IL83523A - Method of purification of eimeria gametocytes and coccidiosis vaccine containing them - Google Patents
Method of purification of eimeria gametocytes and coccidiosis vaccine containing themInfo
- Publication number
- IL83523A IL83523A IL8352387A IL8352387A IL83523A IL 83523 A IL83523 A IL 83523A IL 8352387 A IL8352387 A IL 8352387A IL 8352387 A IL8352387 A IL 8352387A IL 83523 A IL83523 A IL 83523A
- Authority
- IL
- Israel
- Prior art keywords
- gametocytes
- gametocyte
- eimeria
- chicken
- maxima
- Prior art date
Links
- 210000000973 gametocyte Anatomy 0.000 title claims abstract description 271
- 238000000034 method Methods 0.000 title claims abstract description 98
- 241000223924 Eimeria Species 0.000 title claims abstract description 44
- 229960005486 vaccine Drugs 0.000 title claims abstract description 34
- 208000003495 Coccidiosis Diseases 0.000 title description 7
- 206010023076 Isosporiasis Diseases 0.000 title description 7
- 238000000746 purification Methods 0.000 title description 4
- 241000287828 Gallus gallus Species 0.000 claims abstract description 128
- 241000223934 Eimeria maxima Species 0.000 claims abstract description 73
- 208000015181 infectious disease Diseases 0.000 claims abstract description 72
- 230000036039 immunity Effects 0.000 claims abstract description 30
- 230000003053 immunization Effects 0.000 claims abstract description 30
- 210000000936 intestine Anatomy 0.000 claims description 42
- 230000000968 intestinal effect Effects 0.000 claims description 24
- 239000000872 buffer Substances 0.000 claims description 21
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 14
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 13
- 239000011148 porous material Substances 0.000 claims description 13
- 241001465754 Metazoa Species 0.000 claims description 11
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical group P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 9
- 239000002953 phosphate buffered saline Substances 0.000 claims description 9
- 108010003272 Hyaluronate lyase Proteins 0.000 claims description 8
- 102000001974 Hyaluronidases Human genes 0.000 claims description 8
- 229960002773 hyaluronidase Drugs 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 7
- 210000000055 macrogametocyte Anatomy 0.000 claims description 7
- 239000007983 Tris buffer Substances 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 235000011148 calcium chloride Nutrition 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 6
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 229940098773 bovine serum albumin Drugs 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 210000000052 microgametocyte Anatomy 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 3
- 229920002683 Glycosaminoglycan Polymers 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 229920002674 hyaluronan Polymers 0.000 claims description 3
- 229960003160 hyaluronic acid Drugs 0.000 claims description 3
- 238000007918 intramuscular administration Methods 0.000 claims description 3
- 239000007928 intraperitoneal injection Substances 0.000 claims description 3
- 238000001990 intravenous administration Methods 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 2
- 239000007927 intramuscular injection Substances 0.000 claims description 2
- 238000000527 sonication Methods 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 abstract description 130
- 102000004169 proteins and genes Human genes 0.000 abstract description 129
- 210000003250 oocyst Anatomy 0.000 abstract description 97
- 239000000284 extract Substances 0.000 abstract description 68
- 238000011161 development Methods 0.000 abstract description 27
- 238000000338 in vitro Methods 0.000 abstract description 27
- 238000002360 preparation method Methods 0.000 abstract description 18
- 229940079593 drug Drugs 0.000 abstract description 14
- 239000003814 drug Substances 0.000 abstract description 14
- 235000013330 chicken meat Nutrition 0.000 description 120
- 239000000427 antigen Substances 0.000 description 109
- 102000036639 antigens Human genes 0.000 description 109
- 108091007433 antigens Proteins 0.000 description 109
- 210000002966 serum Anatomy 0.000 description 53
- 241000271566 Aves Species 0.000 description 49
- 238000002474 experimental method Methods 0.000 description 42
- 238000001262 western blot Methods 0.000 description 37
- 241000283973 Oryctolagus cuniculus Species 0.000 description 34
- 244000045947 parasite Species 0.000 description 29
- 210000003046 sporozoite Anatomy 0.000 description 28
- 239000000047 product Substances 0.000 description 25
- 210000001519 tissue Anatomy 0.000 description 25
- 241000699666 Mus <mouse, genus> Species 0.000 description 22
- 108010048090 soybean lectin Proteins 0.000 description 22
- 238000002965 ELISA Methods 0.000 description 19
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 17
- 238000012360 testing method Methods 0.000 description 17
- 238000013519 translation Methods 0.000 description 17
- 239000007924 injection Substances 0.000 description 16
- 238000002347 injection Methods 0.000 description 16
- 210000003936 merozoite Anatomy 0.000 description 16
- 230000000694 effects Effects 0.000 description 15
- 108020004999 messenger RNA Proteins 0.000 description 15
- 230000001681 protective effect Effects 0.000 description 15
- 239000003599 detergent Substances 0.000 description 14
- 230000012010 growth Effects 0.000 description 14
- 238000002649 immunization Methods 0.000 description 14
- 238000004519 manufacturing process Methods 0.000 description 14
- 241000894007 species Species 0.000 description 14
- 108090001090 Lectins Proteins 0.000 description 13
- 102000004856 Lectins Human genes 0.000 description 13
- 238000001727 in vivo Methods 0.000 description 13
- 210000000056 organ Anatomy 0.000 description 13
- 239000002523 lectin Substances 0.000 description 12
- 239000000463 material Substances 0.000 description 12
- 238000001556 precipitation Methods 0.000 description 12
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 12
- 230000027455 binding Effects 0.000 description 11
- 241000699670 Mus sp. Species 0.000 description 9
- 230000000890 antigenic effect Effects 0.000 description 9
- 238000011534 incubation Methods 0.000 description 9
- 150000002632 lipids Chemical class 0.000 description 9
- 239000002671 adjuvant Substances 0.000 description 8
- 210000003711 chorioallantoic membrane Anatomy 0.000 description 8
- 238000010790 dilution Methods 0.000 description 8
- 239000012895 dilution Substances 0.000 description 8
- 230000008774 maternal effect Effects 0.000 description 8
- 230000036961 partial effect Effects 0.000 description 8
- 239000013641 positive control Substances 0.000 description 8
- 239000002299 complementary DNA Substances 0.000 description 7
- 235000013601 eggs Nutrition 0.000 description 7
- 238000010166 immunofluorescence Methods 0.000 description 7
- 210000004877 mucosa Anatomy 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 102000003886 Glycoproteins Human genes 0.000 description 6
- 108090000288 Glycoproteins Proteins 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 6
- 239000001166 ammonium sulphate Substances 0.000 description 6
- 235000011130 ammonium sulphate Nutrition 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 229920001220 nitrocellulos Polymers 0.000 description 6
- 230000009257 reactivity Effects 0.000 description 6
- 229920000936 Agarose Polymers 0.000 description 5
- 241000224483 Coccidia Species 0.000 description 5
- 241000223932 Eimeria tenella Species 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 230000004927 fusion Effects 0.000 description 5
- 230000028993 immune response Effects 0.000 description 5
- 238000011533 pre-incubation Methods 0.000 description 5
- 230000001568 sexual effect Effects 0.000 description 5
- 230000003442 weekly effect Effects 0.000 description 5
- 241000220317 Rosa Species 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000004587 chromatography analysis Methods 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 210000004201 immune sera Anatomy 0.000 description 4
- 229940042743 immune sera Drugs 0.000 description 4
- 230000002163 immunogen Effects 0.000 description 4
- 230000009545 invasion Effects 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 239000013642 negative control Substances 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 3
- 102000018832 Cytochromes Human genes 0.000 description 3
- 108010052832 Cytochromes Proteins 0.000 description 3
- 102000009109 Fc receptors Human genes 0.000 description 3
- 108010087819 Fc receptors Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- OVRNDRQMDRJTHS-CBQIKETKSA-N N-Acetyl-D-Galactosamine Chemical compound CC(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@H](O)[C@@H]1O OVRNDRQMDRJTHS-CBQIKETKSA-N 0.000 description 3
- MBLBDJOUHNCFQT-UHFFFAOYSA-N N-acetyl-D-galactosamine Natural products CC(=O)NC(C=O)C(O)C(O)C(O)CO MBLBDJOUHNCFQT-UHFFFAOYSA-N 0.000 description 3
- 239000012980 RPMI-1640 medium Substances 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 210000004671 cell-free system Anatomy 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 239000003224 coccidiostatic agent Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 102000013415 peroxidase activity proteins Human genes 0.000 description 3
- 108040007629 peroxidase activity proteins Proteins 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 102000005962 receptors Human genes 0.000 description 3
- 108020003175 receptors Proteins 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 230000002829 reductive effect Effects 0.000 description 3
- 210000001995 reticulocyte Anatomy 0.000 description 3
- 210000001563 schizont Anatomy 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 229940031626 subunit vaccine Drugs 0.000 description 3
- 102000014914 Carrier Proteins Human genes 0.000 description 2
- 108010062580 Concanavalin A Proteins 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 2
- 108010057081 Merozoite Surface Protein 1 Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 229920005654 Sephadex Polymers 0.000 description 2
- 239000012507 Sephadex™ Substances 0.000 description 2
- 108010046516 Wheat Germ Agglutinins Proteins 0.000 description 2
- 229940037003 alum Drugs 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000002238 attenuated effect Effects 0.000 description 2
- 108091008324 binding proteins Proteins 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000004440 column chromatography Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
- 230000001506 immunosuppresive effect Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 201000004792 malaria Diseases 0.000 description 2
- 229960004452 methionine Drugs 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000003127 radioimmunoassay Methods 0.000 description 2
- 239000012146 running buffer Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000000954 titration curve Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- UMCMPZBLKLEWAF-BCTGSCMUSA-N 3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 UMCMPZBLKLEWAF-BCTGSCMUSA-N 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- HKJKONMZMPUGHJ-UHFFFAOYSA-N 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC2=CC(S(O)(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 HKJKONMZMPUGHJ-UHFFFAOYSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000224482 Apicomplexa Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 208000031504 Asymptomatic Infections Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 238000009010 Bradford assay Methods 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 229920001412 Chicle Polymers 0.000 description 1
- XZMCDFZZKTWFGF-UHFFFAOYSA-N Cyanamide Chemical compound NC#N XZMCDFZZKTWFGF-UHFFFAOYSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000499566 Eimeria brunetti Species 0.000 description 1
- 241000224484 Eimeriidae Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 102000028555 IgG binding proteins Human genes 0.000 description 1
- 108091009325 IgG binding proteins Proteins 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 240000001794 Manilkara zapota Species 0.000 description 1
- 235000011339 Manilkara zapota Nutrition 0.000 description 1
- 206010048685 Oral infection Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 241000224016 Plasmodium Species 0.000 description 1
- 241000276498 Pollachius virens Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 208000035415 Reinfection Diseases 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 229940117913 acrylamide Drugs 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 230000001165 anti-coccidial effect Effects 0.000 description 1
- 230000027645 antigenic variation Effects 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- 210000003555 cloaca Anatomy 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical group BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 210000000990 duodenal loop Anatomy 0.000 description 1
- 210000002969 egg yolk Anatomy 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 210000004317 gizzard Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000008004 immune attack Effects 0.000 description 1
- 230000016784 immunoglobulin production Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 244000000056 intracellular parasite Species 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 229940124590 live attenuated vaccine Drugs 0.000 description 1
- 229940023012 live-attenuated vaccine Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000000054 macrogamete Anatomy 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000001466 metabolic labeling Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000007479 molecular analysis Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000037452 priming Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 230000004580 weight loss Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/20—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans from protozoa
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/002—Protozoa antigens
- A61K39/012—Coccidia antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P33/00—Antiparasitic agents
- A61P33/02—Antiprotozoals, e.g. for leishmaniasis, trichomoniasis, toxoplasmosis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
- C07K14/455—Eimeria
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F02—COMBUSTION ENGINES; HOT-GAS OR COMBUSTION-PRODUCT ENGINE PLANTS
- F02B—INTERNAL-COMBUSTION PISTON ENGINES; COMBUSTION ENGINES IN GENERAL
- F02B75/00—Other engines
- F02B75/02—Engines characterised by their cycles, e.g. six-stroke
- F02B2075/022—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle
- F02B2075/027—Engines characterised by their cycles, e.g. six-stroke having less than six strokes per cycle four
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Genetics & Genomics (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Epidemiology (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IL108419A IL108419A (en) | 1986-08-14 | 1987-08-13 | Proteinaceous extract derived from eimeria maxima gametocytes and vaccines against eimeria maxima containing the same |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US89661186A | 1986-08-14 | 1986-08-14 |
Publications (1)
Publication Number | Publication Date |
---|---|
IL83523A true IL83523A (en) | 1994-10-21 |
Family
ID=25406502
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL8352387A IL83523A (en) | 1986-08-14 | 1987-08-13 | Method of purification of eimeria gametocytes and coccidiosis vaccine containing them |
IL10841994A IL108419A0 (en) | 1986-08-14 | 1994-01-24 | Proteinaceous extract derived from eimeria maxima gametocytes and vaccines against eimeria maxima containing the same |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL10841994A IL108419A0 (en) | 1986-08-14 | 1994-01-24 | Proteinaceous extract derived from eimeria maxima gametocytes and vaccines against eimeria maxima containing the same |
Country Status (10)
Country | Link |
---|---|
EP (1) | EP0256536B1 (de) |
JP (3) | JP2617946B2 (de) |
AT (1) | ATE132754T1 (de) |
AU (1) | AU633244B2 (de) |
CA (1) | CA1340838C (de) |
DE (1) | DE3751670T2 (de) |
ES (1) | ES2084576T3 (de) |
GR (1) | GR3019619T3 (de) |
IL (2) | IL83523A (de) |
ZA (1) | ZA876027B (de) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5496550A (en) * | 1986-08-14 | 1996-03-05 | Chilwalner | Method of reducing the output of Eimeria oocysts from a newborn chick |
ZA894726B (en) * | 1988-06-27 | 1990-03-28 | Akzo Nv | Coccidiosis vaccine |
NZ232372A (en) * | 1989-02-10 | 1993-04-28 | Merck & Co Inc | Glycolipid-linked membrane-associated immunogens of eimeria useful in immunising poultry against coccidiosis |
US6008342A (en) * | 1991-07-12 | 1999-12-28 | Roche Vitamins Inc. | DNA encoding eimeria antigen |
US5403581A (en) * | 1991-07-12 | 1995-04-04 | Hoffmann-La Roche Inc. | Coccidiosis vaccines |
NZ272945A (en) * | 1994-09-16 | 1997-12-19 | Akzo Nobel Nv | Eimeria t-lymphocyte stimulating protein and coding sequence, its production and use |
DE19828322A1 (de) * | 1998-06-25 | 1999-12-30 | Hoechst Roussel Vet Gmbh | Coccidienvakzine, Verfahren zu ihrer Herstellung und ihre Verwendung |
ZA200400479B (en) | 2001-07-06 | 2006-05-31 | Abic Biolog Lab Teva | Nucleic acids encoding recombinant 56 a 82 KDA antigens from gametocytes of Eimeria maxima and their use |
RU2004103468A (ru) | 2001-07-06 | 2005-04-20 | Абик Байолоджикал Лэборетериз Тева Лтд. (Il) | Нуклеиновые кислоты, кодирующие рекомбинантный 250 к-да-антиген спорозоитов/мерозоитов eimeria maxima, и их применения |
NZ759799A (en) * | 2014-04-10 | 2022-12-23 | MEP Equine Solutions LLC | Method for the quantification of parasite eggs in feces |
CN113528348B (zh) * | 2021-08-17 | 2022-04-22 | 中国农业科学院兰州兽医研究所 | 一种分离艾美尔球虫小配子的方法和应用 |
CN114058511A (zh) * | 2021-11-30 | 2022-02-18 | 中国农业科学院兰州兽医研究所 | 一种利用dna探针分离球虫小配子的方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2849226A1 (de) | 1977-11-14 | 1979-05-17 | Unilever Nv | Verfahren zur wachstumsfoerderung von gefluegel, mittel zu seiner durchfuehrung und deren herstellung |
EP0135073A3 (de) | 1983-08-19 | 1987-05-27 | American Cyanamid Company | Gegen Merozoiten von Eimeria Spp. reaktive monoklonale Antikörper und Antigene |
EP0135712A3 (de) | 1983-08-19 | 1987-05-27 | American Cyanamid Company | Gegen Sporozoiten von Eimeria Spp. reaktive monoklonale Antikörper und Antigene |
JPS6048924A (ja) | 1983-08-24 | 1985-03-16 | Takeda Chem Ind Ltd | 骨粗鬆症治療剤 |
CA1340520C (en) | 1984-06-05 | 1999-05-04 | Karel Z. Newman Jr. | Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by eimeria tenella and eimeria necatrix |
-
1987
- 1987-08-13 CA CA000544427A patent/CA1340838C/en not_active Expired - Fee Related
- 1987-08-13 IL IL8352387A patent/IL83523A/en not_active IP Right Cessation
- 1987-08-14 ES ES87111820T patent/ES2084576T3/es not_active Expired - Lifetime
- 1987-08-14 ZA ZA876027A patent/ZA876027B/xx unknown
- 1987-08-14 EP EP87111820A patent/EP0256536B1/de not_active Expired - Lifetime
- 1987-08-14 AT AT87111820T patent/ATE132754T1/de not_active IP Right Cessation
- 1987-08-14 DE DE3751670T patent/DE3751670T2/de not_active Expired - Lifetime
- 1987-08-14 JP JP62203006A patent/JP2617946B2/ja not_active Expired - Lifetime
- 1987-08-17 AU AU77121/87A patent/AU633244B2/en not_active Expired
-
1994
- 1994-01-24 IL IL10841994A patent/IL108419A0/xx unknown
-
1996
- 1996-04-10 GR GR960401016T patent/GR3019619T3/el unknown
- 1996-08-20 JP JP8218953A patent/JP2769313B2/ja not_active Expired - Lifetime
- 1996-08-20 JP JP8218952A patent/JPH09117282A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
AU633244B2 (en) | 1993-01-28 |
ATE132754T1 (de) | 1996-01-15 |
JPH09117282A (ja) | 1997-05-06 |
CA1340838C (en) | 1999-12-07 |
ZA876027B (en) | 1988-04-27 |
JP2769313B2 (ja) | 1998-06-25 |
EP0256536A3 (en) | 1989-11-02 |
AU7712187A (en) | 1988-05-19 |
EP0256536A2 (de) | 1988-02-24 |
JPS63233928A (ja) | 1988-09-29 |
ES2084576T3 (es) | 1996-05-16 |
DE3751670T2 (de) | 1996-10-24 |
GR3019619T3 (en) | 1996-07-31 |
JP2617946B2 (ja) | 1997-06-11 |
IL108419A0 (en) | 1994-04-12 |
DE3751670D1 (de) | 1996-02-22 |
JPH09118700A (ja) | 1997-05-06 |
EP0256536B1 (de) | 1996-01-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5001225A (en) | Monoclonal antibodies to a pan-malarial antigen | |
US5496550A (en) | Method of reducing the output of Eimeria oocysts from a newborn chick | |
CA1270758A (en) | Coccidiosis vaccine | |
EP0164176B1 (de) | Antigenische Proteine, dieselben enthaltende Vakzine, schützende Antikörper dagegen für die Verhütung von durch Eimeria tenella verursachte Coccidiosis | |
US8470343B2 (en) | Immunogenic compositions and uses thereof | |
EP0231537A2 (de) | Antigene Proteine und diese enthaltende Impfstoffe zur Verhütung von Kokzidiose | |
US5028694A (en) | Antigenic proteins and vaccines containing them for prevention of coccidiosis caused by eimeria Eimeria necatrix and Eimeria tenella | |
EP0135712A2 (de) | Gegen Sporozoiten von Eimeria Spp. reaktive monoklonale Antikörper und Antigene | |
EP0256536B1 (de) | Coccidiosis-Impfstoff | |
EP0838522A2 (de) | Impfstoff gegen Geflügelkokzidose | |
US5677438A (en) | Coccidiosis vaccine | |
CA1340858C (en) | Recombinant and native group b eimeria tenella immunogens useful as coccidiosis vaccines | |
DK171259B1 (da) | Specifikke ekskretion-sekretions antigener af Toxoplasma gondii, translatiosprodukter deraf, fremgangsmåde til fremstilling deraf og diagnostiske anvendelser deraf | |
CA2135547A1 (en) | Coccidiosis poultry vaccine | |
US5885568A (en) | Coccidiosis poultry vaccine | |
WO2001013923A1 (en) | Malaria gpi anchors as vaccines anti-parasitic drugs and for use in diagnostics | |
CA1261285A (en) | Antigens and monoclonal antibodies reactive against sporozoites of eimeria spp | |
AU7479894A (en) | Novel eimeria antibodies and antigens and methods of using the same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
KB | Patent renewed | ||
KB | Patent renewed | ||
EXP | Patent expired |