IN2012DN00581A - - Google Patents

Download PDF

Info

Publication number
IN2012DN00581A
IN2012DN00581A IN581DEN2012A IN2012DN00581A IN 2012DN00581 A IN2012DN00581 A IN 2012DN00581A IN 581DEN2012 A IN581DEN2012 A IN 581DEN2012A IN 2012DN00581 A IN2012DN00581 A IN 2012DN00581A
Authority
IN
India
Prior art keywords
compositions
generation
methods
stem cells
pluripotent stem
Prior art date
Application number
Inventor
Alessandra Giorgetti
Belmonte Juan Carlos Izpisua
Original Assignee
Salk Inst For Biological Studi
Ct Of Regenerative Medicine Of Barcelona Spain
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Inst For Biological Studi, Ct Of Regenerative Medicine Of Barcelona Spain filed Critical Salk Inst For Biological Studi
Publication of IN2012DN00581A publication Critical patent/IN2012DN00581A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/14—Blood; Artificial blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/602—Sox-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/603—Oct-3/4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/604—Klf-4
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
    • C12N2501/606—Transcription factors c-Myc
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1323—Adult fibroblasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin

Abstract

Methods and compositions for the generation and use of genetically corrected induced pluripotent stem cells are provided.
IN581DEN2012 2009-06-19 2010-06-18 IN2012DN00581A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21861109P 2009-06-19 2009-06-19
PCT/US2010/039222 WO2010148334A2 (en) 2009-06-19 2010-06-18 Generation of induced pluripotent stem cells from cord blood

Publications (1)

Publication Number Publication Date
IN2012DN00581A true IN2012DN00581A (en) 2015-06-12

Family

ID=43357075

Family Applications (1)

Application Number Title Priority Date Filing Date
IN581DEN2012 IN2012DN00581A (en) 2009-06-19 2010-06-18

Country Status (12)

Country Link
US (1) US20110044961A1 (en)
EP (1) EP2443231A4 (en)
JP (1) JP2012530497A (en)
KR (1) KR20120099363A (en)
CN (1) CN102712903A (en)
AU (1) AU2010263055A1 (en)
CA (1) CA2766049A1 (en)
IL (1) IL217063A0 (en)
IN (1) IN2012DN00581A (en)
RU (1) RU2012101874A (en)
SG (1) SG176925A1 (en)
WO (1) WO2010148334A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2548950T3 (en) 2009-06-05 2017-12-11 Cellular Dynamics Int Inc Reprogramming of T cells and hematopoietic cells
WO2011032166A2 (en) * 2009-09-14 2011-03-17 The Johns Hopkins University Reprogramming blood cells to pluripotent and multipotent stem cells
CN104781396A (en) * 2012-01-17 2015-07-15 小利兰·斯坦福大学托管委员会 Activation of innate immunity for nuclear reprogramming of somatic cells
WO2015069736A1 (en) 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
GB201704953D0 (en) 2017-03-28 2017-05-10 Imp Innovations Ltd Natural killer cells
WO2018232079A1 (en) 2017-06-14 2018-12-20 Daley George Q Hematopoietic stem and progenitor cells derived from hemogenic endothelial cells by episomal plasmid gene transfer
WO2019113169A2 (en) 2017-12-06 2019-06-13 New York Blood Center, Inc. HLA HOMOZYGOUS INDUCED PLURIPOTENT STEM CELL (iPSC) LIBRARIES
IL294715A (en) 2020-01-23 2022-09-01 Childrens Medical Ct Corp Stroma-free t cell differentiation from human pluripotent stem cells
WO2023285529A1 (en) 2021-07-13 2023-01-19 Arne Jensen Method of producing immortalized clonal mesenchymal stem cells (hmscs) from hla homozygous human induced pluripotent stem cells (hipsc's) derived from cord blood
WO2023285533A1 (en) 2021-07-13 2023-01-19 Arne Jensen Method of producing exosomes from immortalized clonal mesenchymal stem cells (hmscs) derived from hla homozygous human induced pluripotent stem cells (hipsc's) derived from cord blood
WO2023285536A1 (en) 2021-07-13 2023-01-19 Arne Jensen Exosomes derived from immortalized mesenchymal stromal cells (hmscs) for use as a medicament
CN113699183A (en) * 2021-08-06 2021-11-26 温州医科大学 Recombinant plasmid for marking and tracking CD133 positive ependymal cell, construction method and identification method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1176189A1 (en) * 2000-07-21 2002-01-30 Fornix Biosciences N.V. Stem cell-like cells
EP2164951A2 (en) * 2007-05-30 2010-03-24 The General Hospital Corporation Methods of generating pluripotent cells from somatic cells
JP2008307007A (en) * 2007-06-15 2008-12-25 Bayer Schering Pharma Ag Human pluripotent stem cell induced from human tissue-originated undifferentiated stem cell after birth
WO2010111422A2 (en) * 2009-03-25 2010-09-30 The Salk Institute For Biological Studies Induced pluripotent stem cell generation using two factors and p53 inactivation

Also Published As

Publication number Publication date
CN102712903A (en) 2012-10-03
WO2010148334A2 (en) 2010-12-23
AU2010263055A1 (en) 2012-02-23
RU2012101874A (en) 2013-07-27
KR20120099363A (en) 2012-09-10
CA2766049A1 (en) 2010-12-23
IL217063A0 (en) 2012-02-29
EP2443231A2 (en) 2012-04-25
JP2012530497A (en) 2012-12-06
EP2443231A4 (en) 2013-02-27
US20110044961A1 (en) 2011-02-24
WO2010148334A3 (en) 2011-05-19
SG176925A1 (en) 2012-01-30

Similar Documents

Publication Publication Date Title
IN2012DN00581A (en)
WO2010111409A3 (en) Pluripotent stem cells
ZA201606403B (en) Pluripotent stem cell culture on micro-carriers
WO2010111422A3 (en) Induced pluripotent stem cell generation using two factors and p53 inactivation
EP2421956A4 (en) Compositions and methods for deriving or culturing pluripotent cells
IL214798A0 (en) Methods and compositions for stem cell cultures
SG196784A1 (en) Stem cell cultures
EP2373784A4 (en) Generation and maintenance of stem cells
ZA201202685B (en) Methods of preparing mesenchymal stem cells,compositions and kit thereof
MX350966B (en) Pluripotent stem cells.
MY159971A (en) Multipotent/pluripotent cells and methods
FI20096288A0 (en) Formulations and Methods for Culturing Stem Cells
EP2435558A4 (en) Generation of genetically corrected disease-free induced pluripotent stem cells
EP2440652A4 (en) Methods for culturing stem and progenitor cells
MX2010000348A (en) Single pluripotent stem cell culture.
PL2162534T3 (en) Genetically modified CD34 negative mesenchymal stem cells for tumour treatment
MX336996B (en) Certain triazolopyridines and triazolopyrazines, compositions thereof and methods of use therefor.
UA118328C2 (en) Targeted gemomic alteration
EP2494032A4 (en) Generating induced pluripotent stem cells and progenitor cells from fibroblasts
EP2387609A4 (en) Non-embryonic stem cells and uses thereof
IN2012DN00852A (en)
EP2424978A4 (en) Compositions and methods for modulating stem cells and uses thereof
WO2011100286A3 (en) Compositions and methods of generating a differentiated mesodermal cell
IL221586B (en) Smooth muscle-like cells (smlcs) derived from human pluripotent stem cells
IN2012DN03404A (en)