IL41450A - Water insoluble enzyme systems and an electrode coated with such systems - Google Patents
Water insoluble enzyme systems and an electrode coated with such systemsInfo
- Publication number
- IL41450A IL41450A IL41450A IL4145073A IL41450A IL 41450 A IL41450 A IL 41450A IL 41450 A IL41450 A IL 41450A IL 4145073 A IL4145073 A IL 4145073A IL 41450 A IL41450 A IL 41450A
- Authority
- IL
- Israel
- Prior art keywords
- lower alkyl
- polymer
- hydroxy lower
- enzyme
- hydroxy
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title claims abstract 9
- 102000004190 Enzymes Human genes 0.000 title claims abstract 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract 4
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims abstract 16
- -1 alkyl methacrylates Chemical class 0.000 claims abstract 13
- 229920000642 polymer Polymers 0.000 claims abstract 13
- 125000003545 alkoxy group Chemical group 0.000 claims abstract 8
- 125000000217 alkyl group Chemical group 0.000 claims abstract 7
- 238000000576 coating method Methods 0.000 claims abstract 3
- 229920001477 hydrophilic polymer Polymers 0.000 claims abstract 3
- 239000012528 membrane Substances 0.000 claims abstract 3
- WOBHKFSMXKNTIM-UHFFFAOYSA-N Hydroxyethyl methacrylate Chemical compound CC(=C)C(=O)OCCO WOBHKFSMXKNTIM-UHFFFAOYSA-N 0.000 claims abstract 2
- 239000011248 coating agent Substances 0.000 claims abstract 2
- 239000011159 matrix material Substances 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 125000000954 2-hydroxyethyl group Chemical group [H]C([*])([H])C([H])([H])O[H] 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 claims 1
- 241000282320 Panthera leo Species 0.000 claims 1
- 239000011324 bead Substances 0.000 claims 1
- 229920006037 cross link polymer Polymers 0.000 claims 1
- 239000011148 porous material Substances 0.000 claims 1
- 239000000463 material Substances 0.000 abstract 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract 2
- 210000004369 blood Anatomy 0.000 abstract 2
- 239000008280 blood Substances 0.000 abstract 2
- 210000001124 body fluid Anatomy 0.000 abstract 2
- 239000010839 body fluid Substances 0.000 abstract 2
- 239000004202 carbamide Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 229920002818 (Hydroxyethyl)methacrylate Polymers 0.000 abstract 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 abstract 1
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 abstract 1
- 108010046334 Urease Proteins 0.000 abstract 1
- 150000001252 acrylic acid derivatives Chemical class 0.000 abstract 1
- 125000005250 alkyl acrylate group Chemical group 0.000 abstract 1
- 229910021529 ammonia Inorganic materials 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000178 monomer Substances 0.000 abstract 1
- 229920000139 polyethylene terephthalate Polymers 0.000 abstract 1
- 239000005020 polyethylene terephthalate Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 230000003014 reinforcing effect Effects 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/14—Dynamic membranes
- B01D69/141—Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
- B01D69/1411—Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes containing dispersed material in a continuous matrix
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/08—Hollow fibre membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D69/00—Semi-permeable membranes for separation processes or apparatus characterised by their form, structure or properties; Manufacturing processes specially adapted therefor
- B01D69/14—Dynamic membranes
- B01D69/141—Heterogeneous membranes, e.g. containing dispersed material; Mixed matrix membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
- C12Q1/002—Electrode membranes
Abstract
1415597 Reactions with absorbed enzymes NATIONAL PATENT DEVELOPMENT CORP 1 Feb 1973 [11 Feb 1972] 5197/73 Heading B1X [Also in Divisions C3 and G1] A process for subjecting a substrate e.g. blood or body fluid containing an enzymesusceptible material to the action of an enzyme comprises permitting the enzyme-susceptible material of the substrate to come into contact with the enzyme entrapped or encapsulated within a water-insoluble hydrophilic polymer of a monomer of the group consisting of hydroxy lower alkyl (i.e. C 1 -C 3 ) acrylates, hydroxy lower alkyl methacrylates, hydroxy lower alkoxy lower alkyl acrylates, hydroxy lower alkoxy lower alkyl methacrylates with or without a proportion of vinyl pyrrolidone, acrylamide, methacrylamide, N- lower alkyl acrylamide, N-lower alkyl methacrylamide, N-hydroxy lower alkyl acrylamide, and N-hydroxy lower alkyl methacrylamide. The water insoluble hydrophilic polymer may be in the form of a hollow tube having a relatively thin overall internal semipermeable membrane coating of a polymer as above, but enzyme free, the semi-permeable membrane being permeable to the enzyme-susceptible material but not to the enzyme. In Fig. 3 a tube 18, shown in crosssection, comprises a lumen 20, a urease containing polymer layer 22 containing a reinforcing sheathing of polyethylene terephthalate and, optionally, inner and outer coatings of water insoluble hydroxyethyl methacrylate polymer. When a solution of urea is forced through the lumen of the tube the solution diffuses to the enzyme entrapped tube and ammonia diffuses out of the outer surface of the tube thus enabling the purification of body fluids e.g. blood to eliminate urea.
[GB1415597A]
Claims (7)
1. An enzymatically-active, water insoluble, alcohol soluble, hydrophilic product comprised of a polymer and an enzyme permanently entrapped in the cross-linked polymeric matrix thereof, said polymer being a polymer of hydroxy lower alkyl aerylate, hydroxy lower alkyl methacrylate, hydroxy lower alkoxy lower alkyl aer late, and hydroxy lowe alkoxy lower alkyl methacrylate.
2. A product according to claim 1 wherein said polymer is a sparingly cross-linked polymer.
3. A product according : to claim 1 wherein the polymer is a polymer of 2-hydroxyethyl;methacrylate.
4. A product according to claim 3 in the form of hard porous beads.
5. composition comprising the product of claim 1 coated with a relatively thin membrane of a solid, enzyme-free, water- nsoluble, alcohol-soluble hydrophilic polymer of the group consisting of polymers of hydroxy lower alkyl aerylate, hydroxy lower alkyl methacrylate, hydroxy lower alkoxy lower alkyl aerylate, and hydroxy lower alkoxy lower alkyl methacrylate.
6. A composition according to claim 5 wherein said thin membrance is a polymer of 2-hydroxyethyl methacrylate.
7. An electrode having a first coating thereon of an enzymatically-active, solid, water-insoluble, alcohol-soluble, hydrophilic, porous material comprised of a polyme and an enzyme permanently entrapped in the cross-linked polymeric matrix thereof, said polymer being a polymer of hydroxy lower alkyl aerylate, hydroxy lower alkyl methacrylate 41450/2 hydroxy lower alkoxy lower alkyl aerylate, and hydroxy lower alkoxy lower alkyl methacrylate, and having thereover a
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US22548972A | 1972-02-11 | 1972-02-11 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL41450A0 IL41450A0 (en) | 1973-04-30 |
| IL41450A true IL41450A (en) | 1976-10-31 |
Family
ID=22845087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL41450A IL41450A (en) | 1972-02-11 | 1973-02-02 | Water insoluble enzyme systems and an electrode coated with such systems |
Country Status (12)
| Country | Link |
|---|---|
| JP (1) | JPS4892446A (en) |
| AU (1) | AU469805B2 (en) |
| BE (1) | BE795316A (en) |
| CA (1) | CA1007586A (en) |
| DE (1) | DE2305320B2 (en) |
| ES (1) | ES411763A1 (en) |
| FR (2) | FR2171109B1 (en) |
| GB (1) | GB1415597A (en) |
| IL (1) | IL41450A (en) |
| IT (1) | IT987011B (en) |
| NL (1) | NL7301897A (en) |
| ZA (1) | ZA73696B (en) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3925157A (en) * | 1972-07-12 | 1975-12-09 | Pfizer | Immobilized enzymes |
| CH593992A5 (en) * | 1972-12-23 | 1977-12-30 | Roehm Gmbh | |
| JPS5631951B2 (en) * | 1973-09-12 | 1981-07-24 | ||
| CA1032882A (en) * | 1973-09-12 | 1978-06-13 | Floyd E. Colvin | Porous cartridges with fixed enzymes |
| JPS5631949B2 (en) * | 1973-09-12 | 1981-07-24 | ||
| JPS5219327B2 (en) * | 1973-11-15 | 1977-05-27 | ||
| JPS50123833A (en) * | 1974-03-18 | 1975-09-29 | ||
| JPS5126285A (en) * | 1974-08-30 | 1976-03-04 | Japan Atomic Energy Res Inst | Koso mataha kintaiofukunda soseibutsu no seizohoho |
| JPS5215887A (en) * | 1975-07-23 | 1977-02-05 | Japan Atom Energy Res Inst | Process for immobilizing enzymes and microbial cells |
| DE2633259C3 (en) * | 1975-07-23 | 1984-11-15 | Japan Atomic Energy Research Institute, Tokio/Tokyo | Method for immobilizing enzymes or enzyme-containing cells |
| JPS5215885A (en) * | 1975-07-23 | 1977-02-05 | Japan Atom Energy Res Inst | Process for immobilizing enzymes or microbial cells |
| JPS5235773A (en) * | 1975-09-17 | 1977-03-18 | Omron Tateisi Electronics Co | Reactive semipermeable membrane |
| JPS5247983A (en) * | 1975-10-09 | 1977-04-16 | Kyowa Yuka Kk | Method of immobilizing yeast or microbial cells |
| JPS5294487A (en) * | 1976-01-31 | 1977-08-09 | Japan Atom Energy Res Inst | Production of compositions containing enzyme or microbial cells |
| JPS5296791A (en) * | 1976-02-09 | 1977-08-13 | Japan Atom Energy Res Inst | Preparation of composition including enzymes or microorganisms |
| GB2084899B (en) * | 1980-09-23 | 1985-05-30 | California Inst Of Techn | Apparatus and method for the sequential performance of chemical processes |
| EP0382767B1 (en) * | 1987-09-28 | 1993-12-15 | Novo Nordisk A/S | Method for immobilizing lipase |
| AT404992B (en) | 1997-04-17 | 1999-04-26 | Avl List Gmbh | SENSOR FOR DETERMINING AN ENZYME SUBSTRATE |
| CN103619371B (en) * | 2011-07-27 | 2016-06-29 | 旭化成医疗株式会社 | Hollow fiber membrane type blood purifier |
| WO2015199768A1 (en) * | 2014-06-24 | 2015-12-30 | Medtronic, Inc. | Stacked sorbent assembly |
| US20210063402A1 (en) * | 2019-08-29 | 2021-03-04 | AbulKalam Mohammed Shamsuddin | Screening method, device and kit for detecting mucosal carbohydrates and associated conditions |
| KR20220163450A (en) | 2020-04-03 | 2022-12-09 | 엘폭시 세라퓨틱스 인코퍼레이티드 | Intestinal aerobic therapy |
| WO2023056256A1 (en) * | 2021-09-29 | 2023-04-06 | Lpoxy Therapeutics, Inc. | Enteric aerobization therapy |
-
0
- BE BE795316D patent/BE795316A/en unknown
-
1973
- 1973-01-31 ZA ZA730696A patent/ZA73696B/en unknown
- 1973-01-31 FR FR7303304A patent/FR2171109B1/fr not_active Expired
- 1973-02-01 GB GB519773A patent/GB1415597A/en not_active Expired
- 1973-02-02 IL IL41450A patent/IL41450A/en unknown
- 1973-02-03 DE DE19732305320 patent/DE2305320B2/en not_active Ceased
- 1973-02-09 CA CA163,426A patent/CA1007586A/en not_active Expired
- 1973-02-09 NL NL7301897A patent/NL7301897A/xx unknown
- 1973-02-09 AU AU52037/73A patent/AU469805B2/en not_active Expired
- 1973-02-09 IT IT20222/73A patent/IT987011B/en active
- 1973-02-10 ES ES411763A patent/ES411763A1/en not_active Expired
- 1973-02-11 JP JP48017002A patent/JPS4892446A/ja active Pending
-
1974
- 1974-05-17 FR FR7417192A patent/FR2245668A1/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| IL41450A0 (en) | 1973-04-30 |
| DE2305320B2 (en) | 1976-09-30 |
| CA1007586A (en) | 1977-03-29 |
| DE2305320A1 (en) | 1973-08-30 |
| FR2245668A1 (en) | 1975-04-25 |
| BE795316A (en) | 1973-05-29 |
| GB1415597A (en) | 1975-11-26 |
| IT987011B (en) | 1975-02-20 |
| FR2171109B1 (en) | 1979-03-02 |
| AU5203773A (en) | 1974-08-15 |
| FR2171109A1 (en) | 1973-09-21 |
| ZA73696B (en) | 1974-09-25 |
| NL7301897A (en) | 1973-08-14 |
| ES411763A1 (en) | 1976-01-01 |
| AU469805B2 (en) | 1976-02-26 |
| JPS4892446A (en) | 1973-11-30 |
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