IE54490B1 - Pharmaceutical composition containing an aliphatic amino sulphonic acid - Google Patents

Description

This invention relates to a pharmaceutical composition for treating psoriasis.

Psoriasis is a group of disfiguring and uncomfortable skin conditions for which physicians have long sought effective treatment methods. Its causes and etiology are very imperfectly understood. The term psoriasis as used in this specification designates not only the known five or six conditions commonly so designated in medical practice, but also, for the sake of simplicity in terminology, other 10 related skin disorders namely the ichthyoses group, the dyskeratoses group and Dariers disease.

The principal known compositions which have been used with greater or lesser success in the treatment of psoriasis belong to two groups: 1) coal tar products, 2) cortisone products. Group 1) compositions are variable and unpredictable in their effects, but their overall success in symptom alleviation has been small. Some of them cause - undesirable skin discoloration. Group 2) compositions bring striking short-term benefit to sufferers, but are attended by well known undesirable side-effects which constitute a contraindication of extended periods of treatment.

It is an object of the invention to provide a pharmaceutical composition which will contribute to supplying the medical need mentioned above, and to provide certain sulfonic acid derivatives for use in treating psoriasis.

The invention accordingly provides a pharmaceutical composition for treating psoriasis which comprises at least one skin-compatible zwitterionic aminosulfonic acid (hereinafter ZASA) selected from: 2-(N-Morpholinyl)-ethane sulfonic acid (hereinafter MES) which has a pK& of 6.15; 2- [N-[N'-(2-Hydroxyethyl)]-piperazinyl]-ethane sulfonic acid (hereinafter HEPES) which has a pK of 7.55; a 3- [N-[N'-(2-Hydroxyethyl)]-piperazinyl]-propane sulfonic 15 acid (hereinafter HEPPS)? 2-[N-[tris-(Hydroxymethyl)]-methylamino]-ethane sulfonic acid; 2-(N-Piperazinyl)-ethane sulfonic acid; 2-(N-Piperazinyl)-propane sulfonic acid; Piperazine-l,4-bis(2-ethane sulfonic acid) (hereinafter PIPES) which has a pK_ of 6.8; and a N,N-bis-(2-Hydroxyethyl)-2-aminoethane sulfonic acid (hereinafter BES) which has a pK of 7.15, together with a a pharmaceutically acceptable topical carrier or base.

The invention also provides a zwitterionic aminosulfonic acid selected from the above group, for use in the preparation of a pharmaceutical composition for the topical treatment of psoriasis and the above-related skin disorders. The selected ZASA should have at least one pK value at 20°C in the range 6.0-8.3 to ensure comfort in its use on human skin (i.e. the molecule exists mainly in its dipolar form in the pH range 6.0-8.3). All pk _ cl values in this specification are at 20 c.

The ZASA's mentioned above are known in the chemical literature as buffers. Host of them are described among other compounds by Good, N. et al. in Biochemistry 1966, , 467.

Preferred ZASA's are mild and cause no skin irritation.

The effective proportion of the active ingredient, by weight of the composition, is in the range 0.05 to 20%, preferably 0.05 to 5%. In the most preferred compositions the effective proportion lies in the range 0.1% to 1.0%.

The topical base is selected from a wide variety of compositions formulated according to known principles for pharmaceutical purposes. Such compositions include creams, solids, ointments, lotions and film-forming solutions among others. They may be presented in boxes, jars or squeezable tubes, both collapsible and non20 collapsible. The solids may be presented as sticks for rubbing on to the skin. Some of the topical bases may be presented as papers, woven or non-woven fabric pieces, or pads, all impregnated with the composition.

The Invention will be appreciated in greater detail from the following examples of specific embodiments thereof.

EXAMPLE 1 A vanishing cream is made up A. OIL PHASE Stearic acid Microcrystal 1ine wax 01ive oi 1 Glyceryl monostearate (acid-stable grade) Polyoxyethylene sorbitan monolaurate^ Silicone fluid (200-350 mm /s The product sold under the B. AQUEOUS PHASE from the following recipe: PARTS BY WEIGHT 13.0 6.5 3.5 3.5 12.0 3.0 trade name TWEEN 20.

PARTS BY WEIGHT HEPES Water (q.s. ad 100.0) 0.2 58.3 The ingredients of A are melted together and brought to 80*C. The ingredients of B are made into a solution, brought to 80’C, and added at that temperature to the melt, with mixing, which is then continued until the emulsified mass has cooled to 40*C. The product is suitably packed so as to prevent evaporation, since it is an oil-in-water emulsion.

This cream is applied to an area of skin showing psoriatic symptoms, preferably several hours before the skin is due to be washed. The application may be repeated after washing.

EXAMPLE 2 An application stick is made from the following ingredients: PARTS BY HEIGHT Eutanol G 39 Comperlan HS^ 11 Stearic acid 10 HEPPS 5 Ethanol 96% v/v 20 Glycerol 15 ^Henkel International, Federal Republic of Germany.

The ingredients other than ethanol are mixed and melted together. The temperature of the melt is brought below 70*C, whereupon the ethanol is added and well mixed in.

The melt is poured into suitable moulds and allowed to set. The resulting moulded sticks are removed from the moulds, wrapped individually in aluminium foil and packed.

A stick is rubbed gently on to an area of skin showing psoriatic symptoms. These sticks may conveniently be carried in a patient’s pocket or handbag.

EXAMPLE 3 An ointment is made from the following ingredients: PARTS BY HEIGHT Liquid paraffin 11.0 Petroleum jelly 30.0 Paraffin wax 6.0 Glycerol 38.0 Polyoxyethylene homogeniser 10.0 HEPPS 5.0 The ingredients other than glycerol are melted together.

The temperature of the melt is brought below 70*C whereupon the glycerol is added with good stirring, the ointment is cooled to room temperature with further agitation, and when cool is passed once through a conventional ointment mill. This ointment is suitable for treating larger areas of skin, or where the cosmetic effect of treatment is deemed unimportant.

EXAMPLES 4 AND 5 These are creams of the vanishing type. They are made up from the following sets of ingredients: PARTS BY HEIGHT A. OIL PHASE Example 4 Example 5 Stearic acid 10.0 10.0 Beeswax 2.0 2.0 Paraffin wax 12.0 10.9 Polyoxyethylene sorbitan monolaurafe^ 10.9 10.0 Glycerol monostearate (acid stable grade) 5.0 5.0 D.C. Silicone fluid 200/350 (Dimethyl siloxane) — 2.5 B. AQUEOUS PHASE HEPES 1.0 1.0 Sorbitol 10.0 Magnesium sulphate 0.1 0.1 Water (g.s. ad 100) 49.0 58.5 TOTAL 100.0 100.0 Commercially available under the trade name TWEEN 20.

The ingredients of B are made into a solution, brought to 90*C-95’C, and added with stirring to the melted and mixed ingredients of A at about 80‘C. Stirring is continued until the temperature falls below 35’C or until the cream has set.

These creams are packed and used as described in Example 1.

EXAMPLES 6 AND 7 These vanishing creams contain as active ingredients at least one of the biological buffers described by Good, N. et al, Biochemistry 1966, £, 467. Of the zwitterionic buffer substances therein described, the ones here used have been found particularly suitable. Their names, as previously indicated, are herein abbreviated for convenience to MES and PIPES. The creams are made up from the following ingredients: PARTS BY HEIGHT OIL PHASE Example 6 Example 7 Stearic acid 10.5 10.5 Sunflower oil 3.5 3.5 Silicone fluid 4.0 4.0 Glycerol monostearate 5.0 5.0 Paraffin soft white 2.0 2.0 Tween 20 {see Ex.8-9) 10.0 10.0 Β. AQUEOUS PHASE MES PIPES Water (q.s. ad 100) TOTAL 4.0 61.0 100.0 0.1 64.9 100.0 The B ingredients are made into a solution and brought to 90*C-92’C, then added with stirring to a melt of the A ingredients made at 95‘C-l00*C and cooled to 90’C. Stirring is continued until the cream has set (below 35’C).

The creams are packed and used as described in Example 1. They can be removed from the hands by a simple soap and water wash, like the products of all the examples.

EXAMPLE 8 This example illustrates a typical formulation which can be used for the pharmaceutical presentation of any of the zwitterionic substances (ZASA's) the newly discovered medical properties of which are disclosed in the present speci fication.

INGREDIENT PARTS BY WEIGHT Stearic acid 10.0 Vegetable oil (e.g. Sunflower) 9.0 Glyceryl monostearate (selfemulsifying) 2.5 Silicone oil (200-350 mm2/s) 0.5 TWEEN 20 (Polyoxyethylene sorbitan monolaurate) 8.0 The selected ZASA 0.1 to 1.0 Water (q.s. ad 100) 69.9 to 69.0 The ingredients are put together by known pharmaceutical procedures, such as those set out in the previous examples.

In addition to preservatives, other conventional pharmaceutically acceptable additives may be incorporated in the compositions of the invention. These include, for example, humectants, film formers and water repellents. Sorbitol is a useful humectant. A 4% mucilage of Methyl cellulose is a useful film former. The dimethyl silicones sold by Imperial Chemical Industries Ltd., of the U.K. under the trade designations F 110 and F Ill, are useful water repellents.

The ZASA's herein prescribed, when used in proportions in the range 0.1 to 1.0% by weight of a pharmaceutical composition of the invention, are mild and cause no skin irritation. When applied regularly over a period of weeks to clean dry skin initially showing psoriatic symptoms, they have been shown firstly to ease the cracked, dry skin which is typical of the psoriatic condition, secondly to ease the concomitant irritation, and finally to arrest the pathological condition in a relatively rapid manner, permitting the skin to return to a normal, healthy-looking state free from redness, scale, chapping and cracking. The period involved is usually two to four weeks.

EXAMPLE 9 Therapeutic effect.

The effect was first observed by chance in a factory environment wherein compositions of the present invention were in use for a non-medical purpose (see Patent Application No. ,3 ) The tests of the present example were then organized ad hoc. ii Eight volunteer adult sufferers from mild to moderate plaque-type psoriasis were selected for treatment during respective active episodes of the ailment. Five were males, three females; their ages ranged from 16 to 50. At least five of them had had prior professional diagnosis by a family physician. None was receiving intensive topical or systemic treatment at the time of the investigation.

The body areas affected varied from one volunteer to another, but included the hands, forearms, elbows, face and, in one case, the scalp.

Each volunteer was provided with a quantity of the product of Example 8 containing, as active ingredient, HEPES in a proportion of 0.3% by weight, and was instructed to apply it to the affected areas of skin twice a day after washing. The instructions were carried out by all the volunteers.

Seven volunteers reported subjective improvement after periods varying from 7 to 14 days, and this was confirmed in all seven cases by lay observation, and in two cases by the family physician. One volunteer reported no noticeable change in symptoms for the better or the worse.

Four of the volunteers (2 male, 2 female) subsequently discontinued the treatment. All four reported a recurrence of symptoms after about 7 days. This was also confirmed by lay observation.

The remaining three volunteers have persisted with the treatment and have had no recurrence of symptoms with the last six months.

Several other workers in the same establishment, with no symptoms or history of inflammatory skin disease, applied the same product to their hands twice a day for an extended period (over two months), using it as a protective cream against accidental tissue adhesion by cyanoacrylate-type adhesives. None of these reported any deleterious effect, on the skin or otherwise. These results are preliminary and incomplete.

The same product was tested at the Biological Laboratories, Ballina, Co. Mayo, Ireland and found not to be a skin irritant.

Preliminary testing on animals is under way, in which a 1% Hydrocortisone cream is being used as a comparison, but the results are not yet to hand.

Application has been made, on the basis of these preliminary results, to the National Drugs Advisory Board of Ireland for approval for the setting up of systematic clinical tests.

Meanwhile the pharmacology of the ZASA's is under investigation in the Department of Clinical Medicine, Trinity College, Dublin.

While the number of sufferers treated to date with compositions of the invention is small, all of them have reported the relatively rapid improvement mentioned above.

The face, hands, arms and elbows have all exhibited the kind of recovery described.

Although we do not wish to be bound by the terms of any theory, we suspect that pH control of the skin, coupled with the use of an aminosulfonic acid, is one factor in producing the effects we have seen. A major factor appears to be the recently discovered fact that the active ingredients of the compositions are effective in suppressing the functioning of neutrophils, a variety of white blood corpuscle. There is prior evidence to suggest that neutrophils, which are known to infiltrate into psoriatic lesions, are at least partly responsible for the damage to epidermal cell membranes which is characteristic of psoriasis. The evidence is summarised and amplified in two papers by Μ M Young and F J Bloomfield: 1) Influence of lithium and fluoride on degranulation from human neutrophils in vitro: Inflammation, Vol. 6, No. 3, 1982, pp. 257-267. 2) Enhanced release of inflammatory mediators from lithiumstimulated neutrophils in psoriasis: British Journal of Dermatology (1983) 108, Paper 607/6288.

One of the authors (Bloomfield) is responsible for the discovery of neutrophil function suppression by ZASA Is; the work is unpublished.

Claims (6)

CLAIMS:

1. A pharmaceutical composition for treating psoriasis and related skin disorders of the ichthyoses and dyskeratoses group and Dariers disease and 5 related conditions which comprises at least one skincompatible zwitterionic aminosulfonic acid (one ZASA) selected from 2-(N-Morpholinyl)ethane sulfonic acid (hereinafter MES) which has a pK of 6.15; & lo 2-[N-[N‘-(2-Hydroxyethyl)]-piperazinyl]-ethane sulfonic acid (hereinafter HEPES) which has a pK of 7.55; a 3-[N-[N'-(2-Hydroxyethyl)]-piperazinyl]-propane sulfonic acid (hereinafter HEPPS); 2. -[N-[tris-(Hydroxymethyl)]-methylaminoj-ethane sulfonic 15 acid; 2-(N-Piperazinyl)-ethane sulfonic acid; 2-(N-Piperazinyl)-propane sulfonic acid; Piperazine-1,4-bis(2-ethane sulfonic acid) (hereinafter PIPES) which has a pK of 6.8; and a 20 N,N-bis-(2-Hydroxyethyl)-2-am1noethane sulfonic acid (hereinafter BES) which has a pK a of 7.15, together with a pharmaceutically acceptable topical carrier or base.

2. A pharmaceutical composition as claimed in claim 1 wherein the proportion of ZASA is in the range 0.05 to 20% 25 by weight of the composition.

3. A pharmaceutical, composition as claimed in claim 2 wherein the proportion is in the range 0.05 to 5%.

4. A pharmaceutical composition as claimed in claim 3 wherein the proportion is in the range 0.1 to 1.0%.

5. 5. Ose of a skin-compatible zwitterionic aminosulfonic acid (ZASA), as defined in claim 1, for the preparation of a pharmaceutical composition for the topical treatment of psoriasis and related skin disorders of the ichthyoses and dyskeratoses and Dariers disease.

6. 10 6. A pharmaceutical composition according to claim 1, substantially as hereinbefore described with particular reference to Examples 1 - 8 of the accompanying Examples.