IN2014DN10904A - - Google Patents

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Publication number
IN2014DN10904A
IN2014DN10904A IN10904DEN2014A IN2014DN10904A IN 2014DN10904 A IN2014DN10904 A IN 2014DN10904A IN 10904DEN2014 A IN10904DEN2014 A IN 10904DEN2014A IN 2014DN10904 A IN2014DN10904 A IN 2014DN10904A
Authority
IN
India
Prior art keywords
viruses
lymphotropism
dna
pcr
lymphocyte
Prior art date
Application number
Inventor
Nariman Gulyamov
Original Assignee
Obschestvo S Ogranichennoi Otvetstvennostyu New Medical Technologies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Obschestvo S Ogranichennoi Otvetstvennostyu New Medical Technologies filed Critical Obschestvo S Ogranichennoi Otvetstvennostyu New Medical Technologies
Publication of IN2014DN10904A publication Critical patent/IN2014DN10904A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Use: medicine and biotechnology. Aim: to increase the reliability of determining infection by viruses with lymphotropism to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during EIA and PCR testing and to detect viruses with lymphotropism in biological material having a concentration of virus particles lower than the sensitivity threshold of EIA or PCR methods. Essence of the invention: the method for assessing the viability of viruses with lymphotropism comprises collecting biological material and determining whether said material contains virus RNA or DNA by means of conducting a polymerase chain reaction (PCR reaction). In addition a lymphocyte suspension is taken from the blood of healthy people to which lymphocytes an equal volume of biological material is added. This combination is then mixed incubated at a temperature of 37°? for a period of 6 8 hours and the lymphocytes are washed of plasma and broken down. The lymphocyte cytoplasm is then subjected to PCR testing. The detection of virus RNA or DNA in the lymphocyte cytoplasm indicates that the viruses have retained their viability. The absence of virus RNA or DNA in the lymphocyte cytoplasm indicates the inactivation of the viruses.
IN10904DEN2014 2012-06-18 2013-05-21 IN2014DN10904A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
UZIA20120233 2012-06-18
PCT/UZ2013/000001 WO2013192636A1 (en) 2012-06-18 2013-05-21 Method for assessing the viability of viruses with lymphotropism

Publications (1)

Publication Number Publication Date
IN2014DN10904A true IN2014DN10904A (en) 2015-09-11

Family

ID=84083119

Family Applications (1)

Application Number Title Priority Date Filing Date
IN10904DEN2014 IN2014DN10904A (en) 2012-06-18 2013-05-21

Country Status (10)

Country Link
US (7) US9879329B2 (en)
EP (3) EP3517628A1 (en)
KR (2) KR20160139059A (en)
CN (1) CN104066848A (en)
AP (1) AP2014008052A0 (en)
EA (1) EA032036B1 (en)
IL (1) IL235962B (en)
IN (1) IN2014DN10904A (en)
WO (1) WO2013192636A1 (en)
ZA (1) ZA201405954B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20160139059A (en) * 2012-06-18 2016-12-06 유한회사 뉴메디컬 테크놀로지스 Method for assessing the viability of viruses with lymphotropism

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674680A (en) * 1994-01-31 1997-10-07 The Rockefeller University Methods for the prognosis and monitoring of AIDS
US20090176202A1 (en) * 2007-03-23 2009-07-09 Rigel Pharmaceuticals, Inc. Methods of Detecting Inhibitors of VIF-Mediated APOBEC3G Degradation and HIV
KR20160139059A (en) * 2012-06-18 2016-12-06 유한회사 뉴메디컬 테크놀로지스 Method for assessing the viability of viruses with lymphotropism

Also Published As

Publication number Publication date
CN104066848A (en) 2014-09-24
US20180245167A1 (en) 2018-08-30
WO2013192636A1 (en) 2013-12-27
US20150125852A1 (en) 2015-05-07
US20170073779A1 (en) 2017-03-16
EA032036B1 (en) 2019-03-29
AP2014008052A0 (en) 2014-11-30
US20230078548A1 (en) 2023-03-16
EA201401097A1 (en) 2015-04-30
US10570466B2 (en) 2020-02-25
US10920289B2 (en) 2021-02-16
US11505837B2 (en) 2022-11-22
IL235962B (en) 2018-07-31
EP3517627A1 (en) 2019-07-31
US20210140001A1 (en) 2021-05-13
US20160369325A1 (en) 2016-12-22
US9957579B2 (en) 2018-05-01
EP2818558B1 (en) 2019-07-17
IL235962A0 (en) 2015-01-29
KR20140127864A (en) 2014-11-04
KR20160139059A (en) 2016-12-06
US20200190606A1 (en) 2020-06-18
EP2818558A1 (en) 2014-12-31
EP3517628A1 (en) 2019-07-31
US9879329B2 (en) 2018-01-30
EP2818558A4 (en) 2015-09-23
US9873922B2 (en) 2018-01-23
ZA201405954B (en) 2015-11-25

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