IE45441B1 - Live influenza virus vaccine and preparation thereof - Google Patents
Live influenza virus vaccine and preparation thereofInfo
- Publication number
- IE45441B1 IE45441B1 IE1237/77A IE123777A IE45441B1 IE 45441 B1 IE45441 B1 IE 45441B1 IE 1237/77 A IE1237/77 A IE 1237/77A IE 123777 A IE123777 A IE 123777A IE 45441 B1 IE45441 B1 IE 45441B1
- Authority
- IE
- Ireland
- Prior art keywords
- influenza virus
- strain
- influenza
- virus
- vaccine
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/145—Orthomyxoviridae, e.g. influenza virus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/525—Virus
- A61K2039/5254—Virus avirulent or attenuated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/54—Medicinal preparations containing antigens or antibodies characterised by the route of administration
- A61K2039/541—Mucosal route
- A61K2039/543—Mucosal route intranasal
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Virology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Pulmonology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Attenuated vaccine against influenza contains as active ingredient a strain of attenuated H3N2 type influenza virus resistant to serum inhibitors. The strain is influenza virus strain P/76/7. Used as vaccine against influenza suitable for intranasal administration. Pref. a unit dose of the vaccine contains at least 107 DIO50 (infective dose of 50% of inoculated eggs) of the P/76/7 virus strain. The parent patent BE 819865 describes a method of obtaining stable strains of influenza virus type A (H3N2) which are resistant to serum inhibitors. Each year the serotype of influenza virus type A in the world differs slightly from the strains observed the previous year; this antigenic modification causing problems for immunisation against influenza type A. To obtain maximum effect the vaccinal antigen must be nearest to the dominant virus and thus influenza vaccines are periodically adapted to give maximum protection against these new strains, the present addition comprising such a modification.
Description
The present invention relates to an attenuated influenza virus vaccine effective on intranasal administration and to the preparation thereof and it represents an improvement in or modification of the invention described in
Patent Specification No. 40180.
A process for preparing stable influenza virus (H3N2) strains completely resistant to serum inhibitors and useful as intranasal vaccines has been described in our patent specification No. 40180. According to said specification, these strains are obtainable by passaging, in the allantoic cavity of embryonated chicken eggs in the presence of normal serum, a
Ιθ recombinant strain previously obtained from an H3N2 influenza virus strain and the A/PR8/34 influenza virus strain. In particular, a virus strain previously obtained by recombining the A/England/42/72 influenza virus strain with the influenza virus strain A/PR8/34 was rendered resistant to serum inhibitors and the resistant strain was used in the preparation of live vaccines.
It is known that, almost every year, the serotype of the type A influenza virus strains spreading in the world is slightly different from the serotype of the previously observed strains.
This antigenic modification causes a specific problem for immunization against type A influenza virus.
In order to obtain the greatest efficacy from a vaccinal antigen, one must be as close as possible to the dominant virus and therefore the influenza vaccines must be adapted at regular intervals to confer the strongest protection against these new strains.
The present invention relates to such improvetent. By submitting to three passages in the alk'toic cavity of embrvonated chicken's eggs and in the presence of normal guinea pig serum, according to the process described in Patent Specification 40130 an influenza virus strain (HgNg) previously obtained by recombing the A/PR8/34 influenza virus with the A/Victoria/3/75 influenza virus, there was obtained an influenza virus strain completely resistant to serum inhibitors, having the A/Victoria/3/75 serotype and valuable for vaccinal use.
The so-obtained strain, named RIT 4050, has been deposited in the Collection of viruses held by the WHO Collaborating Centre for Collection and Evaluation of Data on Comparative Virology at the Institut fur Medizinische Mikrobiologie, Infekcions-und Seukenmedizin der LudwigMaximillians Universitat at Murchen (Bundesrepublik Deutschland) with the designation P/76/7.
Thus, the present invention provides an attenuated influenza vaccine effective on nasal administration containing as active component an effective dose of an influenza virus strain (subtype H^N^) which is attenuated and resistant to serum inhibitors, wherein the serum inhibitor resistant strain is the P/76/7 influenza virus strain. A dosage unit of the vaccine contains at least 10 ΕΙΟςθ ^05β -jnfec-fc-fve ·;η gg^ of inoculated eggs) of virus.
The present invention also provides a process for preparing such an attenuated vaccine, which comprises incubating the P/76/7 serum inhibitors resistant influenza virus strain in the allantoic cavity of embryonated hen's eggs, and incubation being for a period of time sufficient to permit growth of a large amount of said virus and harvesting the resulting virus material which is preferably supplemented with a stabilizer-peptone is an example of stabilizer-and freeze-dried.
4S 441
For the administration, the Vaccine, which is preferably kept in freezedried form, is extemporaneously reconstituted either by addition of water or by addition of any other pharmaciutical diluent or composition known to the art for the preparation of nasal preparations such as drops or spray and inoculated in the nasopharynx. If desired, a second dose is inoculated one to two weeks after the first administration.
The type A influenza virus vaccine of this invention can be used in combination with any other live influenza virus vaccine - e.g. a type B influenza virus vaccine-administrable by nasal route.
The following examples illustrate the present invention but they should not be construed as limiting its scope.
EXAMPLE 1
Preparation of the recombinant virus strain
An influenza virus (HjNj) strain (designated RIT 4057 in our collection) sensitive to serum inhibitors obtained by recombining the A/PR8/34 strain with the A/Victoria/3/75 strain and cloned by one end-dilution passage is used as starting viral material. Different dilutions (i.e. 10\ 10“^, 10“3, 10^ and IO’5) of said starting viral material in normal saline with a titre of 107‘5EI0g0/ml are mixed with a same volume of sterile normal guinea pig serum (in normal saline) previously adjusted at the 1/2 dilution and then maintained for 10 minutes in a water bath at 75°C, homogenized and centrifuged at 1000 g for 30 minutes. The supernantant is used for the further step which consists of incubating the virus serum mixtures at room temperature (20°C) for one hour before inoculating 0.2 ml aliquots of said mixture into the allantoic cavity of embryonated hen's eggs.
The eggs are then further incubated for a period of time varying between 24 and 96 hours at 37°C.
At the end of this incubation period, the allantoic fluid is harvested and tested for the presnece of influenza virus by the hemjgluti nation method. The harvested virus produced by the inoculum of the highest virus dilution which shows hemagglutinating activity (i.e. virus dilution 10“3) is used for a second passage performed in the same operative conditions.
The harvested virus produced by the inoculum of the highest virus dilution
454 41 (i.e. TO9) with guinea pig normal serum and which shows hem-gglutinating activity is ued for a third passage performed in the same operative conditions.
The harvested virus produced by the inoculum of the highest virus dilution (i.e. 10 ) with guinea pig normal serum and which shows hemagglutinating activity has been designated P/76/7 and characterized as follows.
Inhi bi tors-resi stance
Fcr testing the re; stance against the inhibitors present in normal heated animal serum (horse, guinea pig and calf serums previously heated at 75°C for one hour), serial twofold dilutions of the heated serums were mixed with four hemagglutinating units of the parent strain and the modified virus. After incubation for one hour at room temperature chicken red blood cells were added and the serra dilutions giving an inhibition of hemagglutination were recorded. The results are shown in the following Table 1.
TABLE 1
Serum of
Serum dilution inhibiting RIT 4057 hemagglutination
P/76/7 guinea pig 20 horse
4000 £. 10 > 4000 < 10
Table 1 shows that the P/76/7 strain is completely resistant to the serum inhibitors.
Stability of the inhibitors-resistant cnaracter
The resistant character of the P/76/7 strain has been confirmed in laboratory animals (ferrets) : the virus isolated 3 and 6 days after
6 inoculation (10 ‘ ΕΙΟ^θ) was found resistant to serum inhibitors.
Absence of side effects
A first group of 4 ferrets was inoculated intranasally with 107’5EIDg0 of the Β/ΙΒΓ! strain. The temperature of the animals was taken daily during days p.i. No significant temperature rise was recorded using the criteria determined by the C.W. Potter & si. (Br.J.Exp. Pathol.53 : 153-67, 1972). u 7 6
A second group of 3 ferrets was inoculated intranasally with 10 EIDg0 of the RIT 4057 strain. Two animals showed significant temperature rise.
EXAMPLE 2
Preparation of the vaccine
The virus material of the last passage of the process described in
Example 1 is used as inoculum for the production of the seed lot for the manufacturing of the vaccine.
The allantoic fluids are harvested, pooled, sterility and safety tested, mixed with peptone in order to reach a final concentration of 5% of peptone.
Aliquots of 0.5 ml are distributed into 3 ml glass vials in order to obtain dosage units (i.e. at least 107 EIDg0) of virus. The product is then freezedried and the vials tightly stoppered.
For vaccine administration, the contents of one vial are reconstituted by adding 0.5 ml of a diluent such as water or saline or a 5% sucrose
2o solution and administered as drops in the nostrils.
EXAMPLE 3
Clinical trials
7.3
Dosage units (10 ‘ ΕΙΟςθ) of the vaccine obtained following the process described in Example 2 have been reconstituted by adding 0.5 ml of a sterile 25 555 sucrose solution and 5 drops per nostril were administered to 15 healthy volunteers (mean age : 22 years).
A second dose was inoculated to 8 of the 15 volunteers 7 days after the first administration.
ΑΠ the volunteers were examined daily to detect eventual influenza symptoms. Blood samples were collected before, and 2 to 3 weeks after inoculation to determine the number of seroconversions and the geometric mean of the hemagluti nation inhibition (HI) titre.
The results of the clinical trials are summarized in the following
Tables II and III.
TABLE II
SYMTOMS
NUMBER (duration in days) conjunctival reaction sore throat stuffy nose rhinorrhea (3) 1 (2) 6 (1) 1 (1)
TABLE III : Serology
Number of seroconversion/ number of vaccinated
12/15
8/8
Number of doses geometric mean titre HI (x)
ND (xx) ( x) titre HI before vaccination<20 (xx) ND : not determined
As showed in Table II and III, the influenza virus strian P/76/7 is attenuated for humans and induces a high rate of seroconversion.
Claims (6)
1. An attentuated influenza virus vaccine effective on intranasal administration comprising an effective amount of serum inhibitors resistant strain of H 3 N 2 influenza virus, wherein the serum inhibitors resistant strain 5 is the P/76/7 virus strain.
2. An attenuated influenza virus vaccine according to claim 1, wherein the effective amount is at least 10 7 EIDg 0 of the P/76/7 strain per dosage unit.
3. An attenuated influenza virus vaccine according to either of claims 1 10 add 2, wherein the vaccine is supplemented with a stabilizer and freezedried.
4. A process of preparing an influenza virus vaccine according to any of claims 1 to 3, which comprises incubating in the allantoic cavity of hen's embryonated eggs the influenza virus serum inhibitors resistant strain 15 P/76/7 and harvesting the resulting virus material.
5. A process according to claim 4, substantially as hereinbefore described in Example 2.
6. An attenuated influenza virus vaccine, when prepared by a process according to either of claims 4 and 5.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BE843094 | 1976-06-18 |
Publications (2)
Publication Number | Publication Date |
---|---|
IE45441L IE45441L (en) | 1977-12-18 |
IE45441B1 true IE45441B1 (en) | 1982-08-25 |
Family
ID=3861379
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IE1237/77A IE45441B1 (en) | 1976-06-18 | 1977-06-16 | Live influenza virus vaccine and preparation thereof |
Country Status (6)
Country | Link |
---|---|
JP (1) | JPS52156920A (en) |
AT (1) | AT351160B (en) |
DE (1) | DE2726837A1 (en) |
IE (1) | IE45441B1 (en) |
LU (1) | LU77552A1 (en) |
ZA (1) | ZA773594B (en) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS59163313A (en) * | 1983-03-09 | 1984-09-14 | Teijin Ltd | Peptide hormone composition for nasal administration |
-
1977
- 1977-06-14 DE DE19772726837 patent/DE2726837A1/en not_active Withdrawn
- 1977-06-15 ZA ZA00773594A patent/ZA773594B/en unknown
- 1977-06-15 AT AT423877A patent/AT351160B/en not_active IP Right Cessation
- 1977-06-16 LU LU77552A patent/LU77552A1/xx unknown
- 1977-06-16 IE IE1237/77A patent/IE45441B1/en unknown
- 1977-06-16 JP JP7195077A patent/JPS52156920A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
DE2726837A1 (en) | 1977-12-29 |
IE45441L (en) | 1977-12-18 |
ATA423877A (en) | 1978-12-15 |
AT351160B (en) | 1979-07-10 |
JPS52156920A (en) | 1977-12-27 |
LU77552A1 (en) | 1977-09-19 |
ZA773594B (en) | 1978-05-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Virelizier | Host defenses against influenza virus: the role of anti-hemagglutinin antibody | |
Wiktor et al. | Cultivation of rabies virus in human diploid cell strain WI-38 | |
Swayne et al. | Influence of virus strain and antigen mass on efficacy of H5 avian influenza inactivated vaccines | |
CA2250078C (en) | Processes for the replication of influenza viruses in cell culture, and the influenza viruses obtainable by the process | |
Coleman et al. | The HONG KONG/68 influenza A2 variant | |
US4009258A (en) | Influenza vaccine containing a recombinant, antigenically hybridized virus and method of using the same | |
Maassab et al. | Laboratory and clinical characteristics of attenuated strains of influenza virus | |
US4338296A (en) | Influenza virus and process of producing a vaccine therefrom | |
Cox et al. | Comparative studies of wild-type and cold-mutant (temperature-sensitive) influenza viruses: Nonrandom reassortment of genes during preparation of live virus vaccine candidates by recombination at 25° between recent H3N2 and H1N1 epidemic strains and cold-adapted A/Ann Arbor/6/60 | |
US4053583A (en) | Live newcastle disease virus vaccines | |
Fagiolo et al. | Humoral and cellular immune response to influenza virus vaccination in aged humans | |
US4235876A (en) | Live Newcastle disease virus vaccine | |
US4318903A (en) | Live influenza virus vaccine and the preparation thereof | |
US4029763A (en) | Influenza vaccine containing purified neuraminidase antigen and method of using the same | |
Maassab et al. | Hybrid formation of influenza virus at 25 | |
US3953592A (en) | Live influenza virus vaccines and preparation thereof | |
Reciilard | Cell-culture vaccines for veterinary use | |
CA1152895A (en) | Process for the preparation of novel influenza virus strains and influenza virus vaccines containing them | |
Morris et al. | Antigenic relationship of 1961–1963 A2 influenza viruses to prototype A2 1957 strain | |
Van Kirk et al. | Evaluation of low temperature grown influenza A2/Hong Kong virus in volunteers | |
US4206287A (en) | Vaccines and their preparation | |
US3962423A (en) | Live influenza type B virus vaccines and preparation thereof | |
AU558483B2 (en) | Temperature sensitive reassortant viruses and a vaccine against equine influenza | |
IE45441B1 (en) | Live influenza virus vaccine and preparation thereof | |
Macpherson et al. | Strain differences in the Newcastle disease virus |