IE912247A1 - Novel, protracted insulin analogues - Google Patents

Novel, protracted insulin analogues

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Publication number
IE912247A1
IE912247A1 IE224791A IE224791A IE912247A1 IE 912247 A1 IE912247 A1 IE 912247A1 IE 224791 A IE224791 A IE 224791A IE 224791 A IE224791 A IE 224791A IE 912247 A1 IE912247 A1 IE 912247A1
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lys
arg
pro
cys
insulin
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IE224791A
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Novo Nordisk As
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/62Insulins
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
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  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Diabetes (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Human insulin analogues wherein at least one of the amino acid residues in position B1-B6 has been replaced by a Lys or Arg have a prolonged insulin action. Asn in position A21 may be replaced by another amino acid residue to increase the stability of the insulin analogue in acid solution. Furthermore B30 may be blocked by means of an amido or ester group.

Description

The present invention relates to novel insulin analogues with a prolonged insulin action, to a process for 5 the preparation of such insulin analogues and to injectable solutions containing the novel insulin analogues.
Background of the invention Insulin analogues with a protracted insulin action have previously been described in EP 0194864A and EP 0254516A.
In EP 0194864A protracted human insulin analogues wherein the C-terminal carboxyl group of the B-chain is blocked with an amido or ester residue and the amino acid residue in position A4, A17, B13 and B21 may be substituted by Gin are described. EP 0254516A describes human insulin of the same type as in EP 0194486A but further being modified in the A21 position.
Some of the above insulin analogues may, however, show a too low biological potency or the level of prolongation may be too low for specific purposes.
It is the purpose of the present invention to develop novel insulin analogues with prolonged insulin action not suffering from the above-mentioned drawbacks.
Summary of the invention It has according to the present invention surprisingly been found that introduction of a positive charge in the N-terminal end of the B-chain gives rise to insulin analogues with a highly prolonged insulin action as compared to human insulin and also a high in vivo potency.
In its broadest aspect the present invention is thus related to novel analogues of human insulin wherein at least one of the amino acid residues from Bl to B6 has been 1991-06-14, ATS/VMN 3348.200-IE replaced by a basic amino acid residue, i.e. a lysine or arginine residue (Lys or Arg).
For the purpose of improving the stability of the novel insulin analogues asparagine (Asn) in position A21 may furthermore be substituted with another amino acid residue.
Also a further positive charge may be introduced by blocking the C-terminal carboxyl group in position B30 preferably by means of an amido or ester group.
If the present insulin analogues are prepared by the socalled transpeptidition method (for details see later) it might furthermore be an advantage that the amino group linked to the C-terminal end of the Lys or Arg residue substituent is a proline residue.
The invention is also related to a method for the preparation of the novel insulin analogues by which a biosynthetic precursor of the insulin analogue is converted into the insulin analogues by enzymatic and chemical conversion and to insulin solutions containing the novel insulin analogues.
By insulin analogues as used herein is meant a compound having a molecular structure similar to that of insulin including the disulphide bridges between CysA7 and Cys®7, and between CysA2° and Cys®·*·9 and an internal disulphide bridge between CysA6 and CysAH and with insulin activity.
Detailed description of the invention The present insulin analogues may be represented by the following formula I 1991-06-14, ATS/VMN 3348.200-IE p-sn A(l-6)-Cys-A(8-19)-Cys-Z S S (I) X,-X9-X3-X4-X5-Xfi-Cys-B(8-18)-Cys-B(20-29)-Y wherein Z is Asn or another naturally occuring amino acid residue, X^ is Phe, Lys or Arg, X2 is Val, Lys or Arg, X-j is Asn, Lys, Arg or Pro, X4 is Gin, Lys, Arg or Pro, X5 is His, Lys, Arg or Pro, X6 is Lys, Arg, Leu or Pro and Y is a threonine residue wherein the carboxyl group may be blocked by an ester or amido group, with the proviso that at least one of χχ, X2, X3, X4, X5 and X6 is Lys or Arg.
Compared with human insulin, the change in charge is obtained by substituting one or more of the amino acid residues in position BI to B6 with an arginine or lysine residue. In addition, the C-terminal carboxyl group of the Bchain may be blocked by an ester group or amide group.
If Z is not asparagine it may be a neutral amino acid, for example valine, glutamine, isoleucine, leucine, phenylalanine, tyrosine, methionine or preferably glycine, serine, threonine or alanine. Z may also be an acidic amino acid, viz. glutamic acid or aspartic acid, or a basic amino acid, viz. lysine, arginine or histidine. Z is preferably glycine, alanine or serine.
Examples of blocking groups of the C-terminal carboxyl group in the B30 amino acid residue (threonine) are ester moities such as lower alkoxy with preferably not more than 8 carbon atoms, preferably less than 5 carbon atoms. Preferred alkoxy groups are methoxy, ethoxy and tertiary butoxy.
The blocking group may also be an amido group with the formula -NR^R^ wherein R^ and are the same or 1991-06-14, ATS/VMN 3348.200-IE different and each represents hydrogen or alkyl with preferably up to 8 carbon atoms. R1 and R2 are preferably each hydrogen.
Since compounds of formula I can be applied in the 5 clinic as solutions having a prolonged action, a decline in immunogenicity as compared to the commonly used suspensions of porcine or human insulins may occur.
The degree of prolongation can be enhanced and controlled by the addition of zinc ions.
Parameters that may control the degree of prolongation of the insulin effect are the concentration of zinc and the choice of the compound of formula I. The range for preferred zinc content extends from 0 to about 2 mg/ml, preferably from 0 to 200 ^ig/ml zinc and more preferably from about 20 to 200 /xg/ml in a preparation containing about 240 nmole of a compound of formula I per ml. Using other concentrations of the compound of formula I, the content of zinc is to be adjusted correspondingly.
The prolonged action of solutions of compounds of formula I in the presence of zinc ions is ascribed to the low solubility of such compounds at neutral pH.
The pH of the injectable solution of this invention should preferably be below the physiological pH, the upper limit being the pH where precipitation occurs. At the physiological pH value, compounds of formula I of this invention have a low solubility. Stable solutions containing about 240 nmole/ml of compounds of formula I per ml have been obtained at pH about 5.5. The upper limit depends upon the constituents of the solution, i.e. isotonikum, preservative and zinc concentration, and upon the choice of compound of formula I. There is no lower pH limit of the solutions and the chemical stability of the compounds of formula I where Z is different from asparagine, is high, even at pH 3. The preferred pH range for the injectable solutions of this invention is from about 2.5 to 8.5, more preferred from about 1991-06-14, ATS/VMN 3348.200-IE 4.5 to 8. Especially preferred are pH ranges about 2.5 to 5.5, most prefered about 3 to 4.5.
A furter aspect of this invention is that it provides improved flexibility for the patients. With two aqueous solutions, one containing a compound of formula I and the other containing a zinc salt, the patient can obtain a desired degree of prolonged action and a desired profile by mixing the two solutions appropriately. Thus, the patient has, using two stock solutions, the possibility of choosing one action and profile for the morning injection and another action and profile for the evening injection. Preferably, the zinc solution of this invention contains between about 2 pg and 2 0 mg zinc per ml. Alternatively, both of the stock solutions may contain zinc, either in the same or different concentrations, and/or both the stock solutions may contain a compound of formula I, either the same or different compounds.
Perferably, the injectable solutions of this invention have a strength of between about 60 and 6000 nmole of the compound of formula I per ml.
Examples of novel insulin analogues according to the present invention are ArgB5,SerA21,ThrB30-NH2 human insulin ArgB5,ProB6,SerA21,ThrB30-NH2 human insulin ArgB5,GlyA21,ThrB30-NH2 human insulin ArgB5,ProB6,GlyA21,ThrB30-NH2 human insulin ArgB2,SerA21,ThrB30-NH2 human insulin ArgB2,ProB3,SerA21,ThrB30-NH2 human insulin ArgB2,GlyA21,ThrB30-NH2 human insulin ArgB2,ProB3,GlyA21,ThrB30-NH2 human insulin ArgB2,ArgB3,SerA21,ThrB30-NH2 human insulin ArgB2,ArgB3,Ser^21 human insulin ArgB4,ProB5,SerA21,ThrB30-NH2 human insulin ArgB4,ArgB5,ProB6,GlyA21,ThrB3° human insulin 1991-06-14, ATS/VMN 3348.200-IE ArgB3,GiyA21,ThrB30-NH2 human ArgB3,SerA21,ThrB30-NH2 human ArgB4,GlyA21,ThrB30-NH2 human ArgB4,SerA21,ThrB30-NH2 human ArgB1,ProB2,GlyA21,ThrB3 °-NH2 insulin insulin insulin insulin human insulin.
The novel insulin analogues according to the present invention may be prepared by altering the proinsulin gene through replacement of codon(s) at the appropriate site in the native human proinsulin gene by codon(s) encoding the desired amino acid residue substitute(s) or by synthesizing the whole DNA-sequence encoding the desired insulin analogue. The gene encoding the desired insulin analogue is then inserted into a suitable expression vector which when transferred to a suitable host organism, e.g. E. coli.
Bacillus or yeast, generates the desired product. The expressed product is then isolated from the cells or the culture broth depending on whether the expressed product is secreted from the cells or not.
The novel insulin analogues may also be prepared by chemical synthesis by methods analogue to the method described by Marki et al. (Hoppe-Seyler1s Z. Physiol.Chem., 360 (1979), 1619-1632). They may also be formed from separately in vitro prepared A- and B-chains containing the appropriate amino acid residue substitutions, whereupon the modified A- and B-chains are linked together by establishing disulphide bridges according to known methods (e.g. Chance et al., In: Rick DH, Gross E (eds) Peptides: Synthesis Structure - Function. Proceedings of the seventh American peptide symposium, Illinois, pp. 721-728).
The insulin analogues may furthermore be prepared by a method analogue to the method described in EP 0163529A, the disclosure of which is incorporated by reference hereinto. By such a method an insulin precursor of human insulin wherein LysB29 is connected to GlyA1 by means of either a peptide 1991-06-14, ATS/VMN 3348.200-IE bond or a peptide chain of varying length with correctly positioned disulphide bridges is expressed and secreted by yeast and then converted into human insulin by the so-called transpeptidation reaction.
The transpeptidation reaction is described in US patent specification No. 4,343,898 (the disclosure of which is incorporated by reference hereinto). In this reaction the peptide bond or peptide chain connecting Lys®29 and Gly^I is exised and a threonine ester or threonine amide group is coupled to the C-terminal end of Lys®29.
The novel insulin analogues may thus be prepared by a method wherein a biosynthetic insulin precursor with the following formula II (Qq-T)rAl-A(2-6)-Cys-A(8-19)-Cys-Z (II) s s I I s s I I X1-X2-X3-X4-X5-X6-Cys-B(8-18)-Cys-B(20-28)-B29 wherein Q is a peptide chain with q amino acid residues, q is 25 an interger from 0 to 33, T is Lys or Arg, r is 0 or 1 and X-p X2, X3, X4, X5, Xg and Z are defined as above, is reacted with a compound of the formula III HY (III) wherein Y is a protected threonine amino acid wherein the 30 carboxyl group is protected with an ester or amido group, using trypsin or trypsin like enzymes as a catalyst in a mixture of water and organic solvent. The ester or amido protecting group may then be cleaved off by acid or basic hydrolysis. 1991-06-14, ATS/VMN 3348.200-IE Preferred compounds of formula III are Thr-NH2, Lys(Boc)-NH2, Thr(But)-0But and Thr-0Bufc.
Insulin preparations of this invention are prepared by dissolving a compound of formula I in an aqueous medium at slightly acidic conditions, for example, in a concentration of 240 or 600 nmole/ml. The aqueous medium is made isotonic, for example, with sodium chloride or glycerol. Furthermore, the aqueous medium may contain zinc ions in a concentraion of up to about 30 /ig of Zn++ per nmol of compound of formula I, buffers such as acetate, citrate and histidine and preservatives such as m-cresol, nipagin or phenol. The pH value of the final insulin preparation depends upon the number of charges that have been changed in the compound of formula I, the concentration of zinc ions, the concentration of the compound of formula I and the compound of formula I selected. The pH value is adjusted to a value convenient for administration such as about 2.5 - 5.5, preventing precipitation. The insulin preparation is made sterile by sterile filtration.
The insulin preparations of this invention can be used similarly to the use of the known insulin preparations.
Terminology The abbreviations used for the amino acids are those stated in J.Biol.Chem. 243 (1968), 3558. The amino acids are in the L configuration. Unless otherwise indicated, the species of insulins stated herein is human.
As used in the following text B(l-29) means a shortened B-chain of human insulin from Phe®1 to Lys®29 and A(l-21) means the A-chain of human insulin.
The substitution(s) made in the huamn insulin molecule according to the practice of the invention is (are) indicated with a prefix referenced to human insulin. As an example Arg®2 human insulin means a human insulin analogue wherein Arg has been substituted for Val in position 2 in the 1991-06-14, ATS/VMN 3348.200-IE B-chain. ArgB2,B(l-29)-Ala-Ala-Lys-A(l-21) human insulin means a precursor for the forementioned insulin analogue wherein Arg has been substituted for Val in position 2 in the shortened B-chain and wherein the B(l-29) chain and the A(l5 21) chain are connected by the peptide sequence Ala-Ala-Lys.
Unless otherwise stated it is to be understood that the B(l-29) chain and the A(l-21) chain are connected by disulphide bridges between CysA7 and Cys®7 and between CysA2° and Cys®19, respectively, and that the A-chain contains an internal disulphide bridge between CysA6 and CysA1, as in human insulin.
Experimental part Example 1 ArgB2,ProB3,SerA21,ThrB30-NH2 human insulin, ArgB5,SerA21,ThrB30-NH2 human insulin, ArgB4,GlyA21,ThrB30-NH2 human insulin and ArgB2,Pro®3,GlyA21,ThrB30 human insulin ArgB3,GlyA21,ThrB30-NH2 human insulin The above compounds were synthesized from the corresponding the following precursors: ArgB2, ProB3, Ser^^BC 1-29) -Ala-Ala-Lys-A(1-21) human insulin, ArgB5,Ser^^Bf 1-29) -Ala-Ala-Lys-A( 1-21) human insulin, ArgB4,GlyA21,B(l-29)-Ala-Ala-Lys-A(l-21) human insulin, ArgB2,Pro®3,Gly^-^Bf 1-29)-Ala-Ala-Lys-A( 1-21) human insulin, and ArgB3,GluA21,B(l-29)-Ala-Ala-Lys-A(l-21) human insulin by tryptic transpeptidation in organic/aqueous solution in the presence of Thr-NH2 as described in EP 0194864A, Examples 4 and 6. Yields, charges relative to human insulin, rates of migration relative to insulin in DISC PAGE electrophoresis at pH 8.9 and deviations in amino acid compositions from human insulin appear from Table I. 1991-06-14, ATS/VMN 3348.200-IE The insulin precursors were produced by a method analogous to the method described in EP 0163529A.
The insulin precursors were recovered from the fermentation broths by adsorption of LiChroprep™ RP-18 as described in Example 7 of EP 0163529A. The precursors were eluted from the column with 0.2 M KC1, 0.001 M HC1 in 33% (v/v) ethanol. The insulin precursors were crystallized from the pool by successive additions of water (1 volume per volume of pool), solid trisodium citrate to obtain a molarity of 0.05 M and finally zinc acetate to obtain a molarity of 0.006 M. The pH value was adjusted to 6.8 and the mixture was left overnight at 4 °C. The crystals were isolated by centrifugation, washed with water and dried in vacuo.
Protected amino acids and protected peptides for enzymatic semisynthesis were either prepared by standard methods or purchased (custom synthesis) from either Nova Biochem or Bachem, both Switzerland. 1991-06-14, ATS/VMN 3348.200-IE Cd >1 V rd co 0 0 c C rd {—I •rl in -η i + + e CHO (0 0 3 Cj * •h in 3 0 rd Cj in >1 ft o ft C Jj C a) Cj (0 Φ •rl i—l in Cj in rl •Η -rd X3 rd CP > CO S3 0 CP <3 tn o in 0 C3 β rd i—1 i—l i—I rd rd i—I cn c Οι (β ·Η + 1 + 1 + 1 + 1 0 B e ο in •rl 0 3 rt φ —. +J 0 X3 3 &> ft tP ft tP 3 IP rd (0 Cd W Ό μ in Cd in Cj r-H Cd 10 •H Ό β Φ -H •H < < 0 > > •η ο -p in (0 Φ o kp >tn) rd cn rd rd r-1 rd rd rd Q Ifl P (1) H μ + 1 + 1 + 1 + 1 Table •rd +J «3 0 C Cd 4-1 •rd tP rd •H Φ 3 β IP > in . •H c MH co 4-> •rd 0 (0 S3 rd c Φ ft Φ <0 P Cd β <0 •P 3 X (0 •C Φ f- > •rl X -P ft 10 rd P Φ (0 Cd C <0 C Φ β -rd tp 3 rd Cj x3 3 (0 in Λ 0 c U 4J •H in in in in in in in in cn cn cn + + + CN + n· 'f oo r-~ Ό 04 in in rd Φ <#> •rd >· rd rd CN CN C ίΐ >1 rl Φ rd c CO * 0 c -rd rd rd o rd n 04 CN r> •rd 3 X CN id CN < CN X CN P in 0 X X >1 X o X 3 c Cd X Φ X rd X Cj X -P •rd CP 1 co 1 0 CP 1 •rd o o *. o o 4J c CN m in cn M· cn CN cn in 10 CQ X m X X X X X 43 g tP Cd tp Cd tP Cj IP Cj 3 3 Cd X3 Cj X3 Cd X3 Cj X3 cn X3 Ed Ed EJ < Ej in o in rd rd 1991-06-14, ATS/VMN 3348.200-IE Sterile injectable solutions of the above compounds for testing of the degree of prolonged action were made using 1.6% (w/v) glycerol as the isotonicum, and 0.26% (w/v) phenol as the preservative. The concentration of zinc ions was 8, 80 or 160 ^g/ml. The pH values of the solutions were adjusted sufficiently off the isoelectric point of the analogues to keep the solutions clear upon storage at 4 °C. The solutions contained 240 nmole/ml of the tested compounds. The concentration of 240 nmole/ml was verified by HPLC.
Injectable solutions containing 240 nmole/ml of the compounds stated in Table II and having the pH of 3 and content of zinc stated therein were then made.
The prolongation of the hypoglycemic effect was tested according to British Pharmacopoeia 1980. A 142, in fasted rabbits. Each test solution was administered subcutaneously in a dosis of 14.3 nmole per rabbit in 12 animals weighing 3 - 4 kg, and the course of the hypoglycemia was followed for 6 hours. For comparison the fast acting preparation, Ac trap id™ human insulin was included in the tests. The results of the tests are shown in Table II giving the percentage of glucose after 1, 2, 4 and 6 hours (h) .
Results from determination of biological potencies Mouse Blood Glucose Assay (MBG) and Free Fat Cell Test (FFC) are listed in Table III. 1991-06-14, ATS/VMN 3348.200-IE Table II Compound Substitutions in human insulin 5 ArgB5,SerA21,ThrB30-NH2 ArgB5,Ser^21,ThrB3 °-NH2 ArgB5,Ser^21,ThrB3 °-NH2 ArgB2,ProB3,Ser^21,ThrB3 °-NH2 ArgB2,ProB3,Sei^21,ThrB3 °-NH2 Actrapid™ human insulin Zn++ Glucose in percent of initial Mg/ml lh 2h 4h 6h 8 59 64 96 94 80 85 92 97 95 160 76 89 96 94 8 58 67 73 72 80 73 66 64 61 7 53 53 92 97 3348.200-IE 1991-06-14, ATS/VMN Biological potency relative to human insulin. (British Pharmacopoeia 1980, A141 - A142).
N o o n ί—1 rH m X CM CM X c •H h X >1 Li X E-i Φ t—1 E-i in c w o C -H <—1 «. rH 0 i—1 η m OJ •H 3 j-ι in OQ oj O X CO m 0 X 5, g c φ P 2 Li 2 i—1 +j -H W Λ 1 X 1 U H •o •o 4-> C in m n oj n N· in io co CO co CO X X Λ E tp tP M tP M tP g 3 Μ X M X Li w X < E-i C Η <3 in in rd O OJ 1991-06-14, ATS/VMN 3348.200-IE

Claims (10)

1. Human insulin analogues having the following formula I r~ s n A(l-6)-Cys-A(8-19)-Cys-Z S S I I s s I I X 1 -X2-X 3 -X 4 -X 5 -X 6 -Cys-B(8-18)-Cys-B(20-29)-Y (I) wherein Z is Asn or another naturally occuring amino acid 15 residue, X^ is Phe, Lys or Arg, X 2 is Val, Pro, Lys or Arg, X 3 is Asn, Lys, Arg or Pro, X 4 is Gin, Lys, Arg or Pro, X 5 is His, Lys, Arg or Pro, Xg is Lys, Arg, Leu or Pro and Y is a threonine residue wherein the carboxyl group may be blocked by an ester or amido group, with the proviso that at least 20 one of X·^, X 2 , X 3 , X 4 , X 5 and Xg is Lys or Arg.
2. Human insulin analogues according to claim 1, wherein X 3 is Phe.
3. Human insulin analogues according to claim 1 or 2 wherein Z is Gly, Ala or Ser. 25
4. Human insulin analogues according to claim 1 wherein X 3 is Phe, X 2 is Arg or Lys, X 3 is Pro, X 4 is Gin, X 5 is His, Xg is Leu, Z is Gly, Ala or Ser and Y is Thr-NH 2 or Thr.
5. Human insulin analogues according to claim 1 30 wherein X 3 is Phe, X 2 is Val, X 3 is Asn, X 4 is Gin, X 5 is His, Xg is Arg or Lys, Z is Gly, Ala or Ser and Y is Thr-NH 2 or Thr.
6. Injectable solutions with prolonged insulin action containing a human insulin analogue with the general formula 35 I 1991-06-14, ATS/VMN 3348.200-IE r s_s n A(l-6)-Cys-A(8-19)-Cys-Z S S I I s s I I x l- X 2 -X 3“ X 4~ X 5 X 6“ C y s-B ( 8- l 8 ) _C Y S-B ( 20-29 ) -Y (I) 10 wherein Z is Asn or another naturally occuring amino acid, X·^ is Phe, Lys or Arg, X 2 is Val, Lys or Arg, X 3 is Asn, Lys, Arg or Pro, X 4 is Gin, Lys, Arg or Pro, X 5 is His, Lys, Arg or Pro, X 6 is Lys, Arg, Leu or Pro and Y is a threonine residue wherein the carboxyl group may be blocked by an ester 15 or amido group, with the proviso that at least one of χ χ , X 2 , X 3 , X 4 , X 5 and X 6 is Lys or Arg, together with conventional auxiliary agents, such as buffers, preservatives and agents making the solution isotonic.
7. A method for making insulin analogues with the 20 following formula I r s- s q A(l-6)-Cys-A(8-19)-Cys-Z S S I I s s I I X 1 -X 2 -X 3 -X 4 -X 5 -X 6 -Cys-B(8-18)-Cys-B(20-29)-Y (I) 30 wherein Z is Asn or another naturally occuring amino acid residue, X^ is Phe, Lys or Arg, X 2 is Val, Pro, Lys or Arg, X 3 is Asn, Lys, Arg or Pro, X 4 is Gin, Lys, Arg or Pro, X 5 is His, Lys, Arg or Pro, X 6 is Lys, Arg, Leu or Pro and Y is a threonine residue wherein the carboxyl group may be blocked 35 by an ester or amido group, with the proviso that at least 1991-06-14, ATS/VMN 3348.200-IE one of X lr X 2 , X 3 , X 4 , X 5 and X g is Lys or Arg, wherein an insulin percursor with the following formula II (Q q -T) r Al-A(2-6)-Cys-A(8-19)-Cys-Z ; ϊ (II) s s I I 10 s s I I X l -X 2“ X 3“ X 4 X 5 X 6“ C y s B ( 8 - 18 )-Cys-B(20-28)“ B2 9 wherein Q is a peptide chain with q amino acid residues, q is an interger from 0 to 33, T is Lys or Arg, r is 0 or 1 and 15 Xj, X 2 , X 3 , X 4 , X 5 , X 6 and Z are defined as above, is reacted with a compound of the formula III HY (HI) wherein Y is a protected threonine amino acid residue wherein the carboxyl group is protected with an ester or amido group, 20 using trypsin or trypsin like enzymes as a catalyst in a mixture of water and organic solvent, whereupon the protecting group if desired is cleaved off by acid or basic hydrolysis.
8. A method for making insulin analogues having the formula I as defined in claim 7 substantially as described herein with reference to the Examples.
9. Insulin analogues of formula I as defined in claim 7 whenever prepared by a method as claimed in claim 7 or claim 8.
10. An injectable solution comprising an insulin analogue as claimed in claim 9.
IE224791A 1990-06-28 1991-06-27 Novel, protracted insulin analogues IE912247A1 (en)

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