IN2014DE00911A - - Google Patents

Info

Publication number
IN2014DE00911A
IN2014DE00911A IN911DE2014A IN2014DE00911A IN 2014DE00911 A IN2014DE00911 A IN 2014DE00911A IN 911DE2014 A IN911DE2014 A IN 911DE2014A IN 2014DE00911 A IN2014DE00911 A IN 2014DE00911A
Authority
IN
India
Prior art keywords
digestion
amplicon
digestion product
scparately
bamhi
Prior art date
Application number
Inventor
Varma Basil Dr Mandira
Garima Kushal
Kumar Dhar Dwivedi Shailendra
Pathak Rakesh
Mridula Bose Professor
Rajendra Prasad Professor
Original Assignee
Vallabhbhai Patel Chest Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vallabhbhai Patel Chest Inst filed Critical Vallabhbhai Patel Chest Inst
Priority to IN911DE2014 priority Critical patent/IN2014DE00911A/en
Priority to US14/671,686 priority patent/US20150299779A1/en
Publication of IN2014DE00911A publication Critical patent/IN2014DE00911A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

The present invention relates to a kit and a method to identify M.tuberculosis.The method includes the following steps: a. isolating chromosomal DNA from a biological sample (Culture) b. conducting a real time PCR to obtain an amplicon c. digestion of said amplicon scparately with restriction enzymes Nrul and BamHI to obtain a digestion product d. Separating said digestion product into its constituents in an agarose gel; and e. Analyzing the restriction enzyme digestion pattern
IN911DE2014 2014-03-29 2014-03-29 IN2014DE00911A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
IN911DE2014 IN2014DE00911A (en) 2014-03-29 2014-03-29
US14/671,686 US20150299779A1 (en) 2014-03-29 2015-03-27 Method to identify m. tuberculosis

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IN911DE2014 IN2014DE00911A (en) 2014-03-29 2014-03-29

Publications (1)

Publication Number Publication Date
IN2014DE00911A true IN2014DE00911A (en) 2015-10-02

Family

ID=54321498

Family Applications (1)

Application Number Title Priority Date Filing Date
IN911DE2014 IN2014DE00911A (en) 2014-03-29 2014-03-29

Country Status (2)

Country Link
US (1) US20150299779A1 (en)
IN (1) IN2014DE00911A (en)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2378953A1 (en) * 1999-07-27 2001-02-01 Abgenix, Inc. Methods and compositions for inhibiting polypeptide accumulation associated with neurological disorders

Also Published As

Publication number Publication date
US20150299779A1 (en) 2015-10-22

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