IN2012DN03431A - - Google Patents
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- Publication number
- IN2012DN03431A IN2012DN03431A IN3431DEN2012A IN2012DN03431A IN 2012DN03431 A IN2012DN03431 A IN 2012DN03431A IN 3431DEN2012 A IN3431DEN2012 A IN 3431DEN2012A IN 2012DN03431 A IN2012DN03431 A IN 2012DN03431A
- Authority
- IN
- India
- Prior art keywords
- cells
- container
- magnetic stir
- stir element
- disclosed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27239609P | 2009-09-21 | 2009-09-21 | |
PCT/US2010/002569 WO2011034621A2 (en) | 2009-09-21 | 2010-09-20 | Magnetic lysis method and device |
Publications (1)
Publication Number | Publication Date |
---|---|
IN2012DN03431A true IN2012DN03431A (en) | 2015-10-23 |
Family
ID=43756937
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IN3431DEN2012 IN2012DN03431A (en) | 2009-09-21 | 2010-09-20 |
Country Status (8)
Country | Link |
---|---|
US (4) | US8399190B2 (en) |
EP (2) | EP3067112B1 (en) |
JP (1) | JP2013505018A (en) |
CN (2) | CN102686306B (en) |
CA (2) | CA2811872C (en) |
ES (1) | ES2709123T3 (en) |
IN (1) | IN2012DN03431A (en) |
WO (1) | WO2011034621A2 (en) |
Families Citing this family (63)
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US9132394B2 (en) | 2008-09-23 | 2015-09-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US8951939B2 (en) | 2011-07-12 | 2015-02-10 | Bio-Rad Laboratories, Inc. | Digital assays with multiplexed detection of two or more targets in the same optical channel |
US9417190B2 (en) | 2008-09-23 | 2016-08-16 | Bio-Rad Laboratories, Inc. | Calibrations and controls for droplet-based assays |
US9764322B2 (en) | 2008-09-23 | 2017-09-19 | Bio-Rad Laboratories, Inc. | System for generating droplets with pressure monitoring |
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US8633015B2 (en) * | 2008-09-23 | 2014-01-21 | Bio-Rad Laboratories, Inc. | Flow-based thermocycling system with thermoelectric cooler |
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US11130128B2 (en) | 2008-09-23 | 2021-09-28 | Bio-Rad Laboratories, Inc. | Detection method for a target nucleic acid |
US9492797B2 (en) | 2008-09-23 | 2016-11-15 | Bio-Rad Laboratories, Inc. | System for detection of spaced droplets |
US8709762B2 (en) | 2010-03-02 | 2014-04-29 | Bio-Rad Laboratories, Inc. | System for hot-start amplification via a multiple emulsion |
WO2011120020A1 (en) | 2010-03-25 | 2011-09-29 | Quantalife, Inc. | Droplet transport system for detection |
US9598725B2 (en) | 2010-03-02 | 2017-03-21 | Bio-Rad Laboratories, Inc. | Emulsion chemistry for encapsulated droplets |
US10512910B2 (en) | 2008-09-23 | 2019-12-24 | Bio-Rad Laboratories, Inc. | Droplet-based analysis method |
WO2010077322A1 (en) | 2008-12-31 | 2010-07-08 | Microchip Biotechnologies, Inc. | Instrument with microfluidic chip |
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SG176669A1 (en) | 2009-06-05 | 2012-01-30 | Integenx Inc | Universal sample preparation system and use in an integrated analysis system |
EP2940153B1 (en) | 2009-09-02 | 2020-05-13 | Bio-Rad Laboratories, Inc. | System for mixing fluids by coalescence of multiple emulsions |
WO2011034621A2 (en) * | 2009-09-21 | 2011-03-24 | Akonni Biosystems | Magnetic lysis method and device |
US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
EP2550528B1 (en) | 2010-03-25 | 2019-09-11 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
CA2767113A1 (en) | 2010-03-25 | 2011-09-29 | Bio-Rad Laboratories, Inc. | Detection system for droplet-based assays |
US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
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US8763642B2 (en) | 2010-08-20 | 2014-07-01 | Integenx Inc. | Microfluidic devices with mechanically-sealed diaphragm valves |
JP5922139B2 (en) | 2010-11-01 | 2016-05-24 | バイオ−ラッド・ラボラトリーズ・インコーポレーテッド | System for forming an emulsion |
US9150826B2 (en) * | 2011-03-14 | 2015-10-06 | Zymo Research Corporation | Portable sample disruptor apparatus, kits, and methods |
CN103534360A (en) | 2011-03-18 | 2014-01-22 | 伯乐生命医学产品有限公司 | Multiplexed digital assays with combinatorial use of signals |
CA3119914C (en) | 2011-04-13 | 2024-02-27 | Akonni Biosystems, Inc. | Microarray based sample detection system |
EP2702175B1 (en) | 2011-04-25 | 2018-08-08 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
EP2737089B1 (en) | 2011-07-29 | 2017-09-06 | Bio-rad Laboratories, Inc. | Library characterization by digital assay |
CN106423314B (en) | 2011-09-28 | 2021-03-02 | 哈佛学院院长等 | Systems and methods for droplet generation and/or fluid manipulation |
KR101548124B1 (en) * | 2011-10-04 | 2015-08-31 | (주) 메디컬그룹베스티안 | Cell processing and delivery system for alleviating or treating of skin defects |
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US10865440B2 (en) | 2011-10-21 | 2020-12-15 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
US9707528B2 (en) | 2012-02-22 | 2017-07-18 | T2 Biosystems, Inc. | Containers for agitation of liquid samples and methods of use thereof |
US9333471B2 (en) * | 2012-04-11 | 2016-05-10 | STAT—Diagnostica & Innovation, S.L. | Fluidically integrated magnetic bead beater |
US9304066B2 (en) * | 2012-04-11 | 2016-04-05 | Stat-Diagnostica & Innovation S.L. | Fluidically integrated rotary bead beader |
US9580679B2 (en) | 2012-09-21 | 2017-02-28 | California Institute Of Technology | Methods and devices for sample lysis |
EP2948249A1 (en) | 2013-01-22 | 2015-12-02 | University of Washington through its Center for Commercialization | Sequential delivery of fluid volumes and associated devices, systems and methods |
CN110560187B (en) | 2013-11-18 | 2022-01-11 | 尹特根埃克斯有限公司 | Cartridge and instrument for sample analysis |
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JP7216652B2 (en) * | 2017-01-30 | 2023-02-01 | セイフガード バイオシステムズ ホールディングズ リミテッド | Bead crushing tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
CA3052096A1 (en) * | 2017-01-30 | 2018-08-02 | Safeguard Biosystems Holdings Ltd. | Bead beating tube and method for extracting deoxyribonucleic acid and/or ribonucleic acid from microorganisms |
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US10610843B2 (en) | 2017-11-28 | 2020-04-07 | Talis Biomedical Corporation | Magnetic mixing apparatus |
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WO2011034621A2 (en) * | 2009-09-21 | 2011-03-24 | Akonni Biosystems | Magnetic lysis method and device |
-
2010
- 2010-09-20 WO PCT/US2010/002569 patent/WO2011034621A2/en active Application Filing
- 2010-09-20 CA CA2811872A patent/CA2811872C/en active Active
- 2010-09-20 ES ES16166269T patent/ES2709123T3/en active Active
- 2010-09-20 EP EP16166269.7A patent/EP3067112B1/en active Active
- 2010-09-20 EP EP10817590.2A patent/EP2480324A4/en not_active Ceased
- 2010-09-20 US US12/886,144 patent/US8399190B2/en active Active
- 2010-09-20 CA CA3041540A patent/CA3041540C/en active Active
- 2010-09-20 IN IN3431DEN2012 patent/IN2012DN03431A/en unknown
- 2010-09-20 JP JP2012529754A patent/JP2013505018A/en active Pending
- 2010-09-20 CN CN201080042060.XA patent/CN102686306B/en active Active
- 2010-09-20 CN CN201510589994.XA patent/CN105969761B/en active Active
-
2013
- 2013-02-12 US US13/765,399 patent/US9217174B2/en active Active
-
2015
- 2015-12-22 US US14/978,562 patent/US10138458B2/en active Active
-
2018
- 2018-09-24 US US16/140,171 patent/US11118156B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
ES2709123T3 (en) | 2019-04-15 |
EP3067112A1 (en) | 2016-09-14 |
US9217174B2 (en) | 2015-12-22 |
CN102686306A (en) | 2012-09-19 |
CN102686306B (en) | 2015-11-25 |
CA3041540C (en) | 2021-08-24 |
CA3041540A1 (en) | 2011-03-24 |
CN105969761A (en) | 2016-09-28 |
US20130157274A1 (en) | 2013-06-20 |
EP3067112B1 (en) | 2018-12-26 |
CA2811872C (en) | 2019-06-18 |
EP2480324A4 (en) | 2013-10-02 |
WO2011034621A3 (en) | 2011-11-24 |
JP2013505018A (en) | 2013-02-14 |
EP2480324A2 (en) | 2012-08-01 |
US20110070589A1 (en) | 2011-03-24 |
CA2811872A1 (en) | 2011-03-24 |
US11118156B2 (en) | 2021-09-14 |
US8399190B2 (en) | 2013-03-19 |
WO2011034621A2 (en) | 2011-03-24 |
US20120003654A2 (en) | 2012-01-05 |
US20160215255A1 (en) | 2016-07-28 |
CN105969761B (en) | 2020-04-07 |
US20190024039A1 (en) | 2019-01-24 |
US10138458B2 (en) | 2018-11-27 |
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