HUT48378A - Method for detecting b2 content of blood plasma tromboxan by means of radioimmunology - Google Patents
Method for detecting b2 content of blood plasma tromboxan by means of radioimmunologyInfo
- Publication number
- HUT48378A HUT48378A HU107587A HU107587A HUT48378A HU T48378 A HUT48378 A HU T48378A HU 107587 A HU107587 A HU 107587A HU 107587 A HU107587 A HU 107587A HU T48378 A HUT48378 A HU T48378A
- Authority
- HU
- Hungary
- Prior art keywords
- txb2
- blood plasma
- radio
- samples
- contg
- Prior art date
Links
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Radio-immunological assaying of thromboxane B2 (=TxB2) content of blood plasma is based on the determn. of antigen-antibody bondings, due to changes in bonding measured, using a tracer cpd. contg. a radio-isotope (i.e. a radio ligand) and a specific antibody, in the presence of a sequence of different dilutions of a known TxB2 standard and the samples to be determined. A calibration graph is drawn based on the standard dilution sequence and related changes in bonding and is used during the determn. of the unknown samples by reading off the concentrations as a function of observed bonding changes. - The essence of the method is that high specific activity thromboxane - B2-tyronine-methyl-ester deriv. which contains iodine 125 isotope (1) is used as a radio-liquid. This liquid reacts with a specific antibody in a buffer soln. contg. 8-aniline-naphthalene -sulphonic acid and in the presence of the standard dilution sequence contg. known concns. of TxB2, and of TxB2 free blood plasma samples otherwise identical with the unknown samples, and finally in the presence of the blood plasma samples to be determined.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU107587A HU198570B (en) | 1987-03-12 | 1987-03-12 | Method for radioimmunologic determining tromboxane b2 contents of bllod plasma |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
HU107587A HU198570B (en) | 1987-03-12 | 1987-03-12 | Method for radioimmunologic determining tromboxane b2 contents of bllod plasma |
Publications (2)
Publication Number | Publication Date |
---|---|
HUT48378A true HUT48378A (en) | 1989-05-29 |
HU198570B HU198570B (en) | 1989-10-30 |
Family
ID=10952739
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU107587A HU198570B (en) | 1987-03-12 | 1987-03-12 | Method for radioimmunologic determining tromboxane b2 contents of bllod plasma |
Country Status (1)
Country | Link |
---|---|
HU (1) | HU198570B (en) |
-
1987
- 1987-03-12 HU HU107587A patent/HU198570B/en not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
HU198570B (en) | 1989-10-30 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
HU90 | Patent valid on 900628 | ||
HPC4 | Succession in title of patentee |
Owner name: IZOTOP INTEZET KFT., HU |
|
HMM4 | Cancellation of final prot. due to non-payment of fee | ||
MM4A | Lapse of definitive patent protection due to non-payment of fees |