IN2014DN05656A - - Google Patents

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Publication number
IN2014DN05656A
IN2014DN05656A IN5656DEN2014A IN2014DN05656A IN 2014DN05656 A IN2014DN05656 A IN 2014DN05656A IN 5656DEN2014 A IN5656DEN2014 A IN 5656DEN2014A IN 2014DN05656 A IN2014DN05656 A IN 2014DN05656A
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IN
India
Prior art keywords
changed
mutant
protein
arginine
mutants
Prior art date
Application number
Inventor
Chil Yong Kang
Gyoung Nyoun Kim
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Univ Western Ontario
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Application filed by Univ Western Ontario filed Critical Univ Western Ontario
Publication of IN2014DN05656A publication Critical patent/IN2014DN05656A/en

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    • A61K39/205—Rhabdoviridae, e.g. rabies virus
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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Biomedical Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Hematology (AREA)
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  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Abstract

The present invention relates to vesicular stomatitis virus (VSV) matrix (M) protein mutants. One mutant M protein includes a glycine changed to a glutamic acid at position (21) a leucine changed to a phenylalanine at position (111) and a methionine changed to an arginine at position (51). Another M protein mutant includes a glycine changed to a glutamic acid at position (22) and a methionine changed to an arginine at positions (48) and (51). Yet another VSV M protein mutant includes a glycine changed to a glutamic acid at position (22) a leucine changed to a phenylalanine at position (110) and a methionine changed to an arginine at positions (48) and (51). The present invention is directed also to recombinant VSVs (rVSV) having these M mutants and to vaccines based on the rVSV having the M mutants of the present invention. These new rVSVs having the mutant M were significantly attenuated and lost virulence including neurovirulence and are capable of inducing an immune responses against an antigen of interest. In addition a rVSV serotype Indiana having the first described M mutant is capable of efficient replication at 31°C and of poor replication or incapable of replication at about 37°C or higher.
IN5656DEN2014 2011-12-23 2012-12-21 IN2014DN05656A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161579902P 2011-12-23 2011-12-23
PCT/CA2012/001185 WO2013091080A1 (en) 2011-12-23 2012-12-21 Vesicular stomatitis virus for prime boost vaccines

Publications (1)

Publication Number Publication Date
IN2014DN05656A true IN2014DN05656A (en) 2015-04-03

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IN5656DEN2014 IN2014DN05656A (en) 2011-12-23 2012-12-21

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US (4) US9630996B2 (en)
EP (1) EP2794637B1 (en)
KR (1) KR101989978B1 (en)
CN (1) CN104271594B (en)
AP (1) AP2014007776A0 (en)
BR (1) BR112014015390B1 (en)
CA (1) CA2860297C (en)
ES (1) ES2640961T3 (en)
IN (1) IN2014DN05656A (en)
WO (1) WO2013091080A1 (en)
ZA (1) ZA201405177B (en)

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EP3632463A1 (en) 2011-04-08 2020-04-08 Immune Design Corp. Immunogenic compositions and methods of using the compositions for inducing humoral and cellular immune responses
FR2987836A1 (en) * 2012-03-09 2013-09-13 Biomerieux Sa INTERFERENCE PEPTIDES AND METHOD FOR DETECTING MICROORGANISMS
EP3013960B1 (en) * 2013-06-26 2025-09-03 The University of Western Ontario Modified matrix proteins of vesicular stomatitis virus
WO2019183802A1 (en) * 2018-03-27 2019-10-03 苏州奥特铭医药科技有限公司 Attenuated strains of oncolytic rhabdovirus and uses thereof in tumor treatment
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CN110577585A (en) * 2018-06-07 2019-12-17 中国医学科学院基础医学研究所 Vesicular stomatitis virus envelope glycoprotein variant and its construction method and application
WO2020029274A1 (en) * 2018-08-10 2020-02-13 苏州奥特铭医药科技有限公司 Preparation method for attenuated baculovirus and application thereof
CA3179213A1 (en) * 2020-04-16 2021-10-21 Sumagen Canada Inc. Mers-cov vaccine
WO2021212101A1 (en) 2020-04-17 2021-10-21 Vyriad, Inc. Compositions for treating and/or preventing coronavirus infections
CA3180508A1 (en) 2020-04-17 2021-10-21 Regeneron Pharmaceuticals, Inc. Detection assays for coronavirus neutralizing antibodies
CN111286493B (en) 2020-05-12 2020-10-27 上海荣瑞医药科技有限公司 An oncolytic virus vaccine and its drug combined with immune cells to treat tumors
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