A IT2080686 A IT 2080686A IT 1207575 B IT1207575 B IT 1207575B
Authority
IT
Italy
Prior art keywords
acid sequence
nucleic acid
suspect
reaction mixt
hybridised
Prior art date
Application number
IT8620806A
Other languages
Italian (it)
Other versions
IT8620806A0 (en
Inventor
Mario Gini
Original Assignee
Gini Mario Dr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gini Mario Dr filed Critical Gini Mario Dr
Priority to IT8620806A priority Critical patent/IT1207575B/en
Publication of IT8620806A0 publication Critical patent/IT8620806A0/en
Application granted granted Critical
Publication of IT1207575B publication Critical patent/IT1207575B/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for assaying for the presence or quantity of a suspect nucleic acid sequence of at least ten bases in a suspect sample contg. single stranded DNA or RNA, comprises: (a) contacting suspect sample with a molecular genetic probe comprising a modified nucleic acid sequence contg. complementary single stranded DNA or RNA hybridisable with the suspect nucleic acid sequence, to form a hybridised complex, said modified nucleic acid sequence having been formed by alkylation, sulphonation, nitrosonation, or nitrophenylation; (b) removing non-hybridised nucleic acids to form a first reaction mixt. consisting essentially of the hybridised complex; (c) contacting the first reaction mixt. with antibodies specific to the modified portion of the modified nucleic acid sequence of said molecular genetic probe to form an antibody bound complex; (d) adding a labelling agent adapted to signal the presence of said antibodies; and (e) removing unreacted labelled antibody to form a second reaction mixt. which is essentially free of unreacted labelled antibody; (f) measuring the second reaction mixt. for the presence and/or extent of the presence of the labelled antibodies as an indication of the extent or presence of the suspect nucleic acid sequence in the sample
IT8620806A 1986-06-17 1986-06-17 Smoking preventing compsn. IT1207575B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
IT8620806A IT1207575B (en) 1986-06-17 1986-06-17 Smoking preventing compsn.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8620806A IT1207575B (en) 1986-06-17 1986-06-17 Smoking preventing compsn.

Publications (2)

Publication Number Publication Date
IT8620806A0 IT8620806A0 (en) 1986-06-17
IT1207575B true IT1207575B (en) 1989-05-25

Family

ID=11172333

Family Applications (1)

Application Number Title Priority Date Filing Date
IT8620806A IT1207575B (en) 1986-06-17 1986-06-17 Smoking preventing compsn.

Country Status (1)

Country Link
IT (1) IT1207575B (en)

Also Published As

Publication number Publication date
IT8620806A0 (en) 1986-06-17

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Legal Events

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TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970630